##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933084.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	739596
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3820315415443	33.0	31.0	34.0	31.0	34.0
2	32.74820983347666	34.0	31.0	34.0	31.0	34.0
3	32.79233933120244	34.0	31.0	34.0	31.0	34.0
4	36.258925142915864	37.0	37.0	37.0	35.0	37.0
5	36.13013996830702	37.0	35.0	37.0	35.0	37.0
6	36.225935510738296	37.0	37.0	37.0	35.0	37.0
7	36.46698738230061	37.0	37.0	37.0	35.0	37.0
8	36.38820518228871	37.0	37.0	37.0	35.0	37.0
9	38.229071006333186	39.0	39.0	39.0	37.0	39.0
10	37.92754017057961	39.0	38.0	39.0	35.0	39.0
11	37.61983434199212	39.0	37.0	39.0	35.0	39.0
12	37.16951011092542	39.0	35.0	39.0	35.0	39.0
13	37.03255966771048	39.0	35.0	39.0	33.0	39.0
14	37.74647645471311	40.0	35.0	41.0	33.0	41.0
15	38.022821918993614	40.0	35.0	41.0	33.0	41.0
16	38.29339125684833	40.0	35.0	41.0	34.0	41.0
17	38.361066041460475	40.0	35.0	41.0	35.0	41.0
18	38.346740653005156	40.0	36.0	41.0	35.0	41.0
19	38.269168573112886	40.0	36.0	41.0	35.0	41.0
20	38.09365383263295	40.0	35.0	41.0	34.0	41.0
21	38.05657007339142	40.0	35.0	41.0	34.0	41.0
22	37.9949215517661	40.0	35.0	41.0	34.0	41.0
23	37.967426811394326	40.0	35.0	41.0	34.0	41.0
24	37.902629273278926	40.0	35.0	41.0	34.0	41.0
25	37.768777819241855	40.0	35.0	41.0	34.0	41.0
26	37.65620014169898	40.0	35.0	41.0	34.0	41.0
27	37.53731902281786	39.0	35.0	41.0	34.0	41.0
28	37.525853303695534	39.0	35.0	41.0	34.0	41.0
29	37.47291088648397	39.0	35.0	41.0	34.0	41.0
30	37.40891919372198	39.0	35.0	41.0	34.0	41.0
31	37.296344220358144	39.0	35.0	41.0	33.0	41.0
32	37.19852595200623	39.0	35.0	41.0	33.0	41.0
33	37.086928539364735	39.0	35.0	41.0	33.0	41.0
34	36.9447590306059	39.0	35.0	41.0	33.0	41.0
35	36.86845115441403	39.0	35.0	41.0	33.0	41.0
36	36.769256458931636	39.0	35.0	41.0	32.0	41.0
37	36.61673400072472	39.0	35.0	41.0	32.0	41.0
38	36.64283473680226	39.0	35.0	41.0	32.0	41.0
39	36.60844569197237	39.0	35.0	41.0	32.0	41.0
40	36.50016360283182	39.0	35.0	41.0	32.0	41.0
41	36.339999134662705	39.0	35.0	41.0	31.0	41.0
42	36.344658435145675	38.0	35.0	41.0	31.0	41.0
43	36.27163613648533	38.0	35.0	41.0	31.0	41.0
44	36.24426037999124	38.0	35.0	41.0	31.0	41.0
45	36.210765066333515	38.0	35.0	41.0	31.0	41.0
46	36.14681393625709	38.0	35.0	41.0	31.0	41.0
47	36.03622383030736	38.0	35.0	41.0	31.0	41.0
48	35.924718359753165	38.0	35.0	41.0	31.0	41.0
49	35.88281034510733	37.0	35.0	40.0	31.0	41.0
50	35.719774038799564	37.0	35.0	40.0	31.0	41.0
51	35.527232435005054	37.0	35.0	40.0	30.0	41.0
52	35.17963590933429	36.0	35.0	39.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	0.0
13	3.0
14	4.0
15	16.0
16	35.0
17	94.0
18	195.0
19	262.0
20	519.0
21	838.0
22	1406.0
23	2200.0
24	3287.0
25	4774.0
26	6248.0
27	7102.0
28	8071.0
29	9287.0
30	10698.0
31	13139.0
32	16863.0
33	22771.0
34	57571.0
35	96682.0
36	50776.0
37	52597.0
38	85169.0
39	287809.0
40	1178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.59295615444107	23.250936998036767	24.41860150676856	14.737505340753602
2	36.28278141039162	23.53014348373977	26.69159919739966	13.495475908468949
3	32.3678062077134	23.5900410494378	30.636996414258594	13.405156328590204
4	28.391446140866094	27.116020097458616	30.324528526384675	14.168005235290618
5	23.923195906954607	31.319125576666178	29.557488142174915	15.200190374204295
6	25.624800566795926	37.79306540327422	26.288135684887425	10.293998345042429
7	84.50032179730556	1.6530646461040892	10.44002401310986	3.4065895434804947
8	81.24597753368054	2.0877614265085263	10.939891508336984	5.72636953147394
9	76.32166750496216	3.313295366659636	14.185311981135648	6.179725147242548
10	49.101671723481466	16.91558634714087	19.783773844098672	14.19896808527899
11	32.503826413339176	23.01432133218676	28.243257129568033	16.23859512490603
12	31.959745590836082	17.946960232343063	31.41282538034278	18.680468796478078
13	28.000421851929975	22.956451900767448	33.78033412836197	15.26279211894061
14	16.2582004229336	26.26190514821605	37.87067534167302	19.609219087177323
15	12.715861091731162	30.24651296113013	39.82444469683449	17.21318125030422
16	22.646688191931812	26.123992017263483	35.39067274566114	15.838647045143563
17	20.635725450110602	26.054629824931446	28.808565757521677	24.50107896743628
18	18.65910578207562	25.73499586260607	35.74018788635958	19.865710468958728
19	19.89816061741816	28.07776137242495	29.247859642291196	22.7762183678657
20	17.15855683373085	36.090649489721415	27.556260444891535	19.1945332316562
21	21.237675704033013	29.007322916835676	33.1257875921449	16.62921378698641
22	17.696282835493975	25.664687207610644	33.70002001092489	22.939009945970504
23	18.225220255382666	27.2304068707781	32.06520857332922	22.479164300510007
24	19.55554113326735	29.79883612134192	30.510440835266824	20.135181910123904
25	20.93237389061055	26.677537466400576	30.664443831497195	21.72564481149168
26	17.243332846581104	30.885510467877058	27.189303349396155	24.68185333614568
27	21.829755704465683	27.776921454415653	29.779636450170095	20.613686390948573
28	16.29186745196026	30.953114943834205	27.720133694611654	25.034883909593887
29	20.35665417335951	29.1674103159022	27.012990876099924	23.46294463463837
30	24.391965343241445	26.280428774628312	27.599392100552194	21.728213781578052
31	21.644249022439276	27.956478942557826	29.538153262051175	20.86111877295172
32	27.155771529321417	25.25351678483929	24.350050568148017	23.240661117691282
33	25.304896186566722	24.992293089740887	29.111569018761596	20.5912417049308
34	19.1415313225058	25.87480191888545	31.274777040438295	23.70888971817046
35	22.393577033948265	27.34384718143419	26.461338352289626	23.80123743232792
36	26.143056479483395	24.816386243300396	27.65117712913537	21.389380148080843
37	20.22117480354139	29.380634833071028	27.984602404556004	22.41358795883158
38	23.61356740707089	25.685238968301615	27.87075646704417	22.83043715758333
39	22.20144511327806	25.44645995922098	27.840199243911538	24.51189568358942
40	26.53732578326546	23.42860156085214	28.917273754860762	21.11679890102164
41	22.8311132023429	23.971465502788007	26.140893136252764	27.056528158616327
42	21.8885715985484	25.794758219352186	30.489077820864363	21.82759236123505
43	21.079751648197124	25.413063348098152	32.15039021303522	21.3567947906695
44	22.593551073829495	25.391429915791864	27.821946035403112	24.19307297497553
45	19.205620365713173	24.73512566319991	26.28556671480105	29.773687256285864
46	23.6752226891438	24.05637672459018	28.75515822151553	23.513242364750486
47	19.04918360834834	25.404815602031377	31.612934629175925	23.933066160444348
48	19.44548104640912	27.19768630441484	29.67336221396546	23.683470435210573
49	24.61222072590982	23.111266150709305	29.901594924796782	22.374918198584094
50	21.884785747894796	23.22930356573048	29.76625076393058	25.119659922444143
51	19.716710203949184	24.38331197031893	27.19038502101147	28.709592804720412
52	19.642074862492496	25.212954099265005	31.74287043196556	23.40210060627694
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	600.0
1	1394.0
2	2188.0
3	7493.5
4	12799.0
5	7397.5
6	1996.0
7	2528.5
8	3061.0
9	4065.0
10	5069.0
11	6643.0
12	8217.0
13	8623.0
14	8632.0
15	8235.0
16	7011.5
17	5788.0
18	5179.5
19	4571.0
20	4254.5
21	3938.0
22	3826.0
23	3714.0
24	3519.5
25	3325.0
26	4085.0
27	4845.0
28	5368.0
29	5891.0
30	6424.0
31	6957.0
32	7830.0
33	8703.0
34	9833.5
35	10964.0
36	10777.0
37	10590.0
38	13147.0
39	17029.5
40	18355.0
41	24077.0
42	29799.0
43	32427.5
44	35056.0
45	39516.0
46	43976.0
47	52212.5
48	60449.0
49	74422.0
50	88395.0
51	87577.5
52	86760.0
53	76965.0
54	67170.0
55	57735.5
56	48301.0
57	43247.0
58	38193.0
59	37186.5
60	36180.0
61	27524.0
62	18868.0
63	15572.0
64	9974.5
65	7673.0
66	6284.0
67	4895.0
68	3755.5
69	2616.0
70	2238.0
71	1860.0
72	1476.5
73	1093.0
74	922.5
75	752.0
76	594.0
77	436.0
78	299.0
79	162.0
80	112.5
81	63.0
82	68.5
83	74.0
84	39.5
85	5.0
86	3.0
87	1.0
88	2.0
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	739596.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.964728780586693
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.44510825488472	14.349331309215533
2	12.16310836757638	5.099925361102466
3	5.511155200566878	3.466196221428134
4	3.0332799416741123	2.5436756517116628
5	1.8156484976573655	1.903228915713318
6	1.2027637457079106	1.512936942953333
7	0.900498102493022	1.3215088940339415
8	0.6104528187471827	1.0238382222703466
9	0.5003338977467895	0.9440428019395711
>10	3.8255232198739817	16.543291431600192
>50	1.0131984753207655	15.529966885666957
>100	0.9640580259752785	29.049792202739784
>500	0.0071124334578994385	0.951052211562238
>1k	0.005819263738281359	2.106385449752796
>5k	6.465848598090399E-4	0.7103069539033819
>10k+	0.0012931697196180798	2.9445205444063474
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10977	1.484188665163143	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	10745	1.4528201883190281	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	5240	0.7084949080308709	No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCTT	3062	0.41400981076155097	Illumina Single End Adapter 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	2830	0.382641333917436	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	2618	0.35397703611160686	Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	1422	0.192267129622118	No Hit
GGGGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT	1158	0.15657196631674591	No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCGTACTCGGTCG	1146	0.15494945889377446	No Hit
CGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC	1138	0.15386778727846012	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCG	1131	0.1529213246150601	No Hit
TCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT	1034	0.13980605627937415	Illumina Single End Adapter 2 (95% over 21bp)
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCGTACTCGGT	823	0.11127696742545931	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	811	0.10965446000248784	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4323982282218941	0.0
2	0.0	0.0	0.0	1.4279417411667992	0.0
3	0.0	0.0	0.0	2.12697202256367	0.0
4	0.0	0.0	0.0	2.952828300856143	0.0
5	0.0	0.0	0.0	5.239211677726759	0.0
6	0.0	0.0	0.0	7.231786002087627	0.0
7	0.0	0.0	0.0	8.877008528980687	0.0
8	0.0	0.0	0.0	11.842411262364859	0.0
9	0.0	0.0	0.0	13.362024672929545	0.0
10	0.0	0.0	0.0	15.005895110303463	0.0
11	0.0	0.0	0.0	17.560803465675857	0.0
12	0.0	0.0	0.0	18.900183343338796	0.0
13	0.0	0.0	0.0	19.435475583967463	0.0
14	0.0	0.0	0.0	19.650863444366923	0.0
15	0.0	0.0	0.0	19.984423928739474	0.0
16	0.0	0.0	0.0	20.678451478915516	0.0
17	0.0	0.0	0.0	21.429672415751302	0.0
18	0.0	0.0	0.0	22.20685347135463	0.0
19	0.0	0.0	0.0	22.664400564632583	0.0
20	0.0	0.0	0.0	23.20753492447228	0.0
21	0.0	0.0	0.0	23.74891156793709	0.0
22	0.0	0.0	0.0	24.25851410770204	0.0
23	0.0	0.0	0.0	24.734314409488423	0.0
24	0.0	0.0	0.0	25.08288308752346	0.0
25	1.3520895191428833E-4	0.0	0.0	25.456735839566466	0.0
26	1.3520895191428833E-4	0.0	0.0	25.791783622410072	0.0
27	1.3520895191428833E-4	0.0	0.0	26.215258059805624	0.0
28	1.3520895191428833E-4	0.0	0.0	26.559635260331316	0.0
29	1.3520895191428833E-4	0.0	0.0	26.979594264977095	0.0
30	1.3520895191428833E-4	0.0	0.0	28.01894547834223	0.0
31	1.3520895191428833E-4	0.0	0.0	28.414161244787696	0.0
32	1.3520895191428833E-4	0.0	0.0	28.702967566076616	0.0
33	1.3520895191428833E-4	0.0	0.0	29.043423706996794	0.0
34	1.3520895191428833E-4	0.0	0.0	29.421197518645315	0.0
35	1.3520895191428833E-4	0.0	0.0	29.80221634513978	0.0
36	1.3520895191428833E-4	0.0	0.0	30.0919691290921	0.0
37	1.3520895191428833E-4	0.0	0.0	30.462847284192993	0.0
38	1.3520895191428833E-4	0.0	0.0	30.882400661983027	0.0
39	1.3520895191428833E-4	0.0	0.0	32.20663713703157	0.0
40	1.3520895191428833E-4	0.0	0.0	32.57061963558483	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCA	40	5.6061253E-9	46.000004	20
GTTTGTC	20	6.309987E-4	46.000004	24
TATCACG	20	6.309987E-4	46.000004	14
CAGCGAC	20	6.309987E-4	46.000004	28
ACCGGTG	20	6.309987E-4	46.000004	42
CGAACCG	20	6.309987E-4	46.000004	26
TCCGCTT	20	6.309987E-4	46.000004	18
TCACCCG	20	6.309987E-4	46.000004	31
CGCGGTG	20	6.309987E-4	46.000004	16
CTCCGAC	20	6.309987E-4	46.000004	27
CAACGAA	20	6.309987E-4	46.000004	43
TCATTCG	20	6.309987E-4	46.000004	46
GGCAATT	40	5.6061253E-9	46.000004	8
ATCAGCG	20	6.309987E-4	46.000004	1
CCAATTG	20	6.309987E-4	46.000004	24
GATCCGT	20	6.309987E-4	46.000004	18
GGTCAAC	20	6.309987E-4	46.000004	9
TTCCGCT	20	6.309987E-4	46.000004	31
GTGGCTA	20	6.309987E-4	46.000004	11
TCGATTA	20	6.309987E-4	46.000004	11
>>END_MODULE