##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933065.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2689137
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.402356220601625	33.0	31.0	34.0	31.0	34.0
2	32.74450688083203	34.0	31.0	34.0	31.0	34.0
3	32.809333998230656	34.0	31.0	34.0	31.0	34.0
4	36.275648284189316	37.0	37.0	37.0	35.0	37.0
5	36.24564423456298	37.0	37.0	37.0	35.0	37.0
6	36.38052951560296	37.0	37.0	37.0	35.0	37.0
7	36.43310400325458	37.0	37.0	37.0	35.0	37.0
8	36.24664864601543	37.0	37.0	37.0	35.0	37.0
9	37.93007533643693	39.0	38.0	39.0	35.0	39.0
10	37.84691371246612	39.0	38.0	39.0	35.0	39.0
11	37.51572158651642	39.0	37.0	39.0	35.0	39.0
12	37.38311435973697	39.0	37.0	39.0	35.0	39.0
13	37.16614066148359	39.0	37.0	39.0	33.0	39.0
14	38.69038803155064	41.0	38.0	41.0	34.0	41.0
15	38.77431830360447	41.0	38.0	41.0	35.0	41.0
16	38.816222453523196	41.0	38.0	41.0	35.0	41.0
17	38.769239350765694	41.0	38.0	41.0	35.0	41.0
18	38.665633621492695	40.0	38.0	41.0	35.0	41.0
19	38.51971915153449	40.0	37.0	41.0	34.0	41.0
20	38.32496373371829	40.0	36.0	41.0	34.0	41.0
21	38.35872475072858	40.0	36.0	41.0	34.0	41.0
22	38.30700815912317	40.0	35.0	41.0	34.0	41.0
23	38.20201313655645	40.0	35.0	41.0	34.0	41.0
24	38.1399489873517	40.0	35.0	41.0	34.0	41.0
25	38.113145964671936	40.0	35.0	41.0	34.0	41.0
26	38.01207487755365	40.0	35.0	41.0	34.0	41.0
27	37.904715155828804	40.0	35.0	41.0	34.0	41.0
28	37.90090501153344	40.0	35.0	41.0	34.0	41.0
29	37.86842172786288	40.0	35.0	41.0	34.0	41.0
30	37.79264091044822	40.0	35.0	41.0	34.0	41.0
31	37.74700061767028	40.0	35.0	41.0	34.0	41.0
32	37.573425600852616	40.0	35.0	41.0	33.0	41.0
33	37.33635660808653	40.0	35.0	41.0	33.0	41.0
34	37.19779654216204	40.0	35.0	41.0	33.0	41.0
35	37.020911913375926	40.0	35.0	41.0	32.0	41.0
36	36.88519142014706	40.0	35.0	41.0	32.0	41.0
37	36.77978251015102	40.0	35.0	41.0	32.0	41.0
38	36.72294457292433	39.0	35.0	41.0	32.0	41.0
39	36.597482389331596	39.0	35.0	41.0	31.0	41.0
40	36.524421031728764	39.0	35.0	41.0	31.0	41.0
41	36.46404590022747	39.0	35.0	41.0	31.0	41.0
42	36.41162127478072	39.0	35.0	41.0	31.0	41.0
43	36.30576426563615	39.0	35.0	41.0	31.0	41.0
44	36.265133014792475	39.0	35.0	41.0	31.0	41.0
45	36.225495019405855	38.0	35.0	41.0	31.0	41.0
46	36.14614502719646	38.0	35.0	41.0	31.0	41.0
47	35.983434834298144	38.0	35.0	41.0	31.0	41.0
48	35.93990711518231	38.0	35.0	41.0	31.0	41.0
49	35.90096190710997	38.0	35.0	41.0	31.0	41.0
50	35.781618415127234	37.0	35.0	41.0	30.0	41.0
51	35.71912847876475	37.0	35.0	40.0	30.0	41.0
52	35.42802430668278	37.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	3.0
12	6.0
13	11.0
14	27.0
15	31.0
16	89.0
17	202.0
18	493.0
19	1042.0
20	2009.0
21	3890.0
22	6622.0
23	10007.0
24	13956.0
25	20682.0
26	28170.0
27	30169.0
28	29990.0
29	31350.0
30	35050.0
31	42365.0
32	53558.0
33	72526.0
34	162802.0
35	250171.0
36	193496.0
37	234547.0
38	367807.0
39	1083275.0
40	14787.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.712967766238755	19.1760404918009	23.013405415938273	21.097586326022068
2	28.315031922880834	25.237576218690233	23.75397757719298	22.69341428123595
3	25.50186174969888	25.544849518637392	28.862456617122888	20.090832114540834
4	21.6895234419072	25.389818369238903	28.36322582300567	24.557432365848225
5	22.87436452661207	36.33831225408002	22.887491414531873	17.89983180477603
6	80.1180824926361	6.770276114604797	10.638022532879507	2.4736188598795823
7	82.34809159964702	2.0393531456374294	10.477339012478724	5.1352162422368215
8	74.54372908483279	6.298265949261789	14.163503012304691	4.994501953600727
9	36.51807252661355	28.501522979305253	18.256526164341942	16.723878329739243
10	33.352447272117416	19.946956960541616	27.61179515956234	19.08880060777863
11	29.0953938010596	18.92867488714781	31.67659364323945	20.299337668553147
12	23.539336225711075	20.862380756354177	33.3186074194063	22.27967559852845
13	19.507336368507815	25.617103182173313	34.452354045182524	20.423206404136344
14	16.834359870843326	26.138497220483746	35.24491314499782	21.78222976367511
15	19.948072560081542	18.891004809349617	39.609845091566555	21.55107753900229
16	22.077566148545053	21.870771180493964	29.373252459804018	26.67841021115696
17	21.238374987960825	26.154115614042723	29.762894192449103	22.844615205547356
18	22.145022734059292	31.381703498185477	26.979622086937184	19.493651680818047
19	21.177611999686142	26.987059417203362	27.22330621310852	24.612022370001974
20	22.92185188036162	26.227745183677886	31.90220505686397	18.94819787909653
21	18.904726683690715	25.812928088081787	30.08076568802556	25.20157954020193
22	19.051874263007054	27.37975045525758	29.122242563320498	24.446132718414866
23	18.087252527483724	31.364002652151974	30.610452349582783	19.93829247078152
24	24.678660849186933	26.46049643435794	26.625344859707777	22.23549785674735
25	18.0419591861627	28.523946530057785	25.70289278679368	27.731201496985836
26	16.404110314944905	24.056788478980433	33.74238649797314	25.79671470810152
27	20.318600353942546	23.2632253395792	32.2758937160881	24.14228059039015
28	18.738688285498284	28.872273893074247	27.675904946456797	24.71313287497067
29	20.12601068669986	24.31081049422175	30.385696228938876	25.177482590139512
30	23.14262902931312	26.17460545892604	26.455104369914956	24.22766114184588
31	23.795403506775596	24.893934373741462	26.670601014377475	24.640061105105467
32	22.421691419961125	26.943662595100214	23.65480077809349	26.97984520684517
33	17.89183667473989	24.04544655032451	29.915359462905755	28.147357312029847
34	18.727383543493694	25.774253970697664	27.482980599352135	28.01538188645651
35	27.527753327554528	25.90013078545273	25.052684188273037	21.5194316987197
36	18.525125346904975	27.350819240522146	28.582403945949945	25.541651466622934
37	21.579785633829736	27.511093707758288	27.344980936263198	23.564139722148777
38	19.588663574968475	24.177533535851836	27.130711451294598	29.10309143788509
39	23.49132825884289	23.181526266605236	30.805645082418636	22.52150039213324
40	19.45222575123543	21.26012917898939	33.64633337758545	25.64131169218972
41	18.810495709218237	27.194821238189054	27.99533828138916	25.999344771203546
42	19.916910146266257	21.87475015218637	30.724355062609305	27.483984638938068
43	22.271829215097632	24.052809507288025	31.133371040597783	22.54199023701656
44	21.13986754858529	24.923237454990208	27.37714738966442	26.559747606760087
45	22.038445791344955	23.30230851012797	28.926380470760694	25.732865227766382
46	24.40039313727787	24.39615385902615	28.45864677032074	22.744806233375243
47	19.36115564212608	26.78178910185684	30.635888019093116	23.221167236923964
48	19.969789564458786	24.918514750271186	28.35898654475395	26.752709140516085
49	19.18262252908647	23.40449742798526	32.098550575891075	25.314329467037194
50	18.708269604709614	23.853005629687146	32.70469299258461	24.734031773018632
51	18.879811627299016	23.01876029372992	29.59012501036578	28.511303068605283
52	18.837902271249103	22.311990798534996	32.47960219207872	26.37050473813718
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2639.0
1	3366.0
2	4093.0
3	30250.5
4	56408.0
5	33101.0
6	9794.0
7	10603.0
8	11412.0
9	13607.5
10	15803.0
11	18127.5
12	20452.0
13	20980.0
14	19668.5
15	17829.0
16	15772.5
17	13716.0
18	12605.0
19	11494.0
20	10806.5
21	10119.0
22	9809.0
23	9499.0
24	10003.0
25	10507.0
26	12381.0
27	14255.0
28	15767.5
29	17280.0
30	26506.5
31	35733.0
32	32547.0
33	29361.0
34	34008.5
35	38656.0
36	44016.0
37	49376.0
38	55639.5
39	66711.5
40	71520.0
41	79053.5
42	86587.0
43	104845.5
44	123104.0
45	166325.5
46	209547.0
47	229818.0
48	250089.0
49	272733.0
50	295377.0
51	267787.0
52	240197.0
53	206281.0
54	172365.0
55	161538.0
56	150711.0
57	140933.0
58	131155.0
59	124297.5
60	117440.0
61	107554.0
62	97668.0
63	94085.0
64	79211.5
65	67921.0
66	58212.5
67	48504.0
68	39000.0
69	29496.0
70	23797.0
71	18098.0
72	15400.0
73	12702.0
74	9809.0
75	6916.0
76	5497.0
77	4078.0
78	2845.0
79	1612.0
80	1309.5
81	1007.0
82	696.0
83	385.0
84	292.5
85	200.0
86	132.5
87	65.0
88	52.5
89	22.5
90	5.0
91	4.0
92	3.0
93	3.0
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2689137.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.2496125205078
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.91493038112972	16.000793354194307
2	11.277227043803999	5.018278640608612
3	4.528958021064504	3.0230268327099306
4	2.330692321121878	2.0742800419793888
5	1.4109151392167285	1.5696157573445262
6	0.9189353985295396	1.2267573929196405
7	0.6283679325854045	0.9786660114236472
8	0.47371389569792555	0.8431960499887272
9	0.40803158683117735	0.8170690232809465
>10	4.3188908512794635	22.778713450194456
>50	1.3721011870925968	21.541552140606214
>100	0.39936727598452687	12.597851915043798
>500	0.008260182240245276	1.301728950135756
>1k	0.007585881649204845	3.8478006551180473
>5k	6.743005910404306E-4	0.9181416173103548
>10k+	0.0013486011820808613	5.462528167141745
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45927	1.7078713356738613	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	18777	0.6982537520401526	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT	16293	0.6058821101342178	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAAT	14745	0.5483171738739975	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	13706	0.5096802431412011	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	13158	0.4893019582118724	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGT	12233	0.4549043057307976	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCC	10800	0.40161583437362985	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTTA	7626	0.2835854030493798	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5746	0.21367449854730347	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5658	0.210402073230185	No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	5449	0.20263006310202863	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCTT	4866	0.1809502453761188	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCTATCGTTC	4797	0.1783843664342873	No Hit
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	4445	0.1652946651658134	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG	4071	0.15138685756805995	No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTAT	3941	0.14655259289504402	No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGC	3777	0.14045398207677778	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCT	3622	0.13469005112048957	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	3576	0.13297946515926856	No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCCCTA	3308	0.12301344260258962	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTT	3189	0.1185882310942135	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTATCGTTCGTAT	3096	0.11512987252044057	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTC	3053	0.11353084651321223	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	3002	0.11163432729533675	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCT	2933	0.10906844835350522	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCG	2782	0.10345326400254058	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18693729624039238	0.0
2	0.0	0.0	0.0	0.6500226652639862	0.0
3	0.0	0.0	0.0	1.2221764826410852	0.0
4	0.0	0.0	0.0	1.8116964661897106	0.0
5	0.0	0.0	0.0	4.571094741547196	0.0
6	0.0	0.0	0.0	5.361906068749938	0.0
7	0.0	0.0	0.0	6.92512133074663	0.0
8	0.0	0.0	0.0	8.86566954379788	0.0
9	0.0	0.0	0.0	10.314610226254743	0.0
10	0.0	0.0	0.0	11.878494847975391	0.0
11	0.0	0.0	0.0	12.892612016420138	0.0
12	0.0	0.0	0.0	14.039076476951527	0.0
13	0.0	0.0	0.0	14.571105897542594	0.0
14	0.0	0.0	0.0	14.801291269280814	0.0
15	0.0	0.0	0.0	15.564398541242042	0.0
16	0.0	0.0	0.0	16.300657050942366	0.0
17	0.0	0.0	0.0	17.093104590803666	0.0
18	0.0	0.0	0.0	17.49650538444118	0.0
19	3.718665133089166E-5	0.0	0.0	18.119865220700916	0.0
20	3.718665133089166E-5	3.718665133089166E-5	0.0	18.69350650413125	0.0
21	3.718665133089166E-5	3.718665133089166E-5	0.0	19.241117131629963	0.0
22	3.718665133089166E-5	3.718665133089166E-5	0.0	19.838855365122715	0.0
23	3.718665133089166E-5	3.718665133089166E-5	0.0	20.291082231957688	0.0
24	3.718665133089166E-5	3.718665133089166E-5	0.0	20.62784454641024	0.0
25	7.437330266178332E-5	3.718665133089166E-5	0.0	21.08025734650187	0.0
26	7.437330266178332E-5	3.718665133089166E-5	0.0	21.474510223911984	0.0
27	1.1155995399267497E-4	3.718665133089166E-5	0.0	21.8839352550651	0.0
28	1.1155995399267497E-4	3.718665133089166E-5	0.0	22.297078951351306	0.0
29	1.1155995399267497E-4	3.718665133089166E-5	0.0	22.689769989405523	0.0
30	1.1155995399267497E-4	3.718665133089166E-5	0.0	23.053046386257005	0.0
31	1.1155995399267497E-4	3.718665133089166E-5	0.0	23.36526551083117	0.0
32	1.4874660532356664E-4	3.718665133089166E-5	0.0	23.788300856371393	0.0
33	1.859332566544583E-4	3.718665133089166E-5	0.0	24.16972433907235	0.0
34	1.859332566544583E-4	3.718665133089166E-5	0.0	24.73607703884183	0.0
35	1.859332566544583E-4	3.718665133089166E-5	0.0	25.07607459196017	0.0
36	1.859332566544583E-4	3.718665133089166E-5	0.0	25.462890139104108	0.0
37	2.2311990798534994E-4	3.718665133089166E-5	0.0	25.96260435968863	0.0
38	2.603065593162416E-4	3.718665133089166E-5	0.0	26.98282013895164	0.0
39	2.603065593162416E-4	3.718665133089166E-5	0.0	27.46643253950989	0.0
40	2.603065593162416E-4	3.718665133089166E-5	0.0	27.884670807028424	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATTG	20	6.3129707E-4	46.0	36
CGTTTAG	20	6.3129707E-4	46.0	24
CGTTTTT	12950	0.0	44.454826	1
ACTTGAC	4820	0.0	44.18672	6
TGCATTA	3690	0.0	43.756092	15
TCAACTT	4870	0.0	43.733063	3
CGGTCTA	815	0.0	43.46012	30
TACGGGT	270	0.0	43.444447	3
GCATTAG	3755	0.0	43.366177	16
CTTGACC	4925	0.0	43.24467	7
CTCAACT	4990	0.0	43.23447	2
CGACGGT	815	0.0	43.177914	27
AACTTGA	4950	0.0	42.9798	5
CATTAGA	3705	0.0	42.958164	17
TACGGGA	1045	0.0	42.91866	3
CCTGCAT	3950	0.0	42.56456	13
CTTGCGG	590	0.0	42.491524	1
GCTCAAC	5360	0.0	42.43843	1
ATTAGAA	3740	0.0	42.43316	18
CCCTGCA	4040	0.0	41.84406	12
>>END_MODULE