##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933070.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1382688
Sequences flagged as poor quality	0
Sequence length	52
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.481464365062834	33.0	31.0	34.0	31.0	34.0
2	32.86845333148187	34.0	31.0	34.0	31.0	34.0
3	32.871231253905435	34.0	31.0	34.0	31.0	34.0
4	36.345384497442666	37.0	37.0	37.0	35.0	37.0
5	36.241533158601214	37.0	37.0	37.0	35.0	37.0
6	36.33216748825476	37.0	37.0	37.0	35.0	37.0
7	36.51812628734754	37.0	37.0	37.0	35.0	37.0
8	36.46667360966465	37.0	37.0	37.0	35.0	37.0
9	38.29156903075748	39.0	39.0	39.0	37.0	39.0
10	37.9996036705316	39.0	39.0	39.0	35.0	39.0
11	37.67665301210396	39.0	37.0	39.0	35.0	39.0
12	37.30813314355805	39.0	35.0	39.0	35.0	39.0
13	37.23174425466917	39.0	37.0	39.0	35.0	39.0
14	38.069720717905994	40.0	37.0	41.0	33.0	41.0
15	38.331019000671155	41.0	37.0	41.0	33.0	41.0
16	38.55072221643639	41.0	37.0	41.0	34.0	41.0
17	38.587409451734594	41.0	37.0	41.0	35.0	41.0
18	38.450987496817795	40.0	37.0	41.0	35.0	41.0
19	38.347909289731305	40.0	36.0	41.0	35.0	41.0
20	38.136354694623805	40.0	35.0	41.0	34.0	41.0
21	38.09318443495568	40.0	35.0	41.0	34.0	41.0
22	38.04002421370548	40.0	35.0	41.0	34.0	41.0
23	38.01528544400472	40.0	35.0	41.0	34.0	41.0
24	37.95751463815409	40.0	35.0	41.0	34.0	41.0
25	37.81417716795112	40.0	35.0	41.0	34.0	41.0
26	37.70423985743711	40.0	35.0	41.0	34.0	41.0
27	37.58061543891319	39.0	35.0	41.0	34.0	41.0
28	37.537871161100696	39.0	35.0	41.0	34.0	41.0
29	37.46909859635724	40.0	35.0	41.0	34.0	41.0
30	37.40426979911592	40.0	35.0	41.0	34.0	41.0
31	37.231697244786965	40.0	35.0	41.0	33.0	41.0
32	37.0869039146937	39.0	35.0	41.0	33.0	41.0
33	36.92767421139114	39.0	35.0	41.0	33.0	41.0
34	36.69655410331181	39.0	35.0	41.0	32.0	41.0
35	36.571806510217776	39.0	35.0	41.0	32.0	41.0
36	36.489740997245946	39.0	35.0	41.0	32.0	41.0
37	36.381793289592444	39.0	35.0	41.0	31.0	41.0
38	36.38246878543822	39.0	35.0	41.0	31.0	41.0
39	36.34060250757944	39.0	35.0	41.0	31.0	41.0
40	36.27398950450138	39.0	35.0	41.0	31.0	41.0
41	36.14114102386077	39.0	35.0	41.0	31.0	41.0
42	36.14276177995325	39.0	35.0	41.0	31.0	41.0
43	36.09998712652457	38.0	35.0	41.0	31.0	41.0
44	36.05377785877942	38.0	35.0	41.0	31.0	41.0
45	35.994151970654265	38.0	35.0	41.0	31.0	41.0
46	35.93846334097063	38.0	35.0	41.0	31.0	41.0
47	35.869375448401954	38.0	35.0	41.0	31.0	41.0
48	35.76636956421116	38.0	35.0	41.0	30.0	41.0
49	35.71927289453586	37.0	35.0	41.0	30.0	41.0
50	35.61311590177972	37.0	35.0	41.0	30.0	41.0
51	35.537850910689905	37.0	35.0	40.0	30.0	41.0
52	35.303450236061934	37.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	4.0
14	12.0
15	44.0
16	85.0
17	182.0
18	319.0
19	695.0
20	1086.0
21	1856.0
22	2859.0
23	4522.0
24	7412.0
25	11833.0
26	15280.0
27	17036.0
28	17251.0
29	17734.0
30	19425.0
31	23211.0
32	28552.0
33	39477.0
34	103288.0
35	150303.0
36	106696.0
37	104813.0
38	150107.0
39	554640.0
40	3959.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.91496273924414	22.194811844754565	25.793888426022356	17.096336989978937
2	39.553536300307805	22.901768150153902	24.611698373024137	12.932997176514153
3	30.722259830127985	23.45554456247541	32.435155291721635	13.387040315674975
4	27.458038255918904	26.718464324562014	31.82901710291837	13.994480316600708
5	24.413316670138165	31.059935430118724	30.44526313962369	14.08148476011942
6	27.2693478210558	36.57122937351015	26.881480131454094	9.277942673979958
7	84.21133328704667	1.7178857413964683	11.687813881367308	2.3829670901895437
8	82.51340866486149	2.2141654516420193	12.044510402925317	3.2279154805711774
9	76.8908097849985	4.064619060843806	14.592879955564813	4.4516911985928855
10	45.405398759517695	24.70376541924136	19.109951051864197	10.78088476937675
11	30.327810757018213	22.87421312689486	31.857440000925735	14.940536115161192
12	31.289849915526858	18.126504316230417	33.143919669513295	17.43972609872943
13	29.344580990071513	19.75253998009674	34.785649401745005	16.11722962808674
14	17.614530537619476	21.581513689277696	39.6105990650096	21.193356708093223
15	15.103262630470503	28.354263579346895	39.46334964937861	17.079124140804
16	27.094398736374366	26.158178851628133	31.78381529311023	14.963607118887268
17	24.892311208313085	26.0630742437918	29.775770094193337	19.268844453701774
18	21.073300701242797	25.574533083385404	35.18320835936958	18.168957856002223
19	21.505213034321553	27.594439237195957	30.029044874910323	20.87130285357217
20	16.861070610289524	37.35759621838043	28.834993867018444	16.946339304311604
21	22.057687634520587	29.09289731305978	33.89918766923557	14.950227383184059
22	19.140615959638037	25.459539679233494	36.53391075933254	18.865933601795923
23	18.832014163715893	25.63984065819621	36.57231421694554	18.955830961142357
24	20.2528697725011	28.85972829734546	32.246320211067136	18.6410817190863
25	23.150848202920688	27.481687842810526	31.24334629359624	18.124117660672546
26	18.27028223286815	30.11510912078502	28.482564396306326	23.1320442500405
27	19.946509986345436	26.840834664074613	33.727059177486176	19.48559617209378
28	17.01142991043533	29.749444560161077	29.285927121664468	23.95319840773913
29	20.402288875002895	29.98897799069638	27.92929424425467	21.679438890046054
30	23.52338343863547	25.854205721030343	28.37726226017728	22.24514858015691
31	20.679719502881344	26.89160533685112	32.55246302853572	19.876212131731815
32	23.61899430674165	26.642814575667106	27.104957879145548	22.633233238445694
33	23.01401328426948	27.42028570436715	28.9713225253998	20.59437848596357
34	17.73711784581916	27.8937113795737	33.604833483764956	20.764337290842185
35	18.09793677243167	31.378879400124976	29.22734557615312	21.29583825129024
36	20.374155268578306	31.13826112615427	26.728372561271957	21.759211043995464
37	17.383386562984562	33.421567266078824	28.107497859242287	21.087548311694324
38	20.177870929667428	29.8404990858386	29.839920502673056	20.141709481820914
39	17.354240436020273	31.892661251128235	27.048546020504986	23.704552292346502
40	23.31531046772663	28.306385822398113	29.048780346687032	19.329523363188226
41	18.74732405285936	27.446900529982184	28.07408468143211	25.731690735726353
42	17.399876183202572	29.98066085769168	30.789592446018187	21.829870513087553
43	17.928411904927213	29.522929250850517	31.123434932537204	21.425223911685066
44	21.295259668124697	27.461437432016478	28.142936078131868	23.100366821726954
45	18.221247309588282	27.592124904533776	27.233981925061908	26.952645860816034
46	21.029328380661436	27.242949964127845	28.301033928116826	23.426687727093892
47	17.550669420722535	26.876200560068504	31.860766854127615	23.71236316508135
48	17.414919345506725	30.52937466731468	29.791319516767338	22.264386470411257
49	21.36996921937559	27.399746001990327	29.574206183896873	21.656078594737206
50	19.201005577541714	27.80547744682821	30.057540095813373	22.935976879816707
51	18.001096415098704	28.490375269041174	29.564659561665398	23.943868754194728
52	18.470399685250758	28.694036543312734	29.646890694068368	23.18867307736814
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	990.0
1	2863.0
2	4736.0
3	26108.0
4	47480.0
5	26881.0
6	6282.0
7	7093.5
8	7905.0
9	10412.0
10	12919.0
11	15885.0
12	18851.0
13	19455.5
14	18556.5
15	17053.0
16	15637.5
17	14222.0
18	12538.5
19	10855.0
20	10268.5
21	9682.0
22	9434.5
23	9187.0
24	9913.5
25	10640.0
26	11784.0
27	12928.0
28	14638.0
29	16348.0
30	17716.5
31	19085.0
32	20985.5
33	22886.0
34	26621.5
35	30357.0
36	31495.0
37	32633.0
38	36324.0
39	42575.5
40	45136.0
41	51625.0
42	58114.0
43	68706.5
44	79299.0
45	90399.0
46	101499.0
47	103311.0
48	105123.0
49	110809.5
50	116496.0
51	100994.5
52	85493.0
53	80583.0
54	75673.0
55	73352.5
56	71032.0
57	67581.5
58	64131.0
59	69245.0
60	74359.0
61	59297.5
62	44236.0
63	38701.5
64	28029.0
65	22891.0
66	19364.0
67	15837.0
68	12044.5
69	8252.0
70	7392.0
71	6532.0
72	5059.0
73	3586.0
74	3134.5
75	2683.0
76	2077.5
77	1472.0
78	1271.5
79	1071.0
80	918.0
81	765.0
82	631.5
83	498.0
84	287.5
85	77.0
86	61.5
87	46.0
88	64.5
89	48.5
90	14.0
91	11.0
92	8.0
93	4.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1382688.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.622169667969697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.91715504929948	16.752170689442664
2	10.77889246791236	5.092416534196528
3	4.624970072322354	3.277554832730422
4	2.588918506035104	2.446234888244314
5	1.6042367187134339	1.8947775978517858
6	1.0481523657550704	1.4855779813050065
7	0.7814852898332071	1.2922264676623745
8	0.5876746532455626	1.110572029482555
9	0.4764179167927478	1.0128622377005063
>10	5.017578091278389	27.18658376107633
>50	1.2246950079117402	20.23701019452441
>100	0.33937218140168884	10.412424691367345
>500	0.003996230396650384	0.7008809634792044
>1k	0.005533242087669763	2.2599998162415327
>5k	3.0740233820387573E-4	0.6373427493221069
>10k+	6.148046764077515E-4	4.201364565372959
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40286	2.913600175889282	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	17572	1.270857923117869	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	8777	0.6347780554976973	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTC	2873	0.20778367932606634	No Hit
GGGGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT	2873	0.20778367932606634	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTT	2528	0.18283228031197205	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCT	2147	0.15527725705292877	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	1995	0.1442841769075887	No Hit
CGCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	1862	0.13466523178041612	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1833	0.13256786780531832	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCG	1750	0.1265650674627971	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTGTCTCTTATACACATCTGA	1657	0.11983903816334561	No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT	1584	0.11455946677775464	No Hit
GGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAG	1526	0.11036473882755907	No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCTT	1431	0.10349406373672153	Illumina Single End Adapter 2 (95% over 21bp)
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1410	0.10197528292716795	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10674859404290772	0.0
2	7.232289569302691E-5	0.0	0.0	0.33637378786826816	0.0
3	7.232289569302691E-5	0.0	0.0	0.6226278090212687	0.0
4	7.232289569302691E-5	0.0	0.0	0.9481531625355829	0.0
5	7.232289569302691E-5	0.0	0.0	2.0086237820824366	0.0
6	7.232289569302691E-5	0.0	0.0	3.3633039413085237	0.0
7	7.232289569302691E-5	0.0	0.0	4.520976532666806	0.0
8	7.232289569302691E-5	0.0	0.0	6.245805272049805	0.0
9	7.232289569302691E-5	0.0	0.0	7.472546228794927	0.0
10	7.232289569302691E-5	0.0	0.0	8.53229361938485	0.0
11	7.232289569302691E-5	0.0	0.0	9.763373949871555	0.0
12	7.232289569302691E-5	0.0	0.0	10.461289893309264	0.0
13	7.232289569302691E-5	0.0	0.0	10.806414751556389	0.0
14	7.232289569302691E-5	0.0	0.0	11.240062834131779	0.0
15	7.232289569302691E-5	0.0	0.0	11.498689509130042	0.0
16	1.4464579138605382E-4	0.0	0.0	11.801650119188132	0.0
17	1.4464579138605382E-4	0.0	0.0	12.155092110439954	0.0
18	1.4464579138605382E-4	0.0	0.0	12.548890277488486	0.0
19	1.4464579138605382E-4	0.0	0.0	12.808818764609224	0.0
20	1.4464579138605382E-4	0.0	0.0	13.167757295933718	0.0
21	1.4464579138605382E-4	0.0	0.0	13.496898794232683	0.0
22	1.4464579138605382E-4	0.0	0.0	13.851714920502673	0.0
23	1.4464579138605382E-4	0.0	0.0	14.177167951121294	0.0
24	2.8929158277210765E-4	0.0	0.0	14.435433011641093	0.0
25	2.8929158277210765E-4	0.0	0.0	14.713586868476474	0.0
26	2.8929158277210765E-4	0.0	0.0	14.953698882177324	0.0
27	2.8929158277210765E-4	0.0	0.0	15.281538568353815	0.0
28	2.8929158277210765E-4	0.0	0.0	15.524254206299613	0.0
29	2.8929158277210765E-4	0.0	0.0	15.86084496285496	0.0
30	2.8929158277210765E-4	0.0	0.0	16.79482283783471	0.0
31	2.8929158277210765E-4	0.0	0.0	17.18095477793978	0.0
32	2.8929158277210765E-4	0.0	0.0	17.553996273924415	0.0
33	2.8929158277210765E-4	0.0	0.0	17.87221701497373	0.0
34	2.8929158277210765E-4	0.0	0.0	18.200852253002846	0.0
35	2.8929158277210765E-4	0.0	0.0	18.531440209215674	0.0
36	2.8929158277210765E-4	0.0	0.0	18.78970526973547	0.0
37	2.8929158277210765E-4	0.0	0.0	19.10170624175519	0.0
38	2.8929158277210765E-4	0.0	0.0	19.462886782846166	0.0
39	2.8929158277210765E-4	0.0	0.0	20.819808951838738	0.0
40	2.8929158277210765E-4	0.0	0.0	21.26076164687912	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGC	25	3.417518E-5	46.0	15
GCGACTT	30	1.8616793E-6	46.0	22
CGGTTCA	20	6.3119014E-4	46.0	39
CCTCGTA	20	6.3119014E-4	46.0	45
ACGGGTA	25	3.417518E-5	46.0	5
TCGATAG	25	3.417518E-5	46.0	1
CGTTAAT	20	6.3119014E-4	46.0	25
AATTCGG	20	6.3119014E-4	46.0	40
GCGATCG	20	6.3119014E-4	46.0	9
CACGTAG	25	3.417518E-5	46.0	1
TCGACGC	20	6.3119014E-4	46.0	15
TATCGCG	25	3.417518E-5	46.0	1
AATCGAG	35	1.01978E-7	45.999996	1
CGTTTTT	9000	0.0	44.44111	1
TGCGTAG	110	0.0	43.909092	1
CGTAAGG	155	0.0	43.032257	2
CGGGATC	295	0.0	42.101696	6
TACCGGG	115	0.0	42.0	3
TAGTACG	55	4.7293724E-11	41.818184	1
GCGAGAC	305	0.0	41.47541	21
>>END_MODULE