##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933074.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	724642
Sequences flagged as poor quality	0
Sequence length	52
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38561248174961	33.0	31.0	34.0	31.0	34.0
2	32.74268121361997	34.0	31.0	34.0	31.0	34.0
3	32.7304186067051	34.0	31.0	34.0	31.0	34.0
4	36.23027646755225	37.0	37.0	37.0	35.0	37.0
5	36.03261886559156	37.0	35.0	37.0	35.0	37.0
6	36.14424502029968	37.0	35.0	37.0	35.0	37.0
7	36.475629345249104	37.0	37.0	37.0	35.0	37.0
8	36.44225700414825	37.0	37.0	37.0	35.0	37.0
9	38.251607000422275	39.0	39.0	39.0	37.0	39.0
10	37.929676999125086	39.0	38.0	39.0	35.0	39.0
11	37.56591806712832	39.0	37.0	39.0	35.0	39.0
12	37.140278923937615	39.0	35.0	39.0	35.0	39.0
13	37.0483673317307	39.0	35.0	39.0	34.0	39.0
14	37.34510558317072	40.0	36.0	41.0	27.0	41.0
15	37.821848581782454	40.0	36.0	41.0	32.0	41.0
16	38.19649840886948	40.0	36.0	41.0	33.0	41.0
17	38.310764487843656	40.0	36.0	41.0	34.0	41.0
18	38.247170326864854	40.0	36.0	41.0	34.0	41.0
19	38.15446247940362	40.0	36.0	41.0	34.0	41.0
20	37.860700870222814	40.0	35.0	41.0	33.0	41.0
21	37.84791248644158	40.0	35.0	41.0	33.0	41.0
22	37.84521736250452	40.0	35.0	41.0	34.0	41.0
23	37.875452982300224	40.0	35.0	41.0	34.0	41.0
24	37.803220072808365	40.0	35.0	41.0	34.0	41.0
25	37.60202693191948	39.0	35.0	41.0	34.0	41.0
26	37.53166253129131	39.0	35.0	41.0	34.0	41.0
27	37.42603934080553	39.0	35.0	41.0	34.0	41.0
28	37.382586159786484	39.0	35.0	41.0	34.0	41.0
29	37.29467930371135	39.0	35.0	41.0	33.0	41.0
30	37.23868475743885	39.0	35.0	41.0	33.0	41.0
31	37.04512849103419	39.0	35.0	41.0	33.0	41.0
32	36.90771719000555	39.0	35.0	41.0	33.0	41.0
33	36.77360820929507	39.0	35.0	41.0	32.0	41.0
34	36.51469415242285	39.0	35.0	41.0	31.0	41.0
35	36.44710215527115	39.0	35.0	41.0	31.0	41.0
36	36.3744787081069	39.0	35.0	41.0	31.0	41.0
37	36.222353934770545	39.0	35.0	41.0	31.0	41.0
38	36.22046472603023	39.0	35.0	41.0	31.0	41.0
39	36.170324104868335	39.0	35.0	41.0	31.0	41.0
40	36.069155527833054	38.0	35.0	41.0	31.0	41.0
41	35.86803000654116	38.0	35.0	41.0	30.0	41.0
42	35.93777341087047	38.0	35.0	41.0	30.0	41.0
43	35.918877183491986	38.0	35.0	41.0	30.0	41.0
44	35.91179782568496	38.0	35.0	41.0	30.0	41.0
45	35.879287979443646	38.0	35.0	41.0	30.0	41.0
46	35.848612142271634	38.0	35.0	41.0	30.0	41.0
47	35.749358993820394	38.0	35.0	41.0	30.0	41.0
48	35.60277212747812	37.0	35.0	41.0	29.0	41.0
49	35.58688980213678	37.0	35.0	40.0	29.0	41.0
50	35.4746592110311	37.0	35.0	40.0	29.0	41.0
51	35.40544020357639	37.0	35.0	40.0	29.0	41.0
52	35.14807725745955	36.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	3.0
14	5.0
15	11.0
16	36.0
17	98.0
18	176.0
19	345.0
20	645.0
21	1022.0
22	1569.0
23	2447.0
24	4115.0
25	6901.0
26	8480.0
27	9039.0
28	9240.0
29	10047.0
30	11401.0
31	13736.0
32	17420.0
33	24825.0
34	64869.0
35	72674.0
36	54124.0
37	56060.0
38	79839.0
39	273761.0
40	1752.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.44243088311194	21.650276964349295	25.195475834964025	14.711816317574748
2	41.724327323009156	21.54939956557859	24.246317491947746	12.479955619464508
3	33.52069021668631	21.84306181535158	31.738568838129726	12.897679129832387
4	30.18331810742408	24.77678633035347	31.425310705148195	13.614584857074252
5	23.414596449005167	29.024953011280054	33.40187292483737	14.158577614877416
6	24.856273856607814	35.23257001388272	31.001929228501794	8.90922690100767
7	84.23690594804056	1.424427510412038	11.993508518689229	2.345158022858184
8	82.18265019140486	1.5490407677170244	12.46449971158172	3.8038093292963975
9	77.22889371579346	3.4588113854841422	14.411392108103035	4.900902790619368
10	48.49180698883035	23.256035394029052	18.19146557886515	10.060692038275452
11	30.69460506015384	23.69514877691329	30.481258331700346	15.128987831232527
12	30.448690525804466	16.8894433389177	35.779184756058854	16.88268137921898
13	31.038637009723423	16.85259755851855	38.65412162143513	13.454643810322892
14	16.229393272816097	20.21784550164081	45.82110890619092	17.731652319352175
15	12.656042569986283	28.567209739429956	43.190568584211235	15.586179106372525
16	30.9675674332981	24.86496780479188	30.193254048205876	13.974210713704146
17	26.485492146466804	22.37256465951463	28.34779104716536	22.794152146853204
18	22.986385001145393	23.104650296284234	36.85199036213744	17.05697434043293
19	22.593225344376947	25.536609801805582	29.633253385809823	22.23691146800765
20	14.714576301125245	41.36152748529619	27.673113068246113	16.250783145332452
21	21.004578812710275	26.486734139064534	38.7431310909387	13.765555957286493
22	15.379180340085172	22.97562106529845	42.57095227712443	19.074246317491948
23	18.43048015433828	24.619881265507658	38.808956698618076	18.140681881535986
24	20.738102400909693	31.68571515313769	29.766008594588776	17.810173851363846
25	23.873443714275464	26.108754391823823	31.658391315987757	18.359410577912953
26	15.268229001355152	30.558813869469336	27.190391945264007	26.982565183911504
27	21.368758642198493	25.153938082529027	34.902889978775725	18.574413296496754
28	14.96918478365869	29.837216170191628	27.629919325680817	27.563679720468865
29	20.672966789117936	31.68971712928591	26.047620756180294	21.58969532541586
30	26.57850359211859	24.015168869593538	27.033900877950767	22.372426660337105
31	21.191705697434042	26.92143154826797	32.707875061064634	19.17898769323335
32	26.2526875339823	24.533217782022017	24.986821078546374	24.227273605449312
33	25.942603382083846	24.244247504284875	30.189390071235177	19.62375904239611
34	16.111817973564875	26.61424537909754	36.491122512909826	20.78281413442776
35	19.50894372669539	31.812950394815648	27.228065720728306	21.45004015776066
36	25.966615238973173	26.575743608568093	25.693376867473873	21.76426428498486
37	18.064782333897288	33.80317453307978	26.883757772803673	21.248285360219253
38	22.187783760808784	26.08391453986934	31.552407947648632	20.17589375167324
39	19.035606547784976	28.930147576320447	27.255941554588336	24.778304321306244
40	26.11179037372937	26.14201219360733	30.49050427659451	17.25569315606879
41	18.65900679231952	26.110962378664222	26.64943516936639	28.58059565964987
42	18.261983158580374	28.03135893310076	33.527176178029976	20.179481730288888
43	18.531495552286508	28.645593272264097	32.3740550506319	20.448856124817496
44	24.522315846997554	25.690340885568325	27.356818953359035	22.430524314075086
45	18.71061848471383	24.644307119929564	25.812608156855386	30.832466238501215
46	21.681326779292394	24.489610041924152	29.223671826915908	24.605391351867542
47	16.32502670284085	26.090538500390537	33.464662550611195	24.11977224615741
48	16.858393523974595	33.16962030906296	28.860043994137797	21.111942172824648
49	24.05022066068486	26.729612691508358	29.273627529179926	19.946539118626852
50	19.250057269658676	26.679518990066818	28.610127483640195	25.46029625663431
51	19.33271877699609	27.672423072358487	26.515575967167237	26.479282183478187
52	18.88684343441313	28.84693407227293	28.606539505024546	23.65968298828939
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	577.0
1	1177.0
2	1777.0
3	12147.5
4	22518.0
5	12586.5
6	2655.0
7	3068.5
8	3482.0
9	4676.5
10	5871.0
11	7110.5
12	8350.0
13	9029.0
14	9218.5
15	8729.0
16	8064.0
17	7399.0
18	6685.0
19	5971.0
20	5599.5
21	5228.0
22	4980.5
23	4733.0
24	4970.0
25	5207.0
26	5463.0
27	5719.0
28	6434.5
29	7150.0
30	7889.5
31	8629.0
32	9264.0
33	9899.0
34	10982.5
35	12066.0
36	13048.5
37	14031.0
38	15024.5
39	17267.5
40	18517.0
41	20971.5
42	23426.0
43	27741.5
44	32057.0
45	38511.5
46	44966.0
47	53750.5
48	62535.0
49	72117.5
50	81700.0
51	76569.5
52	71439.0
53	63158.0
54	54877.0
55	49750.5
56	44624.0
57	40465.0
58	36306.0
59	40618.5
60	44931.0
61	29787.0
62	14643.0
63	12342.0
64	8114.5
65	6188.0
66	5266.0
67	4344.0
68	3726.0
69	3108.0
70	2491.0
71	1874.0
72	1577.5
73	1281.0
74	1001.0
75	721.0
76	713.5
77	706.0
78	529.0
79	352.0
80	254.0
81	156.0
82	114.5
83	73.0
84	54.0
85	35.0
86	23.0
87	11.0
88	10.0
89	6.0
90	3.0
91	1.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	724642.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.980467799854395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.50048598139014	23.95639493764714
2	12.073372229018288	8.205176725276244
3	4.8964540055314085	4.991513930052843
4	2.6193734779869966	3.5603014449811905
5	1.5812721594620838	2.6866183848703784
6	1.0944998037423272	2.2314969202807866
7	0.8181081484996992	1.9459788317824773
8	0.6981528499991473	1.8978848351018083
9	0.5856730169206176	1.7911298783443164
>10	4.976984749993651	33.019772437268394
>50	0.10714398159047331	2.2272575374042987
>100	0.03503567432276476	2.2841537153860165
>500	0.00814783123785227	2.085916859602212
>1k	0.004073915618926135	2.53374942137638
>5k	4.0739156189261344E-4	1.0963937833852881
>10k+	8.147831237852269E-4	5.486260357240195
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19996	2.7594315537879397	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	19635	2.7096138507014498	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	7920	1.0929534859972234	No Hit
GGGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	3542	0.4887930867932027	RNA PCR Primer, Index 38 (95% over 21bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCCGACTATGTCG	2136	0.29476624319319056	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	2058	0.2840023073462482	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG	2020	0.2787583386003019	No Hit
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCTT	1756	0.24232655573372783	RNA PCR Primer, Index 38 (96% over 25bp)
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1544	0.2130707300984486	No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCCGACTATGT	1512	0.20865475641765174	No Hit
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	1418	0.19568283373031098	RNA PCR Primer, Index 27 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	1280	0.1766389472318745	RNA PCR Primer, Index 38 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT	1037	0.1431051470933233	RNA PCR Primer, Index 27 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	975	0.1345491980867794	RNA PCR Primer, Index 38 (95% over 23bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCC	939	0.12958122769588293	No Hit
GGGGTTGGGGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCG	913	0.1259932490802355	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCT	900	0.12419925977241175	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTC	880	0.12143927622191371	No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	863	0.1190932902039904	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT	852	0.11757529925121646	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGTCTCTTATACACATCTG	833	0.11495331487824333	No Hit
GGGGTTGGGGATTTAGCTCAGTGGCTGTCTCTTATACACATCTGACGCCGAC	799	0.11026134284239665	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGCTGTCTCTTATACACATCTGACGCC	760	0.10487937491892547	No Hit
GGGGTTGGGGACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCC	753	0.10391338067625117	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTGTCTCTTATACACATCTGA	734	0.10129139630327803	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.25157250062789627	0.0
2	0.0	0.0	0.0	0.7504395273804169	0.0
3	0.0	0.0	0.0	1.2243287030009302	0.0
4	0.0	0.0	0.0	1.7956452979540243	0.0
5	0.0	0.0	0.0	3.795529378644903	0.0
6	0.0	0.0	0.0	5.245072739366473	0.0
7	0.0	0.0	0.0	6.320638328995559	0.0
8	0.0	0.0	0.0	8.28312463257719	0.0
9	0.0	0.0	0.0	9.367936167100444	0.0
10	0.0	0.0	0.0	10.88661711576199	0.0
11	0.0	0.0	0.0	13.168157517781195	0.0
12	0.0	0.0	0.0	14.555187251083984	0.0
13	0.0	0.0	0.0	15.233591207796401	0.0
14	0.0	0.0	0.0	16.033434440730733	0.0
15	0.0	0.0	0.0	16.55700332026021	0.0
16	0.0	0.0	0.0	17.199803489171206	0.0
17	0.0	0.0	0.0	17.983224819980073	0.0
18	0.0	0.0	0.0	18.989652821669182	0.0
19	0.0	0.0	0.0	19.53212758851957	0.0
20	0.0	0.0	0.0	20.304646984303975	0.0
21	0.0	0.0	0.0	20.94165118775892	0.0
22	0.0	0.0	0.0	21.66173089608386	0.0
23	0.0	0.0	0.0	22.319020978634967	0.0
24	0.0	0.0	0.0	22.795670137805978	0.0
25	0.0	0.0	0.0	23.32972695482735	0.0
26	0.0	0.0	0.0	23.78981621269537	0.0
27	0.0	0.0	0.0	24.598629392168824	0.0
28	0.0	0.0	0.0	25.141932154084362	0.0
29	0.0	0.0	0.0	25.853731911757805	0.0
30	0.0	0.0	0.0	27.461836327455487	0.0
31	0.0	0.0	0.0	28.081314635364773	0.0
32	0.0	0.0	0.0	28.589703605366513	0.0
33	0.0	0.0	0.0	29.1343863590573	0.0
34	0.0	0.0	0.0	29.757866643114806	0.0
35	0.0	0.0	0.0	30.3308392281982	0.0
36	0.0	0.0	0.0	30.750908724584	0.0
37	0.0	0.0	0.0	31.297109469227564	0.0
38	0.0	0.0	0.0	31.922107744237845	0.0
39	0.0	0.0	0.0	34.156728425898585	0.0
40	0.0	0.0	0.0	34.73577297479307	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	30	1.8604442E-6	46.0	1
CGGGTAG	20	6.3099037E-4	46.0	6
CGTATGG	40	5.6061253E-9	46.0	2
CATGCGA	30	1.8604442E-6	46.0	11
ACTTGCG	30	1.8604442E-6	46.0	1
ACCGCGG	25	3.415897E-5	46.0	2
GACGTAG	30	1.8604442E-6	46.0	1
CGATTGA	20	6.3099037E-4	46.0	10
CGCTCGA	20	6.3099037E-4	46.0	44
TACGAAT	45	3.092282E-10	46.0	12
GTCCGGA	25	3.415897E-5	46.0	12
CGTAAGG	115	0.0	46.0	2
TATAGCG	40	5.6061253E-9	46.0	1
ATGCGAA	30	1.8604442E-6	46.0	12
TAATGCG	25	3.415897E-5	46.0	1
TACGTAT	20	6.3099037E-4	46.0	15
CTTACGG	20	6.3099037E-4	46.0	2
CGTTTTT	4715	0.0	45.26829	1
TACGGGA	125	0.0	44.159996	4
AATGCGG	85	0.0	43.294117	2
>>END_MODULE