FastQCFastQC Report
Sat 14 Jan 2017
SRR2933072.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933072.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1529512
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC305771.9991343644247317No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG169781.110027250521735No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158161.0340553065291413No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA39480.2581215446495353No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCTT27450.17946900710814953Illumina Single End Adapter 1 (95% over 21bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCCACTTCCGTCG26970.1763307512461491No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG26090.170577282165815No Hit
GGGGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCT25980.16985809853077322No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT23390.15292459294206256No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCCACTTCCGT22010.1439021073388113No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT20210.13213364785630974No Hit
GCCCTGCTAGAGGACTTTCGTCTGTATCTATGCTCTGCCCCATGCAGCTCCT19230.12572637547139218No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17390.1136963946670572No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG16560.1082698272390148No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG750.046.0000041
CACGTGA1550.046.00000443
ATTAGCG206.312114E-446.01
GTCGAAC253.4176897E-546.01
CGCGGAT206.312114E-446.025
AAGCGTT253.4176897E-546.023
TAACGTT206.312114E-446.032
ACGGGTA501.6370905E-1146.05
ATCGTCC206.312114E-446.022
TACGCCC301.8618102E-646.017
CTACGAA1200.046.011
ACAATCG206.312114E-446.018
AACGCAT206.312114E-446.034
AATTCGC301.8618102E-646.010
ATGACGT253.4176897E-546.019
CGTAATC206.312114E-446.017
ACGTATA206.312114E-446.028
ACCGTCA206.312114E-446.046
TAGGCGT253.4176897E-546.032
ATAGTGC301.8618102E-646.019
Produced by FastQC (version 0.11.5)