##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933086.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	506896
Sequences flagged as poor quality	0
Sequence length	52
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58355362835769	33.0	31.0	34.0	31.0	34.0
2	32.93481108550866	34.0	31.0	34.0	31.0	34.0
3	32.83359900255674	34.0	31.0	34.0	31.0	34.0
4	36.35871658091601	37.0	37.0	37.0	35.0	37.0
5	36.3437154761529	37.0	37.0	37.0	35.0	37.0
6	36.423364950601304	37.0	37.0	37.0	35.0	37.0
7	36.566400208326755	37.0	37.0	37.0	35.0	37.0
8	36.498092310848776	37.0	37.0	37.0	35.0	37.0
9	38.35887045863451	39.0	39.0	39.0	37.0	39.0
10	38.07529355133992	39.0	39.0	39.0	35.0	39.0
11	37.88942307692308	39.0	38.0	39.0	35.0	39.0
12	37.72148330229475	39.0	38.0	39.0	35.0	39.0
13	37.69564960070705	39.0	38.0	39.0	35.0	39.0
14	39.00680415706575	41.0	39.0	41.0	35.0	41.0
15	39.10815038982356	41.0	39.0	41.0	35.0	41.0
16	39.216101527729556	41.0	39.0	41.0	35.0	41.0
17	39.20899158801806	41.0	39.0	41.0	35.0	41.0
18	38.93548380732931	40.0	39.0	41.0	35.0	41.0
19	38.70987145292131	40.0	37.0	41.0	35.0	41.0
20	38.43660435276664	40.0	35.0	41.0	35.0	41.0
21	38.37954531106973	40.0	35.0	41.0	35.0	41.0
22	38.31765095798744	40.0	35.0	41.0	35.0	41.0
23	38.266405732142296	40.0	35.0	41.0	35.0	41.0
24	38.18075502667214	40.0	35.0	41.0	35.0	41.0
25	38.060890202329475	40.0	35.0	41.0	35.0	41.0
26	37.938537688204285	40.0	35.0	41.0	35.0	41.0
27	37.80737074271646	40.0	35.0	41.0	35.0	41.0
28	37.6861170733247	40.0	35.0	41.0	34.0	41.0
29	37.56084482813043	40.0	35.0	41.0	34.0	41.0
30	37.43346958744989	40.0	35.0	41.0	34.0	41.0
31	37.23681386319876	40.0	35.0	41.0	33.0	41.0
32	36.93718238060667	39.0	35.0	41.0	33.0	41.0
33	36.58264811716802	39.0	35.0	41.0	32.0	41.0
34	36.2429591079827	39.0	35.0	41.0	31.0	41.0
35	36.02991737950191	39.0	35.0	41.0	30.0	41.0
36	35.91947066064834	39.0	35.0	41.0	30.0	41.0
37	35.7818112591143	39.0	35.0	41.0	29.0	41.0
38	35.725673510937156	39.0	35.0	41.0	29.0	41.0
39	35.65077649064108	39.0	35.0	41.0	27.0	41.0
40	35.600576844165275	39.0	35.0	41.0	27.0	41.0
41	35.47025819892049	38.0	35.0	41.0	25.0	41.0
42	35.433582036551876	38.0	35.0	41.0	25.0	41.0
43	35.36202297907263	38.0	35.0	41.0	25.0	41.0
44	35.30888781919763	38.0	35.0	41.0	24.0	41.0
45	35.24966659827657	38.0	35.0	41.0	24.0	41.0
46	35.174029781256905	38.0	35.0	41.0	24.0	41.0
47	35.09389500015782	38.0	35.0	41.0	23.0	41.0
48	34.98919107667056	37.0	35.0	41.0	23.0	41.0
49	34.928470534389696	37.0	35.0	41.0	24.0	41.0
50	34.82923124270067	37.0	35.0	41.0	23.0	41.0
51	34.73756549666993	37.0	35.0	40.0	23.0	41.0
52	34.537960449480764	36.0	35.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	7.0
15	10.0
16	30.0
17	53.0
18	105.0
19	227.0
20	398.0
21	654.0
22	1041.0
23	1663.0
24	3059.0
25	5645.0
26	8487.0
27	9444.0
28	8833.0
29	8009.0
30	7774.0
31	8410.0
32	9690.0
33	12235.0
34	25042.0
35	41488.0
36	54130.0
37	42991.0
38	51873.0
39	204079.0
40	1515.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.419849436570818	20.495131151163157	23.509556200877498	25.575463211388527
2	42.58013478109908	20.930723462011933	23.32746756731164	13.161674189577349
3	24.27914207253559	21.34205044032701	40.64127552791894	13.73753195921846
4	21.829132603137527	24.560462106625423	40.07153341119283	13.538871879044223
5	21.826370695369466	29.476263375524763	35.776964110981346	12.920401818124427
6	24.763067769325463	34.566853950317224	31.725048136106814	8.945030144250497
7	74.78614942710142	1.9990293866986524	20.069797354881473	3.145023831318456
8	73.01142640699473	2.7244247340677377	20.68984564881159	3.5743032101259433
9	68.33433288090653	4.808678703323759	22.431820333954107	4.4251680818156
10	30.348828951106345	33.94108456172469	25.607225150721252	10.102861336447713
11	22.852024872952242	22.900358258893345	39.930873394147916	14.316743474006502
12	23.237902843975885	19.784334459139547	37.632177014614435	19.34558568227013
13	23.79876740001894	20.750015782330102	39.60516555664279	15.846051261008176
14	15.090472207316688	24.20417600454531	41.23252106941069	19.472830718727312
15	12.003448439127553	31.55755815788643	40.39211199141442	16.046881411571604
16	18.9796329030018	32.119014551308354	36.2551687131088	12.646183832581043
17	18.735401344654527	30.583788390517974	35.12475931946592	15.556050945361575
18	14.994002714560779	30.728985827467568	38.39683090811528	15.880180549856382
19	14.927519649000978	32.869661626842586	35.54200467156971	16.660814052586723
20	14.530396767778795	35.57929042643856	35.32618130740823	14.564131498374419
21	17.312624285849562	32.34095356838483	37.50966667718822	12.836755468577381
22	16.57164388750355	28.936507685994762	38.39762002462043	16.094228401881253
23	14.020430226318615	29.665059499384487	39.26268899340299	17.05182128089391
24	14.475158612417538	30.37526435402923	37.97662636911714	17.172950664436097
25	16.518575802531483	33.17840345948676	34.77162968340646	15.531391054575296
26	15.886296202771376	34.527792683311766	32.697239670464946	16.888671443451912
27	16.50160979767053	31.782259082730974	34.54041854739434	17.175712572204162
28	14.068566333133422	32.54967488399988	34.36464284586977	19.01711593699694
29	14.902465199962123	31.36777563839525	34.520690634765316	19.209068526877306
30	17.1930731353177	31.51100028408194	33.52620655913639	17.769720021463968
31	16.31399734856854	33.69843123638774	32.61260692528645	17.374964489757268
32	17.610121208295194	32.9700767021243	31.48101385688583	17.938788232694673
33	17.8074003345854	33.06319244973328	31.672374609387333	17.457032606293993
34	16.07035762760014	34.46643887503551	32.25809633534295	17.205107162021402
35	16.27592247719453	36.14035226160791	29.701753416874467	17.881971844323097
36	17.650957987437266	36.688393674442096	29.700372462990437	15.960275875130204
37	17.00348789495281	37.019428048357064	30.353958208389887	15.623125848300242
38	17.95417600454531	35.28159622486664	30.208563492314006	16.555664278274044
39	16.624317414223036	36.60494460402134	29.79289637322054	16.977841608535087
40	18.426856791136643	35.18887503551024	30.206787980177392	16.177480193175718
41	16.640691581705124	35.05768441652726	30.065930684006187	18.235693317761434
42	16.027350778068875	35.75269877844765	31.026285470786906	17.193664972696567
43	16.305711625264355	34.853105962564314	32.235803794072154	16.605378618099177
44	17.695543069978854	34.0920030933367	30.32081531517313	17.891638521511315
45	16.280854455351786	33.70277137716612	30.321604431678296	19.694769735803792
46	17.36786244121082	33.9389144913355	29.926651620845302	18.766571446608378
47	16.32169123449386	34.04189419525899	31.53881664088886	18.09759792935829
48	15.88905811053944	34.43763612259714	31.580442536536097	18.092863230327325
49	16.991651147375396	34.125737823932326	30.812434897888323	18.070176130803954
50	16.182806729585554	32.979348821059936	31.98683753669392	18.851006912660583
51	15.731432088633564	33.204641583283355	31.518102332628388	19.54582399545469
52	15.877418642088317	33.886832801994885	31.73175562640068	18.503992929516112
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	884.0
1	1260.5
2	1637.0
3	19203.0
4	36769.0
5	20839.5
6	4910.0
7	5249.0
8	5588.0
9	7165.5
10	8743.0
11	10596.5
12	12450.0
13	13225.5
14	13335.0
15	12669.0
16	11389.0
17	10109.0
18	9378.0
19	8647.0
20	7963.0
21	7279.0
22	7207.5
23	7136.0
24	7003.5
25	6871.0
26	7359.0
27	7847.0
28	8341.5
29	8836.0
30	9373.5
31	9911.0
32	10305.0
33	10699.0
34	11928.5
35	13158.0
36	13619.0
37	14080.0
38	14962.5
39	17273.0
40	18701.0
41	19703.0
42	20705.0
43	22425.5
44	24146.0
45	27437.5
46	30729.0
47	34622.5
48	38516.0
49	37066.5
50	35617.0
51	32284.5
52	28952.0
53	25884.0
54	22816.0
55	20480.0
56	18144.0
57	16216.5
58	14289.0
59	13219.0
60	12149.0
61	10282.5
62	8416.0
63	7077.5
64	4810.5
65	3882.0
66	3280.5
67	2679.0
68	2059.0
69	1439.0
70	1152.5
71	866.0
72	654.0
73	442.0
74	368.0
75	294.0
76	213.5
77	133.0
78	110.5
79	88.0
80	67.0
81	46.0
82	32.0
83	18.0
84	14.0
85	10.0
86	9.0
87	8.0
88	5.5
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	506896.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.835016350623285
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.0574474275571	37.138765345146666
2	13.332822407120867	13.555484901318914
3	5.549824245969572	8.463762188608461
4	2.8358590045800334	5.76643755463553
5	1.6081225296054367	4.087446754314902
6	0.9831865305006043	2.9988182012226483
7	0.6605411873662321	2.3505035442015774
8	0.43876596427938325	1.784373997459155
9	0.3055174753594883	1.3977887269770661
>10	1.1850980946035201	9.289932651098127
>50	0.019850652623463215	0.7065736394542046
>100	0.015569139277491501	1.6363382773300312
>500	0.002335370891623725	0.8830686495434013
>1k	0.00467074178324745	3.7426044154410567
>5k	0.0	0.0
>10k+	3.892284819372875E-4	6.198101153248273
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31325	6.179768631040687	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTC	2337	0.4610413181402102	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCG	2167	0.42750386667087525	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTT	1978	0.3902181118020264	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT	1881	0.3710820365518765	No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	1724	0.3401092137243143	Illumina Single End Adapter 1 (95% over 21bp)
GCTGAGGCTGACATAACTCTTTTCATGTTTAATTCTTGTCCCTTTAGTAGGG	1606	0.31683027682207	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCT	1409	0.27796628894289954	No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCTT	1330	0.2623812379659733	Illumina Single End Adapter 1 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	1250	0.24659890786275684	Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCC	1103	0.21759887629809663	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTC	1083	0.21365329377229253	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	1047	0.20655124522584514	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGT	915	0.18051040055553802	No Hit
GCCATGGATGTTATGGTCAGCTTCGGATTGAAGTCGAGTCAAATTTCAAAAC	823	0.16236072093683912	No Hit
CGTCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG	776	0.15308860200119948	No Hit
CACCGGCTTCAGCTCTACAGCCAGAAACAGCTGGAATGGGTCACTCAAATCC	742	0.14638111170733248	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	695	0.1371089927716928	No Hit
GCCAGATAAGACCTCTTCAGCTCCTTCAGTCCTTCCCCTAATTCCTCCATTG	512	0.1010069126605852	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.27461254379596606	0.0
2	0.0	0.0	0.0	0.6640415390928317	0.0
3	0.0	0.0	0.0	1.189395852403649	0.0
4	0.0	0.0	0.0	1.5845459423629304	0.0
5	0.0	0.0	0.0	2.7242274549414476	0.0
6	0.0	0.0	0.0	4.311732584198731	0.0
7	0.0	0.0	0.0	5.430305230264196	0.0
8	0.0	0.0	0.0	6.776735267194849	0.0
9	0.0	0.0	0.0	7.633123954420631	0.0
10	0.0	0.0	0.0	8.5623086392475	0.0
11	0.0	0.0	0.0	9.702581989204885	0.0
12	0.0	0.0	0.0	10.399371863261893	0.0
13	0.0	0.0	0.0	10.740467472617658	0.0
14	0.0	0.0	0.0	11.006991572235725	0.0
15	0.0	0.0	0.0	11.244121082036552	0.0
16	0.0	0.0	0.0	11.55069284429153	0.0
17	1.9727912629020547E-4	0.0	0.0	11.859237397809412	0.0
18	1.9727912629020547E-4	0.0	0.0	12.163441810548909	0.0
19	1.9727912629020547E-4	0.0	0.0	12.392877434424419	0.0
20	1.9727912629020547E-4	0.0	0.0	12.70181654619488	0.0
21	1.9727912629020547E-4	0.0	0.0	12.966367854550045	0.0
22	1.9727912629020547E-4	0.0	0.0	13.251830750291973	0.0
23	1.9727912629020547E-4	0.0	0.0	13.533150784381807	0.0
24	1.9727912629020547E-4	0.0	0.0	13.763967362141347	0.0
25	1.9727912629020547E-4	0.0	0.0	14.004253337962817	0.0
26	1.9727912629020547E-4	0.0	0.0	14.214750165714467	0.0
27	1.9727912629020547E-4	0.0	0.0	14.45819260755658	0.0
28	1.9727912629020547E-4	0.0	0.0	14.680920741138221	0.0
29	1.9727912629020547E-4	0.0	0.0	14.939553675704682	0.0
30	1.9727912629020547E-4	0.0	0.0	15.276111865155771	0.0
31	1.9727912629020547E-4	0.0	0.0	15.517186957482403	0.0
32	1.9727912629020547E-4	0.0	0.0	15.746425302231621	0.0
33	1.9727912629020547E-4	0.0	0.0	15.963037782898267	0.0
34	1.9727912629020547E-4	0.0	0.0	16.21437138979199	0.0
35	1.9727912629020547E-4	0.0	0.0	16.44597708405669	0.0
36	1.9727912629020547E-4	0.0	0.0	16.67718822006881	0.0
37	1.9727912629020547E-4	0.0	0.0	16.920433382784633	0.0
38	1.9727912629020547E-4	0.0	0.0	17.168413244531422	0.0
39	1.9727912629020547E-4	0.0	0.0	17.632611028692278	0.0
40	1.9727912629020547E-4	0.0	0.0	17.92320318171775	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGCGG	35	1.018052E-7	46.000004	2
TTGCGAG	70	0.0	46.000004	1
GTTTGCG	20	6.3081004E-4	46.0	1
GGTACGG	30	1.8593291E-6	46.0	2
GGCCGAT	20	6.3081004E-4	46.0	8
CCAGCGA	45	3.092282E-10	46.0	45
TCGGCCC	55	1.8189894E-12	46.0	8
CGTTATA	30	1.8593291E-6	46.0	11
ATCGTAG	25	3.414435E-5	46.0	1
GTAGCGG	45	3.092282E-10	46.0	2
AATTGCG	20	6.3081004E-4	46.0	1
CGCTAGG	30	1.8593291E-6	46.0	2
CGGGATA	85	0.0	46.0	6
GACGGGC	25	3.414435E-5	46.0	4
CGTACCC	25	3.414435E-5	46.0	37
CCGGTAT	20	6.3081004E-4	46.0	42
TGCGTAC	25	3.414435E-5	46.0	35
CGTTTTT	7305	0.0	44.835045	1
TTCGGAT	135	0.0	44.296295	22
TCGGATT	130	0.0	44.23077	23
>>END_MODULE