SRR2933066.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933066.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1974832
Sequences flagged as poor quality	0
Sequence length	52
%GC	42

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58552	2.964910432887456	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	8207	0.4155796543705997	Illumina PCR Primer Index 2 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTT	7155	0.36230930023414654	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	5965	0.302051009908691	Illumina PCR Primer Index 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	5160	0.2612880488061769	Illumina PCR Primer Index 2 (95% over 24bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCT	3237	0.1639126771289912	No Hit
CGTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	2968	0.15029126528231262	Illumina PCR Primer Index 2 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	2750	0.13925235159243926	Illumina PCR Primer Index 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	2590	0.13115039659069735	Illumina PCR Primer Index 2 (96% over 25bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTC	2518	0.12750451683991348	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCG	2483	0.12573221418328243	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2451	0.12411182318293405	No Hit
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCTT	2415	0.1222888833075421	Illumina PCR Primer Index 2 (96% over 26bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	23340	44.472576	1
CGTTCTG	700	42.057144	1
GCGCGAC	330	41.81818	8
ATTGCGG	375	41.706665	1
ATAGCGG	265	41.660378	1
CCCGCAC	1045	41.598083	32
CCGCACT	1035	41.555557	33
CTAGCGG	375	41.09333	1
CGCACTT	1050	40.961903	34
CAACGCA	1170	40.692307	16
TCAACGC	1165	40.669533	15
CATGCGG	215	40.651165	1
TATGCGG	375	40.48	1
ACCCGCA	1085	40.064514	31
CGACGGT	230	40.0	27
CGTTTTC	955	39.979057	1
ATGACGG	190	39.94737	1
TACGGGA	710	39.84507	3
CGTTTCT	855	39.54386	1
ACGCAAG	1205	39.510372	18