##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933076.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1048525
Sequences flagged as poor quality	0
Sequence length	52
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54131661143034	33.0	31.0	34.0	31.0	34.0
2	32.89494480341432	34.0	31.0	34.0	31.0	34.0
3	32.931547650270616	34.0	31.0	34.0	31.0	34.0
4	36.37439069168594	37.0	37.0	37.0	35.0	37.0
5	36.35968622588875	37.0	37.0	37.0	35.0	37.0
6	36.50424930259174	37.0	37.0	37.0	35.0	37.0
7	36.56749719844544	37.0	37.0	37.0	35.0	37.0
8	36.41593858038674	37.0	37.0	37.0	35.0	37.0
9	38.044223075272406	39.0	39.0	39.0	35.0	39.0
10	37.966453827996475	39.0	38.0	39.0	35.0	39.0
11	37.68231658758732	39.0	37.0	39.0	35.0	39.0
12	37.596924250733174	39.0	37.0	39.0	35.0	39.0
13	37.52975274790778	39.0	37.0	39.0	35.0	39.0
14	39.003984645096686	41.0	38.0	41.0	35.0	41.0
15	39.062246489115665	41.0	38.0	41.0	35.0	41.0
16	39.05003218807372	41.0	38.0	41.0	35.0	41.0
17	39.02874418826447	41.0	38.0	41.0	35.0	41.0
18	38.9132734078825	41.0	38.0	41.0	35.0	41.0
19	38.75644643666102	40.0	37.0	41.0	35.0	41.0
20	38.6052101761999	40.0	36.0	41.0	35.0	41.0
21	38.56785484370902	40.0	36.0	41.0	35.0	41.0
22	38.52486111442264	40.0	36.0	41.0	35.0	41.0
23	38.48082973701152	40.0	36.0	41.0	35.0	41.0
24	38.40517488853389	40.0	36.0	41.0	35.0	41.0
25	38.34349204835364	40.0	35.0	41.0	35.0	41.0
26	38.25119572733125	40.0	35.0	41.0	35.0	41.0
27	38.17855082139196	40.0	35.0	41.0	35.0	41.0
28	38.14071481366682	40.0	35.0	41.0	35.0	41.0
29	38.087695572351635	40.0	35.0	41.0	34.0	41.0
30	38.002343291767005	40.0	35.0	41.0	34.0	41.0
31	37.904960778236095	40.0	35.0	41.0	34.0	41.0
32	37.75624520159271	40.0	35.0	41.0	34.0	41.0
33	37.51694427886793	40.0	35.0	41.0	33.0	41.0
34	37.35444266946425	40.0	35.0	41.0	33.0	41.0
35	37.2006265945018	40.0	35.0	41.0	33.0	41.0
36	37.12284208769462	40.0	35.0	41.0	33.0	41.0
37	36.99418993347798	40.0	35.0	41.0	33.0	41.0
38	36.94289597291433	40.0	35.0	41.0	33.0	41.0
39	36.85581841157817	40.0	35.0	41.0	32.0	41.0
40	36.78823299396772	40.0	35.0	41.0	32.0	41.0
41	36.74108867218235	40.0	35.0	41.0	32.0	41.0
42	36.684528265897335	39.0	35.0	41.0	32.0	41.0
43	36.60303092439379	39.0	35.0	41.0	32.0	41.0
44	36.504196847953075	39.0	35.0	41.0	31.0	41.0
45	36.429227724660834	39.0	35.0	41.0	31.0	41.0
46	36.343328008392746	39.0	35.0	41.0	31.0	41.0
47	36.236394935743064	39.0	35.0	41.0	31.0	41.0
48	36.16698123554517	39.0	35.0	41.0	31.0	41.0
49	36.08768603514461	38.0	35.0	41.0	31.0	41.0
50	35.97301542643237	38.0	35.0	41.0	31.0	41.0
51	35.852832312057416	38.0	35.0	41.0	30.0	41.0
52	35.61674256693927	37.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	4.0
14	4.0
15	22.0
16	33.0
17	82.0
18	170.0
19	327.0
20	570.0
21	935.0
22	1474.0
23	2466.0
24	4321.0
25	7325.0
26	10438.0
27	12429.0
28	12391.0
29	12513.0
30	12961.0
31	15171.0
32	18412.0
33	24132.0
34	52096.0
35	98731.0
36	73179.0
37	82927.0
38	131928.0
39	468291.0
40	5189.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.679144512529504	20.716434992012587	24.108438043918838	22.495982451539067
2	30.74394983429103	22.740993300112063	24.863975584750005	21.651081280846903
3	27.172265801959895	23.145943110560072	28.65253570491881	21.029255382561217
4	23.317231348799506	26.61319472592451	26.716387305977445	23.353186619298537
5	21.475596671514747	36.52912424596457	24.411721227438544	17.583557855082137
6	85.00236045873966	2.721442025702773	10.350540044348012	1.9256574712095562
7	86.5513936243771	2.191745547316468	8.726544431463246	2.5303163968431845
8	79.25161536444053	4.3358050594883295	12.200853580029088	4.211725996042059
9	37.07722753391669	28.89420853103169	18.037624281729094	15.990939653322526
10	29.66004625545409	23.811926277389666	28.647862473474646	17.8801649936816
11	27.230156648625446	21.075892324932642	32.109963997043465	19.583987029398443
12	22.45659378650962	23.63014711141842	34.26480055315801	19.64845854891395
13	18.073293436017263	28.516153644405236	34.91151856178918	18.49903435778832
14	17.39138313344937	27.44703273646313	36.194749767530574	18.966834362556924
15	20.695834624830116	22.640757254238096	36.854819866002245	19.808588254929543
16	20.059512171860472	24.62506854867552	31.48193891418898	23.83348036527503
17	20.86006532986815	26.389737965236883	31.54383538780668	21.20636131708829
18	23.204120073436492	29.443265539686703	29.18747764717103	18.16513673970578
19	22.9201974201855	26.537183185904006	28.861114422641332	21.681504971269163
20	22.123077656708233	27.38351493765051	31.29443742400038	19.198969981640875
21	19.3800815431201	24.370329748933024	32.06075200877423	24.188836699172647
22	18.393838964259317	28.389022674709715	29.832002098185544	23.38513626284543
23	18.063183996566607	29.107365108128086	32.489640208864834	20.339810686440476
24	22.834648673136073	25.483989413700197	30.744140578431605	20.937221334732122
25	18.064614577620944	28.492310626832936	29.380510717436398	24.062564078109723
26	17.24899263250757	26.836937602823014	32.23823943158246	23.675830333086957
27	20.129324527312175	26.142628931117525	33.22410052216209	20.503946019408218
28	19.53334446007487	27.68880093464629	30.151689277794997	22.626165327483847
29	20.38930879092058	25.615602870699316	31.281752938651913	22.71333539972819
30	25.11203833957226	26.140912233852315	28.321785365155815	20.42526406141961
31	22.701890751293483	28.02794401659474	26.834267184854916	22.43589804725686
32	22.705324145823898	26.584296988626882	27.232445578312394	23.477933287236834
33	19.362246965976013	28.329129014568082	28.634415011563863	23.67420900789204
34	19.162633222860688	28.160129706015592	28.18111156147922	24.4961255096445
35	23.118857442597935	28.959252282968933	26.962351875253333	20.9595383991798
36	19.617653370210533	30.028563935051622	28.412674948141436	21.94110774659641
37	20.594263369972104	32.150973987267825	27.286712286306958	19.968050356453112
38	18.740421067690328	28.78920388164326	27.644452921961804	24.825922128704608
39	21.114518013399774	27.333826089029827	30.898357216089266	20.65329868148113
40	18.154455067833386	26.959109224863496	31.883932190458026	23.002503516845092
41	19.333253856608092	30.471281085334162	27.36300994253833	22.83245511551942
42	19.773300588922535	25.766099997615697	30.163706158651436	24.29689325481033
43	21.360005722324217	26.35588088028421	30.662120597982877	21.621992799408694
44	20.657113564292697	28.356882287022245	27.96261414844663	23.02339000023843
45	21.566200138289503	27.72151355475549	28.314250971602966	22.39803533535204
46	21.865096206575902	27.953267685558288	28.554970077012946	21.626666030852864
47	19.05476741136358	29.453947211559097	30.570086550153786	20.921198826923533
48	20.214014925729	27.2058367707017	29.152380725304596	23.427767578264707
49	19.368446150544813	26.34605755704442	31.580935123149185	22.70456116926158
50	18.890059845974104	27.056197992417918	31.45151522376672	22.60222693784125
51	19.263822989437543	25.407405641257956	30.04039007176748	25.288381297537015
52	19.140506902553586	24.996638134522307	31.409074652488016	24.453780310436088
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1767.0
1	1913.0
2	2059.0
3	13777.0
4	25495.0
5	15358.0
6	5221.0
7	5640.5
8	6060.0
9	6834.0
10	7608.0
11	8635.5
12	9663.0
13	9806.5
14	9180.0
15	8410.0
16	7717.0
17	7024.0
18	6501.0
19	5978.0
20	5595.0
21	5212.0
22	5330.0
23	5448.0
24	5804.5
25	6161.0
26	6709.5
27	7258.0
28	8512.5
29	9767.0
30	10830.5
31	11894.0
32	13830.5
33	15767.0
34	17945.0
35	20123.0
36	22431.5
37	24740.0
38	27111.5
39	31707.5
40	33932.0
41	41093.5
42	48255.0
43	57392.0
44	66529.0
45	76990.5
46	87452.0
47	91069.5
48	94687.0
49	95978.5
50	97270.0
51	87910.5
52	78551.0
53	69249.0
54	59947.0
55	56854.5
56	53762.0
57	51714.0
58	49666.0
59	46324.0
60	42982.0
61	38154.5
62	33327.0
63	29508.5
64	21637.0
65	17584.0
66	14288.0
67	10992.0
68	9251.0
69	7510.0
70	6435.5
71	5361.0
72	5189.5
73	5018.0
74	3758.5
75	2499.0
76	1794.0
77	1089.0
78	854.5
79	620.0
80	540.0
81	460.0
82	308.0
83	156.0
84	104.5
85	53.0
86	34.0
87	15.0
88	13.5
89	14.5
90	17.0
91	9.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1048525.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.99110638176742
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.42423352721298	22.020739987143788
2	9.492029328687462	5.6935292275104405
3	3.535502443751427	3.181008897106431
4	1.8943937099154988	2.2725985313210706
5	1.1921760208328562	1.787733893329518
6	0.9081316573247107	1.6341523886085692
7	0.7172418134707583	1.5057612870477308
8	0.6363985355965307	1.5269036945821242
9	0.561248384019325	1.5149213992546766
>10	7.3915981801943325	47.55751505038922
>50	0.18704040290132676	3.6249539807513087
>100	0.052664836996470905	2.7933763895173604
>500	0.0038301699633492	0.7534582626293687
>1k	0.0028726274725119003	1.6807546913030689
>5k	3.191808302791E-4	0.5862251810878228
>10k+	3.191808302791E-4	1.8663671384175837
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19497	1.8594692544288405	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	6124	0.5840585584511576	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3456	0.3296058749195298	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3326	0.3172075057819318	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1880	0.1792994921437257	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCG	1740	0.16594740230323551	No Hit
CTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCTT	1609	0.15345366109534822	Illumina Single End Adapter 1 (95% over 21bp)
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1527	0.14563315133163254	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1493	0.1423905009417992	No Hit
CCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	1465	0.13972008297370114	No Hit
GCTGTCTCTTATACACATCTGACGCCGCTAAATTCGTATGCCGTCTTCTGCT	1062	0.10128513864714718	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15898524117212273	0.0
2	0.0	0.0	0.0	0.4833456522257457	0.0
3	0.0	0.0	0.0	0.9424667985980306	0.0
4	0.0	0.0	0.0	1.2879044371855701	0.0
5	0.0	0.0	0.0	3.0610619680026705	0.0
6	0.0	0.0	0.0	3.5075940010967788	0.0
7	0.0	0.0	0.0	4.502515438353878	0.0
8	0.0	0.0	0.0	5.820938938031997	0.0
9	0.0	0.0	0.0	6.947855320569372	0.0
10	0.0	0.0	0.0	7.836818387735152	0.0
11	0.0	0.0	0.0	8.77823609356	0.0
12	0.0	0.0	0.0	9.441071982070051	0.0
13	0.0	0.0	0.0	9.734054981998522	0.0
14	0.0	0.0	0.0	9.933001120621826	0.0
15	0.0	0.0	0.0	10.465368016976228	0.0
16	9.537207028921581E-5	0.0	0.0	11.161011897665768	0.0
17	9.537207028921581E-5	0.0	0.0	11.940583200209819	0.0
18	9.537207028921581E-5	0.0	0.0	12.370711237214183	0.0
19	9.537207028921581E-5	0.0	0.0	12.812474666793829	0.0
20	9.537207028921581E-5	0.0	0.0	13.338451634438854	0.0
21	9.537207028921581E-5	0.0	0.0	13.88626880618011	0.0
22	9.537207028921581E-5	0.0	0.0	14.431606304093846	0.0
23	9.537207028921581E-5	0.0	0.0	14.844948856727308	0.0
24	9.537207028921581E-5	0.0	0.0	15.191721704298896	0.0
25	9.537207028921581E-5	0.0	0.0	15.536014878042964	0.0
26	9.537207028921581E-5	0.0	0.0	15.903674209007892	0.0
27	1.9074414057843162E-4	0.0	0.0	16.28964497746835	0.0
28	2.861162108676474E-4	0.0	0.0	16.70289215803152	0.0
29	2.861162108676474E-4	0.0	0.0	17.096778808325983	0.0
30	2.861162108676474E-4	0.0	0.0	17.468348394172768	0.0
31	2.861162108676474E-4	0.0	0.0	17.809112801316136	0.0
32	2.861162108676474E-4	0.0	0.0	18.183829665482463	0.0
33	3.8148828115686323E-4	0.0	0.0	18.56388736558499	0.0
34	3.8148828115686323E-4	0.0	0.0	19.01022865453852	0.0
35	3.8148828115686323E-4	0.0	0.0	19.37598054409766	0.0
36	3.8148828115686323E-4	0.0	0.0	19.763286521542167	0.0
37	3.8148828115686323E-4	0.0	0.0	20.187596862258886	0.0
38	3.8148828115686323E-4	0.0	0.0	20.838415869912495	0.0
39	3.8148828115686323E-4	0.0	0.0	21.271977301447272	0.0
40	3.8148828115686323E-4	0.0	0.0	21.670823299396773	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGG	35	1.0194526E-7	46.000004	1
ATGACGG	80	0.0	46.0	1
TACTCGC	25	3.4169487E-5	46.0	44
AATCGGC	20	6.3111994E-4	46.0	46
ACGTAGG	130	0.0	44.23077	1
CGTTTTT	6150	0.0	44.09268	1
CCAATCG	95	0.0	43.578945	23
TAACCGG	95	0.0	43.578945	1
CTTGCGG	290	0.0	42.827587	1
ATTGCGG	275	0.0	42.65455	1
TCACGAC	270	0.0	42.592594	24
CACGACG	260	0.0	42.46154	25
CGCACTT	2295	0.0	42.29194	34
ATAGCGG	120	0.0	42.166668	1
GCACTTA	2310	0.0	42.017315	35
TCGACGG	115	0.0	42.0	1
ACCCGCA	2370	0.0	41.92405	31
TCAACGC	2530	0.0	41.909092	15
CTCGCGG	110	0.0	41.81818	1
CCCGCAC	2380	0.0	41.747894	32
>>END_MODULE