##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933078.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1746899
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60947770878568	33.0	31.0	34.0	31.0	34.0
2	32.977852755082004	34.0	31.0	34.0	31.0	34.0
3	33.0616246274112	34.0	33.0	34.0	31.0	34.0
4	36.451158882110526	37.0	37.0	37.0	35.0	37.0
5	36.4324045064998	37.0	37.0	37.0	35.0	37.0
6	36.56524904988783	37.0	37.0	37.0	35.0	37.0
7	36.61091454056588	37.0	37.0	37.0	35.0	37.0
8	36.47220188459665	37.0	37.0	37.0	35.0	37.0
9	38.141458092311005	39.0	39.0	39.0	37.0	39.0
10	37.98003376268462	39.0	38.0	39.0	35.0	39.0
11	37.60096948936373	39.0	37.0	39.0	35.0	39.0
12	37.49390949333648	39.0	37.0	39.0	35.0	39.0
13	37.51200613200878	39.0	37.0	39.0	35.0	39.0
14	38.933626958398854	41.0	38.0	41.0	35.0	41.0
15	38.97048999398363	41.0	38.0	41.0	35.0	41.0
16	38.948426325734914	41.0	38.0	41.0	35.0	41.0
17	38.95204130290303	41.0	38.0	41.0	35.0	41.0
18	38.895584690357026	41.0	38.0	41.0	35.0	41.0
19	38.82301667125575	41.0	37.0	41.0	35.0	41.0
20	38.72568877765686	41.0	37.0	41.0	35.0	41.0
21	38.66835976206982	40.0	37.0	41.0	35.0	41.0
22	38.64168277616508	40.0	37.0	41.0	35.0	41.0
23	38.59953666468411	40.0	36.0	41.0	35.0	41.0
24	38.566447172961915	40.0	36.0	41.0	35.0	41.0
25	38.52757371777075	40.0	36.0	41.0	35.0	41.0
26	38.44016969498523	40.0	36.0	41.0	35.0	41.0
27	38.411041508410044	40.0	36.0	41.0	35.0	41.0
28	38.43813523277534	40.0	36.0	41.0	35.0	41.0
29	38.416790552859666	40.0	36.0	41.0	35.0	41.0
30	38.35802527793536	40.0	36.0	41.0	35.0	41.0
31	38.279715656142685	40.0	36.0	41.0	35.0	41.0
32	38.21058114979744	40.0	36.0	41.0	35.0	41.0
33	38.09214041567372	40.0	35.0	41.0	35.0	41.0
34	37.956265931802584	40.0	35.0	41.0	34.0	41.0
35	37.88271789038748	40.0	35.0	41.0	34.0	41.0
36	37.81741417219885	40.0	35.0	41.0	34.0	41.0
37	37.71262734708761	40.0	35.0	41.0	34.0	41.0
38	37.71706320743214	40.0	35.0	41.0	34.0	41.0
39	37.633023431806876	40.0	35.0	41.0	34.0	41.0
40	37.628678017446916	40.0	35.0	41.0	34.0	41.0
41	37.57977078239784	40.0	35.0	41.0	34.0	41.0
42	37.54758231586371	40.0	35.0	41.0	34.0	41.0
43	37.45908435461924	40.0	35.0	41.0	34.0	41.0
44	37.39118804235391	40.0	35.0	41.0	34.0	41.0
45	37.32163622510517	40.0	35.0	41.0	34.0	41.0
46	37.242561819544235	39.0	35.0	41.0	33.0	41.0
47	37.167492797236704	39.0	35.0	41.0	33.0	41.0
48	37.11702966227584	39.0	35.0	41.0	33.0	41.0
49	37.039024007684475	39.0	35.0	41.0	33.0	41.0
50	36.928281486222154	39.0	35.0	41.0	33.0	41.0
51	36.82746283557321	39.0	35.0	41.0	33.0	41.0
52	36.59297532370217	38.0	35.0	40.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	1.0
13	6.0
14	6.0
15	18.0
16	40.0
17	93.0
18	182.0
19	315.0
20	587.0
21	1013.0
22	1598.0
23	2558.0
24	4054.0
25	6658.0
26	9516.0
27	11274.0
28	12412.0
29	13864.0
30	16112.0
31	20353.0
32	26075.0
33	36079.0
34	86340.0
35	215038.0
36	84491.0
37	120979.0
38	212468.0
39	856616.0
40	8151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.763028085767985	22.263908789231664	25.15245586608041	18.82060725891995
2	26.51114918492712	23.749512707947055	27.855416941677795	21.88392116544803
3	27.310565751082343	25.371930489398643	25.97471290555436	21.342790853964654
4	25.380230912033262	28.814831309652135	23.757068954759262	22.04786882355534
5	22.78477462062775	37.66205144086751	20.975568707750135	18.577605230754614
6	90.14493682805931	2.651784676732885	5.195950080685832	2.0073284145219614
7	92.08282791392061	1.8463574597043102	4.053582948985603	2.017231677389477
8	85.45113369462115	4.469119279363031	6.7674204404490474	3.3123265855667676
9	44.61763387579934	32.07838575670374	11.348395070350376	11.955585297146545
10	31.43656273201828	22.659237883815837	28.214338665257692	17.68986071890819
11	30.197738964874326	22.182793624588484	28.775447235358197	18.84402017517899
12	21.577091749437148	30.06905379189066	29.145646084862374	19.20820837380982
13	17.866344877408483	33.123380344255736	29.92039036028986	19.089884418045923
14	17.24501530998644	27.304955810267224	36.99761692003945	18.452411959706886
15	20.193210941216407	22.962632642184808	36.8942909693119	19.94986544728688
16	19.626435186006745	23.44125218458537	28.213136535083024	28.719176094324855
17	21.60147781869473	25.142266381742733	31.04718704401342	22.20906875554912
18	24.398434025092463	26.830801322801147	27.84402532716545	20.92673932494094
19	25.397461444536862	24.284174414204827	27.706696265782966	22.61166787547534
20	24.3036947184697	26.404159599381533	28.108264988416614	21.183880693732153
21	22.233912779158953	23.695645827263053	27.63256490501168	26.43787648856631
22	19.75483413752026	28.00133264716506	26.7879825908653	25.455850624449383
23	18.56363762301083	26.61510482288902	33.96922203287082	20.852035521229332
24	21.65419981349809	25.685571976399324	31.067050814042485	21.593177396060103
25	19.441994070636024	29.673495720130354	27.04323489795346	23.84127531128016
26	18.153882966330624	29.651342178340016	29.62037301526877	22.574401840060588
27	18.96749611740576	27.37862921668625	33.34674757956814	20.30712708633985
28	18.46162829104602	26.019019989135035	30.813229614305122	24.706122105513828
29	20.160524449324203	28.772699509244664	30.116509311643085	20.95026672978804
30	26.229564502584296	27.671891735011585	24.921704116837894	21.17683964556623
31	24.20912714472903	28.39494441292828	26.018905500546968	21.37702294179572
32	24.665020702398937	28.329743162025967	24.9870771006223	22.018159034952795
33	20.66788062732877	27.71505393271162	26.860167645639503	24.756897794320107
34	20.588997990152833	26.454420089541525	29.020510058108684	23.936071862196957
35	26.816490249293174	26.489110131724846	25.770236287272475	20.924163331709504
36	22.03996911097894	28.323503533976492	27.912947457179836	21.72357989786473
37	21.439361977996438	29.676529667714046	27.281256672538024	21.602851681751492
38	21.22744360149041	27.14369863397941	26.748369539395238	24.88048822513494
39	22.810820774412257	25.083877201830212	28.322301403801823	23.783000619955704
40	18.695986430812543	25.15131098019977	30.493405743548998	25.65929684543869
41	20.911684075610555	27.485618802231844	25.63124714136307	25.971449980794542
42	21.817746761547177	26.01661572878569	26.852439665945198	25.313197843721934
43	21.64813191833071	27.440395809946654	28.044895554923322	22.86657671679931
44	21.375706323032986	30.47623245533943	25.944659651187617	22.20340157043996
45	22.977802380103256	27.876769063351688	26.537309827299687	22.608118729245366
46	22.86840853420833	26.452760005014603	27.30003280098048	23.378798659796587
47	21.057141826745564	26.531356420720375	29.324477259417975	23.087024493116086
48	21.312737599597916	25.640406228408168	28.55820513950721	24.488651032486707
49	21.354525934241188	26.76920646242284	29.70973135825254	22.16653624508343
50	20.597470145669554	26.48321396943956	28.6806506844414	24.238665200449482
51	21.40501540157731	23.95833989257536	28.918214504673713	25.71843020117362
52	21.88569573856302	23.873961803172364	29.06767935639095	25.172663101873667
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1691.0
1	1903.5
2	2116.0
3	5805.5
4	9495.0
5	6215.5
6	2936.0
7	3168.0
8	3400.0
9	3558.0
10	3716.0
11	3849.0
12	3982.0
13	3955.0
14	4097.0
15	4266.0
16	3915.0
17	3564.0
18	3508.5
19	3453.0
20	4078.5
21	4704.0
22	5491.0
23	6278.0
24	6880.0
25	7482.0
26	10110.0
27	12738.0
28	14575.0
29	16412.0
30	18013.5
31	19615.0
32	24967.0
33	30319.0
34	35838.5
35	41358.0
36	45347.0
37	49336.0
38	53333.5
39	61450.0
40	65569.0
41	81843.5
42	98118.0
43	117626.5
44	137135.0
45	148171.5
46	159208.0
47	160438.5
48	161669.0
49	167159.5
50	172650.0
51	160787.5
52	148925.0
53	131636.0
54	114347.0
55	103376.5
56	92406.0
57	84461.0
58	76516.0
59	73518.5
60	70521.0
61	63326.5
62	56132.0
63	46352.5
64	30913.5
65	25254.0
66	20755.0
67	16256.0
68	13577.5
69	10899.0
70	9022.5
71	7146.0
72	5886.5
73	4627.0
74	3640.0
75	2653.0
76	1859.5
77	1066.0
78	852.5
79	639.0
80	493.5
81	348.0
82	195.5
83	43.0
84	45.0
85	47.0
86	30.0
87	13.0
88	10.5
89	7.0
90	6.0
91	4.5
92	3.0
93	1.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1746899.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.49948749335017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.22652408982712	13.176541915945613
2	12.030928118628562	4.451320085279279
3	4.560955228667019	2.5312600263136678
4	2.272917094256529	1.681912054344819
5	1.3000111612757426	1.2024770109618115
6	0.8358579263424565	0.9277765952753928
7	0.5731751808761903	0.7422412963082462
8	0.40760337442699013	0.6032362821967545
9	0.32970700814104914	0.5489469606217717
>10	3.6195108398062947	16.12253163039688
>50	1.5587705860175751	21.057801082121173
>100	1.274394317553907	34.17558640208498
>500	0.006222628503604124	0.7909581757223351
>1k	0.0028001828266218556	1.258729758759405
>5k	6.222628503604123E-4	0.7286807236679349
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	6634	0.3797586466075028	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6026	0.34495411583611874	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4817	0.27574576435157383	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3873	0.22170715078547756	No Hit
CTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCTT	2736	0.15662038847122817	TruSeq Adapter, Index 22 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	2269	0.12988730315833943	TruSeq Adapter, Index 20 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAAAGGTCCTCGTATGCCGTCTTCTGCT	2148	0.12296074358048176	TruSeq Adapter, Index 20 (95% over 22bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2124	0.12158688052371659	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1627455279326395	0.0
2	0.0	0.0	0.0	0.5086727967673003	0.0
3	0.0	0.0	0.0	0.8823063039133917	0.0
4	0.0	0.0	0.0	1.1357840378865636	0.0
5	0.0	0.0	0.0	2.378614905612746	0.0
6	0.0	0.0	0.0	2.6106260293239623	0.0
7	0.0	0.0	0.0	3.3105520124517787	0.0
8	0.0	0.0	0.0	4.246496219873044	0.0
9	0.0	0.0	0.0	4.9803680693617665	0.0
10	0.0	0.0	0.0	5.590191533683401	0.0
11	0.0	0.0	0.0	6.240028759533321	0.0
12	0.0	0.0	0.0	6.734733948556843	0.0
13	0.0	0.0	0.0	6.937149772253576	0.0
14	0.0	0.0	0.0	7.068296449880617	0.0
15	0.0	0.0	0.0	7.5585938282636835	0.0
16	0.0	0.0	0.0	8.205969549470232	0.0
17	0.0	0.0	0.0	8.978424053136443	0.0
18	0.0	0.0	0.0	9.376042919481893	0.0
19	0.0	0.0	0.0	9.786885217748708	0.0
20	0.0	0.0	0.0	10.258635444865444	0.0
21	5.7244294031881636E-5	0.0	0.0	10.77142983080304	0.0
22	5.7244294031881636E-5	0.0	0.0	11.294928899724598	0.0
23	5.7244294031881636E-5	0.0	0.0	11.7114956273946	0.0
24	5.7244294031881636E-5	0.0	0.0	12.053644772823157	0.0
25	5.7244294031881636E-5	0.0	0.0	12.38657758691258	0.0
26	5.7244294031881636E-5	0.0	0.0	12.701707425558089	0.0
27	5.7244294031881636E-5	0.0	0.0	13.04402830386874	0.0
28	5.7244294031881636E-5	0.0	0.0	13.405640509268137	0.0
29	5.7244294031881636E-5	0.0	0.0	13.828046154929392	0.0
30	5.7244294031881636E-5	0.0	0.0	14.185365038276398	0.0
31	5.7244294031881636E-5	0.0	0.0	14.523278105946595	0.0
32	5.7244294031881636E-5	0.0	0.0	14.878936904766675	0.0
33	5.7244294031881636E-5	0.0	0.0	15.234023260646437	0.0
34	1.1448858806376327E-4	0.0	0.0	15.685852473440079	0.0
35	1.1448858806376327E-4	0.0	0.0	16.031665253686675	0.0
36	1.1448858806376327E-4	0.0	0.0	16.401864103190853	0.0
37	1.1448858806376327E-4	0.0	0.0	16.7484783035539	0.0
38	1.1448858806376327E-4	0.0	0.0	17.108831134484593	0.0
39	1.1448858806376327E-4	0.0	0.0	17.517841615342387	0.0
40	1.1448858806376327E-4	0.0	0.0	17.898115460596177	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	20	6.312359E-4	46.000004	27
AATGCGT	20	6.312359E-4	46.000004	9
ATATCCG	20	6.312359E-4	46.000004	35
ACTCGTA	20	6.312359E-4	46.000004	41
TCGTATA	35	1.0199801E-7	46.000004	40
ATCGGCA	20	6.312359E-4	46.000004	9
TCGAATC	20	6.312359E-4	46.000004	46
ATTACGT	25	3.4178905E-5	46.0	24
TAAGCGC	25	3.4178905E-5	46.0	18
GTACGTC	25	3.4178905E-5	46.0	40
CGGTCTA	410	0.0	45.439026	30
CACGACG	420	0.0	44.904762	25
TACGGGA	855	0.0	44.11696	3
CTTACGG	225	0.0	43.955555	1
CCGACGG	105	0.0	43.809525	1
CTAACGG	190	0.0	43.57895	1
ACTACGG	90	0.0	43.444447	1
CTAGCGG	505	0.0	42.811886	1
CTTGCGG	490	0.0	42.714287	1
ACGGATA	65	0.0	42.46154	9
>>END_MODULE