##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933073.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1573357
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31951299037663	33.0	31.0	34.0	31.0	34.0
2	32.66665162452006	34.0	31.0	34.0	31.0	34.0
3	32.65147388672755	34.0	31.0	34.0	31.0	34.0
4	36.174535722026214	37.0	35.0	37.0	35.0	37.0
5	36.00291287991219	37.0	35.0	37.0	35.0	37.0
6	36.11520144506301	37.0	35.0	37.0	35.0	37.0
7	36.448006396513954	37.0	37.0	37.0	35.0	37.0
8	36.3855711068753	37.0	37.0	37.0	35.0	37.0
9	38.20581978533797	39.0	39.0	39.0	37.0	39.0
10	37.898424197432625	39.0	38.0	39.0	35.0	39.0
11	37.57193504080765	39.0	37.0	39.0	35.0	39.0
12	37.25786391772497	39.0	35.0	39.0	35.0	39.0
13	37.19025497709674	39.0	37.0	39.0	34.0	39.0
14	37.762227517340314	40.0	37.0	41.0	31.0	41.0
15	38.14196841530562	40.0	37.0	41.0	33.0	41.0
16	38.44230012641759	40.0	37.0	41.0	33.0	41.0
17	38.53680823868963	40.0	37.0	41.0	35.0	41.0
18	38.480668405199836	40.0	37.0	41.0	35.0	41.0
19	38.40328482346982	40.0	37.0	41.0	35.0	41.0
20	38.152833082383715	40.0	35.0	41.0	33.0	41.0
21	38.11595334053238	40.0	35.0	41.0	34.0	41.0
22	38.08974949741222	40.0	35.0	41.0	34.0	41.0
23	38.09871758284992	40.0	35.0	41.0	34.0	41.0
24	38.039957873515036	40.0	35.0	41.0	34.0	41.0
25	37.84032422393646	40.0	35.0	41.0	34.0	41.0
26	37.76325271378333	40.0	35.0	41.0	34.0	41.0
27	37.65860894889081	39.0	35.0	41.0	34.0	41.0
28	37.60816140265687	39.0	35.0	41.0	34.0	41.0
29	37.54903559713403	39.0	35.0	41.0	34.0	41.0
30	37.52040763793595	40.0	35.0	41.0	34.0	41.0
31	37.384540190179344	40.0	35.0	41.0	33.0	41.0
32	37.30134165354716	40.0	35.0	41.0	33.0	41.0
33	37.21189024487132	39.0	35.0	41.0	33.0	41.0
34	37.0422205513434	39.0	35.0	41.0	33.0	41.0
35	36.97959585777417	39.0	35.0	41.0	33.0	41.0
36	36.89275924027414	39.0	35.0	41.0	33.0	41.0
37	36.79851870872282	39.0	35.0	41.0	32.0	41.0
38	36.777313731085826	39.0	35.0	41.0	32.0	41.0
39	36.761767990354386	39.0	35.0	41.0	32.0	41.0
40	36.684821054598544	39.0	35.0	41.0	32.0	41.0
41	36.51194992617696	39.0	35.0	41.0	31.0	41.0
42	36.56322627350309	39.0	35.0	41.0	32.0	41.0
43	36.54842734357174	39.0	35.0	41.0	32.0	41.0
44	36.537062472153494	39.0	35.0	41.0	32.0	41.0
45	36.49637494859717	39.0	35.0	41.0	32.0	41.0
46	36.46227906317511	38.0	35.0	41.0	32.0	41.0
47	36.37226897646243	38.0	35.0	41.0	32.0	41.0
48	36.25502412993364	38.0	35.0	41.0	31.0	41.0
49	36.24398467734913	38.0	35.0	41.0	31.0	41.0
50	36.13343316234014	38.0	35.0	41.0	31.0	41.0
51	36.08590230952034	38.0	35.0	41.0	31.0	41.0
52	35.85032322607012	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	2.0
14	11.0
15	42.0
16	73.0
17	170.0
18	325.0
19	608.0
20	984.0
21	1595.0
22	2508.0
23	4022.0
24	6322.0
25	10366.0
26	13321.0
27	14664.0
28	15968.0
29	18270.0
30	22113.0
31	27527.0
32	35403.0
33	49438.0
34	119991.0
35	153698.0
36	138969.0
37	132238.0
38	169031.0
39	629003.0
40	6690.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.233099036010266	23.053954061284248	25.53228542536754	15.180661477337948
2	39.0269341287451	22.5478387931029	25.395825613640135	13.029401464511869
3	31.46304367031767	22.746268011646436	32.65959346797961	13.131094850056279
4	28.474338627533356	26.15903447215095	31.56988528350527	13.796741616810426
5	22.524957781355408	30.0347600703464	32.72505858492383	14.715223563374364
6	23.620386218766626	36.21352305929296	29.536780273008606	10.629310448931806
7	82.29619851057325	1.1900668443334856	12.345577005091661	4.168157640001602
8	79.10728461499838	1.3998730103848014	13.275245224065484	6.217597150551337
9	73.65321411478767	3.1549101697834634	16.575386260079565	6.616489455349295
10	44.013405730549394	25.67465616512972	19.351806360539918	10.960131743780972
11	26.44657251977777	21.939394555717488	34.420541555413045	17.193491369091692
12	26.651166899819938	16.845572873797874	36.60224602553648	19.901014200845708
13	26.415683153918657	18.26953450488351	40.404752386139954	14.91002995505788
14	13.936570021933992	23.01289535687069	44.232173626201806	18.81836099499351
15	10.525201845480714	30.51176560691566	42.92426957136873	16.038762976234892
16	24.551198488327824	28.157881523392337	33.50027997460208	13.790640013677761
17	20.937460474641163	27.70877810948183	28.774715465085166	22.579045950791844
18	18.169874987049983	27.454989554182553	36.6993632087314	17.67577225003607
19	18.070978169608043	31.114870941559992	28.48088513922778	22.333265749604188
20	12.119690572451134	42.76804310782613	25.931368405263395	19.180897914459337
21	17.31526919828113	32.18500314931703	34.975406090289745	15.524321562112094
22	13.388124882019783	27.6905368584498	37.941992821718145	20.97934543781227
23	15.335871007025107	29.216191875079843	35.28436330724686	20.163573810648185
24	18.183540035732513	32.522498072592555	30.482973667133393	18.81098822454154
25	20.942672260650316	28.163601776329212	30.905954592632185	19.987771370388284
26	13.852100953566165	33.04755373383155	27.61788964615151	25.482455666450782
27	20.776212900187307	28.185020945659506	32.09767395448077	18.941092199672422
28	15.020875745301288	31.80848338933885	26.87965922546504	26.290981639894824
29	18.552432791794867	31.23474201977047	26.155284528558997	24.057540659875666
30	22.854444350519305	25.072821997804695	27.71824830601065	24.35448534566535
31	17.802380515038863	26.879977017294866	33.46646692390856	21.851175543757712
32	21.47605406783076	24.719628158135755	29.10242240000203	24.701895374031448
33	19.986182411239152	26.29937134420224	34.47691782602423	19.23752841853438
34	12.320090100339593	25.967342440399733	39.05292950042489	22.659637958835788
35	13.868181220155376	30.622357163695206	31.073367328584677	24.436094287564742
36	18.757535638764754	26.945696367702944	29.778810530604304	24.517957462927995
37	12.271277275278274	30.44356748023494	33.126429665994436	24.15872557849236
38	16.68731254254438	26.04132437838329	34.4226389814899	22.84872409758243
39	14.078877203330203	29.46203563463346	30.558163214070294	25.900923947966035
40	21.815328625353306	25.123223782015142	31.870961263082698	21.190486329548857
41	15.396442129789998	25.74361699220202	28.765181710190372	30.094759167817603
42	15.190068115500804	28.304192881844365	34.97902891714976	21.52671008550507
43	15.508686204084643	28.787554255010146	35.723678732798724	19.980080808106486
44	21.433342845902107	27.096901720334294	29.245047373228072	22.22470806053553
45	16.614347538416265	26.28329107761303	27.53195873536648	29.570402648604226
46	20.183658254293206	25.13002452717343	29.49756476120804	25.188752457325325
47	15.422564618201717	25.078033783813847	33.09344287405846	26.405958723925977
48	14.717003197621391	31.11645990070912	30.42449997044536	23.742036931224128
49	21.586645624610306	25.28726792457147	30.79263002611613	22.333456424702085
50	16.668435707852698	26.289392680745692	31.323787290487793	25.718384320913817
51	15.909930168423314	26.060519004904798	29.085897224851067	28.943653601820817
52	15.532647708053544	27.30696211984947	32.43999931356964	24.72039085852734
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1092.0
1	1839.0
2	2586.0
3	27335.0
4	52084.0
5	28187.5
6	4291.0
7	5501.5
8	6712.0
9	11149.0
10	15586.0
11	19904.0
12	24222.0
13	25390.0
14	25109.0
15	23660.0
16	20742.5
17	17825.0
18	15781.5
19	13738.0
20	12713.0
21	11688.0
22	10943.5
23	10199.0
24	10306.0
25	10413.0
26	11024.0
27	11635.0
28	12472.0
29	13309.0
30	14634.5
31	15960.0
32	17933.5
33	19907.0
34	22236.5
35	24566.0
36	26423.0
37	28280.0
38	32731.5
39	42193.5
40	47204.0
41	60197.5
42	73191.0
43	99702.0
44	126213.0
45	151665.5
46	177118.0
47	180818.5
48	184519.0
49	181242.0
50	177965.0
51	139846.5
52	101728.0
53	88632.5
54	75537.0
55	67713.0
56	59889.0
57	56440.0
58	52991.0
59	61694.0
60	70397.0
61	46315.5
62	22234.0
63	17009.5
64	9828.0
65	7871.0
66	6484.0
67	5097.0
68	4267.5
69	3438.0
70	2739.5
71	2041.0
72	1629.0
73	1217.0
74	952.5
75	688.0
76	512.5
77	337.0
78	272.5
79	208.0
80	164.0
81	120.0
82	89.5
83	59.0
84	30.5
85	2.0
86	7.0
87	12.0
88	6.5
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1573357.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.711987509538275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.39133023093075	16.159497358988535
2	12.804116107946223	6.328303146604898
3	6.000420972244623	4.448469843542418
4	3.605179962029137	3.5636464876520684
5	2.311643819482163	2.8562656596772285
6	1.589859560376503	2.357315375876649
7	1.1865945017870896	2.0526175954934547
8	0.8559379065337183	1.6921541484161269
9	0.6351822361969837	1.4126953938462405
>10	4.654352549182114	23.66012812926521
>50	0.6473824549190234	11.17047871041808
>100	0.3023055388892899	11.555987301203947
>500	0.0069465951807059995	1.2813776849936225
>1k	0.0069465951807059995	3.7363661017177834
>5k	0.0010291252119564445	1.4119696153785037
>10k+	7.718439089673332E-4	6.312727446925264
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49011	3.1150590743232462	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	32677	2.0768967246467267	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	17601	1.1186907993545012	No Hit
GGGGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT	5825	0.37022748174762626	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGC	5580	0.3546556820861381	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTC	5413	0.34404143496994005	No Hit
CTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCTT	5390	0.3425795925527391	Illumina Single End Adapter 1 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCT	4929	0.3132791858427553	No Hit
CCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	4901	0.3114995515957281	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	4205	0.26726292888390873	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTT	4194	0.2665637868582909	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTGCT	4069	0.25861899111263364	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCG	3133	0.19912836056915245	No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCCTTTACCTTCG	2666	0.1694466036633771	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCTTCTG	2458	0.1562264635426035	No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2380	0.15126891099731338	No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCCTTTACCTT	2157	0.13709539538706092	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTTTACCTTCGTATGCCGTCT	2138	0.13588778643372101	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTGTCTCTTATACACATCTGA	1992	0.12660826500279337	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGTCTCTTATACACATCTG	1763	0.11205339919674938	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.355836596525772E-5	0.0	0.0	0.3505879466643616	0.0
2	6.355836596525772E-5	0.0	0.0	1.1073774102126854	0.0
3	6.355836596525772E-5	0.0	0.0	1.9127890237244312	0.0
4	6.355836596525772E-5	0.0	0.0	2.784809804767767	0.0
5	6.355836596525772E-5	0.0	0.0	5.497862214360759	0.0
6	6.355836596525772E-5	0.0	0.0	8.594807154383906	0.0
7	6.355836596525772E-5	0.0	0.0	11.196886657001558	0.0
8	6.355836596525772E-5	0.0	0.0	15.374832285361808	0.0
9	6.355836596525772E-5	0.0	0.0	17.625815374387376	0.0
10	6.355836596525772E-5	0.0	0.0	19.750571548605944	0.0
11	6.355836596525772E-5	0.0	0.0	22.484661777333436	0.0
12	6.355836596525772E-5	0.0	0.0	24.113217788461235	0.0
13	1.2711673193051544E-4	0.0	0.0	24.869498785081834	0.0
14	1.2711673193051544E-4	0.0	0.0	25.563492583056483	0.0
15	1.2711673193051544E-4	0.0	0.0	26.06128170529638	0.0
16	1.9067509789577318E-4	0.0	0.0	26.755275503271033	0.0
17	1.9067509789577318E-4	0.0	0.0	27.620749772619945	0.0
18	1.9067509789577318E-4	0.0	0.0	28.59611645672279	0.0
19	1.9067509789577318E-4	0.0	0.0	29.191531229085324	0.0
20	1.9067509789577318E-4	0.0	0.0	29.974633856143267	0.0
21	1.9067509789577318E-4	0.0	0.0	30.65960236615085	0.0
22	2.542334638610309E-4	0.0	0.0	31.431518720798902	0.0
23	2.542334638610309E-4	0.0	0.0	32.15227059084493	0.0
24	2.542334638610309E-4	0.0	0.0	32.66232647771612	0.0
25	2.542334638610309E-4	0.0	0.0	33.182678819873686	0.0
26	2.542334638610309E-4	0.0	0.0	33.64258715599829	0.0
27	2.542334638610309E-4	0.0	0.0	34.25147630194546	0.0
28	2.542334638610309E-4	0.0	0.0	34.737570684847746	0.0
29	2.542334638610309E-4	0.0	0.0	35.369023050712585	0.0
30	3.177918298262886E-4	0.0	0.0	37.02382866698404	0.0
31	3.177918298262886E-4	0.0	0.0	37.709941227578994	0.0
32	3.177918298262886E-4	0.0	0.0	38.24643739469173	0.0
33	3.177918298262886E-4	0.0	0.0	38.792340199967335	0.0
34	3.177918298262886E-4	0.0	0.0	39.358391007253914	0.0
35	3.177918298262886E-4	0.0	0.0	39.94325509086622	0.0
36	3.177918298262886E-4	0.0	0.0	40.38110867400088	0.0
37	3.177918298262886E-4	0.0	0.0	40.9094058119041	0.0
38	3.177918298262886E-4	0.0	0.0	41.510286603739644	0.0
39	3.177918298262886E-4	0.0	0.0	43.62862338299572	0.0
40	3.177918298262886E-4	0.0	0.0	44.21450440046347	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTACGG	110	0.0	46.000004	2
ACGCGAG	80	0.0	46.0	1
CTATGCG	30	1.8618448E-6	46.0	1
CAGCGCG	40	5.6134013E-9	46.0	1
GATACCG	20	6.312169E-4	46.0	1
AGTCCCC	20	6.312169E-4	46.0	46
ACAGTCG	20	6.312169E-4	46.0	43
CGTATCG	20	6.312169E-4	46.0	26
ACGCATG	20	6.312169E-4	46.0	1
CCGATCT	30	1.8618448E-6	46.0	12
CGTGCGG	155	0.0	46.0	2
ACGCACG	25	3.417734E-5	46.0	1
CGGCCGA	20	6.312169E-4	46.0	28
GACGTTA	20	6.312169E-4	46.0	9
GGCCGCG	20	6.312169E-4	46.0	1
CGACAGG	90	0.0	46.0	2
TACGGCA	25	3.417734E-5	46.0	39
TAACGCA	20	6.312169E-4	46.0	37
TCGATAT	20	6.312169E-4	46.0	23
TCGATAG	50	1.6370905E-11	46.0	1
>>END_MODULE