##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933082.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1277616
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.506124688482295	33.0	31.0	34.0	31.0	34.0
2	32.90111504552228	34.0	31.0	34.0	31.0	34.0
3	32.944338517989756	34.0	31.0	34.0	31.0	34.0
4	36.394827553818985	37.0	37.0	37.0	35.0	37.0
5	36.314162471352894	37.0	37.0	37.0	35.0	37.0
6	36.395610261612255	37.0	37.0	37.0	35.0	37.0
7	36.52926622709797	37.0	37.0	37.0	35.0	37.0
8	36.48200711324843	37.0	37.0	37.0	35.0	37.0
9	38.311984195564236	39.0	39.0	39.0	37.0	39.0
10	38.017884873076106	39.0	39.0	39.0	35.0	39.0
11	37.69644243653805	39.0	37.0	39.0	35.0	39.0
12	37.220382337102855	39.0	35.0	39.0	35.0	39.0
13	37.07230732864961	39.0	35.0	39.0	34.0	39.0
14	37.94334291367672	40.0	36.0	41.0	33.0	41.0
15	38.19133370277141	40.0	36.0	41.0	33.0	41.0
16	38.41622991571803	40.0	36.0	41.0	34.0	41.0
17	38.45277610800115	40.0	36.0	41.0	35.0	41.0
18	38.34713560255977	40.0	36.0	41.0	35.0	41.0
19	38.2657817372356	40.0	36.0	41.0	35.0	41.0
20	38.08923729821793	40.0	35.0	41.0	34.0	41.0
21	38.03796837234349	40.0	35.0	41.0	34.0	41.0
22	38.00495767116254	40.0	35.0	41.0	34.0	41.0
23	37.95993944972511	40.0	35.0	41.0	34.0	41.0
24	37.91518108727505	40.0	35.0	41.0	34.0	41.0
25	37.79942877985248	40.0	35.0	41.0	34.0	41.0
26	37.68341113448798	40.0	35.0	41.0	34.0	41.0
27	37.58337481684637	40.0	35.0	41.0	34.0	41.0
28	37.54800894791549	40.0	35.0	41.0	34.0	41.0
29	37.49325384152985	40.0	35.0	41.0	34.0	41.0
30	37.43734032761017	40.0	35.0	41.0	34.0	41.0
31	37.29974734192433	40.0	35.0	41.0	33.0	41.0
32	37.15009830809883	40.0	35.0	41.0	33.0	41.0
33	36.997812331717824	39.0	35.0	41.0	33.0	41.0
34	36.78418241474747	39.0	35.0	41.0	32.0	41.0
35	36.659908767579616	39.0	35.0	41.0	32.0	41.0
36	36.56064419982217	39.0	35.0	41.0	32.0	41.0
37	36.42866009818287	39.0	35.0	41.0	31.0	41.0
38	36.40195723910784	39.0	35.0	41.0	31.0	41.0
39	36.34413548358818	39.0	35.0	41.0	31.0	41.0
40	36.24328671525716	39.0	35.0	41.0	31.0	41.0
41	36.10170035441009	38.0	35.0	41.0	31.0	41.0
42	36.106580537501095	38.0	35.0	41.0	31.0	41.0
43	36.05432070356038	38.0	35.0	41.0	31.0	41.0
44	36.0052300534746	38.0	35.0	41.0	31.0	41.0
45	35.944729089178594	38.0	35.0	41.0	31.0	41.0
46	35.86420880765426	38.0	35.0	41.0	31.0	41.0
47	35.775169534508024	37.0	35.0	41.0	31.0	41.0
48	35.67328367835093	37.0	35.0	41.0	30.0	41.0
49	35.61376892587444	37.0	35.0	40.0	30.0	41.0
50	35.49739436575622	37.0	35.0	40.0	30.0	41.0
51	35.41075800553531	36.0	35.0	40.0	29.0	41.0
52	35.163816044883596	36.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	3.0
14	10.0
15	35.0
16	78.0
17	169.0
18	337.0
19	583.0
20	1065.0
21	1708.0
22	2761.0
23	4345.0
24	6708.0
25	10383.0
26	13696.0
27	14879.0
28	15530.0
29	16059.0
30	17915.0
31	21209.0
32	26622.0
33	36695.0
34	102309.0
35	155695.0
36	77944.0
37	94396.0
38	153810.0
39	499500.0
40	3168.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.72284943206722	22.96143755244142	25.485435373382927	14.830277642108427
2	36.72848492817874	23.14733065334185	26.714991045822845	13.409193372656572
3	31.08453557250379	23.934108527131784	31.36732789821042	13.614028002154011
4	27.973585177392895	27.089829808017434	30.82898147800278	14.107603536586893
5	25.867396776496225	31.647850371316576	28.548875405442637	13.935877446744563
6	28.85632302663711	37.93205470188226	24.288988240598115	8.922634030882518
7	86.55041890521096	1.8385023356000552	9.580421660342388	2.0306570988466017
8	85.0507507733153	2.2758794504765123	10.129256364979774	2.544113411228413
9	79.5853370652841	4.274523800578577	12.214076843120312	3.9260622910170193
10	50.74779902568534	18.701941741493535	17.99805262301036	12.552206609810773
11	35.051533481108564	23.366567106235365	25.91772488760316	15.664174525052912
12	34.55975817460019	18.30150843445918	29.358977971471866	17.77975541946876
13	30.113899638075914	21.196666290966924	31.791477251380694	16.89795681957646
14	20.804686228099836	21.0042767153824	36.80182464840766	21.389212408110104
15	16.866178883169905	26.263525190667618	37.97815619090556	18.892139735256915
16	27.66832913801956	23.92252445179146	31.027397903595443	17.38174850659353
17	27.05343389563061	23.791107813302276	28.49878210667368	20.656676184393433
18	23.866952198469647	23.337685188663887	33.31799226058534	19.477370352281124
19	23.6316702358142	24.964386795406448	29.422690385843637	21.981252582935717
20	20.440414021114325	32.293819113098145	28.915260923469965	18.350505942317564
21	24.47668156942305	25.77104544714531	33.26649008778851	16.485782895643137
22	22.395618088690185	22.86547757698714	34.034091620643444	20.704812713679228
23	21.569313471340372	22.885827979612028	35.02453006224092	20.520328486806676
24	22.655868429950786	25.280992097782118	31.306120148777094	20.75701932349
25	24.34643899262376	24.362797585503	31.08046549197881	20.210297929894427
26	20.616679816157593	26.80954214724925	28.421685389037084	24.152092647556074
27	22.172781179947656	24.82154262313559	32.025819964684224	20.97985623223253
28	18.711412505792037	27.241048953676223	28.960423163141353	25.08711537739039
29	21.334735945698863	27.54395686966976	28.503321811874617	22.61798537275676
30	24.451634920038572	25.397615558978597	27.837707104482096	22.313042416500732
31	23.677145558602895	25.170004132697148	30.18755244142215	20.965297867277805
32	27.308831448572967	25.489348912349254	26.20630925097995	20.99551038809783
33	25.964687355199057	24.81637675170004	27.70128113611602	21.517654756984882
34	22.153917922129967	24.855825224480597	31.567074927051635	21.423181926337804
35	22.111495159735007	29.033371529473644	27.405339319482536	21.449793991308812
36	25.520187599403886	27.791370803120813	24.98614607205921	21.702295525416087
37	22.691559905323665	29.73005973625878	26.089764060562796	21.488616297854755
38	24.25752338730886	27.699011283515546	27.054686228099833	20.988779101075753
39	22.08777911359908	28.48539780340885	25.535059047475922	23.89176403551615
40	26.60494233008979	25.782081627030344	27.049129002767653	20.56384704011221
41	21.386942555509634	26.25695044520419	26.704502761393094	25.65160423789308
42	19.548205407571604	27.55178394760241	29.381128602021267	23.518882042804723
43	20.383432893764635	26.13563073724812	30.876883194950594	22.604053174036643
44	23.394196691337616	25.287253760128237	28.086216828843725	23.232332719690422
45	20.602356263540845	25.472285882456074	27.411366169490677	26.513991684512405
46	22.601705050656847	25.5692633780416	28.049742645677572	23.779288925623977
47	20.342105922280247	25.261737486067805	31.098232958885923	23.297923632766025
48	21.01852199721982	27.42091520456851	29.254486481070995	22.306076317140676
49	23.850750147149068	25.493027638977594	29.255504001202237	21.400718212671098
50	21.38576849381974	25.783881854954853	28.700955529674015	24.12939412155139
51	20.808521496286833	26.602907289827304	27.868310979198757	24.720260234687107
52	21.444001953638654	27.04224117418692	28.613292256828345	22.900464615346085
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1030.0
1	3817.5
2	6605.0
3	16744.5
4	26884.0
5	16137.5
6	5391.0
7	5741.5
8	6092.0
9	7130.5
10	8169.0
11	9225.5
12	10282.0
13	10777.0
14	10400.0
15	9528.0
16	9103.5
17	8679.0
18	8152.0
19	7625.0
20	7416.5
21	7208.0
22	7063.5
23	6919.0
24	7575.0
25	8231.0
26	9652.5
27	11074.0
28	11349.5
29	11625.0
30	13625.0
31	15625.0
32	17394.0
33	19163.0
34	23294.5
35	27426.0
36	27186.0
37	26946.0
38	30576.0
39	36545.5
40	38885.0
41	41703.0
42	44521.0
43	50507.5
44	56494.0
45	62998.0
46	69502.0
47	77692.5
48	85883.0
49	93641.0
50	101399.0
51	97879.5
52	94360.0
53	89376.5
54	84393.0
55	83643.0
56	82893.0
57	80810.0
58	78727.0
59	79159.0
60	79591.0
61	67837.5
62	56084.0
63	48716.5
64	35532.5
65	29716.0
66	25583.5
67	21451.0
68	17678.0
69	13905.0
70	11824.0
71	9743.0
72	8587.0
73	7431.0
74	6137.0
75	4843.0
76	3710.0
77	2577.0
78	2258.0
79	1939.0
80	1472.0
81	1005.0
82	751.0
83	497.0
84	391.0
85	285.0
86	182.5
87	80.0
88	71.0
89	37.0
90	12.0
91	9.0
92	6.0
93	3.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1277616.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.113173342891184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.78422459125642	16.316033483572674
2	9.542163516936093	4.2201503183244
3	3.616140054289789	2.3989299555804617
4	1.8926590271614367	1.674107885864346
5	1.1602420793038422	1.2828317109681182
6	0.8451784631445907	1.121374671671682
7	0.6480850651412501	1.0031852170485172
8	0.5078535100801428	0.8984202160958348
9	0.406184901144401	0.8083833415444133
>10	5.52398769205926	30.543613096399625
>50	1.8181627302765273	27.933562252583798
>100	0.24516946344336712	6.874744389963154
>500	0.005685089007381545	0.8674359749609537
>1k	0.0031978625666521194	1.1086523194473783
>5k	3.5531806296134656E-4	0.46396824566525646
>10k+	7.106361259226931E-4	2.484606920309355
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20314	1.5899926112384315	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	11308	0.8850859726240122	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	5905	0.4621889519229565	No Hit
GCCCTGCTAGAGGACTTTCGTCTGTATCTATGCTCTGCCCCATGCAGCTCCT	2839	0.22221074250792103	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1813	0.1419049229189365	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1805	0.14127875668432455	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1478	0.1156842118445605	No Hit
GGGGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT	1407	0.11012698651237932	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	1349	0.10558728131144256	No Hit
CTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTGCTT	1309	0.10245645013838274	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10590036442874855	0.0
2	0.0	0.0	0.0	0.29281098546042006	0.0
3	0.0	0.0	0.0	0.49279282663961627	0.0
4	0.0	0.0	0.0	0.7008365580894416	0.0
5	0.0	0.0	0.0	1.3631638927502474	0.0
6	0.0	0.0	0.0	2.042397715745576	0.0
7	0.0	0.0	0.0	2.6288023944596812	0.0
8	0.0	0.0	0.0	3.6367734906262914	0.0
9	0.0	0.0	0.0	4.229909456362475	0.0
10	0.0	0.0	0.0	4.90476011571552	0.0
11	0.0	0.0	0.0	5.793759627305858	0.0
12	7.82707793264956E-5	0.0	0.0	6.319817535159234	0.0
13	7.82707793264956E-5	0.0	0.0	6.5705188413419995	0.0
14	7.82707793264956E-5	0.0	0.0	6.816836983882481	0.0
15	7.82707793264956E-5	0.0	0.0	7.006017457514621	0.0
16	7.82707793264956E-5	0.0	0.0	7.287166096855393	0.0
17	7.82707793264956E-5	0.0	0.0	7.580211894653793	0.0
18	7.82707793264956E-5	0.0	0.0	7.905348711976055	0.0
19	7.82707793264956E-5	0.0	0.0	8.124037269414284	0.0
20	7.82707793264956E-5	0.0	0.0	8.444712652314937	0.0
21	7.82707793264956E-5	0.0	0.0	8.744881091032047	0.0
22	7.82707793264956E-5	0.0	0.0	9.074557613555246	0.0
23	7.82707793264956E-5	0.0	0.0	9.372847553568521	0.0
24	7.82707793264956E-5	0.0	0.0	9.602807103229765	0.0
25	7.82707793264956E-5	0.0	0.0	9.860865862669222	0.0
26	7.82707793264956E-5	0.0	0.0	10.083859312970407	0.0
27	7.82707793264956E-5	0.0	0.0	10.398194762745614	0.0
28	7.82707793264956E-5	0.0	0.0	10.631598226697223	0.0
29	1.565415586529912E-4	0.0	0.0	10.953134588170467	0.0
30	1.565415586529912E-4	0.0	0.0	11.677922004733817	0.0
31	2.348123379794868E-4	0.0	0.0	12.008694318167587	0.0
32	2.348123379794868E-4	0.0	0.0	12.272936469173835	0.0
33	3.130831173059824E-4	0.0	0.0	12.554163379293936	0.0
34	3.130831173059824E-4	0.0	0.0	12.883761631037808	0.0
35	3.130831173059824E-4	0.0	0.0	13.211324842519192	7.82707793264956E-5
36	3.130831173059824E-4	0.0	0.0	13.46108689935004	7.82707793264956E-5
37	4.696246759589736E-4	0.0	0.0	13.760942254949844	7.82707793264956E-5
38	4.696246759589736E-4	0.0	0.0	14.121300922969029	7.82707793264956E-5
39	5.478954552854692E-4	0.0	0.0	15.355865925285844	7.82707793264956E-5
40	5.478954552854692E-4	0.0	0.0	15.71356338680793	7.82707793264956E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAAG	35	1.0196891E-7	46.000004	1
CGTGCTA	20	6.3117204E-4	46.0	32
ACGTTGT	25	3.4173718E-5	46.0	19
TATTGCG	20	6.3117204E-4	46.0	1
TACGCTT	25	3.4173718E-5	46.0	23
GCGATCA	40	5.6115823E-9	46.0	9
CGTAAGC	20	6.3117204E-4	46.0	26
CGCGTTT	30	1.8615683E-6	46.0	13
ATCGGAC	20	6.3117204E-4	46.0	30
ATCGCGA	25	3.4173718E-5	46.0	19
TCGAAAC	20	6.3117204E-4	46.0	19
TACGTTA	25	3.4173718E-5	46.0	33
CGTTTTT	5495	0.0	43.614197	1
TACGAAT	265	0.0	42.528305	12
TACGGGA	225	0.0	41.91111	4
CGGGTAT	55	4.7293724E-11	41.818184	6
ACGGGTA	55	4.7293724E-11	41.818184	5
CATACGA	840	0.0	41.61905	18
ACATACG	840	0.0	41.61905	17
CGAATAT	275	0.0	40.98182	14
>>END_MODULE