FastQCFastQC Report
Sat 14 Jan 2017
SRR2933065.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933065.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2689137
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT459271.7078713356738613No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC187770.6982537520401526No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT162930.6058821101342178No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAAT147450.5483171738739975No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC137060.5096802431412011No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC131580.4893019582118724No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGT122330.4549043057307976No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCC108000.40161583437362985No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTTA76260.2835854030493798No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC57460.21367449854730347No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC56580.210402073230185No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT54490.20263006310202863Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTT48660.1809502453761188Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCTATCGTTC47970.1783843664342873No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT44450.1652946651658134Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG40710.15138685756805995No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTAT39410.14655259289504402No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGC37770.14045398207677778No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCT36220.13469005112048957No Hit
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC35760.13297946515926856No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCCCTA33080.12301344260258962No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTT31890.1185882310942135No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTAT30960.11512987252044057No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC30530.11353084651321223No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA30020.11163432729533675No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT29330.10906844835350522No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCG27820.10345326400254058No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATTG206.3129707E-446.036
CGTTTAG206.3129707E-446.024
CGTTTTT129500.044.4548261
ACTTGAC48200.044.186726
TGCATTA36900.043.75609215
TCAACTT48700.043.7330633
CGGTCTA8150.043.4601230
TACGGGT2700.043.4444473
GCATTAG37550.043.36617716
CTTGACC49250.043.244677
CTCAACT49900.043.234472
CGACGGT8150.043.17791427
AACTTGA49500.042.97985
CATTAGA37050.042.95816417
TACGGGA10450.042.918663
CCTGCAT39500.042.5645613
CTTGCGG5900.042.4915241
GCTCAAC53600.042.438431
ATTAGAA37400.042.4331618
CCCTGCA40400.041.8440612
Produced by FastQC (version 0.11.5)