##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933080.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2016397
Sequences flagged as poor quality	0
Sequence length	52
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5287917012374	33.0	31.0	34.0	31.0	34.0
2	32.87305872801834	34.0	31.0	34.0	31.0	34.0
3	32.904922492941616	34.0	31.0	34.0	31.0	34.0
4	36.35834163609646	37.0	37.0	37.0	35.0	37.0
5	36.33502182357938	37.0	37.0	37.0	35.0	37.0
6	36.503089421378824	37.0	37.0	37.0	35.0	37.0
7	36.58635576228292	37.0	37.0	37.0	35.0	37.0
8	36.4288163491614	37.0	37.0	37.0	35.0	37.0
9	38.03811947746401	39.0	39.0	39.0	35.0	39.0
10	37.8891998946636	39.0	38.0	39.0	35.0	39.0
11	37.66433098244046	39.0	37.0	39.0	35.0	39.0
12	37.5880583039947	39.0	37.0	39.0	35.0	39.0
13	37.539142341513106	39.0	37.0	39.0	35.0	39.0
14	39.02372251099362	41.0	38.0	41.0	35.0	41.0
15	39.066444256760946	41.0	38.0	41.0	35.0	41.0
16	39.06441439855346	41.0	38.0	41.0	35.0	41.0
17	39.05344979188126	41.0	38.0	41.0	35.0	41.0
18	38.95841642295639	41.0	38.0	41.0	35.0	41.0
19	38.84894690876846	41.0	37.0	41.0	35.0	41.0
20	38.72331787837415	40.0	37.0	41.0	35.0	41.0
21	38.68541958751179	40.0	37.0	41.0	35.0	41.0
22	38.639031896992506	40.0	37.0	41.0	35.0	41.0
23	38.61119908430731	40.0	37.0	41.0	35.0	41.0
24	38.57140483744025	40.0	37.0	41.0	35.0	41.0
25	38.522350013415014	40.0	36.0	41.0	35.0	41.0
26	38.45386300416039	40.0	36.0	41.0	35.0	41.0
27	38.39418626391529	40.0	36.0	41.0	35.0	41.0
28	38.39954036829057	40.0	36.0	41.0	35.0	41.0
29	38.37092001227933	40.0	36.0	41.0	35.0	41.0
30	38.29664991566641	40.0	36.0	41.0	35.0	41.0
31	38.21481979987076	40.0	36.0	41.0	34.0	41.0
32	38.084873663271665	40.0	36.0	41.0	34.0	41.0
33	37.87878676669327	40.0	36.0	41.0	34.0	41.0
34	37.72477294897781	40.0	36.0	41.0	34.0	41.0
35	37.57490960361476	40.0	36.0	41.0	33.0	41.0
36	37.46454046499772	40.0	35.0	41.0	33.0	41.0
37	37.37977094788378	40.0	35.0	41.0	33.0	41.0
38	37.32147935153643	40.0	35.0	41.0	33.0	41.0
39	37.222158136517756	40.0	35.0	41.0	33.0	41.0
40	37.19999831382411	40.0	35.0	41.0	33.0	41.0
41	37.17793420640876	40.0	35.0	41.0	33.0	41.0
42	37.14770256055727	40.0	35.0	41.0	33.0	41.0
43	37.08006359858698	40.0	35.0	41.0	33.0	41.0
44	37.01895212103569	40.0	35.0	41.0	32.0	41.0
45	36.9694092978714	40.0	35.0	41.0	32.0	41.0
46	36.89781278190753	40.0	35.0	41.0	32.0	41.0
47	36.803107225412454	39.0	35.0	41.0	32.0	41.0
48	36.72277433461764	39.0	35.0	41.0	32.0	41.0
49	36.63839313389179	39.0	35.0	41.0	31.0	41.0
50	36.52167256745572	39.0	35.0	41.0	31.0	41.0
51	36.411348558840345	39.0	35.0	41.0	31.0	41.0
52	36.18286527901004	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	4.0
14	7.0
15	19.0
16	32.0
17	128.0
18	273.0
19	496.0
20	844.0
21	1445.0
22	2348.0
23	4043.0
24	7513.0
25	13177.0
26	18990.0
27	20640.0
28	20597.0
29	20756.0
30	22787.0
31	27068.0
32	33572.0
33	44587.0
34	92833.0
35	182237.0
36	109398.0
37	138775.0
38	244570.0
39	1001879.0
40	7376.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.30499599037293	22.339450019019072	26.495476833183147	19.86007715742485
2	28.244437975259835	23.961204068444854	26.40675422548238	21.38760373081293
3	24.55384529931358	24.73739050395334	29.39128554545558	21.3174786512775
4	23.840543305708152	28.72534525691121	26.700743950720025	20.73336748666061
5	22.408583230385684	36.29533271473822	23.63373879250961	17.66234526236649
6	87.8297775686038	2.091106066910435	8.170910787905358	1.9082055765804056
7	89.60998255799825	1.344328522607403	6.751844998777523	2.293843920616823
8	82.1670038191884	4.656573085558052	9.852524081319304	3.3238990139342603
9	39.09626923666322	36.57236149428907	13.93287135420257	10.398497914845143
10	24.935169016815635	22.96769931714836	35.94282276754032	16.154308898495685
11	26.103887280133826	22.1232723516252	32.639157864249945	19.13368250399103
12	20.55636861193505	27.86008905984288	33.638762604784674	17.9447797234374
13	16.8692970679881	30.313673349047832	33.87552153668152	18.94150804628255
14	16.564991913794756	27.73625431896596	37.72357328442762	17.975180482811666
15	19.78816671518555	25.258865193709372	36.73056446721553	18.22240362388954
16	20.05835160437156	25.74800498116194	31.780100843236724	22.413542571229772
17	20.345001505159946	25.40159502320228	34.14962430513436	20.103779166503422
18	22.577250412493175	27.903483292228664	31.308765089414436	18.21050120586373
19	22.221169739887532	27.782078628365348	30.63141831692866	19.36533331481846
20	19.84514954148414	28.766309412283398	32.05936132616742	19.329179720065046
21	19.575113432523455	25.749492783415175	32.41445013060424	22.260943653457133
22	16.63982836713207	28.498951347378515	31.675161190975786	23.18605909451363
23	16.200232394711954	27.96284660213242	36.213404403993856	19.62351659916177
24	18.319209957166173	28.727081026206648	32.758132451099655	20.195576565527524
25	17.935704129692713	30.621995569324888	30.469942179045102	20.972358121937297
26	17.048130898825974	28.53788217300462	32.47039149532557	21.94359543284383
27	18.240455624562028	27.266307180580014	33.880480877525606	20.61275631733235
28	17.769962958683237	26.453421622825267	32.628048940759186	23.148566477732313
29	20.44250214615475	27.73273318696665	31.42511122561678	20.399653441261815
30	21.90163940930283	27.201984529832167	29.27563371697141	21.62074234389359
31	23.138151861959724	27.201686969381527	29.607364026032574	20.05279714262618
32	23.257523196076964	27.42084024128185	28.514870831488043	20.80676573115314
33	19.5808662679026	27.378437877064883	30.656363801374432	22.384332053658085
34	20.794317785634476	26.955802850331555	30.846653709562155	21.403225654471814
35	24.299579894237098	27.162855330572306	28.98804154142265	19.549523233767953
36	20.713083782608287	29.496076417491196	30.296166875868195	19.494672924032322
37	21.86399801229619	28.573837394124276	30.480208014592364	19.081956578987175
38	21.129817193737146	28.298197230009762	29.74553126194891	20.826454314304176
39	23.348179946706924	26.790260052955837	30.15110615617857	19.710453844158664
40	19.801705715689916	25.956346890022154	31.67114412489207	22.57080326939586
41	20.977218275964503	27.258074674778825	29.257085782214514	22.507621267042154
42	20.848523381060378	25.932095713294558	31.05301188208473	22.16636902356034
43	21.275175473877418	27.954614096331227	30.346008251351297	20.42420217844006
44	19.381153612111106	30.348438328364903	27.986651438184047	22.283756621339947
45	20.44691595950599	28.532426898076125	29.61599327910129	21.404663863316596
46	21.94508323509706	26.776026744733304	29.804001890500732	21.47488812966891
47	20.706587046102527	26.388702224809897	31.326420342819393	21.578290386268183
48	20.587562865844376	25.12481421069363	30.951990109090623	23.335632814371376
49	20.39608271585407	27.601211467781393	30.202633707548664	21.800072108815876
50	19.87316981725325	27.25787630114506	30.515022587317876	22.353931294283814
51	19.90178521392365	25.785596784760145	31.195692118169188	23.11692588314702
52	20.638644076538498	25.1641913769957	32.24484067373638	21.95232387272943
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2363.0
1	2163.5
2	1964.0
3	17172.5
4	32381.0
5	19956.5
6	7532.0
7	7915.0
8	8298.0
9	9094.5
10	9891.0
11	10553.0
12	11215.0
13	11391.5
14	11268.0
15	10968.0
16	10180.5
17	9393.0
18	9395.0
19	9397.0
20	9812.0
21	10227.0
22	10985.5
23	11744.0
24	12979.0
25	14214.0
26	17105.5
27	19997.0
28	23943.5
29	27890.0
30	31844.0
31	35798.0
32	41897.5
33	47997.0
34	54599.0
35	61201.0
36	67450.5
37	73700.0
38	79011.0
39	89129.0
40	93936.0
41	103588.5
42	113241.0
43	127940.0
44	142639.0
45	148739.5
46	154840.0
47	162951.0
48	171062.0
49	181566.5
50	192071.0
51	191770.5
52	191470.0
53	172082.0
54	152694.0
55	128233.5
56	103773.0
57	87918.0
58	72063.0
59	60313.5
60	48564.0
61	39848.0
62	31132.0
63	25161.5
64	15766.5
65	12342.0
66	9698.0
67	7054.0
68	5426.0
69	3798.0
70	2790.0
71	1782.0
72	1499.0
73	1216.0
74	982.0
75	748.0
76	597.0
77	446.0
78	294.5
79	143.0
80	108.0
81	73.0
82	58.5
83	44.0
84	23.5
85	3.0
86	4.0
87	5.0
88	3.0
89	1.5
90	2.0
91	2.5
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2016397.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.062611455642376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.37936048658669	19.587745102563698
2	11.071132058730837	5.992274905590774
3	4.144372086930749	3.364725945486498
4	2.141998883585463	2.3187233409957257
5	1.3457427338730816	1.8209656363030575
6	0.9049838608211936	1.469473595941866
7	0.695041280987281	1.3166742493093524
8	0.544394811621353	1.178619621230104
9	0.45308416788584344	1.1035476712977859
>10	5.062890786602275	30.944680576170423
>50	1.0271558030666543	19.218011471809458
>100	0.2235662474164827	8.619768699496486
>500	0.0036922305248236155	0.6460418123333865
>1k	0.00239994984113535	1.2191909632953084
>5k	0.0	0.0
>10k+	1.8461152624118078E-4	1.1995564081760584
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24010	1.1907377366659442	No Hit
CTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCTT	3117	0.15458265411027688	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	2736	0.135687565494295	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGC	2385	0.11828027913154007	No Hit
GCTGTCTCTTATACACATCTGACGCGCTGAGGTTCGTATGCCGTCTTCTGCT	2185	0.1083615974433606	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2128	0.10553477316222945	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.9593408440897306E-5	0.0	0.0	0.1600379290387756	0.0
2	4.9593408440897306E-5	0.0	0.0	0.4938015678460145	0.0
3	4.9593408440897306E-5	0.0	0.0	0.9669226843721748	0.0
4	4.9593408440897306E-5	0.0	0.0	1.2839237511263903	0.0
5	4.9593408440897306E-5	0.0	0.0	2.8194844566818933	0.0
6	4.9593408440897306E-5	0.0	0.0	3.353307905139712	0.0
7	9.918681688179461E-5	0.0	0.0	4.542805806594634	0.0
8	9.918681688179461E-5	0.0	0.0	6.043651126241509	0.0
9	9.918681688179461E-5	0.0	0.0	7.3416098119566735	0.0
10	9.918681688179461E-5	0.0	0.0	8.21524729505152	0.0
11	9.918681688179461E-5	0.0	0.0	9.096918910313793	0.0
12	1.4878022532269192E-4	0.0	0.0	9.72467227435867	0.0
13	1.4878022532269192E-4	0.0	0.0	10.040631879535628	0.0
14	1.4878022532269192E-4	0.0	0.0	10.245651030030297	0.0
15	1.4878022532269192E-4	0.0	0.0	10.7675720604623	0.0
16	1.4878022532269192E-4	0.0	0.0	11.543907276196107	0.0
17	1.4878022532269192E-4	0.0	0.0	12.345287163192566	0.0
18	1.4878022532269192E-4	0.0	0.0	12.873804116947209	0.0
19	1.4878022532269192E-4	0.0	0.0	13.373110553130163	0.0
20	1.4878022532269192E-4	0.0	0.0	13.955287574817856	0.0
21	2.9756045064538385E-4	0.0	0.0	14.62147583040443	0.0
22	3.471538590862811E-4	0.0	0.0	15.310477053873816	0.0
23	3.471538590862811E-4	0.0	0.0	15.837010271290822	0.0
24	3.471538590862811E-4	0.0	0.0	16.256223352841726	0.0
25	3.471538590862811E-4	0.0	0.0	16.67533724757575	0.0
26	3.471538590862811E-4	0.0	0.0	17.114338099094574	0.0
27	3.471538590862811E-4	0.0	0.0	17.559587720076948	0.0
28	3.471538590862811E-4	0.0	0.0	18.044065727136076	0.0
29	3.471538590862811E-4	0.0	0.0	18.60104929733579	0.0
30	3.471538590862811E-4	0.0	0.0	19.09554517289998	0.0
31	3.471538590862811E-4	0.0	0.0	19.528247661546807	0.0
32	3.471538590862811E-4	0.0	0.0	19.98306881035828	0.0
33	3.471538590862811E-4	0.0	0.0	20.467497224008962	0.0
34	3.471538590862811E-4	0.0	0.0	20.997799540467476	0.0
35	3.471538590862811E-4	0.0	0.0	21.479847470513	0.0
36	3.471538590862811E-4	0.0	0.0	21.953464521123568	0.0
37	3.471538590862811E-4	0.0	0.0	22.456788023390235	0.0
38	3.471538590862811E-4	0.0	0.0	23.13750714764999	0.0
39	3.471538590862811E-4	0.0	0.0	23.64693063915489	0.0
40	7.439011266134596E-4	0.0	0.0	24.11137290920389	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTCG	20	6.3125946E-4	46.0	18
ACGTTCG	25	3.418079E-5	46.0	41
CGCCTCG	20	6.3125946E-4	46.0	28
GTCGTGT	20	6.3125946E-4	46.0	27
GCGATCG	30	1.8621067E-6	46.0	8
CGCGTTA	20	6.3125946E-4	46.0	35
CGGACCG	25	3.418079E-5	46.0	5
CGTTTTT	9505	0.0	45.032085	1
CTCGCGG	235	0.0	45.02128	1
TATCCGG	140	0.0	44.357143	1
AATCCGG	130	0.0	44.23077	1
CGACGGT	335	0.0	43.9403	27
ATTGCGG	475	0.0	43.57895	1
AGTACGG	200	0.0	42.550003	1
GCGATGT	240	0.0	42.166668	8
TCACGAC	355	0.0	42.112675	24
CACGACG	350	0.0	42.057144	25
CGGTCTA	345	0.0	41.999996	30
CTTGCGG	455	0.0	41.956043	1
CCAGCGG	355	0.0	41.46479	1
>>END_MODULE