FastQCFastQC Report
Sat 14 Jan 2017
SRR2933084.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933084.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739596
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109771.484188665163143No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC107451.4528201883190281No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG52400.7084949080308709No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCTT30620.41400981076155097Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT28300.382641333917436Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT26180.35397703611160686Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA14220.192267129622118No Hit
GGGGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT11580.15657196631674591No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCGTACTCGGTCG11460.15494945889377446No Hit
CGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC11380.15386778727846012No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCG11310.1529213246150601No Hit
TCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT10340.13980605627937415Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCGTACTCGGT8230.11127696742545931No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA8110.10965446000248784No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCA405.6061253E-946.00000420
GTTTGTC206.309987E-446.00000424
TATCACG206.309987E-446.00000414
CAGCGAC206.309987E-446.00000428
ACCGGTG206.309987E-446.00000442
CGAACCG206.309987E-446.00000426
TCCGCTT206.309987E-446.00000418
TCACCCG206.309987E-446.00000431
CGCGGTG206.309987E-446.00000416
CTCCGAC206.309987E-446.00000427
CAACGAA206.309987E-446.00000443
TCATTCG206.309987E-446.00000446
GGCAATT405.6061253E-946.0000048
ATCAGCG206.309987E-446.0000041
CCAATTG206.309987E-446.00000424
GATCCGT206.309987E-446.00000418
GGTCAAC206.309987E-446.0000049
TTCCGCT206.309987E-446.00000431
GTGGCTA206.309987E-446.00000411
TCGATTA206.309987E-446.00000411
Produced by FastQC (version 0.11.5)