##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933072.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1529512
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43774354173096	33.0	31.0	34.0	31.0	34.0
2	32.8308944290728	34.0	31.0	34.0	31.0	34.0
3	32.8867828431552	34.0	31.0	34.0	31.0	34.0
4	36.34013528497979	37.0	37.0	37.0	35.0	37.0
5	36.17141741941221	37.0	37.0	37.0	35.0	37.0
6	36.262206507696575	37.0	37.0	37.0	35.0	37.0
7	36.48629301371941	37.0	37.0	37.0	35.0	37.0
8	36.43976902436856	37.0	37.0	37.0	35.0	37.0
9	38.265448718284	39.0	39.0	39.0	37.0	39.0
10	37.9631830283123	39.0	38.0	39.0	35.0	39.0
11	37.62688883774694	39.0	37.0	39.0	35.0	39.0
12	37.16532724163001	39.0	35.0	39.0	35.0	39.0
13	37.05405449581304	39.0	35.0	39.0	34.0	39.0
14	37.726498386413446	40.0	36.0	41.0	32.0	41.0
15	38.02762842004508	40.0	36.0	41.0	33.0	41.0
16	38.294227832145154	40.0	36.0	41.0	34.0	41.0
17	38.366350182280364	40.0	36.0	41.0	35.0	41.0
18	38.317681718090476	40.0	36.0	41.0	35.0	41.0
19	38.27465819163236	40.0	36.0	41.0	35.0	41.0
20	38.08466229751712	40.0	35.0	41.0	34.0	41.0
21	38.05494105309406	40.0	35.0	41.0	34.0	41.0
22	37.990298212763285	40.0	35.0	41.0	34.0	41.0
23	37.978376763307516	40.0	35.0	41.0	34.0	41.0
24	37.94214625318402	40.0	35.0	41.0	34.0	41.0
25	37.78096608591498	40.0	35.0	41.0	34.0	41.0
26	37.68557618377626	40.0	35.0	41.0	34.0	41.0
27	37.59710090538682	40.0	35.0	41.0	34.0	41.0
28	37.55429444162583	39.0	35.0	41.0	34.0	41.0
29	37.508400718660596	40.0	35.0	41.0	34.0	41.0
30	37.47332024854986	40.0	35.0	41.0	34.0	41.0
31	37.35083869887912	40.0	35.0	41.0	33.0	41.0
32	37.23375691070093	40.0	35.0	41.0	33.0	41.0
33	37.139039772162626	40.0	35.0	41.0	33.0	41.0
34	36.97359353833118	40.0	35.0	41.0	33.0	41.0
35	36.87687445407424	40.0	35.0	41.0	32.0	41.0
36	36.80539086976761	39.0	35.0	41.0	32.0	41.0
37	36.687312685353234	39.0	35.0	41.0	32.0	41.0
38	36.67529512681169	39.0	35.0	41.0	32.0	41.0
39	36.62805064621919	39.0	35.0	41.0	32.0	41.0
40	36.53990423089194	39.0	35.0	41.0	31.0	41.0
41	36.36697064161641	39.0	35.0	41.0	31.0	41.0
42	36.39667488715355	39.0	35.0	41.0	31.0	41.0
43	36.36076081782948	39.0	35.0	41.0	31.0	41.0
44	36.35008290225902	39.0	35.0	41.0	31.0	41.0
45	36.2897839310839	38.0	35.0	41.0	31.0	41.0
46	36.24928473918479	38.0	35.0	41.0	31.0	41.0
47	36.15403148193672	38.0	35.0	41.0	31.0	41.0
48	36.033053026063214	38.0	35.0	41.0	31.0	41.0
49	36.006400080548566	38.0	35.0	41.0	31.0	41.0
50	35.89901419537735	38.0	35.0	41.0	31.0	41.0
51	35.82408572145887	37.0	35.0	41.0	31.0	41.0
52	35.5694103740278	37.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	4.0
13	6.0
14	14.0
15	37.0
16	93.0
17	190.0
18	355.0
19	667.0
20	1186.0
21	1951.0
22	3067.0
23	4852.0
24	7346.0
25	10983.0
26	14456.0
27	16267.0
28	17627.0
29	18865.0
30	21923.0
31	26475.0
32	32482.0
33	45901.0
34	124776.0
35	179811.0
36	85461.0
37	104456.0
38	176502.0
39	629518.0
40	4240.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.312015858652956	22.302015283306048	25.14802106815769	13.23794778988331
2	38.10725250929708	22.387794276867393	25.85412863710778	13.650824576727741
3	34.33356521557202	23.26866346913264	28.81827667909765	13.579494636197689
4	30.9316304808331	25.57135870787545	28.95145641224129	14.545554399050154
5	26.136506284357363	30.725747820219784	28.75897671937193	14.378769176050923
6	27.822272724895257	36.624165093180046	26.395216251981026	9.15834592994367
7	86.54838929017883	1.9618675760634765	9.195808859296298	2.293934274461397
8	84.77880526599334	2.319759504992442	9.493289362881756	3.4081458661324655
9	78.92210064386549	4.369432864861472	11.885294133030666	4.823172358242368
10	50.29211931648787	18.39148695793168	17.98325217454979	13.333141551030655
11	34.74271532358033	24.536911119363563	25.676947941565675	15.04342561549043
12	34.325392674264734	18.28537468159779	30.55615124301084	16.833081401126634
13	31.280173022506524	20.43089560591875	32.62896923986213	15.659962131712598
14	19.046467108463354	21.291235374420076	39.45997154648018	20.202325970636387
15	15.606219500075841	28.42945985386189	38.422124180784465	17.542196465277815
16	30.225130629900253	24.132533775478716	30.105746146483327	15.536589448137706
17	27.195275355799758	22.54261489939275	26.90766728211351	23.354442462693985
18	23.04924708011444	22.733460084000647	35.23391774631386	18.98337508957105
19	23.85898247284101	24.65145745832658	28.445412654493722	23.044147414338692
20	18.66726119180497	35.914723127376575	27.17095387286925	18.2470618079492
21	24.812685353236848	25.791232759206856	33.84641637332692	15.549665514229375
22	20.44625998357646	22.409500546579565	37.23501352065234	19.909225949191637
23	22.007280753599844	22.415711677973103	35.755064360397306	19.82194320802975
24	23.317437195654563	27.215085595928635	30.225392151222092	19.242085057194714
25	24.53416514548431	23.883500096762887	31.686446395974667	19.895888361778134
26	18.553434036476993	28.894444764081616	27.476214635779257	25.075906563662137
27	23.32194843845619	25.152532310959312	32.079970605003425	19.445548645581074
28	17.47492010523618	29.090978037439392	27.531395634686096	25.902706222638333
29	21.943992593716168	28.187552631166017	27.156374059177047	22.712080715940772
30	24.912782639168572	25.09107480032847	26.66288332487748	23.33325923562548
31	22.166351097604988	24.338547196752952	31.063698748358952	22.431402957283108
32	26.302899225373842	23.05140463101957	26.133498789156278	24.512197354450308
33	25.11173498475331	23.775949453158916	29.827879742035368	21.28443582005241
34	18.948396612775838	22.544576309306496	33.96514705343927	24.541880024478395
35	20.692090026099827	26.89145296015984	28.776890929917514	23.639566083822814
36	24.222824011841684	25.54357206743066	25.908851973701413	24.32475194702624
37	19.29981588898943	28.232599678851816	27.036531913446904	25.43105251871185
38	23.243884323888928	23.972875008499443	31.080174591634457	21.703066075977173
39	20.50582146462401	26.89720642924018	27.456142874328542	25.14082923180727
40	26.901586911380885	22.908940890950838	28.712883586398796	21.476588611269477
41	19.763427812269533	24.0305404599637	26.134741015434987	30.071290712331777
42	19.039340652443393	26.112838604731444	30.33156980788644	24.516250934938725
43	19.036463917903227	26.721267959976775	31.147189430354256	23.09507869176574
44	24.234723231985104	24.077810438885084	27.420379833567832	24.267086495561983
45	19.426980631730906	24.456493312899802	26.58540763328434	29.53111842208495
46	21.736083142858636	23.646169497199107	28.90464409563312	25.71310326430914
47	18.308911600562794	24.21837814937052	31.75032297883246	25.722387271234226
48	18.492238047167987	28.241687544785528	29.953475356845843	23.312599051200642
49	23.762481105084497	24.381698214855458	29.655929472929927	22.19989120713012
50	21.398459116371757	24.5313537912746	28.81513842323565	25.25504866911799
51	20.784145531385175	24.75554294441626	26.92734676158147	27.532964762617095
52	20.353289153664697	26.061057383008436	28.846717122847025	24.73893634047984
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1043.0
1	4078.0
2	7113.0
3	14151.0
4	21189.0
5	13002.5
6	4816.0
7	5661.0
8	6506.0
9	7476.0
10	8446.0
11	9564.5
12	10683.0
13	11057.0
14	11125.0
15	10819.0
16	9800.5
17	8782.0
18	7977.0
19	7172.0
20	6891.5
21	6611.0
22	7078.5
23	7546.0
24	8209.5
25	8873.0
26	10479.0
27	12085.0
28	13639.0
29	15193.0
30	15922.0
31	16651.0
32	20610.0
33	24569.0
34	25856.5
35	27144.0
36	28824.0
37	30504.0
38	35058.0
39	42199.5
40	44787.0
41	50550.5
42	56314.0
43	59771.5
44	63229.0
45	69095.5
46	74962.0
47	91496.0
48	108030.0
49	128742.5
50	149455.0
51	145061.5
52	140668.0
53	136253.0
54	131838.0
55	121726.5
56	111615.0
57	103186.5
58	94758.0
59	100212.0
60	105666.0
61	79299.0
62	52932.0
63	45277.5
64	31766.5
65	25910.0
66	20973.5
67	16037.0
68	12972.0
69	9907.0
70	9050.5
71	8194.0
72	6395.5
73	4597.0
74	3530.0
75	2463.0
76	1873.5
77	1284.0
78	1138.0
79	992.0
80	903.5
81	815.0
82	608.0
83	401.0
84	243.0
85	85.0
86	85.0
87	85.0
88	74.0
89	34.5
90	6.0
91	3.5
92	1.0
93	3.5
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1529512.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.15980963390858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.3043689585663	13.661781353121105
2	11.57075244794572	4.433868284474434
3	4.7521415713614585	2.7315038358200616
4	2.4515671035617426	1.8788623603598251
5	1.463006227127104	1.4015460402489066
6	0.9228391054775624	1.0608852950205945
7	0.6546208060754715	0.8779687018761272
8	0.49505562078063997	0.7588137161882799
9	0.3952948402021302	0.6816396498785214
>10	3.5749252081515337	15.001336213642816
>50	1.2301585702559596	17.165958394345942
>100	1.170192391092402	32.6282039177767
>500	0.0068532497282116295	0.9605104136155375
>1k	0.007195912214622211	2.5965951372860228
>5k	0.0	0.0
>10k+	0.0010279874592317444	4.160526686345197
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	30577	1.9991343644247317	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	16978	1.110027250521735	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15816	1.0340553065291413	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	3948	0.2581215446495353	No Hit
CTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCTT	2745	0.17946900710814953	Illumina Single End Adapter 1 (95% over 21bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCCACTTCCGTCG	2697	0.1763307512461491	No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	2609	0.170577282165815	No Hit
GGGGCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCT	2598	0.16985809853077322	No Hit
GCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT	2339	0.15292459294206256	No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCCACTTCCGT	2201	0.1439021073388113	No Hit
CCTGTCTCTTATACACATCTGACGCCACTTCCGTCGTATGCCGTCTTCTGCT	2021	0.13213364785630974	No Hit
GCCCTGCTAGAGGACTTTCGTCTGTATCTATGCTCTGCCCCATGCAGCTCCT	1923	0.12572637547139218	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1739	0.1136963946670572	No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1656	0.1082698272390148	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1863993221367338	0.0
2	0.0	0.0	0.0	0.5775044589385373	0.0
3	0.0	0.0	0.0	0.8664201392339518	0.0
4	0.0	0.0	0.0	1.238957262185586	0.0
5	0.0	0.0	0.0	2.377294195795783	0.0
6	0.0	0.0	0.0	3.3026873931031595	0.0
7	0.0	0.0	0.0	4.094900857266893	0.0
8	0.0	0.0	0.0	5.530652914132089	0.0
9	0.0	0.0	0.0	6.266770054762564	0.0
10	6.538033045834226E-5	0.0	0.0	7.1131184325458054	0.0
11	6.538033045834226E-5	0.0	0.0	8.485255427875035	0.0
12	6.538033045834226E-5	0.0	0.0	9.280214865918019	0.0
13	1.961409913750268E-4	0.0	0.0	9.64340260161411	0.0
14	1.961409913750268E-4	0.0	0.0	9.83862826836272	0.0
15	1.961409913750268E-4	0.0	0.0	10.093088514506588	0.0
16	1.961409913750268E-4	0.0	0.0	10.526560105445396	0.0
17	1.961409913750268E-4	0.0	0.0	10.97846894957346	0.0
18	1.961409913750268E-4	0.0	0.0	11.552965913310912	0.0
19	1.961409913750268E-4	0.0	0.0	11.8824173984905	0.0
20	1.961409913750268E-4	0.0	0.0	12.386238225002485	0.0
21	1.961409913750268E-4	0.0	0.0	12.826705511300336	0.0
22	1.961409913750268E-4	0.0	0.0	13.283452499882316	0.0
23	1.961409913750268E-4	0.0	0.0	13.677434371224287	0.0
24	1.961409913750268E-4	0.0	0.0	13.981649048846952	0.0
25	1.961409913750268E-4	0.0	0.0	14.354578453781336	0.0
26	1.961409913750268E-4	0.0	0.0	14.664154318501588	0.0
27	1.961409913750268E-4	0.0	0.0	15.112924906767649	0.0
28	1.961409913750268E-4	0.0	0.0	15.459636799188237	0.0
29	1.961409913750268E-4	0.0	0.0	15.919914325614968	0.0
30	1.961409913750268E-4	0.0	0.0	17.38881421002254	0.0
31	1.961409913750268E-4	0.0	0.0	17.904599637008406	0.0
32	1.961409913750268E-4	0.0	0.0	18.234181882848908	0.0
33	1.961409913750268E-4	0.0	0.0	18.61822594396121	0.0
34	2.6152132183336906E-4	0.0	0.0	19.063792896034812	0.0
35	3.2690165229171136E-4	0.0	0.0	19.474839033626413	0.0
36	3.2690165229171136E-4	0.0	0.0	19.776569258691662	0.0
37	3.922819827500536E-4	0.0	0.0	20.14224144694517	0.0
38	3.922819827500536E-4	0.0	0.0	20.616118082107235	0.0
39	3.922819827500536E-4	0.0	0.0	22.333920884569718	0.0
40	3.922819827500536E-4	0.0	0.0	22.72862193954673	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTAAG	75	0.0	46.000004	1
CACGTGA	155	0.0	46.000004	43
ATTAGCG	20	6.312114E-4	46.0	1
GTCGAAC	25	3.4176897E-5	46.0	1
CGCGGAT	20	6.312114E-4	46.0	25
AAGCGTT	25	3.4176897E-5	46.0	23
TAACGTT	20	6.312114E-4	46.0	32
ACGGGTA	50	1.6370905E-11	46.0	5
ATCGTCC	20	6.312114E-4	46.0	22
TACGCCC	30	1.8618102E-6	46.0	17
CTACGAA	120	0.0	46.0	11
ACAATCG	20	6.312114E-4	46.0	18
AACGCAT	20	6.312114E-4	46.0	34
AATTCGC	30	1.8618102E-6	46.0	10
ATGACGT	25	3.4176897E-5	46.0	19
CGTAATC	20	6.312114E-4	46.0	17
ACGTATA	20	6.312114E-4	46.0	28
ACCGTCA	20	6.312114E-4	46.0	46
TAGGCGT	25	3.4176897E-5	46.0	32
ATAGTGC	30	1.8618102E-6	46.0	19
>>END_MODULE