FastQCFastQC Report
Sat 14 Jan 2017
SRR2933067.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933067.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505693
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145700.9676607382779889No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC94060.6246957381086317No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC63920.42452213034131125No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC56810.37730134894696327No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC34000.22580964379856983No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTT24910.165438771383011TruSeq Adapter, Index 14 (95% over 23bp)
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC23740.15766826305229553No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22200.14744041448024264No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT18620.1236639872802756TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT16120.10706033700096898TruSeq Adapter, Index 15 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAC351.0198528E-746.00000434
TGACGTT206.312081E-446.014
CCGCTTA206.312081E-446.010
CCGTATG253.4176646E-546.030
CTAACGG1250.046.01
ACGATAA206.312081E-446.041
CCCGTAG253.4176646E-546.025
GGTATCG301.8617902E-646.07
GCGATCG206.312081E-446.08
ACGTCGT453.110472E-1046.017
CACCGTA253.4176646E-546.028
CGATATC405.6134013E-946.09
CCGCAAT206.312081E-446.032
TACGTTC253.4176646E-546.018
GTACGTT206.312081E-446.037
ATCGACG206.312081E-446.014
TACCGTT301.8617902E-646.023
CTTACGA253.4176646E-546.025
TCACGAC2600.045.99999624
TACGGGA6500.043.5230753
Produced by FastQC (version 0.11.5)