##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933066.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1974832
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50307874290066	33.0	31.0	34.0	31.0	34.0
2	32.86644484189035	34.0	31.0	34.0	31.0	34.0
3	32.84683152794769	34.0	31.0	34.0	31.0	34.0
4	36.360844365495396	37.0	37.0	37.0	35.0	37.0
5	36.34121332852617	37.0	37.0	37.0	35.0	37.0
6	36.478979984120166	37.0	37.0	37.0	35.0	37.0
7	36.524079516637364	37.0	37.0	37.0	35.0	37.0
8	36.3688374504768	37.0	37.0	37.0	35.0	37.0
9	38.025227462386674	39.0	39.0	39.0	35.0	39.0
10	37.98786124591864	39.0	39.0	39.0	35.0	39.0
11	37.875576757921685	39.0	38.0	39.0	35.0	39.0
12	37.8523849117292	39.0	38.0	39.0	35.0	39.0
13	37.864150469508296	39.0	38.0	39.0	35.0	39.0
14	39.367747737529065	41.0	39.0	41.0	35.0	41.0
15	39.36270427054048	41.0	39.0	41.0	35.0	41.0
16	39.33214774725141	41.0	39.0	41.0	35.0	41.0
17	39.29432630218672	41.0	39.0	41.0	35.0	41.0
18	39.07455874727572	40.0	39.0	41.0	35.0	41.0
19	38.843939130012075	40.0	37.0	41.0	35.0	41.0
20	38.59371987085484	40.0	36.0	41.0	35.0	41.0
21	38.53839263289232	40.0	36.0	41.0	35.0	41.0
22	38.474836340508965	40.0	36.0	41.0	35.0	41.0
23	38.43006746903028	40.0	36.0	41.0	35.0	41.0
24	38.37595349882927	40.0	35.0	41.0	35.0	41.0
25	38.3116214442545	40.0	35.0	41.0	35.0	41.0
26	38.218771014445785	40.0	35.0	41.0	35.0	41.0
27	38.12697991525355	40.0	35.0	41.0	35.0	41.0
28	38.07659031249241	40.0	35.0	41.0	35.0	41.0
29	37.99182006368137	40.0	35.0	41.0	34.0	41.0
30	37.919533914783635	40.0	35.0	41.0	34.0	41.0
31	37.75770293371791	40.0	35.0	41.0	34.0	41.0
32	37.45649402075721	40.0	35.0	41.0	33.0	41.0
33	37.03369400536349	40.0	35.0	41.0	32.0	41.0
34	36.66268472457404	40.0	35.0	41.0	31.0	41.0
35	36.36385829275604	40.0	35.0	41.0	30.0	41.0
36	36.211374435901384	40.0	35.0	41.0	29.0	41.0
37	36.089952968151216	40.0	35.0	41.0	27.0	41.0
38	36.005678964084034	40.0	35.0	41.0	26.0	41.0
39	35.966787048214734	40.0	35.0	41.0	26.0	41.0
40	35.926942139888354	40.0	35.0	41.0	26.0	41.0
41	35.84850559439993	40.0	35.0	41.0	25.0	41.0
42	35.79440023252611	40.0	35.0	41.0	24.0	41.0
43	35.70039021040777	40.0	35.0	41.0	24.0	41.0
44	35.60221831527948	39.0	35.0	41.0	24.0	41.0
45	35.52525126187949	39.0	35.0	41.0	23.0	41.0
46	35.436986538601765	39.0	35.0	41.0	23.0	41.0
47	35.361540627253355	39.0	35.0	41.0	23.0	41.0
48	35.272889035624296	39.0	35.0	41.0	23.0	41.0
49	35.194336530904906	39.0	35.0	41.0	23.0	41.0
50	35.092797260729014	39.0	35.0	41.0	23.0	41.0
51	35.002620476070874	38.0	35.0	41.0	23.0	41.0
52	34.79026266538116	38.0	35.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	4.0
12	9.0
13	14.0
14	14.0
15	31.0
16	86.0
17	169.0
18	365.0
19	755.0
20	1450.0
21	2462.0
22	3939.0
23	6758.0
24	12753.0
25	25283.0
26	37871.0
27	39923.0
28	35789.0
29	30138.0
30	28702.0
31	30597.0
32	35103.0
33	43193.0
34	78265.0
35	128847.0
36	135542.0
37	144611.0
38	238454.0
39	906244.0
40	7457.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.118865807319306	19.476036442593596	21.175978513615334	29.229119236471764
2	36.85888217326841	20.69482366094939	22.378865645280204	20.067428520502
3	23.45865369813736	22.30144133779481	33.82110478258404	20.418800181483792
4	21.980249459194503	25.43963233328202	32.813170943148585	19.766947264374892
5	20.868863781830555	32.74248138575838	29.909734093836843	16.478920738574217
6	78.06258962787722	2.8466725270807847	16.329389031573317	2.76134881346869
7	78.77925818500005	2.1839326079382952	15.477519100358917	3.559290106702748
8	72.04633102967746	4.868667309421763	18.598442804248666	4.48655885665211
9	31.10269633062458	28.55994839054664	25.377146005331085	14.960209273497696
10	22.773835951615123	23.252357668905507	35.50317191538318	18.470634464096186
11	21.969970103786043	20.031273546306725	38.61062611908254	19.3881302308247
12	19.12213292067376	22.489406693835225	39.15700170951251	19.231458675978512
13	16.804771241300525	24.38683391802442	38.46352499858216	20.344869842092898
14	16.190946875481053	24.75400439125961	41.18198408776038	17.87306464549896
15	19.30078102846217	22.700715807724404	38.38706279825322	19.61144036556021
16	18.082702731169032	25.57589708896757	36.675777990229044	19.66562218963436
17	17.754674828036006	25.680766769021364	38.491021008369316	18.07353739457331
18	18.954017354387613	30.09106597421958	34.15450023090572	16.80041644048709
19	20.309322514522755	25.361347193077687	34.75829842741053	19.57103186498902
20	18.586289871746054	27.722965801647938	33.41448791593412	20.276256410671895
21	17.741610425595695	26.101663331361856	34.08659572054737	22.070130522495077
22	17.039373475819716	28.471738355465178	33.211432668702955	21.277455500012152
23	16.2381407633662	27.177096583405575	36.93412908034709	19.650633572881134
24	18.171925510625712	28.848783086358736	33.52183881970719	19.457452583308353
25	18.694400333800544	27.418889302988813	34.011095627374885	19.875614735835757
26	16.765780582854642	25.906102392507314	33.46517577191376	23.862941252724283
27	18.650295316259815	25.809081481361453	34.251723690926624	21.288899511452115
28	19.32660601003022	24.814009495491263	32.38265330924352	23.476731185234996
29	18.666347314607016	25.47062231116368	34.310665413564294	21.552364960665006
30	19.76092143534235	26.151642266278852	31.43735770941528	22.650078588963517
31	20.002764792144344	24.932348675735454	31.61008126260867	23.45480526951153
32	20.321019712056522	26.180302932097515	30.106864786472975	23.391812569372988
33	17.77032172863312	24.96176889983553	33.71501980927998	23.552889562251373
34	18.37244889691883	28.252833658761862	32.2879110729419	21.086806371377413
35	19.294198190023256	30.44896983642153	31.15064977679114	19.10618219676408
36	19.079141921945766	31.252835684250606	30.06883623518355	19.599186158620075
37	17.89600330559764	30.135424177854116	33.488671441418816	18.47990107512943
38	17.454345483565184	31.64947701880464	29.505294627593635	21.39088287003654
39	18.787319630226772	28.840529219700713	31.777842368363483	20.594308781709024
40	17.3674520161715	28.13581104620545	31.9190695714876	22.577667366135447
41	18.051358292756042	30.073393584872026	29.57284467742066	22.302403444951267
42	17.757054777317766	27.71040777139524	32.321331637324086	22.21120581396291
43	20.06803616712713	27.50112414625649	32.03862404498206	20.392215641634326
44	19.59660366046327	29.096956095505845	30.19715094752364	21.109289296507246
45	19.45051528433811	28.38656655350936	30.61561692336361	21.54730123878892
46	19.94539282328826	27.987798455767376	30.111624685036496	21.955184035907866
47	19.285741774490184	26.104347103956183	31.024765650951576	23.585145470602058
48	19.119449148079433	26.10763852317564	31.05261612127006	23.720296207474863
49	18.83638709520607	26.063837328947475	32.92457282442253	22.17520275142392
50	18.19218039813007	24.994683092030108	34.487693130352355	22.32544337948747
51	17.12763414811994	25.006177740688827	34.703508956711254	23.162679154479974
52	16.29637356494122	26.21777447398057	33.67658616024047	23.809265800837743
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1600.0
1	1999.0
2	2398.0
3	40635.5
4	78873.0
5	48237.0
6	17601.0
7	17941.0
8	18281.0
9	19669.5
10	21058.0
11	22214.0
12	23370.0
13	23293.5
14	22275.5
15	21334.0
16	19853.0
17	18372.0
18	17402.0
19	16432.0
20	15840.5
21	15249.0
22	14961.5
23	14674.0
24	14845.5
25	15017.0
26	16229.0
27	17441.0
28	19449.5
29	21458.0
30	23556.0
31	25654.0
32	31611.5
33	37569.0
34	45550.5
35	53532.0
36	62961.5
37	72391.0
38	77231.5
39	80718.0
40	79364.0
41	82246.0
42	85128.0
43	92797.5
44	100467.0
45	119756.0
46	139045.0
47	154097.0
48	169149.0
49	171894.5
50	174640.0
51	163101.0
52	151562.0
53	131760.5
54	111959.0
55	98277.5
56	84596.0
57	76315.0
58	68034.0
59	62558.0
60	57082.0
61	52247.5
62	47413.0
63	42618.5
64	31524.0
65	25224.0
66	20638.5
67	16053.0
68	13136.0
69	10219.0
70	8452.5
71	6686.0
72	6121.5
73	5557.0
74	4452.0
75	3347.0
76	2590.0
77	1833.0
78	1454.5
79	1076.0
80	828.5
81	581.0
82	394.0
83	207.0
84	162.5
85	118.0
86	77.5
87	37.0
88	27.0
89	12.5
90	8.0
91	8.0
92	8.0
93	5.0
94	2.0
95	1.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1974832.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.86580756733821
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.91475159344822	28.270738407099977
2	10.511877891920479	8.381290024213172
3	4.945284766176364	5.914433125622281
4	3.151125022906375	5.02488575135239
5	2.149015197719785	4.283611316579111
6	1.6152955829382758	3.8637037724273218
7	1.284171774671588	3.583618139672531
8	0.9988806841984977	3.185694811119075
9	0.7972321978491235	2.8604074847345333
>10	3.449701674400489	21.400972775841755
>50	0.1298155825574647	3.5257757184239193
>100	0.04798635195967192	3.0713541683373498
>500	0.002047022843688301	0.5410492304611663
>1k	0.0021749617714188203	1.7551457502478933
>5k	5.117557109220752E-4	1.3509399478907342
>10k+	1.279389277305188E-4	2.9863795759768292
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58552	2.964910432887456	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTC	8207	0.4155796543705997	Illumina PCR Primer Index 2 (95% over 21bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTT	7155	0.36230930023414654	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	5965	0.302051009908691	Illumina PCR Primer Index 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGC	5160	0.2612880488061769	Illumina PCR Primer Index 2 (95% over 24bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCT	3237	0.1639126771289912	No Hit
CGTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTG	2968	0.15029126528231262	Illumina PCR Primer Index 2 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCT	2750	0.13925235159243926	Illumina PCR Primer Index 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCT	2590	0.13115039659069735	Illumina PCR Primer Index 2 (96% over 25bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTC	2518	0.12750451683991348	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCG	2483	0.12573221418328243	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2451	0.12411182318293405	No Hit
CTGTCTCTTATACACATCTGACGCCCTGTAACTCGTATGCCGTCTTCTGCTT	2415	0.1222888833075421	Illumina PCR Primer Index 2 (96% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1273526051836308	0.0
2	0.0	0.0	0.0	0.35233376813825174	0.0
3	0.0	0.0	0.0	0.8023467312662546	0.0
4	0.0	0.0	0.0	1.1837969001920163	0.0
5	0.0	0.0	0.0	2.8417100796422177	0.0
6	0.0	0.0	0.0	4.132908520826075	0.0
7	0.0	0.0	0.0	6.204578414771484	0.0
8	0.0	0.0	0.0	8.162365203723658	0.0
9	0.0	0.0	0.0	10.372831714292658	0.0
10	0.0	0.0	0.0	11.466899462840383	0.0
11	0.0	0.0	0.0	12.318364296304699	0.0
12	0.0	0.0	0.0	12.962824179474508	0.0
13	5.0637218760887E-5	0.0	0.0	13.331007397084917	0.0
14	5.0637218760887E-5	0.0	0.0	13.570065706855065	0.0
15	5.0637218760887E-5	0.0	0.0	13.855457071791424	0.0
16	5.0637218760887E-5	0.0	0.0	14.156343425668615	0.0
17	5.0637218760887E-5	0.0	0.0	14.544680256345856	5.0637218760887E-5
18	5.0637218760887E-5	0.0	0.0	14.761610101517496	5.0637218760887E-5
19	5.0637218760887E-5	0.0	0.0	15.01864462394776	5.0637218760887E-5
20	5.0637218760887E-5	0.0	0.0	15.257702933717907	5.0637218760887E-5
21	5.0637218760887E-5	0.0	0.0	15.530738817276609	5.0637218760887E-5
22	5.0637218760887E-5	0.0	0.0	15.866210391567485	5.0637218760887E-5
23	5.0637218760887E-5	0.0	0.0	16.112712372495483	5.0637218760887E-5
24	5.0637218760887E-5	0.0	0.0	16.32037560663388	5.0637218760887E-5
25	5.0637218760887E-5	0.0	0.0	16.523734677177604	5.0637218760887E-5
26	5.0637218760887E-5	0.0	0.0	16.73879094525509	5.0637218760887E-5
27	5.0637218760887E-5	0.0	0.0	16.976785873431258	5.0637218760887E-5
28	5.0637218760887E-5	0.0	0.0	17.215692271545123	5.0637218760887E-5
29	1.01274437521774E-4	0.0	0.0	17.496678198449285	5.0637218760887E-5
30	1.01274437521774E-4	0.0	0.0	17.731179158530953	5.0637218760887E-5
31	1.01274437521774E-4	0.0	0.0	17.98907451368015	5.0637218760887E-5
32	1.5191165628266102E-4	0.0	0.0	18.25218550236172	5.0637218760887E-5
33	1.5191165628266102E-4	0.0	0.0	18.51600541210594	5.0637218760887E-5
34	1.5191165628266102E-4	0.0	0.0	18.815322012201545	5.0637218760887E-5
35	1.5191165628266102E-4	0.0	0.0	19.074128837288438	5.0637218760887E-5
36	1.5191165628266102E-4	0.0	0.0	19.321744837029176	5.0637218760887E-5
37	1.5191165628266102E-4	0.0	0.0	19.608807230184645	5.0637218760887E-5
38	1.5191165628266102E-4	0.0	0.0	19.908478290811573	5.0637218760887E-5
39	1.5191165628266102E-4	0.0	0.0	20.187641277840342	5.0637218760887E-5
40	1.5191165628266102E-4	0.0	0.0	20.45586662561676	5.0637218760887E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	23340	0.0	44.472576	1
CGTTCTG	700	0.0	42.057144	1
GCGCGAC	330	0.0	41.81818	8
ATTGCGG	375	0.0	41.706665	1
ATAGCGG	265	0.0	41.660378	1
CCCGCAC	1045	0.0	41.598083	32
CCGCACT	1035	0.0	41.555557	33
CTAGCGG	375	0.0	41.09333	1
CGCACTT	1050	0.0	40.961903	34
CAACGCA	1170	0.0	40.692307	16
TCAACGC	1165	0.0	40.669533	15
CATGCGG	215	0.0	40.651165	1
TATGCGG	375	0.0	40.48	1
ACCCGCA	1085	0.0	40.064514	31
CGACGGT	230	0.0	40.0	27
CGTTTTC	955	0.0	39.979057	1
ATGACGG	190	0.0	39.94737	1
TACGGGA	710	0.0	39.84507	3
CGTTTCT	855	0.0	39.54386	1
ACGCAAG	1205	0.0	39.510372	18
>>END_MODULE