FastQCFastQC Report
Sat 14 Jan 2017
SRR2933073.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933073.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1573357
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490113.1150590743232462No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC326772.0768967246467267No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG176011.1186907993545012No Hit
GGGGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT58250.37022748174762626No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC55800.3546556820861381No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC54130.34404143496994005No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTT53900.3425795925527391Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT49290.3132791858427553No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT49010.3114995515957281No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA42050.26726292888390873No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT41940.2665637868582909No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT40690.25861899111263364No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCG31330.19912836056915245No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCCTTTACCTTCG26660.1694466036633771No Hit
CGTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG24580.1562264635426035No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG23800.15126891099731338No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCCTTTACCTT21570.13709539538706092No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCT21380.13588778643372101No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTGTCTCTTATACACATCTGA19920.12660826500279337No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGTCTCTTATACACATCTG17630.11205339919674938No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACGG1100.046.0000042
ACGCGAG800.046.01
CTATGCG301.8618448E-646.01
CAGCGCG405.6134013E-946.01
GATACCG206.312169E-446.01
AGTCCCC206.312169E-446.046
ACAGTCG206.312169E-446.043
CGTATCG206.312169E-446.026
ACGCATG206.312169E-446.01
CCGATCT301.8618448E-646.012
CGTGCGG1550.046.02
ACGCACG253.417734E-546.01
CGGCCGA206.312169E-446.028
GACGTTA206.312169E-446.09
GGCCGCG206.312169E-446.01
CGACAGG900.046.02
TACGGCA253.417734E-546.039
TAACGCA206.312169E-446.037
TCGATAT206.312169E-446.023
TCGATAG501.6370905E-1146.01
Produced by FastQC (version 0.11.5)