FastQCFastQC Report
Sat 14 Jan 2017
SRR2933080.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933080.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2016397
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240101.1907377366659442No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCTT31170.15458265411027688Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT27360.135687565494295Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC23850.11828027913154007No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT21850.1083615974433606Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21280.10553477316222945No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCG206.3125946E-446.018
ACGTTCG253.418079E-546.041
CGCCTCG206.3125946E-446.028
GTCGTGT206.3125946E-446.027
GCGATCG301.8621067E-646.08
CGCGTTA206.3125946E-446.035
CGGACCG253.418079E-546.05
CGTTTTT95050.045.0320851
CTCGCGG2350.045.021281
TATCCGG1400.044.3571431
AATCCGG1300.044.230771
CGACGGT3350.043.940327
ATTGCGG4750.043.578951
AGTACGG2000.042.5500031
GCGATGT2400.042.1666688
TCACGAC3550.042.11267524
CACGACG3500.042.05714425
CGGTCTA3450.041.99999630
CTTGCGG4550.041.9560431
CCAGCGG3550.041.464791
Produced by FastQC (version 0.11.5)