##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933079.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1153198
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.522265907502444	33.0	31.0	34.0	31.0	34.0
2	32.85203408261201	34.0	31.0	34.0	31.0	34.0
3	32.921245961231286	34.0	31.0	34.0	31.0	34.0
4	36.343356474777096	37.0	37.0	37.0	35.0	37.0
5	36.31844748256587	37.0	37.0	37.0	35.0	37.0
6	36.474999956642314	37.0	37.0	37.0	35.0	37.0
7	36.547933659267535	37.0	37.0	37.0	35.0	37.0
8	36.39578545921862	37.0	37.0	37.0	35.0	37.0
9	38.02255206824847	39.0	39.0	39.0	35.0	39.0
10	37.91595805750617	39.0	38.0	39.0	35.0	39.0
11	37.39827072194021	39.0	35.0	39.0	35.0	39.0
12	37.17726444201256	39.0	35.0	39.0	34.0	39.0
13	36.91312940188936	39.0	35.0	39.0	33.0	39.0
14	38.410860927611736	40.0	36.0	41.0	33.0	41.0
15	38.55654883203058	40.0	36.0	41.0	35.0	41.0
16	38.60690098317895	40.0	36.0	41.0	35.0	41.0
17	38.634218061425706	40.0	36.0	41.0	35.0	41.0
18	38.568924850719476	40.0	36.0	41.0	35.0	41.0
19	38.4418686123285	40.0	36.0	41.0	35.0	41.0
20	38.344480306070594	40.0	35.0	41.0	35.0	41.0
21	38.34837642798548	40.0	35.0	41.0	35.0	41.0
22	38.32712769186211	40.0	35.0	41.0	35.0	41.0
23	38.26657607800222	40.0	35.0	41.0	35.0	41.0
24	38.21328427555372	40.0	35.0	41.0	35.0	41.0
25	38.15915133394265	40.0	35.0	41.0	35.0	41.0
26	38.07590196999995	40.0	35.0	41.0	34.0	41.0
27	38.04601724942291	40.0	35.0	41.0	34.0	41.0
28	38.022141904512495	40.0	35.0	41.0	34.0	41.0
29	37.98423601150886	40.0	35.0	41.0	34.0	41.0
30	37.85128659605723	40.0	35.0	41.0	34.0	41.0
31	37.79667151694679	40.0	35.0	41.0	34.0	41.0
32	37.719989975702354	40.0	35.0	41.0	34.0	41.0
33	37.557853031309456	40.0	35.0	41.0	34.0	41.0
34	37.477374223680584	40.0	35.0	41.0	34.0	41.0
35	37.352791975012096	40.0	35.0	41.0	33.0	41.0
36	37.29085638372595	40.0	35.0	41.0	33.0	41.0
37	37.19654127044966	40.0	35.0	41.0	33.0	41.0
38	37.229859052825276	40.0	35.0	41.0	33.0	41.0
39	37.14458315050841	40.0	35.0	41.0	33.0	41.0
40	37.09651681671318	39.0	35.0	41.0	33.0	41.0
41	37.101247140560424	39.0	35.0	41.0	33.0	41.0
42	37.08529844831504	39.0	35.0	41.0	33.0	41.0
43	37.00895596419696	39.0	35.0	41.0	33.0	41.0
44	36.91541001631983	39.0	35.0	41.0	33.0	41.0
45	36.87041947696753	39.0	35.0	41.0	33.0	41.0
46	36.792461485365045	39.0	35.0	41.0	33.0	41.0
47	36.681770173031865	38.0	35.0	41.0	33.0	41.0
48	36.659299617238325	38.0	35.0	41.0	33.0	41.0
49	36.57341063720194	38.0	35.0	41.0	33.0	41.0
50	36.49377123442809	38.0	35.0	41.0	32.0	41.0
51	36.39361323901013	38.0	35.0	41.0	32.0	41.0
52	36.14064627236606	37.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	1.0
13	7.0
14	7.0
15	22.0
16	31.0
17	68.0
18	166.0
19	290.0
20	607.0
21	1012.0
22	1572.0
23	2345.0
24	3741.0
25	5897.0
26	7807.0
27	8648.0
28	9223.0
29	10307.0
30	12547.0
31	15971.0
32	20864.0
33	29598.0
34	78618.0
35	152503.0
36	72829.0
37	83363.0
38	132975.0
39	493489.0
40	8686.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.552609352426906	22.48234908489262	25.266346282251618	17.698695280428858
2	28.54843660845752	24.89997381195597	26.503948151141433	20.047641428445072
3	29.207473478101765	24.199226845693453	26.076441339648525	20.516858336556254
4	23.771459888067792	26.748919092818408	24.942464346972507	24.537156672141297
5	21.14589168555617	39.13144143503544	21.108950934705057	18.613715944703337
6	87.33409180383595	2.911989094674115	7.706135459825633	2.047783641664311
7	89.8262917556222	1.7523443502330043	5.482493032419411	2.9388708617253934
8	82.40258828059015	3.713325898934962	9.359277418101662	4.5248084023732265
9	40.849793357255216	29.25195846680275	14.45658074328953	15.441667432652503
10	35.656582824458596	21.73442895322399	23.355486221793655	19.253502000523763
11	32.808242816931696	20.764517454938353	29.01574577826184	17.411493949868106
12	23.078517305787905	29.31647470772582	30.82670972374215	16.778298262744126
13	16.836484281103505	36.8936644010829	30.433889063283146	15.835962254530445
14	14.916172244488804	30.489126758804648	38.00075962670764	16.59394136999891
15	17.936121984255955	20.854528016871342	41.939025215097494	19.270324783775205
16	18.92094852748617	23.61493863152728	25.720041137775123	31.744071703211418
17	21.687429218573044	29.18657507210384	28.973428674000473	20.15256703532264
18	24.52267520408464	33.21727925299905	24.609130435536656	17.650915107379653
19	21.881845095118095	28.04323281864866	24.107395260831186	25.967526825402054
20	22.055796142553145	28.771295128850376	29.294795863329625	19.87811286526685
21	17.536884385855682	24.597337144185126	28.623618840823518	29.24215962913567
22	13.972188644101013	33.55382163340554	27.03161122374475	25.442378498748695
23	15.464820438467635	33.778761322860426	32.0095074739984	18.746910764673544
24	24.32947334282578	26.848121484775383	27.523460845405562	21.298944326993283
25	15.477914460482936	31.578011755136586	24.46318845506149	28.48088532931899
26	14.522657861009126	30.8195990627802	31.455656357364475	23.2020867188462
27	17.31723433443346	28.48834285179128	33.36530240253625	20.829120411239007
28	15.628539071347678	34.04870629328181	26.749959677349423	23.572794958021085
29	20.068019542177492	30.120499688691794	30.56500271419132	19.246478054939395
30	27.849077088236367	30.12873764956235	23.93656596698919	18.085619295212098
31	27.00594347197966	31.641834273038977	22.078862432990693	19.273359821990674
32	24.9481875618931	30.50265435770787	20.148231266443403	24.400926813955625
33	18.234856460035484	29.5950045005281	25.646072920695318	26.524066118741104
34	20.839439541171593	28.912641194313554	25.139828546355442	25.108090718159414
35	30.339976309358846	28.85618948350587	21.996222678152407	18.807611528982882
36	21.08033486010208	32.835991737758825	23.774494926283257	22.309178475855838
37	22.396240714950945	33.92955936448034	23.952174734954447	19.722025185614267
38	19.543651653922396	27.709638761080058	23.4952714104603	29.251438174537242
39	24.824618148834805	25.180237912309945	29.437269228701403	20.55787471015385
40	17.337872594298638	24.33883860360493	32.147471639735755	26.17581716236067
41	19.053536339813284	32.315005749229535	24.93578726289848	23.6956706480587
42	19.829378823064207	25.09742472671649	27.995972937864966	27.077223512354344
43	22.298946061300835	27.455996281644605	30.116337350567722	20.12872030648683
44	21.79036037176617	31.88741222235904	22.308224606702403	24.01400279917239
45	24.21917138253795	28.51028184232023	25.126214232074627	22.14433254306719
46	25.964231641053836	27.440127367546594	25.964578502564173	20.631062488835394
47	19.587269488847536	31.533786912568353	27.551036335477512	21.327907263106596
48	21.388347881283178	27.622056229719437	27.066904382421754	23.922691506575628
49	19.128111564536184	27.56170232692044	30.599081857582135	22.711104250961238
50	18.066108335255525	28.062830493982823	30.861742736286395	23.009318434475258
51	18.76832946293698	24.89364358939228	27.96839744779301	28.36962949987773
52	20.040097190595198	24.579647207157834	28.758547968345415	26.621707633901547
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1317.0
1	1374.5
2	1432.0
3	6309.0
4	11186.0
5	6675.5
6	2165.0
7	2725.0
8	3285.0
9	4626.5
10	5968.0
11	7211.5
12	8455.0
13	8741.5
14	8268.0
15	7508.0
16	6568.0
17	5628.0
18	5081.0
19	4534.0
20	4304.0
21	4074.0
22	3892.0
23	3710.0
24	3767.5
25	3825.0
26	4472.0
27	5119.0
28	5906.5
29	6694.0
30	7723.0
31	8752.0
32	10461.0
33	12170.0
34	16230.0
35	20290.0
36	22656.5
37	25023.0
38	27751.5
39	32218.0
40	33956.0
41	45800.0
42	57644.0
43	70165.5
44	82687.0
45	93466.5
46	104246.0
47	114788.0
48	125330.0
49	141684.5
50	158039.0
51	139406.5
52	120774.0
53	97917.5
54	75061.0
55	65821.5
56	56582.0
57	52774.5
58	48967.0
59	44371.0
60	39775.0
61	33318.0
62	26861.0
63	22673.5
64	13923.0
65	9360.0
66	7557.5
67	5755.0
68	4662.0
69	3569.0
70	2890.5
71	2212.0
72	1933.0
73	1654.0
74	1229.5
75	805.0
76	595.5
77	386.0
78	262.0
79	138.0
80	147.5
81	157.0
82	109.5
83	62.0
84	53.0
85	44.0
86	24.0
87	4.0
88	2.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1153198.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.000480255482014
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.62554641274768	17.190475124672613
2	11.826608046346495	5.676885458113276
3	4.616201837270905	3.3237318315222044
4	2.262509535150071	2.1720526170483625
5	1.3541639460373365	1.625029252477735
6	0.9390266710159905	1.3522254646254168
7	0.6770724012948384	1.1375043959165994
8	0.5339515882753556	1.0252075641428748
9	0.452101433234095	0.9765586369629017
>10	4.146611942511753	21.91845451224335
>50	1.0045923114631632	17.257216590172547
>100	0.5467390885665515	18.847877568217125
>500	0.008707191855074296	1.4164272421206021
>1k	0.004716395588165244	2.2677464248963526
>5k	0.001088398981884287	2.5626649144188507
>10k+	3.627996606280957E-4	1.2499424024492067
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	14355	1.2447992452293535	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	9989	0.8661999066942536	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9752	0.8456483622066635	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	9690	0.840272008796408	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4303	0.3731362697472594	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4153	0.36012896310954406	No Hit
CTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCTT	2877	0.24948014131137933	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT	2275	0.1972774840053486	No Hit
GCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGCT	2186	0.1895598154003042	No Hit
GGGTAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1774	0.15383307983537953	No Hit
ACCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCGTCTTCTGC	1370	0.118800067291133	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGGAGCTTCGTATGCCG	1338	0.11602517520842041	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	1289	0.11177612170676675	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	1197	0.10379830696896804	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.25546350236472837	0.0
2	0.0	0.0	0.0	0.7950065816971587	0.0
3	0.0	0.0	0.0	1.4572519203120367	0.0
4	0.0	0.0	0.0	1.9522232955658958	0.0
5	0.0	0.0	0.0	4.453094785110623	0.0
6	0.0	0.0	0.0	4.976855665722625	0.0
7	0.0	0.0	0.0	6.366816453028881	0.0
8	0.0	0.0	0.0	8.214374287849962	0.0
9	0.0	0.0	0.0	9.564532716844809	0.0
10	0.0	0.0	0.0	10.793549763353735	0.0
11	0.0	0.0	0.0	11.925358871590134	0.0
12	0.0	0.0	0.0	12.76667146491756	0.0
13	0.0	0.0	0.0	13.093328292279383	0.0
14	0.0	0.0	0.0	13.297196144981173	0.0
15	0.0	0.0	0.0	13.926836501624178	0.0
16	0.0	0.0	0.0	14.761818872387916	0.0
17	0.0	0.0	0.0	15.774914628710768	0.0
18	0.0	0.0	0.0	16.242917521535762	0.0
19	0.0	0.0	0.0	16.74725415756878	0.0
20	0.0	0.0	0.0	17.367182391922288	0.0
21	0.0	0.0	0.0	17.999771071403178	0.0
22	0.0	0.0	0.0	18.646407642052797	0.0
23	0.0	0.0	0.0	19.08163212215075	0.0
24	0.0	0.0	0.0	19.44843816933432	0.0
25	0.0	0.0	0.0	19.806399248004247	0.0
26	0.0	0.0	0.0	20.253156873320975	0.0
27	0.0	0.0	0.0	20.693150699186088	0.0
28	0.0	0.0	0.0	21.1508344620785	0.0
29	0.0	0.0	0.0	21.57417893544734	0.0
30	0.0	0.0	0.0	22.031082259941485	0.0
31	0.0	0.0	0.0	22.366757486572123	0.0
32	0.0	0.0	0.0	22.78637319870482	0.0
33	0.0	0.0	0.0	23.23096293958193	0.0
34	0.0	0.0	0.0	23.830946637090943	0.0
35	0.0	0.0	0.0	24.210846706289814	0.0
36	0.0	0.0	0.0	24.620923726888183	0.0
37	0.0	0.0	0.0	25.192464780549393	0.0
38	0.0	0.0	0.0	26.39910925964145	0.0
39	0.0	0.0	0.0	26.88922457375056	0.0
40	0.0	0.0	0.0	27.314476785426265	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAC	20	6.3114637E-4	46.0	14
CGTGGAT	20	6.3114637E-4	46.0	40
CGGCCTA	20	6.3114637E-4	46.0	5
CGCATCG	20	6.3114637E-4	46.0	20
GGCCGTA	20	6.3114637E-4	46.0	16
TTCACGT	20	6.3114637E-4	46.0	9
TCGGGTA	45	3.092282E-10	46.0	4
CCGCTTG	20	6.3114637E-4	46.0	38
TGCGGAT	50	1.6370905E-11	46.0	26
ACCTCGC	20	6.3114637E-4	46.0	18
GCCGATT	25	3.4171626E-5	46.0	8
CTAACGG	150	0.0	46.0	1
ACGATAC	20	6.3114637E-4	46.0	46
CTCGGTA	20	6.3114637E-4	46.0	31
AACGCGA	20	6.3114637E-4	46.0	17
AGCGCCG	20	6.3114637E-4	46.0	17
AGCGCAT	20	6.3114637E-4	46.0	32
GATATCG	20	6.3114637E-4	46.0	14
GAACGTT	20	6.3114637E-4	46.0	8
CGAATGC	45	3.092282E-10	46.0	45
>>END_MODULE