FastQCFastQC Report
Sat 14 Jan 2017
SRR2933071.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933071.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815418
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC147141.804473288546488No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86751.0638715358257973No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG80790.9907801888111374No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA15460.18959601088030922No Hit
GGGGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT15250.187020644626437No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCTTCACCCGTCG12210.1497391521894292No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCTT11200.1373528668731865Illumina Single End Adapter 2 (95% over 21bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11150.13673968443178836No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG11020.13514541008415315No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCTTCACCCGT9660.11846684767812336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGG1050.046.0000042
AACGTAT206.3103717E-446.015
AATCCCG206.3103717E-446.014
TCGTTAG453.092282E-1046.01
AAATCCG206.3103717E-446.038
TTGTGCG551.8189894E-1246.01
ATATGCG206.3103717E-446.01
TACGGGT453.092282E-1046.04
TATGCGT301.8607334E-646.011
TCGTAAT206.3103717E-446.043
TCGTAAG301.8607334E-646.01
ACGTATC206.3103717E-446.016
ATACGGA253.4162764E-546.04
TATCGTC206.3103717E-446.019
CGTTAGG1250.044.162
CGTTTTT30600.042.9934651
GCAAGGC17850.042.13445346
CGAATAT1100.041.8181814
GGGCGAT4900.041.7755137
CTAGACG1500.041.39999817
Produced by FastQC (version 0.11.5)