FastQCFastQC Report
Sat 14 Jan 2017
SRR2933083.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933083.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697029
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC147270.8678107445423737No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127870.7534933109569725No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG79830.4704103465527107No Hit
GCCCTGCTAGAGGACTTTCGTCTGTATCTATGCTCTGCCCCATGCAGCTCCT24270.1430146450060665No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGATT351.0199619E-746.00000442
TCGCGCG351.0199619E-746.0000041
TAGTACG700.046.0000041
GTCGCAA301.8619303E-646.023
GTCGATA253.417849E-546.036
CGAAACG253.417849E-546.043
CGACCGG253.417849E-546.033
CGATTTA253.417849E-546.012
CGACATA253.417849E-546.046
CCGTCGA253.417849E-546.043
ACTACGT206.31231E-446.018
TATAGCG501.6370905E-1146.01
CGCAATA206.31231E-446.031
CGCGTCA206.31231E-446.015
TTCGCTA301.8619303E-646.017
CGATAAC206.31231E-446.012
TCGAACG206.31231E-446.01
TCGCGAG453.110472E-1046.01
ATTCTCG301.8619303E-646.010
CCCGATA405.6134013E-946.040
Produced by FastQC (version 0.11.5)