FastQCFastQC Report
Sat 14 Jan 2017
SRR2933074.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933074.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724642
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199962.7594315537879397No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC196352.7096138507014498No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG79201.0929534859972234No Hit
GGGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT35420.4887930867932027RNA PCR Primer, Index 38 (95% over 21bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCCGACTATGTCG21360.29476624319319056No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA20580.2840023073462482No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG20200.2787583386003019No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCTT17560.24232655573372783RNA PCR Primer, Index 38 (96% over 25bp)
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG15440.2130707300984486No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCCGACTATGT15120.20865475641765174No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT14180.19568283373031098RNA PCR Primer, Index 27 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT12800.1766389472318745RNA PCR Primer, Index 38 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT10370.1431051470933233RNA PCR Primer, Index 27 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC9750.1345491980867794RNA PCR Primer, Index 38 (95% over 23bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCC9390.12958122769588293No Hit
GGGGTTGGGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG9130.1259932490802355No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT9000.12419925977241175No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC8800.12143927622191371No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG8630.1190932902039904No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT8520.11757529925121646No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGTCTCTTATACACATCTG8330.11495331487824333No Hit
GGGGTTGGGGATTTAGCTCAGTGGCTGTCTCTTATACACATCTGACGCCGAC7990.11026134284239665No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGCTGTCTCTTATACACATCTGACGCC7600.10487937491892547No Hit
GGGGTTGGGGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCC7530.10391338067625117No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTGTCTCTTATACACATCTGA7340.10129139630327803No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG301.8604442E-646.01
CGGGTAG206.3099037E-446.06
CGTATGG405.6061253E-946.02
CATGCGA301.8604442E-646.011
ACTTGCG301.8604442E-646.01
ACCGCGG253.415897E-546.02
GACGTAG301.8604442E-646.01
CGATTGA206.3099037E-446.010
CGCTCGA206.3099037E-446.044
TACGAAT453.092282E-1046.012
GTCCGGA253.415897E-546.012
CGTAAGG1150.046.02
TATAGCG405.6061253E-946.01
ATGCGAA301.8604442E-646.012
TAATGCG253.415897E-546.01
TACGTAT206.3099037E-446.015
CTTACGG206.3099037E-446.02
CGTTTTT47150.045.268291
TACGGGA1250.044.1599964
AATGCGG850.043.2941172
Produced by FastQC (version 0.11.5)