FastQCFastQC Report
Sat 14 Jan 2017
SRR2933082.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933082.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277616
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203141.5899926112384315No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC113080.8850859726240122No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG59050.4621889519229565No Hit
GCCCTGCTAGAGGACTTTCGTCTGTATCTATGCTCTGCCCCATGCAGCTCCT28390.22221074250792103No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18130.1419049229189365No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA18050.14127875668432455No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14780.1156842118445605No Hit
GGGGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT14070.11012698651237932No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA13490.10558728131144256No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCTT13090.10245645013838274Illumina Single End Adapter 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG351.0196891E-746.0000041
CGTGCTA206.3117204E-446.032
ACGTTGT253.4173718E-546.019
TATTGCG206.3117204E-446.01
TACGCTT253.4173718E-546.023
GCGATCA405.6115823E-946.09
CGTAAGC206.3117204E-446.026
CGCGTTT301.8615683E-646.013
ATCGGAC206.3117204E-446.030
ATCGCGA253.4173718E-546.019
TCGAAAC206.3117204E-446.019
TACGTTA253.4173718E-546.033
CGTTTTT54950.043.6141971
TACGAAT2650.042.52830512
TACGGGA2250.041.911114
CGGGTAT554.7293724E-1141.8181846
ACGGGTA554.7293724E-1141.8181845
CATACGA8400.041.6190518
ACATACG8400.041.6190517
CGAATAT2750.040.9818214
Produced by FastQC (version 0.11.5)