##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933081.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1701223
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60069197277488	33.0	31.0	34.0	31.0	34.0
2	32.99043805544599	34.0	31.0	34.0	31.0	34.0
3	32.973211624813445	34.0	31.0	34.0	31.0	34.0
4	36.43857565998108	37.0	37.0	37.0	35.0	37.0
5	36.38243957435328	37.0	37.0	37.0	35.0	37.0
6	36.45762019441308	37.0	37.0	37.0	35.0	37.0
7	36.593368417897004	37.0	37.0	37.0	35.0	37.0
8	36.55257012161251	37.0	37.0	37.0	35.0	37.0
9	38.39143780680134	39.0	39.0	39.0	37.0	39.0
10	38.115073685225276	39.0	39.0	39.0	35.0	39.0
11	37.83988224941704	39.0	38.0	39.0	35.0	39.0
12	37.57750159738024	39.0	37.0	39.0	35.0	39.0
13	37.52147190579954	39.0	37.0	39.0	35.0	39.0
14	38.68099243896891	41.0	38.0	41.0	35.0	41.0
15	38.82751056151957	41.0	38.0	41.0	35.0	41.0
16	38.9703595589761	41.0	38.0	41.0	35.0	41.0
17	38.97258442896669	41.0	38.0	41.0	35.0	41.0
18	38.78518218951895	40.0	38.0	41.0	35.0	41.0
19	38.63587137018486	40.0	37.0	41.0	35.0	41.0
20	38.42097714408987	40.0	35.0	41.0	35.0	41.0
21	38.381871159748016	40.0	35.0	41.0	35.0	41.0
22	38.31585218398764	40.0	35.0	41.0	35.0	41.0
23	38.27386827006219	40.0	35.0	41.0	35.0	41.0
24	38.240740337980384	40.0	35.0	41.0	35.0	41.0
25	38.15540937313921	40.0	35.0	41.0	35.0	41.0
26	38.060395374386545	40.0	35.0	41.0	35.0	41.0
27	37.96854027955183	40.0	35.0	41.0	34.0	41.0
28	37.92349562638173	40.0	35.0	41.0	34.0	41.0
29	37.866128661557006	40.0	35.0	41.0	34.0	41.0
30	37.801225941572625	40.0	35.0	41.0	34.0	41.0
31	37.63354186958441	40.0	35.0	41.0	34.0	41.0
32	37.411421077660016	40.0	35.0	41.0	33.0	41.0
33	37.1375240047895	40.0	35.0	41.0	33.0	41.0
34	36.847588470177044	40.0	35.0	41.0	32.0	41.0
35	36.66941077095713	40.0	35.0	41.0	31.0	41.0
36	36.56228137051991	40.0	35.0	41.0	31.0	41.0
37	36.446917305961655	40.0	35.0	41.0	31.0	41.0
38	36.38844172692234	40.0	35.0	41.0	31.0	41.0
39	36.32934953265974	40.0	35.0	41.0	30.0	41.0
40	36.275123249568104	40.0	35.0	41.0	30.0	41.0
41	36.16227208308376	40.0	35.0	41.0	30.0	41.0
42	36.152470311064455	39.0	35.0	41.0	30.0	41.0
43	36.0971424675072	39.0	35.0	41.0	30.0	41.0
44	36.052133083082	39.0	35.0	41.0	30.0	41.0
45	36.00485826960957	39.0	35.0	41.0	30.0	41.0
46	35.953697428261904	39.0	35.0	41.0	29.0	41.0
47	35.88630473488779	39.0	35.0	41.0	29.0	41.0
48	35.77909715539938	39.0	35.0	41.0	29.0	41.0
49	35.72986962908449	39.0	35.0	41.0	29.0	41.0
50	35.63068098656085	38.0	35.0	41.0	28.0	41.0
51	35.54439071185847	38.0	35.0	41.0	28.0	41.0
52	35.311649325220735	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	4.0
13	8.0
14	12.0
15	34.0
16	68.0
17	166.0
18	328.0
19	593.0
20	1058.0
21	1766.0
22	2994.0
23	4956.0
24	9017.0
25	16447.0
26	23820.0
27	26109.0
28	25389.0
29	23050.0
30	22854.0
31	25266.0
32	30146.0
33	40584.0
34	102721.0
35	155333.0
36	106268.0
37	111907.0
38	191953.0
39	772961.0
40	5406.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.08859391155657	21.869560898247908	24.516891671462236	19.524953518733287
2	40.666038491132554	22.45660915705936	24.744786544738698	12.132565807069383
3	28.721690219330448	22.884007563970155	35.082055674065074	13.312246542634329
4	25.827125544387776	26.452616735137013	34.029224857646525	13.691032862828683
5	24.341782353048366	30.820239321946623	30.539029862634116	14.298948462370895
6	26.66599264176419	37.04323301530722	27.362785478446977	8.92798886448161
7	83.46906901681908	1.4770550362885995	13.182281217688685	1.871594729203638
8	82.4238209805534	1.8228062987627136	13.404944560472085	2.3484281602118005
9	77.07196528615002	3.865454440717061	15.43048736115136	3.632092911981557
10	40.69819183023037	28.84289713929332	19.79040960532511	10.6685014251512
11	26.2122014574221	21.57606615946293	36.39129026588519	15.82044211722978
12	27.238051683994396	18.175394995247537	35.53755151441052	19.04900180634755
13	26.891301140414864	19.224346249727404	37.19718108678286	16.68717152307487
14	18.73746122642358	20.469509288317873	39.491001473645724	21.302028011612823
15	15.891920106887811	27.036549588149235	39.82576064396026	17.2457696610027
16	25.295801902513666	26.141546405145004	33.81061742052629	14.752034271815043
17	24.177665126794075	24.5879581924298	33.23409100394245	18.000285676833666
18	20.21733776230394	24.296873484546115	37.03641439129379	18.449374361856147
19	19.717168178422227	26.264810668560205	34.08054088147174	19.937480271545823
20	18.011160206510258	32.01502683657581	32.95176470104155	17.022048255872395
21	22.608147197633702	26.21032045769426	35.93826323768254	15.243269106989501
22	20.732320219042418	22.98652204913759	37.62675439962898	18.65440333219102
23	19.510140645876525	23.48410525839352	37.964335069535274	19.041419026194685
24	19.445128592782957	25.09094927590328	36.101557526555894	19.36236460475787
25	21.59840303123106	25.854576384165977	34.249125482079656	18.297895102523302
26	19.692068588303826	27.92373486603461	31.47623797703182	20.907958568629745
27	20.067915846423425	25.35934442456985	35.135605385067095	19.437134343939626
28	17.595400485415492	27.28772183305775	33.29010952708728	21.82676815443948
29	19.448008873616214	26.059487792017862	33.5998866697664	20.892616664599526
30	21.98206819446951	24.087906171031076	33.88856134674878	20.041464287750635
31	20.319205653814933	24.373406660972723	35.92450842717268	19.382879258039658
32	22.285673306791644	24.45570039906585	33.29722205730818	19.961404236834323
33	21.58970340748979	24.55110235401238	33.59694760769164	20.262246630806192
34	19.376178196509215	24.765007291813006	35.38289806803693	20.47591644364084
35	19.89815562098561	27.18438441050938	32.42414427738162	20.49331569112339
36	21.031575519493916	27.75844201495042	30.36709473126098	20.84288773429468
37	20.161142895434637	28.867996729411722	30.88489868759122	20.085961687562417
38	20.967210060056797	27.57257572934295	31.824928301580684	19.63528590901957
39	19.90973552556014	28.54293646394388	29.984899099059913	21.562428911436065
40	23.266555883620196	26.520979318995803	31.115497497976456	19.096967299407545
41	19.61829813022749	26.94773113225015	30.626320006254325	22.807650731268033
42	19.041301463711694	28.18196085992254	32.11889329029763	20.65784438606814
43	19.704706555225272	27.21471553112085	32.40574574879366	20.67483216486022
44	21.25129980020256	26.421815364593588	31.038376509134896	21.288508326068953
45	19.260026463314922	25.949978339112512	30.932276368236263	23.857718829336306
46	21.161070594507596	25.730430402128352	31.213074358858307	21.895424644505745
47	19.034247714732285	25.532043712082427	33.08202393219466	22.35168464099063
48	19.268961212022177	27.248514744980522	32.0545278308605	21.427996212136797
49	21.130798255137627	25.955444994571554	31.758740623657218	21.155016126633605
50	19.784120012485136	26.10551350410851	32.276191892538485	21.834174590867864
51	19.18331694316383	26.078709257986755	31.990985308804316	22.7469884900451
52	19.744148768268474	26.5533677830596	31.747866093980626	21.9546173546913
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1150.0
1	3168.0
2	5186.0
3	36214.0
4	67242.0
5	39577.5
6	11913.0
7	11869.0
8	11825.0
9	12967.0
10	14109.0
11	15151.0
12	16193.0
13	16570.0
14	16338.5
15	15730.0
16	15192.0
17	14654.0
18	13761.0
19	12868.0
20	12677.0
21	12486.0
22	12666.0
23	12846.0
24	13702.5
25	14559.0
26	16651.5
27	18744.0
28	22119.0
29	25494.0
30	27393.5
31	29293.0
32	32955.0
33	36617.0
34	40152.5
35	43688.0
36	47736.0
37	51784.0
38	54719.5
39	60918.0
40	64181.0
41	71764.5
42	79348.0
43	88711.0
44	98074.0
45	108440.5
46	118807.0
47	121723.5
48	124640.0
49	129348.5
50	134057.0
51	120083.0
52	106109.0
53	101189.0
54	96269.0
55	89280.5
56	82292.0
57	77953.0
58	73614.0
59	75146.0
60	76678.0
61	63163.5
62	49649.0
63	42509.0
64	30000.5
65	24632.0
66	20599.0
67	16566.0
68	13950.5
69	11335.0
70	9142.0
71	6949.0
72	5861.5
73	4774.0
74	3851.5
75	2929.0
76	2427.5
77	1926.0
78	1486.0
79	1046.0
80	838.5
81	631.0
82	420.5
83	210.0
84	147.5
85	85.0
86	60.0
87	35.0
88	27.5
89	12.0
90	4.0
91	5.5
92	7.0
93	5.0
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1701223.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.678762973858056
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.85169583065502	18.19383903531277
2	8.812438408450857	4.525850342246651
3	3.8940663544013896	2.999844207574465
4	2.3449429328828524	2.4086093504288923
5	1.6056281037540625	2.061527175023287
6	1.2404992643455817	1.9112691947025287
7	0.9928045105739333	1.7845794194483628
8	0.8312269655993267	1.7075904181683497
9	0.6992125425490214	1.6159421833618464
>10	8.027494889668283	44.227637165655295
>50	0.6585026906238961	10.602502877420491
>100	0.03434243922650356	1.5686653173938163
>500	0.00299631853226284	0.5382963438683819
>1k	0.003457290614149431	1.747998264959735
>5k	2.304860409432954E-4	0.3315005851574279
>10k+	4.609720818865908E-4	3.774348119277689
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	52331	3.076081148679509	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	11440	0.6724574027038195	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	5601	0.32923373361399416	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTC	4085	0.24012137150743904	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTT	3393	0.1994447523928374	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT	3075	0.18075231759739904	No Hit
GGGGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCT	2884	0.16952510047183703	No Hit
CGCTGTCTCTTATACACATCTGACGCTTTCCGAATCGTATGCCGTCTTCTGC	2085	0.12255888851726082	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08993529948748635	0.0
2	0.0	0.0	0.0	0.24117943385435067	0.0
3	5.878124149508912E-5	0.0	0.0	0.4502643098523827	0.0
4	5.878124149508912E-5	0.0	0.0	0.6799226203736959	0.0
5	5.878124149508912E-5	0.0	0.0	1.4493102903029174	0.0
6	5.878124149508912E-5	0.0	0.0	2.5170127608197164	0.0
7	5.878124149508912E-5	0.0	0.0	3.312205395765282	0.0
8	5.878124149508912E-5	0.0	0.0	4.4282260467910435	0.0
9	5.878124149508912E-5	0.0	0.0	5.225534806430432	0.0
10	5.878124149508912E-5	0.0	0.0	5.995275163808625	0.0
11	5.878124149508912E-5	0.0	0.0	6.788292892818872	0.0
12	5.878124149508912E-5	0.0	0.0	7.267418792245343	0.0
13	1.1756248299017824E-4	0.0	0.0	7.548922157765325	0.0
14	1.1756248299017824E-4	0.0	0.0	7.948987287380902	0.0
15	1.1756248299017824E-4	0.0	0.0	8.15548578875315	0.0
16	1.1756248299017824E-4	0.0	0.0	8.355048103628977	0.0
17	1.1756248299017824E-4	0.0	0.0	8.591525038163722	0.0
18	1.1756248299017824E-4	0.0	0.0	8.855335249993681	0.0
19	1.1756248299017824E-4	0.0	0.0	9.058894689291174	0.0
20	1.1756248299017824E-4	0.0	0.0	9.326878369267286	0.0
21	1.7634372448526735E-4	0.0	0.0	9.574641302169086	0.0
22	1.7634372448526735E-4	0.0	0.0	9.849032137468162	0.0
23	1.7634372448526735E-4	0.0	0.0	10.115663848889886	0.0
24	2.351249659803565E-4	0.0	0.0	10.340208191401127	0.0
25	2.351249659803565E-4	0.0	0.0	10.573687282619622	0.0
26	2.351249659803565E-4	0.0	0.0	10.769546379281259	0.0
27	2.351249659803565E-4	0.0	0.0	11.012548031621957	0.0
28	2.351249659803565E-4	0.0	0.0	11.2322723123306	0.0
29	2.351249659803565E-4	0.0	0.0	11.498668898786343	0.0
30	2.351249659803565E-4	0.0	0.0	12.103821779978286	0.0
31	2.351249659803565E-4	0.0	0.0	12.425002483507454	0.0
32	2.351249659803565E-4	0.0	0.0	12.751473498771178	0.0
33	2.351249659803565E-4	0.0	0.0	13.027039958900156	0.0
34	2.351249659803565E-4	0.0	0.0	13.305075231171928	0.0
35	2.351249659803565E-4	0.0	0.0	13.594749189259726	0.0
36	2.351249659803565E-4	0.0	0.0	13.844687028096846	0.0
37	2.351249659803565E-4	0.0	0.0	14.127895049620186	0.0
38	2.351249659803565E-4	0.0	0.0	14.444843503761705	0.0
39	2.351249659803565E-4	0.0	0.0	15.246090606581266	0.0
40	2.351249659803565E-4	0.0	0.0	15.615648271860891	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15125	0.0	44.692234	1
AGGGATC	2680	0.0	41.794777	6
TAGTGCG	100	0.0	41.4	1
TATAGCG	45	1.589251E-8	40.88889	1
TATCGAG	80	0.0	40.250004	1
ACGGGAT	600	0.0	40.25	5
CGACCAA	385	0.0	40.02597	29
TACGGGA	460	0.0	40.0	4
TAGGGAT	2120	0.0	39.816036	5
TGCGACG	35	5.3746335E-6	39.428574	1
AATCGCG	35	5.3746335E-6	39.428574	1
GCGGGAT	920	0.0	39.25	5
CATACGA	900	0.0	39.1	18
TTGCGAG	190	0.0	38.736843	1
TAACGGG	375	0.0	38.64	3
ATAGGGA	1555	0.0	38.456593	4
TGCGGGA	815	0.0	38.380367	4
TCGATAC	30	1.00176825E-4	38.333332	25
CCGTATA	30	1.00176825E-4	38.333332	34
CGTAAGG	180	0.0	38.333332	2
>>END_MODULE