##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933071.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	815418
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51838198322823	33.0	31.0	34.0	31.0	34.0
2	32.913200346325446	34.0	31.0	34.0	31.0	34.0
3	32.95646895212026	34.0	31.0	34.0	31.0	34.0
4	36.387830535995036	37.0	37.0	37.0	35.0	37.0
5	36.28282794836513	37.0	37.0	37.0	35.0	37.0
6	36.38783666781945	37.0	37.0	37.0	35.0	37.0
7	36.535135353892116	37.0	37.0	37.0	35.0	37.0
8	36.483247855701	37.0	37.0	37.0	35.0	37.0
9	38.30551447233198	39.0	39.0	39.0	37.0	39.0
10	38.01927109776826	39.0	39.0	39.0	35.0	39.0
11	37.70439946138055	39.0	37.0	39.0	35.0	39.0
12	37.23060197346637	39.0	35.0	39.0	35.0	39.0
13	37.11190824828493	39.0	35.0	39.0	34.0	39.0
14	38.01399404967759	40.0	36.0	41.0	33.0	41.0
15	38.26018925262871	40.0	36.0	41.0	33.0	41.0
16	38.413239590001695	40.0	36.0	41.0	34.0	41.0
17	38.42541494055809	40.0	36.0	41.0	35.0	41.0
18	38.35923293329311	40.0	36.0	41.0	35.0	41.0
19	38.28227485780299	40.0	36.0	41.0	35.0	41.0
20	38.104310917836	40.0	35.0	41.0	34.0	41.0
21	38.059256724771835	40.0	35.0	41.0	34.0	41.0
22	37.99512274686112	40.0	35.0	41.0	34.0	41.0
23	37.97637040143828	40.0	35.0	41.0	34.0	41.0
24	37.952712596484254	40.0	35.0	41.0	34.0	41.0
25	37.80017487963229	40.0	35.0	41.0	34.0	41.0
26	37.69526917482813	40.0	35.0	41.0	34.0	41.0
27	37.59846483643972	40.0	35.0	41.0	34.0	41.0
28	37.58123563620131	40.0	35.0	41.0	34.0	41.0
29	37.54102681078907	40.0	35.0	41.0	34.0	41.0
30	37.482421285769995	40.0	35.0	41.0	34.0	41.0
31	37.34358329102375	40.0	35.0	41.0	33.0	41.0
32	37.19271588314214	40.0	35.0	41.0	33.0	41.0
33	37.045500589881506	40.0	35.0	41.0	33.0	41.0
34	36.87190864072169	40.0	35.0	41.0	32.0	41.0
35	36.74125172610857	40.0	35.0	41.0	32.0	41.0
36	36.63910043683117	39.0	35.0	41.0	32.0	41.0
37	36.510877856510405	39.0	35.0	41.0	31.0	41.0
38	36.4437490955559	39.0	35.0	41.0	31.0	41.0
39	36.39910205563282	39.0	35.0	41.0	31.0	41.0
40	36.293502228304995	39.0	35.0	41.0	31.0	41.0
41	36.13681939814917	39.0	35.0	41.0	31.0	41.0
42	36.156952630430034	39.0	35.0	41.0	31.0	41.0
43	36.11678182232916	38.0	35.0	41.0	31.0	41.0
44	36.08066782926058	38.0	35.0	41.0	31.0	41.0
45	36.027416613319794	38.0	35.0	41.0	31.0	41.0
46	35.96702059557184	38.0	35.0	41.0	31.0	41.0
47	35.884893392100736	38.0	35.0	41.0	31.0	41.0
48	35.76644248716609	37.0	35.0	41.0	30.0	41.0
49	35.73167994819835	37.0	35.0	41.0	30.0	41.0
50	35.606263290729416	37.0	35.0	40.0	30.0	41.0
51	35.54167678417695	37.0	35.0	40.0	30.0	41.0
52	35.29109487404006	36.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	3.0
13	4.0
14	10.0
15	16.0
16	52.0
17	88.0
18	190.0
19	370.0
20	608.0
21	1015.0
22	1596.0
23	2720.0
24	4241.0
25	6684.0
26	8952.0
27	10107.0
28	10264.0
29	10240.0
30	11223.0
31	13169.0
32	16675.0
33	23829.0
34	62955.0
35	98312.0
36	44683.0
37	58611.0
38	98726.0
39	328070.0
40	2002.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.473965499903116	21.439188244556778	24.498968627133568	13.587877628406536
2	40.440853648067616	22.46958001908224	24.09647567259982	12.993090660250326
3	35.43127573833298	22.86029987074114	28.26685209303695	13.441572297888934
4	31.292907441336837	25.89849623138071	28.62360163744239	14.184994689840059
5	26.447417152920345	30.230016997417273	28.810131736115707	14.512434113546671
6	29.01898167565592	36.94877964430513	26.104893441155337	7.927345238883615
7	86.64574488176616	2.1560721004441894	9.462018253214916	1.7361647645747336
8	85.25185855597988	2.513066917826195	9.807730513675194	2.4273440125187324
9	79.62394747233934	4.344397597305922	11.951538965291421	4.0801159650633165
10	50.85391786789108	20.271075693693298	17.4389086333635	11.436097805052132
11	35.40687107716533	24.961676097412617	24.692856915103665	14.938595910318389
12	34.07614254284306	18.74829841872512	30.256506478885676	16.919052559546145
13	31.67038255226154	19.600131466315435	32.94420775602207	15.785278225400958
14	21.490082387192828	20.147458113507426	38.90360526748245	19.458854231817302
15	18.923791233453272	26.24200103505196	37.097537704588326	17.736670026906445
16	32.14523103488027	22.481230485468803	29.1925123065716	16.18102617307933
17	28.962691527535572	21.327711676710596	27.72075181072775	21.988844985026084
18	26.2225018334155	21.62547307025354	33.22467740471758	18.92734769161338
19	26.399221013026448	23.019482032528103	28.577367681360972	22.00392927308448
20	21.74725110311521	34.00673029047679	26.554601443676738	17.691417162731263
21	27.227385218378796	23.82017566450581	33.486015761241475	15.466423355873921
22	23.709312279101024	20.479312450792108	36.56958762254451	19.241787647562354
23	24.11352214447069	21.636510354198705	34.98402046557717	19.26594703575344
24	24.87374573531612	26.077668140757254	29.74707450657209	19.301511617354535
25	26.09925216269447	22.744408389316888	31.533520231341473	19.622819216647166
26	20.46545452761651	26.93465192085532	28.175610545756903	24.424283005771272
27	24.30777834190562	23.071357267070386	32.281480173358936	20.33938421766505
28	19.347009754506278	27.47474301523881	28.81356555778754	24.364681672467373
29	23.27260374433726	26.57851555889127	28.073086441555127	22.075794255216344
30	26.090177062561775	22.450816636375453	28.73716302558933	22.72184327547344
31	24.24989391943764	22.805603996968426	31.873223303876046	21.071278779717886
32	27.193537547613616	22.717183088918812	26.995479619042012	23.09379974442556
33	25.592518193123038	22.163847253801116	30.433103022008346	21.8105315310675
34	20.41885266207025	22.995690553801854	33.489817492378144	23.09563929174975
35	22.597612512846172	27.044779487330423	28.336877527844617	22.020730471978787
36	25.981030587992908	24.425632007142347	25.868818201217042	23.724519203647702
37	22.281087736596444	26.82403380842709	27.080711978396355	23.81416647658011
38	24.332183003073272	24.300788062073686	29.37180685243642	21.99522208241663
39	22.267720359373968	26.527130870302102	25.58013190780679	25.625016862517136
40	27.340946606525733	23.421852350573573	27.569418384190687	21.66778265871001
41	21.405831119744718	23.820298300994093	26.60696722417214	28.166903355089048
42	20.442153594843383	25.40095999843025	30.624906489677688	23.53197991704868
43	20.646343347828967	25.505691559421056	30.663169074020928	23.184796018729045
44	24.739826690114764	23.735679124081148	28.03678604102436	23.48770814477973
45	20.689511391703398	23.29921586229394	26.93734992359747	29.073922822405194
46	22.872195610104264	23.483661140666502	28.729436926827713	24.914706322401518
47	19.639743052029758	23.084356734828027	32.2016438194889	25.074256393653314
48	20.60857130943884	28.012136106880153	29.090478748322944	22.288813835358063
49	25.18990260210101	24.702667834166032	28.920995121520498	21.18643444221246
50	22.855762320674796	24.720450124966582	27.44555553102826	24.978232023330367
51	22.12975431005938	25.57240580904518	26.612976412097844	25.684863468797598
52	22.676222501833415	25.755992631999785	27.500619314265812	24.067165551900988
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	450.0
1	2125.0
2	3800.0
3	8049.5
4	12299.0
5	7810.5
6	3322.0
7	3386.0
8	3450.0
9	3785.5
10	4121.0
11	4504.0
12	4887.0
13	5148.0
14	5103.0
15	4797.0
16	4564.0
17	4331.0
18	4132.0
19	3933.0
20	3879.0
21	3825.0
22	4003.5
23	4182.0
24	4310.5
25	4439.0
26	5568.0
27	6697.0
28	6810.0
29	6923.0
30	7970.5
31	9018.0
32	10142.5
33	11267.0
34	12709.5
35	14152.0
36	15603.5
37	17055.0
38	18407.0
39	20895.5
40	22032.0
41	25389.0
42	28746.0
43	30113.0
44	31480.0
45	34073.5
46	36667.0
47	41718.0
48	46769.0
49	58031.5
50	69294.0
51	68645.0
52	67996.0
53	69781.5
54	71567.0
55	66851.0
56	62135.0
57	59355.0
58	56575.0
59	57643.5
60	58712.0
61	45676.0
62	32640.0
63	28413.5
64	21221.0
65	18255.0
66	16194.0
67	14133.0
68	11745.0
69	9357.0
70	7639.0
71	5921.0
72	5104.0
73	4287.0
74	3490.0
75	2693.0
76	2167.0
77	1641.0
78	1330.0
79	1019.0
80	853.5
81	688.0
82	507.0
83	326.0
84	227.0
85	128.0
86	82.0
87	36.0
88	21.0
89	7.5
90	9.0
91	5.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	815418.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.72090693611258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.00864192966837	18.295607496114112
2	8.831956032570947	4.3666792629004885
3	3.208338608814862	2.3793912050414736
4	1.6718227732958821	1.6531590076928455
5	1.0596773321349553	1.3098092355008146
6	0.7740579560257737	1.1481248816442404
7	0.6137852302543234	1.0621329289114292
8	0.5277714287227372	1.0437590698395167
9	0.4457468152598296	0.9917338983396133
>10	8.122704990602479	49.67314552042091
>50	0.678191927172651	9.974683407827936
>100	0.04634228451635964	2.181858057855494
>500	0.006477953749598659	1.1037403002701685
>1k	0.0029898248075070732	0.9397806641474459
>5k	9.966082691690245E-4	2.063846539143572
>10k+	4.983041345845122E-4	1.8125485243499173
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	14714	1.804473288546488	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8675	1.0638715358257973	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	8079	0.9907801888111374	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	1546	0.18959601088030922	No Hit
GGGGCTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCT	1525	0.187020644626437	No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCTTCACCCGTCG	1221	0.1497391521894292	No Hit
CTGTCTCTTATACACATCTGACGCTTCACCCGTCGTATGCCGTCTTCTGCTT	1120	0.1373528668731865	Illumina Single End Adapter 2 (95% over 21bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1115	0.13673968443178836	No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1102	0.13514541008415315	No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCTTCACCCGT	966	0.11846684767812336	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.226364882796308E-4	0.0	0.0	0.14029614259189765	0.0
2	2.452729765592616E-4	0.0	0.0	0.3117419532068215	0.0
3	2.452729765592616E-4	0.0	0.0	0.48551785709905837	0.0
4	2.452729765592616E-4	0.0	0.0	0.6948583425923882	0.0
5	2.452729765592616E-4	0.0	0.0	1.4113007071219914	0.0
6	2.452729765592616E-4	0.0	0.0	1.9501654366226893	0.0
7	2.452729765592616E-4	0.0	0.0	2.3546205749689118	0.0
8	2.452729765592616E-4	0.0	0.0	3.145993833837369	0.0
9	2.452729765592616E-4	0.0	0.0	3.573259359003603	0.0
10	2.452729765592616E-4	0.0	0.0	4.205205183108541	0.0
11	2.452729765592616E-4	0.0	0.0	5.231059407567653	0.0
12	2.452729765592616E-4	0.0	0.0	5.898569813273683	0.0
13	2.452729765592616E-4	0.0	0.0	6.228216693769331	0.0
14	2.452729765592616E-4	0.0	0.0	6.529902454937222	0.0
15	2.452729765592616E-4	0.0	0.0	6.800070638617249	0.0
16	2.452729765592616E-4	0.0	0.0	7.154244816768823	0.0
17	2.452729765592616E-4	0.0	0.0	7.518475186959327	0.0
18	2.452729765592616E-4	0.0	0.0	7.966098369179979	0.0
19	2.452729765592616E-4	0.0	0.0	8.246568017875495	0.0
20	2.452729765592616E-4	0.0	0.0	8.66672062672151	0.0
21	2.452729765592616E-4	0.0	0.0	9.029969905005776	0.0
22	2.452729765592616E-4	0.0	0.0	9.415171114692097	0.0
23	2.452729765592616E-4	0.0	0.0	9.740157808633118	0.0
24	3.6790946483889245E-4	0.0	0.0	10.007627989570993	0.0
25	3.6790946483889245E-4	0.0	0.0	10.332982592976853	0.0
26	3.6790946483889245E-4	0.0	0.0	10.614433333578607	0.0
27	3.6790946483889245E-4	0.0	0.0	11.046236408811186	0.0
28	3.6790946483889245E-4	0.0	0.0	11.382260386697375	0.0
29	3.6790946483889245E-4	0.0	0.0	11.803148814473067	0.0
30	3.6790946483889245E-4	0.0	0.0	13.104199318631672	0.0
31	3.6790946483889245E-4	0.0	0.0	13.525333019383924	0.0
32	3.6790946483889245E-4	0.0	0.0	13.821500138579232	0.0
33	3.6790946483889245E-4	0.0	0.0	14.183645688468982	0.0
34	3.6790946483889245E-4	0.0	0.0	14.593496832299508	0.0
35	3.6790946483889245E-4	0.0	0.0	14.963736390415713	0.0
36	3.6790946483889245E-4	0.0	0.0	15.22065983336154	0.0
37	3.6790946483889245E-4	0.0	0.0	15.58942775361839	0.0
38	3.6790946483889245E-4	0.0	0.0	16.020004463968174	0.0
39	3.6790946483889245E-4	0.0	0.0	17.48992541248783	0.0
40	3.6790946483889245E-4	0.0	0.0	17.86789106936565	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGG	105	0.0	46.000004	2
AACGTAT	20	6.3103717E-4	46.0	15
AATCCCG	20	6.3103717E-4	46.0	14
TCGTTAG	45	3.092282E-10	46.0	1
AAATCCG	20	6.3103717E-4	46.0	38
TTGTGCG	55	1.8189894E-12	46.0	1
ATATGCG	20	6.3103717E-4	46.0	1
TACGGGT	45	3.092282E-10	46.0	4
TATGCGT	30	1.8607334E-6	46.0	11
TCGTAAT	20	6.3103717E-4	46.0	43
TCGTAAG	30	1.8607334E-6	46.0	1
ACGTATC	20	6.3103717E-4	46.0	16
ATACGGA	25	3.4162764E-5	46.0	4
TATCGTC	20	6.3103717E-4	46.0	19
CGTTAGG	125	0.0	44.16	2
CGTTTTT	3060	0.0	42.993465	1
GCAAGGC	1785	0.0	42.134453	46
CGAATAT	110	0.0	41.81818	14
GGGCGAT	490	0.0	41.775513	7
CTAGACG	150	0.0	41.399998	17
>>END_MODULE