FastQCFastQC Report
Sat 14 Jan 2017
SRR2933079.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933079.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153198
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC143551.2447992452293535No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC99890.8661999066942536No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97520.8456483622066635No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC96900.840272008796408No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC43030.3731362697472594No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC41530.36012896310954406No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCTT28770.24948014131137933Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT22750.1972774840053486No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT21860.1895598154003042No Hit
GGGTAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC17740.15383307983537953No Hit
ACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC13700.118800067291133No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCG13380.11602517520842041No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA12890.11177612170676675No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC11970.10379830696896804No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAC206.3114637E-446.014
CGTGGAT206.3114637E-446.040
CGGCCTA206.3114637E-446.05
CGCATCG206.3114637E-446.020
GGCCGTA206.3114637E-446.016
TTCACGT206.3114637E-446.09
TCGGGTA453.092282E-1046.04
CCGCTTG206.3114637E-446.038
TGCGGAT501.6370905E-1146.026
ACCTCGC206.3114637E-446.018
GCCGATT253.4171626E-546.08
CTAACGG1500.046.01
ACGATAC206.3114637E-446.046
CTCGGTA206.3114637E-446.031
AACGCGA206.3114637E-446.017
AGCGCCG206.3114637E-446.017
AGCGCAT206.3114637E-446.032
GATATCG206.3114637E-446.014
GAACGTT206.3114637E-446.08
CGAATGC453.092282E-1046.045
Produced by FastQC (version 0.11.5)