##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933067.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1505693
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51996588946086	33.0	31.0	34.0	31.0	34.0
2	32.88016547862014	34.0	31.0	34.0	31.0	34.0
3	32.96118000150097	34.0	31.0	34.0	31.0	34.0
4	36.387084883837545	37.0	37.0	37.0	35.0	37.0
5	36.366192178618086	37.0	37.0	37.0	35.0	37.0
6	36.497519082575266	37.0	37.0	37.0	35.0	37.0
7	36.53809109825177	37.0	37.0	37.0	35.0	37.0
8	36.39705371546524	37.0	37.0	37.0	35.0	37.0
9	38.04819774017678	39.0	39.0	39.0	35.0	39.0
10	37.94141036718641	39.0	38.0	39.0	35.0	39.0
11	37.495099598656566	39.0	37.0	39.0	35.0	39.0
12	37.33988668340757	39.0	37.0	39.0	35.0	39.0
13	37.21846153233096	39.0	37.0	39.0	34.0	39.0
14	38.65220333759937	41.0	38.0	41.0	35.0	41.0
15	38.75272980614242	41.0	38.0	41.0	35.0	41.0
16	38.75125340955959	41.0	38.0	41.0	35.0	41.0
17	38.74489553979463	41.0	37.0	41.0	35.0	41.0
18	38.678066511566435	40.0	37.0	41.0	35.0	41.0
19	38.548285739523266	40.0	37.0	41.0	35.0	41.0
20	38.461269993285484	40.0	35.0	41.0	35.0	41.0
21	38.420822837059085	40.0	35.0	41.0	35.0	41.0
22	38.376034822503655	40.0	35.0	41.0	35.0	41.0
23	38.33395785196584	40.0	35.0	41.0	35.0	41.0
24	38.28110710483478	40.0	35.0	41.0	35.0	41.0
25	38.21112471134554	40.0	35.0	41.0	35.0	41.0
26	38.11948584472399	40.0	35.0	41.0	34.0	41.0
27	38.06466856125385	40.0	35.0	41.0	34.0	41.0
28	38.05925377882477	40.0	35.0	41.0	34.0	41.0
29	38.02205960976109	40.0	35.0	41.0	34.0	41.0
30	37.93689749504049	40.0	35.0	41.0	34.0	41.0
31	37.87168366991146	40.0	35.0	41.0	34.0	41.0
32	37.77602339919227	40.0	35.0	41.0	34.0	41.0
33	37.61436295446681	40.0	35.0	41.0	34.0	41.0
34	37.496458441395426	40.0	35.0	41.0	34.0	41.0
35	37.35434049304872	40.0	35.0	41.0	33.0	41.0
36	37.27795905274183	40.0	35.0	41.0	33.0	41.0
37	37.16706659325639	40.0	35.0	41.0	33.0	41.0
38	37.153321427409175	40.0	35.0	41.0	33.0	41.0
39	37.052053107771634	40.0	35.0	41.0	33.0	41.0
40	37.00580729272169	39.0	35.0	41.0	33.0	41.0
41	36.95971091052426	39.0	35.0	41.0	33.0	41.0
42	36.89081240332525	39.0	35.0	41.0	33.0	41.0
43	36.78324731535579	39.0	35.0	41.0	33.0	41.0
44	36.69126574939247	39.0	35.0	41.0	33.0	41.0
45	36.62543426847306	39.0	35.0	41.0	33.0	41.0
46	36.52970160583864	39.0	35.0	41.0	32.0	41.0
47	36.43786150297571	38.0	35.0	41.0	32.0	41.0
48	36.38250626123652	38.0	35.0	41.0	32.0	41.0
49	36.286513253365726	38.0	35.0	41.0	32.0	41.0
50	36.18153966313186	38.0	35.0	41.0	32.0	41.0
51	36.07261440413152	37.0	35.0	41.0	31.0	41.0
52	35.81786127716606	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	3.0
12	5.0
13	8.0
14	17.0
15	22.0
16	52.0
17	133.0
18	226.0
19	445.0
20	817.0
21	1329.0
22	2115.0
23	3456.0
24	5388.0
25	8334.0
26	11379.0
27	13073.0
28	14023.0
29	15121.0
30	17599.0
31	21462.0
32	27002.0
33	36991.0
34	91782.0
35	180917.0
36	95346.0
37	118265.0
38	186871.0
39	645431.0
40	8077.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.46705271260476	22.09109028201632	23.488918391730586	19.95293861364833
2	27.885631400292095	23.781209051247497	26.76508425024225	21.56807529821816
3	28.1166213829778	24.10013196581242	26.581912780360938	21.20133387084884
4	23.831684148096592	28.412033528747227	24.270219759273637	23.486062563882545
5	22.49442615460124	37.53514162581615	22.237268819075336	17.733163400507273
6	87.28572159132041	3.052614311151078	7.4447447122354955	2.216919385293018
7	88.70772461584134	2.385878130535242	5.98036917220177	2.926028081421644
8	81.8720017958508	4.38535611177046	9.481149211691891	4.261492880686833
9	41.259539627267976	28.47957717808345	14.918645434361451	15.342237760287123
10	34.69286235640333	21.816399491795472	25.13905557108919	18.351682580712
11	32.009911715070736	19.36497014995753	30.037929378698045	18.58718875627369
12	23.647250800794055	27.321306534599017	30.499444441861655	18.531998222745276
13	17.997759171358304	31.78091417041854	31.140743830249594	19.080582827973565
14	17.007185395694872	25.96830828063888	38.20187780643199	18.822628517234257
15	21.229892149329245	20.406616753880108	37.58269448021609	20.78079661657456
16	20.465592919672204	22.966567553943598	28.981007416518505	27.58683210986569
17	22.003290179339345	26.31060913479707	30.711506263228962	20.974594422634627
18	25.462428263928967	29.937178428803215	25.852547630891554	18.747845676376258
19	24.63868796627201	25.965518867391957	26.149088824879975	23.24670434145606
20	22.889061714439794	28.04735095401254	28.202030560014556	20.861556771533106
21	20.226101868043486	25.68412020245827	27.914189678772498	26.175588250725745
22	18.10654628798832	30.094514618849928	27.32615480048058	24.472784292681176
23	19.16732029703266	28.73241756453673	32.07599424318238	20.024267895248236
24	23.730003393786117	25.177044722928247	28.73547263618812	22.357479247097515
25	17.94668634309916	28.990039802270452	26.70424847561887	26.359025379011523
26	17.05062054482554	26.954963594836396	31.08887402677704	24.905541833561024
27	19.17289912352651	25.671634257448233	32.748574908696526	22.406891710328733
28	18.65127884635181	29.13900775257639	28.2700391115586	23.939674289513203
29	21.23507248821639	26.84099613931924	29.467892857308893	22.45603851515548
30	25.20354414877402	26.776175488628823	26.636571997080416	21.383708365516743
31	24.7620198805467	29.237766264437703	24.646259230799373	21.353954624216225
32	24.262582080145158	27.99415285851764	23.519867595851213	24.223397465485995
33	18.37811559195666	27.393432791412327	29.137613045952925	25.090838570678088
34	20.311909532686943	28.26957420935078	27.802148246687736	23.616368011274545
35	24.592197745489948	30.880797081476768	24.79961054477905	19.72739462825423
36	19.85484424779819	31.838429214986057	25.577923255271823	22.72880328194393
37	22.27333194748199	30.999612802875486	26.279992003682022	20.447063245960496
38	20.303939780552874	29.363090616745914	24.619228488144664	25.713741114556555
39	23.02381694010665	26.155132553581637	27.91744399422724	22.90360651208447
40	18.81246708326332	23.97826117276231	30.569379016838095	26.639892727136278
41	19.817120754363604	29.046292969416744	25.501413634784782	25.635172641434874
42	19.534061724401987	25.89146658714625	27.539080011662403	27.03539167678936
43	20.675728717607107	26.560859351806776	30.085282989294633	22.678128941291483
44	20.89868253355764	29.22056488274834	25.33331827935708	24.54743430433694
45	22.127618312630794	27.472532581342946	27.35810022361796	23.0417488824083
46	23.050183536750186	27.199568570751143	26.838339555274548	22.911908337224123
47	19.996041689773413	28.614066745345827	28.58623902747771	22.803652537403043
48	20.999499898053585	25.884692297832295	28.118613821011323	24.997193983102797
49	19.557041176388545	26.074770886229796	30.456075707332104	23.91211223004955
50	18.981226584702192	27.176589118764582	30.1017538103717	23.74043048616152
51	19.354343813778772	24.426360486500236	28.944213727499562	27.275081972221425
52	19.42999004445129	24.233293241052458	30.13921164540182	26.19750506909443
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2552.0
1	2703.0
2	2854.0
3	11054.0
4	19254.0
5	11779.0
6	4304.0
7	4715.5
8	5127.0
9	6267.0
10	7407.0
11	8558.0
12	9709.0
13	9583.5
14	8710.5
15	7963.0
16	7221.0
17	6479.0
18	6125.0
19	5771.0
20	5699.5
21	5628.0
22	5368.0
23	5108.0
24	5479.5
25	5851.0
26	6865.5
27	7880.0
28	9449.0
29	11018.0
30	12132.5
31	13247.0
32	16883.0
33	20519.0
34	23698.0
35	26877.0
36	33514.5
37	40152.0
38	41836.5
39	46846.0
40	50171.0
41	60036.5
42	69902.0
43	89053.0
44	108204.0
45	120202.5
46	132201.0
47	135624.5
48	139048.0
49	140860.0
50	142672.0
51	128934.0
52	115196.0
53	101809.0
54	88422.0
55	84739.0
56	81056.0
57	77501.5
58	73947.0
59	70085.5
60	66224.0
61	60840.5
62	55457.0
63	47651.5
64	32637.0
65	25428.0
66	22500.0
67	19572.0
68	16411.0
69	13250.0
70	10617.0
71	7984.0
72	7498.5
73	7013.0
74	5269.5
75	3526.0
76	3164.5
77	2803.0
78	2215.0
79	1627.0
80	1283.5
81	940.0
82	648.0
83	356.0
84	234.0
85	112.0
86	68.5
87	25.0
88	21.5
89	12.5
90	7.0
91	6.0
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1505693.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.393730582308237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.4410535946965	16.446191678804933
2	10.581853418563124	4.7393434903356
3	3.9354929760043524	2.6439110823962384
4	1.9611203231077017	1.756672006206526
5	1.2392131861926488	1.3875303112820976
6	0.8131517433939652	1.092570063845922
7	0.627340520481478	0.9833946219319071
8	0.4858932420026994	0.8704769882538198
9	0.4140595785885078	0.8345104783141615
>10	4.452145055575632	23.459915664376837
>50	1.4346393890960047	23.139758338709708
>100	0.6045262350697657	18.170264715487843
>500	0.005647000228874573	0.9194118670581595
>1k	0.0026748948452563768	1.156752443135284
>5k	8.91631615085459E-4	1.4295676482222535
>10k+	2.9721053836181965E-4	0.9697286016387276
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14570	0.9676607382779889	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	9406	0.6246957381086317	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	6392	0.42452213034131125	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5681	0.37730134894696327	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3400	0.22580964379856983	No Hit
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCTT	2491	0.165438771383011	TruSeq Adapter, Index 14 (95% over 23bp)
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2374	0.15766826305229553	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2220	0.14744041448024264	No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	1862	0.1236639872802756	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	1612	0.10706033700096898	TruSeq Adapter, Index 15 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17281079210702313	0.0
2	0.0	0.0	0.0	0.4814394434987743	0.0
3	0.0	0.0	0.0	0.8739497361015824	0.0
4	0.0	0.0	0.0	1.186828921964836	0.0
5	0.0	0.0	0.0	2.73289442137275	0.0
6	0.0	0.0	0.0	3.2648753763217337	0.0
7	0.0	0.0	0.0	4.567464948033895	0.0
8	0.0	0.0	0.0	6.164071958892018	0.0
9	0.0	0.0	0.0	7.580695400722458	0.0
10	0.0	0.0	0.0	8.463810351778218	0.0
11	0.0	0.0	0.0	9.259523687763707	0.0
12	0.0	0.0	0.0	9.811362608446743	0.0
13	6.641460111722642E-5	0.0	0.0	10.051252147682163	0.0
14	6.641460111722642E-5	0.0	0.0	10.203806486448432	0.0
15	6.641460111722642E-5	0.0	0.0	10.617835109813223	0.0
16	6.641460111722642E-5	0.0	0.0	11.163497472592354	0.0
17	1.3282920223445284E-4	0.0	0.0	11.819142414821613	0.0
18	1.9924380335167925E-4	0.0	0.0	12.14656639832954	6.641460111722642E-5
19	1.9924380335167925E-4	0.0	0.0	12.56105992390215	6.641460111722642E-5
20	1.9924380335167925E-4	0.0	0.0	13.003248338140644	6.641460111722642E-5
21	1.9924380335167925E-4	0.0	0.0	13.44942162844617	6.641460111722642E-5
22	1.9924380335167925E-4	0.0	0.0	13.915519299086865	6.641460111722642E-5
23	1.9924380335167925E-4	0.0	0.0	14.262469175323256	6.641460111722642E-5
24	1.9924380335167925E-4	0.0	0.0	14.544864059273703	6.641460111722642E-5
25	1.9924380335167925E-4	0.0	0.0	14.813444706191767	6.641460111722642E-5
26	1.9924380335167925E-4	0.0	0.0	15.111513436005879	6.641460111722642E-5
27	1.9924380335167925E-4	0.0	0.0	15.456005972000932	6.641460111722642E-5
28	1.9924380335167925E-4	0.0	0.0	15.79106763463734	6.641460111722642E-5
29	1.9924380335167925E-4	0.0	0.0	16.10454455191065	6.641460111722642E-5
30	1.9924380335167925E-4	0.0	0.0	16.40885625422978	6.641460111722642E-5
31	1.9924380335167925E-4	0.0	0.0	16.696099404061783	6.641460111722642E-5
32	2.656584044689057E-4	0.0	0.0	17.02471885038982	6.641460111722642E-5
33	2.656584044689057E-4	0.0	0.0	17.360511073638516	6.641460111722642E-5
34	3.320730055861321E-4	0.0	0.0	17.76776540768935	6.641460111722642E-5
35	3.320730055861321E-4	0.0	0.0	18.085891347040864	6.641460111722642E-5
36	3.320730055861321E-4	0.0	0.0	18.41550701238566	6.641460111722642E-5
37	3.320730055861321E-4	0.0	0.0	18.784971438400788	6.641460111722642E-5
38	3.984876067033585E-4	0.0	0.0	19.50649966493834	6.641460111722642E-5
39	3.984876067033585E-4	0.0	0.0	19.890973790805962	6.641460111722642E-5
40	3.984876067033585E-4	0.0	0.0	20.24728812579988	6.641460111722642E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTAC	35	1.0198528E-7	46.000004	34
TGACGTT	20	6.312081E-4	46.0	14
CCGCTTA	20	6.312081E-4	46.0	10
CCGTATG	25	3.4176646E-5	46.0	30
CTAACGG	125	0.0	46.0	1
ACGATAA	20	6.312081E-4	46.0	41
CCCGTAG	25	3.4176646E-5	46.0	25
GGTATCG	30	1.8617902E-6	46.0	7
GCGATCG	20	6.312081E-4	46.0	8
ACGTCGT	45	3.110472E-10	46.0	17
CACCGTA	25	3.4176646E-5	46.0	28
CGATATC	40	5.6134013E-9	46.0	9
CCGCAAT	20	6.312081E-4	46.0	32
TACGTTC	25	3.4176646E-5	46.0	18
GTACGTT	20	6.312081E-4	46.0	37
ATCGACG	20	6.312081E-4	46.0	14
TACCGTT	30	1.8617902E-6	46.0	23
CTTACGA	25	3.4176646E-5	46.0	25
TCACGAC	260	0.0	45.999996	24
TACGGGA	650	0.0	43.523075	3
>>END_MODULE