##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933069.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1572939
Sequences flagged as poor quality	0
Sequence length	52
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52946872065605	33.0	31.0	34.0	31.0	34.0
2	32.92267150855818	34.0	31.0	34.0	31.0	34.0
3	32.94431316154028	34.0	31.0	34.0	31.0	34.0
4	36.403638030463995	37.0	37.0	37.0	35.0	37.0
5	36.338432704637626	37.0	37.0	37.0	35.0	37.0
6	36.4137293308895	37.0	37.0	37.0	35.0	37.0
7	36.55389306260446	37.0	37.0	37.0	35.0	37.0
8	36.50319370299802	37.0	37.0	37.0	35.0	37.0
9	38.338851029823786	39.0	39.0	39.0	37.0	39.0
10	38.040009180267006	39.0	39.0	39.0	35.0	39.0
11	37.78078615890381	39.0	38.0	39.0	35.0	39.0
12	37.45866305050609	39.0	37.0	39.0	35.0	39.0
13	37.386060743614344	39.0	37.0	39.0	35.0	39.0
14	38.49180673885001	41.0	38.0	41.0	34.0	41.0
15	38.65560902234607	41.0	38.0	41.0	34.0	41.0
16	38.80096367373433	41.0	38.0	41.0	35.0	41.0
17	38.80863402840161	41.0	38.0	41.0	35.0	41.0
18	38.66773981699227	40.0	38.0	41.0	35.0	41.0
19	38.56260477996922	40.0	37.0	41.0	35.0	41.0
20	38.39103042139587	40.0	35.0	41.0	35.0	41.0
21	38.35704690391681	40.0	35.0	41.0	35.0	41.0
22	38.28705944731487	40.0	35.0	41.0	35.0	41.0
23	38.237320074077886	40.0	35.0	41.0	35.0	41.0
24	38.198424096547924	40.0	35.0	41.0	34.0	41.0
25	38.083593832945844	40.0	35.0	41.0	34.0	41.0
26	37.98525181205374	40.0	35.0	41.0	34.0	41.0
27	37.88168072633459	40.0	35.0	41.0	34.0	41.0
28	37.83333810147755	40.0	35.0	41.0	34.0	41.0
29	37.776755487657184	40.0	35.0	41.0	34.0	41.0
30	37.72107437097052	40.0	35.0	41.0	34.0	41.0
31	37.578070096806044	40.0	35.0	41.0	34.0	41.0
32	37.41779306126938	40.0	35.0	41.0	33.0	41.0
33	37.23375095919168	40.0	35.0	41.0	33.0	41.0
34	37.01640686638198	40.0	35.0	41.0	33.0	41.0
35	36.89074655787669	40.0	35.0	41.0	32.0	41.0
36	36.811421803388434	40.0	35.0	41.0	32.0	41.0
37	36.6846482921461	40.0	35.0	41.0	32.0	41.0
38	36.64724569738559	40.0	35.0	41.0	32.0	41.0
39	36.576167289386305	40.0	35.0	41.0	31.0	41.0
40	36.513258937568466	39.0	35.0	41.0	31.0	41.0
41	36.39592253736477	39.0	35.0	41.0	31.0	41.0
42	36.37776862294088	39.0	35.0	41.0	31.0	41.0
43	36.32892121054917	39.0	35.0	41.0	31.0	41.0
44	36.27123620178532	39.0	35.0	41.0	31.0	41.0
45	36.198426003805615	39.0	35.0	41.0	31.0	41.0
46	36.12630114708835	39.0	35.0	41.0	31.0	41.0
47	36.039171894142115	39.0	35.0	41.0	31.0	41.0
48	35.91848126341835	38.0	35.0	41.0	30.0	41.0
49	35.84419166922557	38.0	35.0	41.0	30.0	41.0
50	35.72038775820295	38.0	35.0	41.0	30.0	41.0
51	35.5946606956786	37.0	35.0	40.0	29.0	41.0
52	35.35315864124419	37.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	6.0
13	6.0
14	7.0
15	30.0
16	60.0
17	171.0
18	340.0
19	597.0
20	1083.0
21	1783.0
22	3012.0
23	4781.0
24	7985.0
25	12803.0
26	17367.0
27	19269.0
28	19789.0
29	19898.0
30	21197.0
31	24721.0
32	29817.0
33	40152.0
34	100128.0
35	156989.0
36	101583.0
37	108886.0
38	186211.0
39	690465.0
40	3803.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.54333639130316	21.878470811646224	26.109276964968124	16.46891583208249
2	38.560935929492494	22.850091453006126	25.427559492135423	13.161413125365955
3	29.647303550868788	23.34572415077762	33.07788795369687	13.929084344656722
4	26.630848367292053	26.9149026122437	32.31631995900667	14.137929061457562
5	25.174974999030475	31.534344307058316	29.267377819483148	14.023302874428062
6	27.494136771991794	38.483246966347714	25.054627038937937	8.96798922272256
7	85.75176786893833	1.6505408029173414	10.742756076364055	1.854935251780266
8	84.26881144151172	2.0649243231937158	11.221032729177672	2.4452315061168934
9	79.0609171747919	4.054130516186579	13.090653865153069	3.7942984438684526
10	42.68735151204211	23.189392595644204	19.766500798823095	14.356755093490591
11	29.748070332034494	24.52860536867609	30.32272707333215	15.400597225957268
12	30.06766314523322	19.92048006947504	31.59855531587684	18.413301469414897
13	28.229066734310738	20.472567594801834	34.153835590572804	17.14453008031462
14	19.86644110165747	21.823986817034864	37.06094133338928	21.248630747918387
15	16.28213172920247	27.723325570794543	37.94075930471556	18.05378339528742
16	25.83927285164905	26.492381459166563	32.369723174261686	15.298622514922702
17	24.96829184094234	25.311788950493312	30.886258144785018	18.833661063779335
18	21.33680962834541	25.330607226345077	34.873443916134065	18.45913922917545
19	21.345710164221245	26.976189159274455	31.49816998624867	20.179930690255627
20	19.236791763698402	32.11821946051309	30.99281027427001	17.652178501518495
21	23.21984514339081	27.017449500584572	33.75401080397904	16.008694552045565
22	21.704401760017394	23.91332403863087	35.20568820532773	19.176585996024002
23	19.828423098416405	24.780744834987246	35.51568115483181	19.87515091176454
24	20.453940044718834	26.04150574179927	33.621202093660344	19.883352119821556
25	22.62408141701617	26.181816332356185	32.20639834094011	18.987703909687532
26	20.710720504736674	28.598820424695425	29.34557538467798	21.34488368588992
27	21.717752563831148	26.07723503581512	32.55212058446005	19.652891815893685
28	18.949558755933957	27.80889786571507	30.774302118518264	22.467241259832708
29	21.171768263104926	27.24574824579974	30.771695533011766	20.810787958083562
30	24.5457706878652	25.51688272717505	29.819846796347477	20.11749978861227
31	22.915637542206024	26.075899955433744	31.65418366510081	19.35427883725942
32	24.887869141778545	25.912066520062126	29.102972206805223	20.097092131354106
33	24.19718755781375	26.472164527677172	30.041915166449556	19.288732748059527
34	21.012257945158712	27.047520596793646	32.18980519905731	19.750416258990334
35	21.788066797250245	28.44337892314959	29.877509553771635	19.89104472582853
36	23.54420610080874	27.962877136367016	28.583625938450254	19.90929082437399
37	20.534744195420167	29.609221972371465	29.634715650130104	20.221318182078264
38	22.830573849335543	27.249689911687614	30.25298501721936	19.666751221757487
39	20.934441831501413	28.323348839338337	29.335911945727076	21.406297383433177
40	23.965074297223225	26.449595311706304	29.730014959257794	19.855315431812677
41	20.756049662447175	26.281375183653022	29.62365355554157	23.338921598358233
42	19.504062140998474	28.278782584702906	31.57967346476882	20.637481809529802
43	20.213879877096314	27.27550146572753	31.325118138719937	21.185500518456216
44	22.513015444337004	26.64966664314382	29.794353118588834	21.042964793930345
45	20.740473724664465	26.186965928112915	29.27774058625287	23.79481976096975
46	21.92189271166905	26.621757105647454	29.241820566468245	22.214529616215252
47	20.14165838598954	26.266053546895336	31.494482621385828	22.0978054457293
48	20.234796136404526	27.832484285786034	30.710345410724766	21.222374167084674
49	22.01827280015309	26.830538247192038	30.36099937759824	20.79018957505663
50	20.679568629171253	26.645724977255952	30.738509249246153	21.936197144326638
51	20.425585480428676	26.55029851761575	30.116933968831596	22.90718203312398
52	20.559284244334968	26.842744696393183	30.508493972112078	22.08947708715977
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1163.0
1	3723.5
2	6284.0
3	27402.0
4	48520.0
5	28195.0
6	7870.0
7	8084.5
8	8299.0
9	9745.5
10	11192.0
11	12938.0
12	14684.0
13	15146.5
14	15245.5
15	14882.0
16	13799.5
17	12717.0
18	12092.5
19	11468.0
20	10807.5
21	10147.0
22	10243.5
23	10340.0
24	10967.0
25	11594.0
26	12818.5
27	14043.0
28	16091.0
29	18139.0
30	20601.5
31	23064.0
32	26351.0
33	29638.0
34	31929.0
35	34220.0
36	37220.0
37	40220.0
38	44356.5
39	53412.5
40	58332.0
41	63814.5
42	69297.0
43	74223.5
44	79150.0
45	88712.0
46	98274.0
47	107508.5
48	116743.0
49	123388.0
50	130033.0
51	126047.5
52	122062.0
53	112002.0
54	101942.0
55	97407.5
56	92873.0
57	86416.0
58	79959.0
59	78026.0
60	76093.0
61	63744.0
62	51395.0
63	44181.5
64	30558.5
65	24149.0
66	20145.5
67	16142.0
68	13377.5
69	10613.0
70	8379.5
71	6146.0
72	5037.0
73	3928.0
74	3172.0
75	2416.0
76	2142.0
77	1868.0
78	1429.0
79	990.0
80	705.0
81	420.0
82	316.5
83	213.0
84	157.0
85	101.0
86	110.5
87	120.0
88	107.5
89	60.0
90	25.0
91	15.0
92	5.0
93	2.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1572939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.47497420750753
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.78629861807364	17.0865648271922
2	9.303760907215665	4.368110946594092
3	3.7465695813010513	2.638518728630234
4	2.0250003623686226	1.9014732510718728
5	1.2810087449192382	1.5035823623285356
6	0.9056855798031499	1.2756567375594268
7	0.6936615024195295	1.1398580114627563
8	0.5841152885823597	1.096967306694537
9	0.47294054694097465	0.9992040427011493
>10	6.699481099505429	37.94512850464742
>50	1.4106803029466748	21.865777210321443
>100	0.0823701562742862	2.6635694131745993
>500	0.0035340330787648165	0.5605633614706818
>1k	0.004077730475497865	1.493814567999285
>5k	2.718486983665244E-4	0.34148161967550295
>10k+	5.436973967330488E-4	3.119729108476292
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38698	2.4602352665933007	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	10188	0.6477047107357627	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	5351	0.3401911962256642	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTC	2760	0.17546770726646108	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTT	2419	0.15378854488317728	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT	1995	0.1268326362306485	No Hit
GGGGCTGTCTCTTATACACATCTGACGCGGATACGTTCGTATGCCGTCTTCT	1899	0.12072941163007592	No Hit
GCCCTGCTAGAGGACTTTCGTCTGTATCTATGCTCTGCCCCATGCAGCTCCT	1807	0.11488048805452723	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1722	0.10947659127277028	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.271505125119283E-4	0.0	0.0	0.0842372145391525	0.0
2	1.271505125119283E-4	0.0	0.0	0.22626433701497642	0.0
3	1.271505125119283E-4	0.0	0.0	0.41718083155163677	0.0
4	1.271505125119283E-4	0.0	0.0	0.6288864348839974	0.0
5	1.271505125119283E-4	0.0	0.0	1.3114303860480285	0.0
6	1.271505125119283E-4	0.0	0.0	2.2158519815453745	0.0
7	1.271505125119283E-4	0.0	0.0	2.9292935072498043	0.0
8	1.271505125119283E-4	0.0	0.0	3.992017490824501	0.0
9	1.271505125119283E-4	0.0	0.0	4.69744853424068	0.0
10	1.271505125119283E-4	0.0	0.0	5.407011969313495	0.0
11	1.271505125119283E-4	0.0	0.0	6.22592484514657	0.0
12	1.271505125119283E-4	0.0	0.0	6.687226904539846	0.0
13	1.271505125119283E-4	0.0	0.0	6.938349166750904	0.0
14	1.271505125119283E-4	0.0	0.0	7.23848795153531	0.0
15	1.271505125119283E-4	0.0	0.0	7.427878639921828	0.0
16	1.271505125119283E-4	0.0	0.0	7.6677480817755805	0.0
17	1.271505125119283E-4	0.0	0.0	7.92847020768129	0.0
18	1.271505125119283E-4	0.0	0.0	8.212143001095402	0.0
19	1.271505125119283E-4	0.0	0.0	8.419716212771124	0.0
20	1.271505125119283E-4	0.0	0.0	8.69404344351561	0.0
21	1.271505125119283E-4	0.0	0.0	8.97174016284166	0.0
22	1.271505125119283E-4	0.0	0.0	9.262151933418906	0.0
23	1.271505125119283E-4	0.0	0.0	9.547414108239417	0.0
24	1.271505125119283E-4	0.0	0.0	9.766812317578749	0.0
25	1.271505125119283E-4	0.0	0.0	10.000578534831929	0.0
26	1.271505125119283E-4	0.0	0.0	10.21069475675789	0.0
27	1.271505125119283E-4	0.0	0.0	10.463088524094069	0.0
28	1.271505125119283E-4	0.0	0.0	10.68312248599596	0.0
29	1.271505125119283E-4	0.0	0.0	10.960692054809499	0.0
30	1.271505125119283E-4	0.0	0.0	11.567835752053957	0.0
31	1.271505125119283E-4	0.0	0.0	11.879799534501974	0.0
32	1.271505125119283E-4	0.0	0.0	12.184070710943018	0.0
33	1.271505125119283E-4	0.0	0.0	12.45483772733717	0.0
34	1.9072576876789246E-4	0.0	0.0	12.745821675220718	0.0
35	1.9072576876789246E-4	0.0	0.0	13.022437615190418	0.0
36	2.543010250238566E-4	0.0	0.0	13.272224797020101	0.0
37	2.543010250238566E-4	0.0	0.0	13.547759957633449	0.0
38	2.543010250238566E-4	0.0	0.0	13.862203175075448	0.0
39	2.543010250238566E-4	0.0	0.0	14.730196148738127	0.0
40	3.1787628127982076E-4	0.0	0.0	15.085009653902663	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAT	25	3.417735E-5	46.0	34
CGACGTC	25	3.417735E-5	46.0	42
CGCATTA	20	6.3121686E-4	46.0	29
TAGTTCG	20	6.3121686E-4	46.0	42
TATACGG	110	0.0	43.909092	2
CGTTTTT	9940	0.0	43.80181	1
GGCGATA	135	0.0	40.88889	8
CGATAGG	255	0.0	40.58823	2
CGAACGT	40	2.9114926E-7	40.25	38
CTATACG	40	2.9114926E-7	40.25	1
GCGATCG	40	2.9114926E-7	40.25	9
CGATACG	40	2.9114926E-7	40.25	36
TAGGGAC	760	0.0	39.947365	5
GTATTCG	35	5.374375E-6	39.428574	20
CGTATGG	105	0.0	39.42857	2
AGGGATC	1595	0.0	39.222572	6
CATACGA	575	0.0	38.8	18
ATACGGG	280	0.0	38.607143	3
TACGAAA	585	0.0	38.529915	20
ACGGGAT	430	0.0	38.511627	5
>>END_MODULE