##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933085.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	858535
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.530792571065824	33.0	31.0	34.0	31.0	34.0
2	32.91251259412837	34.0	31.0	34.0	31.0	34.0
3	32.909621622880834	34.0	31.0	34.0	31.0	34.0
4	36.376223450412624	37.0	37.0	37.0	35.0	37.0
5	36.2541829977811	37.0	37.0	37.0	35.0	37.0
6	36.34637259983577	37.0	37.0	37.0	35.0	37.0
7	36.547899619701	37.0	37.0	37.0	35.0	37.0
8	36.50734448799408	37.0	37.0	37.0	35.0	37.0
9	38.33809338000198	39.0	39.0	39.0	37.0	39.0
10	38.04825312887652	39.0	39.0	39.0	35.0	39.0
11	37.73219961911862	39.0	38.0	39.0	35.0	39.0
12	37.349377719021355	39.0	37.0	39.0	35.0	39.0
13	37.24189928191629	39.0	37.0	39.0	35.0	39.0
14	38.05239390356829	41.0	37.0	41.0	33.0	41.0
15	38.354699575439554	41.0	37.0	41.0	33.0	41.0
16	38.58307815057045	41.0	37.0	41.0	34.0	41.0
17	38.622496461996306	41.0	37.0	41.0	35.0	41.0
18	38.499089728432736	40.0	37.0	41.0	35.0	41.0
19	38.38539954690257	40.0	37.0	41.0	35.0	41.0
20	38.16787084976151	40.0	35.0	41.0	34.0	41.0
21	38.12913393163936	40.0	35.0	41.0	34.0	41.0
22	38.07762642175334	40.0	35.0	41.0	34.0	41.0
23	38.05749561753452	40.0	35.0	41.0	34.0	41.0
24	38.00698864926881	40.0	35.0	41.0	34.0	41.0
25	37.865540717617804	40.0	35.0	41.0	34.0	41.0
26	37.76109884862003	40.0	35.0	41.0	34.0	41.0
27	37.651566913404814	40.0	35.0	41.0	34.0	41.0
28	37.62533851269896	40.0	35.0	41.0	34.0	41.0
29	37.54840862632275	40.0	35.0	41.0	34.0	41.0
30	37.463421992114476	40.0	35.0	41.0	34.0	41.0
31	37.28728706459259	40.0	35.0	41.0	33.0	41.0
32	37.07735269965698	40.0	35.0	41.0	33.0	41.0
33	36.86448426680333	40.0	35.0	41.0	33.0	41.0
34	36.57430390141345	39.0	35.0	41.0	31.0	41.0
35	36.41764750417863	39.0	35.0	41.0	31.0	41.0
36	36.30581746812885	39.0	35.0	41.0	31.0	41.0
37	36.15908961195525	39.0	35.0	41.0	30.0	41.0
38	36.11170307558807	39.0	35.0	41.0	30.0	41.0
39	36.05702504848375	39.0	35.0	41.0	30.0	41.0
40	35.98293604803531	39.0	35.0	41.0	30.0	41.0
41	35.844981276243836	39.0	35.0	41.0	29.0	41.0
42	35.87630090794202	39.0	35.0	41.0	30.0	41.0
43	35.82730465269325	39.0	35.0	41.0	29.0	41.0
44	35.781742153785224	38.0	35.0	41.0	30.0	41.0
45	35.72547187942251	38.0	35.0	41.0	29.0	41.0
46	35.68872789111684	38.0	35.0	41.0	29.0	41.0
47	35.60988660916561	38.0	35.0	41.0	29.0	41.0
48	35.50388860093066	38.0	35.0	41.0	28.0	41.0
49	35.474966075931675	38.0	35.0	41.0	28.0	41.0
50	35.38903015019772	37.0	35.0	41.0	28.0	41.0
51	35.31941039095669	37.0	35.0	40.0	28.0	41.0
52	35.091462782530705	37.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	5.0
14	9.0
15	18.0
16	40.0
17	115.0
18	212.0
19	414.0
20	671.0
21	1107.0
22	1815.0
23	3042.0
24	5256.0
25	8925.0
26	11728.0
27	12546.0
28	12580.0
29	12063.0
30	12413.0
31	14227.0
32	17336.0
33	24622.0
34	65237.0
35	87923.0
36	52540.0
37	59481.0
38	98971.0
39	353261.0
40	1977.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.26351866842936	21.36371842732096	24.39026947066806	17.982493433581624
2	40.692342187563696	22.220177395213938	24.280780632123328	12.806699785099035
3	31.914132795983857	22.515913736772525	32.44096047336451	13.128992993879107
4	28.730919531527544	25.520799967386303	31.977496549354424	13.77078395173173
5	24.581176073194452	29.804609014192785	31.035543105406305	14.578671807206462
6	26.922024145783226	35.52481844071587	28.55352431758752	8.999633095913387
7	84.06238534247294	1.5941108982161474	12.270553908693298	2.0729498506176216
8	82.41469479986256	1.7782618064493585	12.822074813490422	2.984968580197662
9	77.17693512786316	3.4776683536489488	14.70644761133792	4.638948907149971
10	46.33835545434956	22.065378813909742	20.07268195239565	11.523583779345046
11	31.24986168298322	22.636584414147357	30.679587902648116	15.433966000221307
12	29.812529483364102	18.191221091743493	33.983588321967076	18.012661102925332
13	28.52032823356066	18.682872567804456	36.806769671591724	15.990029527043163
14	18.8423302486212	20.66019440092716	40.963618256681436	19.5338570937702
15	16.100566662978213	26.3550117351069	40.01537502839139	17.5290465735235
16	28.187901483340806	23.909916310925006	32.241551014227724	15.66063119150646
17	25.862894349094677	22.281910463755118	30.90532127403076	20.94987391311944
18	22.646135568148065	22.72953344942256	35.8011030418096	18.823227940619777
19	22.405726033300912	24.63009661807614	31.435060888606753	21.52911646001619
20	18.22593138311193	34.09598909770714	30.03837933223456	17.63970018694637
21	23.281287309195314	24.846744745409328	36.216345285864875	15.65562265953048
22	20.15957415830455	21.327610406098763	38.58235249582137	19.930462939775314
23	20.64959494953613	23.02200842132237	37.01840926694893	19.309987362192572
24	21.460860652157454	26.736359030208433	32.63722504033033	19.165555277303778
25	23.42152620452282	24.73271328483987	32.63943811259879	19.20632239803852
26	19.14225977974107	27.337033434862878	29.565713686687207	23.954993098708847
27	22.40514364586185	24.005311373444297	34.00001164774878	19.589533332945074
28	17.963507603068017	27.144379670019276	30.40761296860349	24.484499758309212
29	21.409028170080425	27.953898210323402	29.637463819180347	20.999609800415826
30	25.77041122377073	23.32636409697916	29.93320016073893	20.970024518511185
31	23.25647760429103	23.66775961376065	33.60154216193865	19.474220620009667
32	25.994280955348355	23.66880791115097	29.901634761541462	20.435276371959212
33	26.884401917219446	22.897843419313133	31.529058221272283	18.688696442195134
34	20.62129091999744	23.96163231551422	36.49915262627616	18.917924138212186
35	23.389494895374096	27.86246338238977	30.18001595741583	18.5680257648203
36	28.167168490509997	24.76090083689075	27.65059083205693	19.42133984054232
37	22.601757645291105	26.85807800497359	30.89833262476195	19.641831724973354
38	25.192333451752113	23.535091755141025	32.12751955365827	19.145055239448595
39	23.804271229478122	25.577058594000246	28.44741332618938	22.171256850332252
40	28.346427344255037	23.25775885665698	30.555422900638877	17.8403908984491
41	22.415626619765064	23.92936805138987	28.809192403338248	24.84581292550682
42	21.716412260420366	25.87885176492513	32.01593412033289	20.388801854321606
43	21.132743568986704	25.505424938994913	32.92387613783946	20.43795535417892
44	25.443575392965922	24.317005130833337	28.837496432876936	21.4019230433238
45	21.260053463166905	23.51354341989552	28.677106932157688	26.549296184779887
46	23.45961434303785	23.67835906515168	30.048629351162155	22.813397240648314
47	20.0429801930032	23.718776753422983	33.39514405353305	22.843099000040766
48	20.987030231731964	27.973932338227332	29.824060754657644	21.214976675383067
49	24.39015299318024	24.753562755158498	30.39270385016336	20.4635804014979
50	22.098225465473163	24.658517125102648	30.181763119733034	23.061494289691158
51	21.550198885310444	25.24835912339043	29.04797125335601	24.153470737943124
52	21.786648185571934	25.483643648773786	30.32211849254835	22.407589673105928
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	373.0
1	1501.5
2	2630.0
3	13166.5
4	23703.0
5	14309.0
6	4915.0
7	5034.5
8	5154.0
9	5798.0
10	6442.0
11	7028.5
12	7615.0
13	7651.5
14	7423.0
15	7158.0
16	6671.0
17	6184.0
18	5837.0
19	5490.0
20	5348.5
21	5207.0
22	5111.0
23	5015.0
24	5473.5
25	5932.0
26	6380.0
27	6828.0
28	8141.0
29	9454.0
30	10463.0
31	11472.0
32	12225.5
33	12979.0
34	15370.0
35	17761.0
36	18776.5
37	19792.0
38	21498.0
39	24685.0
40	26166.0
41	28280.5
42	30395.0
43	35125.5
44	39856.0
45	44260.5
46	48665.0
47	56945.5
48	65226.0
49	72708.5
50	80191.0
51	78105.5
52	76020.0
53	67883.0
54	59746.0
55	55652.0
56	51558.0
57	48160.0
58	44762.0
59	46605.0
60	48448.0
61	37598.0
62	26748.0
63	22773.5
64	16573.0
65	14347.0
66	12280.5
67	10214.0
68	8992.0
69	7770.0
70	6574.0
71	5378.0
72	4413.5
73	3449.0
74	2838.5
75	2228.0
76	1983.5
77	1739.0
78	1308.0
79	877.0
80	633.5
81	390.0
82	326.5
83	263.0
84	227.0
85	191.0
86	140.0
87	89.0
88	53.0
89	9.0
90	1.0
91	3.0
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	858535.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.97895578916241
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.96480088962572	21.574295842459648
2	9.070765953037698	5.438641829599136
3	3.538108361493686	3.1820638243945516
4	2.0985394369007873	2.516480840026499
5	1.4523819193440684	2.1770446674497332
6	1.1720712369242152	2.1082483076099967
7	0.9927872148355847	2.083390681512117
8	0.8871662427160056	2.127705405441637
9	0.7613154584903918	2.054109822452043
>10	7.949874297706209	46.1983670750126
>50	0.07635084081226756	1.47611884508833
>100	0.024151795467676523	1.4703964931141966
>500	0.006622266499201597	1.3352802400339674
>1k	0.003895450881883292	1.9649663563767308
>5k	3.8954508818832924E-4	0.6052787724414951
>10k+	7.790901763766585E-4	3.6876109969872832
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18171	2.116512431059887	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	13406	1.5614972016283553	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	5183	0.6037028193375925	No Hit
GGGGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT	4432	0.5162282259896219	No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTT	1752	0.20406855864932705	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG	1641	0.1911395575020238	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	1587	0.18484977316009246	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	1466	0.17075599713465378	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	1410	0.1642332578170954	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT	1264	0.14722754459631815	No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCG	1199	0.13965650788843786	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC	1073	0.12498034442393147	No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1002	0.11671044278916992	No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCAGTTGTGGT	867	0.10098598193434163	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2102418655034448	0.0
2	0.0	0.0	0.0	0.5324185967957044	0.0
3	0.0	0.0	0.0	0.8926834666029924	0.0
4	0.0	0.0	0.0	1.2572580034593814	0.0
5	0.0	0.0	0.0	2.6565020645634716	0.0
6	0.0	0.0	0.0	3.7899444984770567	0.0
7	0.0	0.0	0.0	4.586999947585131	0.0
8	0.0	0.0	0.0	6.00231790200749	0.0
9	0.0	0.0	0.0	6.931924732247375	0.0
10	0.0	0.0	0.0	8.036713704158828	0.0
11	0.0	0.0	0.0	9.370031507160453	0.0
12	0.0	0.0	0.0	10.175473335391102	0.0
13	0.0	0.0	0.0	10.586172957421654	0.0
14	0.0	0.0	0.0	11.233438357201512	0.0
15	0.0	0.0	0.0	11.561438962884449	0.0
16	0.0	0.0	0.0	11.918558940520771	0.0
17	0.0	0.0	0.0	12.314232966623376	0.0
18	0.0	0.0	0.0	12.808330469928425	0.0
19	0.0	0.0	0.0	13.110240118341128	0.0
20	0.0	0.0	0.0	13.525132929932967	0.0
21	0.0	0.0	0.0	13.894599521277526	0.0
22	0.0	0.0	0.0	14.291205367282638	0.0
23	0.0	0.0	0.0	14.657876498919672	0.0
24	0.0	0.0	0.0	14.967590139015881	0.0
25	0.0	0.0	0.0	15.332514108335712	0.0
26	0.0	0.0	0.0	15.63919933374877	0.0
27	0.0	0.0	0.0	16.064575119243827	0.0
28	0.0	0.0	0.0	16.417152474855424	0.0
29	0.0	0.0	0.0	16.826920276983465	0.0
30	0.0	0.0	0.0	17.753498692540198	0.0
31	0.0	0.0	0.0	18.155462502984737	0.0
32	0.0	0.0	0.0	18.463079548300303	0.0
33	0.0	0.0	0.0	18.849784807841264	0.0
34	0.0	0.0	0.0	19.23858666216287	0.0
35	1.1647748781354283E-4	0.0	0.0	19.6172549750447	0.0
36	1.1647748781354283E-4	0.0	0.0	19.90204243274881	0.0
37	1.1647748781354283E-4	0.0	0.0	20.29037837711916	0.0
38	1.1647748781354283E-4	0.0	0.0	20.697583674515307	0.0
39	1.1647748781354283E-4	0.0	0.0	21.95449224551125	0.0
40	1.1647748781354283E-4	0.0	0.0	22.335606585637162	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGCA	20	6.310556E-4	46.000004	26
ATTAGCG	25	3.4164288E-5	46.0	1
GCGATTA	30	1.8608498E-6	46.0	9
CATACGA	175	0.0	44.68571	18
CGTTTTT	6200	0.0	44.14516	1
CGTAAGG	110	0.0	43.909092	2
TACGAAA	180	0.0	43.444443	20
GGGCGAT	620	0.0	42.661293	7
ATACGAA	185	0.0	42.270267	19
TACGAAT	60	1.8189894E-12	42.166668	12
GCAAGGC	1620	0.0	41.882713	46
CGACGCG	55	4.7293724E-11	41.81818	15
ACGCGCA	55	4.7293724E-11	41.81818	17
ATCGTAG	50	8.712959E-10	41.399998	1
AACGACG	50	8.712959E-10	41.399998	13
AGGGCGA	370	0.0	41.027023	6
AACACGT	440	0.0	40.772728	41
CACGACC	460	0.0	40.5	27
CGGGTAT	40	2.909419E-7	40.250004	6
GGCCGAT	40	2.909419E-7	40.250004	8
>>END_MODULE