##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933064.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2692719
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50878684333568	33.0	31.0	34.0	31.0	34.0
2	32.88117735270557	34.0	31.0	34.0	31.0	34.0
3	32.93408372726601	34.0	31.0	34.0	31.0	34.0
4	36.390841747690715	37.0	37.0	37.0	35.0	37.0
5	36.37149624598779	37.0	37.0	37.0	35.0	37.0
6	36.4916227797999	37.0	37.0	37.0	35.0	37.0
7	36.524362920898916	37.0	37.0	37.0	35.0	37.0
8	36.378178339440545	37.0	37.0	37.0	35.0	37.0
9	38.06190805650348	39.0	39.0	39.0	35.0	39.0
10	37.97046702608033	39.0	38.0	39.0	35.0	39.0
11	37.6842615215327	39.0	37.0	39.0	35.0	39.0
12	37.59320968879411	39.0	37.0	39.0	35.0	39.0
13	37.50392744285609	39.0	37.0	39.0	35.0	39.0
14	39.01255979550781	41.0	38.0	41.0	35.0	41.0
15	39.06315103803999	41.0	38.0	41.0	35.0	41.0
16	39.04228996787262	41.0	38.0	41.0	35.0	41.0
17	39.02137133507061	41.0	38.0	41.0	35.0	41.0
18	38.89668435510723	40.0	38.0	41.0	35.0	41.0
19	38.751698933308674	40.0	37.0	41.0	35.0	41.0
20	38.57873212912302	40.0	36.0	41.0	35.0	41.0
21	38.52696735158774	40.0	36.0	41.0	35.0	41.0
22	38.473899801650305	40.0	36.0	41.0	35.0	41.0
23	38.40407446896613	40.0	36.0	41.0	35.0	41.0
24	38.33416632036243	40.0	35.0	41.0	35.0	41.0
25	38.269491543677596	40.0	35.0	41.0	35.0	41.0
26	38.154212897818155	40.0	35.0	41.0	34.0	41.0
27	38.06236001602841	40.0	35.0	41.0	34.0	41.0
28	38.0403759174277	40.0	35.0	41.0	34.0	41.0
29	37.99472874815382	40.0	35.0	41.0	34.0	41.0
30	37.886519536572514	40.0	35.0	41.0	34.0	41.0
31	37.78245260645467	40.0	35.0	41.0	34.0	41.0
32	37.60680078389167	40.0	35.0	41.0	34.0	41.0
33	37.33621406466846	40.0	35.0	41.0	33.0	41.0
34	37.13198443654908	40.0	35.0	41.0	33.0	41.0
35	36.97054018633211	40.0	35.0	41.0	32.0	41.0
36	36.86497068576409	40.0	35.0	41.0	32.0	41.0
37	36.725584065771436	39.0	35.0	41.0	32.0	41.0
38	36.69405719646201	39.0	35.0	41.0	32.0	41.0
39	36.59281677739118	39.0	35.0	41.0	31.0	41.0
40	36.52771306623528	39.0	35.0	41.0	31.0	41.0
41	36.46670112997309	39.0	35.0	41.0	31.0	41.0
42	36.39600307347332	39.0	35.0	41.0	31.0	41.0
43	36.2892782351222	39.0	35.0	41.0	31.0	41.0
44	36.17921402121796	39.0	35.0	41.0	31.0	41.0
45	36.09815840420036	38.0	35.0	41.0	31.0	41.0
46	36.012897001135286	38.0	35.0	41.0	31.0	41.0
47	35.88140203266661	38.0	35.0	41.0	31.0	41.0
48	35.792646391992626	37.0	35.0	41.0	30.0	41.0
49	35.7147236677871	37.0	35.0	40.0	30.0	41.0
50	35.59383507896665	37.0	35.0	40.0	30.0	41.0
51	35.46714974715149	37.0	35.0	40.0	29.0	41.0
52	35.18304694994168	36.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	2.0
11	3.0
12	9.0
13	13.0
14	32.0
15	61.0
16	106.0
17	222.0
18	490.0
19	923.0
20	1703.0
21	2999.0
22	4650.0
23	7297.0
24	11798.0
25	19953.0
26	28513.0
27	32291.0
28	32561.0
29	32127.0
30	34070.0
31	39971.0
32	49213.0
33	65399.0
34	147001.0
35	247321.0
36	203857.0
37	245912.0
38	377935.0
39	1094264.0
40	12019.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.48652421585765	20.506632886684425	22.585089643590734	23.421753253867188
2	31.36662236200658	23.152880044297234	24.80619032286696	20.674307270829225
3	27.038283608501295	23.95968535892531	28.841182462782044	20.16084856979135
4	23.634623590504617	26.533366459701142	26.488319055943084	23.34369089385116
5	22.339687134082688	36.51732691008605	24.024638293115622	17.118347662715642
6	84.54673510306868	2.990694535894759	10.149777975347595	2.3127923856889634
7	85.54227158496673	2.202272127169601	9.026192484251048	3.229263803612631
8	78.9065253374006	4.640996702589464	12.251853981050381	4.200623978959557
9	38.213419224211655	29.244603688687903	17.058742482969816	15.483234604130619
10	30.865901714958007	21.22237782702168	28.450759251150977	19.46096120686934
11	28.072071389550857	19.445252178188664	32.706160575982864	19.776515856277612
12	22.74217993039749	21.972326113493462	34.95916209600779	20.326331860101256
13	18.85172570921808	26.514686456329084	34.33737423028545	20.296213604167388
14	18.1727837178703	25.050478716865744	36.585473642069594	20.191263923194363
15	21.288704837006758	21.096817009127207	36.7058723914378	20.908605762428238
16	21.678608128066834	23.16372410192077	30.745540102773443	24.412127667238952
17	22.18437943209076	25.88810789391689	30.959821652389273	20.96769102160307
18	23.620585735087843	29.067125088061545	27.680422650859597	19.631866525991015
19	23.402924701760565	26.19222429076335	27.38934140547157	23.015509602004517
20	24.40585148320341	26.70416036727189	29.27847280016964	19.61151534935506
21	21.06432197344023	24.113953219775254	29.50166727385962	25.3200575329249
22	20.267580835579206	28.28096804753857	28.480506135248422	22.970944981633806
23	19.646090067326	28.216460759551964	30.785165477719733	21.352283695402306
24	24.56301604437745	25.572478970141333	28.15952945702838	21.704975528452838
25	20.60686614533488	26.444162944592435	28.10694320499094	24.84202770508174
26	19.393482944191355	24.899144693523535	31.957066444734856	23.75030591755025
27	21.628472930149787	24.903564018376965	31.799047728337044	21.668915323136208
28	21.385818572231265	27.30310886505424	28.5225825643151	22.788489998399385
29	23.873081446671563	24.50404219675354	29.86401477465714	21.758861581917756
30	26.645929263320827	23.879506179441673	27.761010339363295	21.7135542178742
31	26.229807120609316	25.567651136267838	26.347717678673487	21.854824064449353
32	26.418686836613848	23.98204194347795	26.364949331883498	23.234321888024706
33	22.076495913609996	24.753344110544028	29.509354670873567	23.66080530497241
34	22.30180720676758	25.20831174734534	29.2153395879778	23.274541457909272
35	27.573021915766184	25.590304818289617	26.867304015012333	19.969369250931866
36	23.805046126238942	27.448686624931902	26.914988158808995	21.83127909002016
37	23.865542598392185	27.897155254595816	28.475492615456723	19.76180953155528
38	22.274362828055956	26.139415215624055	26.652353996090937	24.933867960229048
39	24.846112795282387	25.227994454675738	29.59948661557333	20.32640613446854
40	20.996695162027677	24.94638319111649	31.157094371896953	22.89982727495888
41	21.781181029286753	28.154738760338528	27.388598661798724	22.675481548575995
42	21.636717384918366	24.8657212282455	28.499037589885912	24.99852379695022
43	23.18920020989936	25.58425145735593	30.011672216818763	21.214876115925946
44	23.509471281630205	25.921531359194926	28.134239034968	22.43475832420687
45	23.632283947935154	25.69027811665458	28.201345925809562	22.476092009600706
46	24.77740900554421	25.05887172036889	28.226896308155435	21.936822965931462
47	22.042738213679183	27.24877716538562	28.498740492416776	22.209744128518423
48	21.944398951394483	26.17818643534658	27.931432875097624	23.945981738161315
49	20.866009412790564	24.933607999943554	30.966580619812166	23.233801967453715
50	21.094663052475955	25.721101979077655	30.875445971154065	22.30878899729233
51	20.833625788654516	24.206016298024412	29.850645388545928	25.109712524775148
52	20.71842624499623	23.817301396840886	30.077776403701982	25.3864959544609
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3073.0
1	3745.5
2	4418.0
3	33910.5
4	63403.0
5	37791.5
6	12180.0
7	13029.0
8	13878.0
9	16367.0
10	18856.0
11	21011.5
12	23167.0
13	23555.5
14	22426.0
15	20908.0
16	18739.5
17	16571.0
18	15070.0
19	13569.0
20	13331.5
21	13094.0
22	13266.0
23	13438.0
24	12741.0
25	12044.0
26	12949.5
27	13855.0
28	16667.5
29	19480.0
30	22593.0
31	25706.0
32	29177.0
33	32648.0
34	37397.0
35	42146.0
36	48908.0
37	55670.0
38	59302.5
39	69515.5
40	76096.0
41	84578.5
42	93061.0
43	108769.0
44	124477.0
45	147041.0
46	169605.0
47	187791.0
48	205977.0
49	231540.5
50	257104.0
51	243780.0
52	230456.0
53	203035.5
54	175615.0
55	163559.0
56	151503.0
57	144192.0
58	136881.0
59	133314.5
60	129748.0
61	122976.0
62	116204.0
63	105375.0
64	81749.5
65	68953.0
66	60746.0
67	52539.0
68	44841.5
69	37144.0
70	30900.5
71	24657.0
72	21232.5
73	17808.0
74	15097.5
75	12387.0
76	9748.0
77	7109.0
78	5137.0
79	3165.0
80	2350.0
81	1535.0
82	1151.0
83	767.0
84	505.0
85	243.0
86	166.0
87	89.0
88	52.0
89	17.5
90	20.0
91	14.5
92	9.0
93	8.5
94	8.0
95	8.0
96	8.0
97	7.5
98	7.0
99	3.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2692719.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.425773834778457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.27519696036343	15.914035716063712
2	9.40375247547474	4.029653474755196
3	3.7136215240405446	2.38701644646174
4	1.9075261060804154	1.634808917312584
5	1.1949652936493795	1.2801528061070617
6	0.8503467380000087	1.0931602133717877
7	0.6351732635658429	0.9526355083741914
8	0.48111520426400184	0.8246612444026994
9	0.39402711562063	0.7598102277651949
>10	5.044845266536604	25.943811642989022
>50	1.5792335114738079	23.98307287911124
>100	0.5095306063818041	14.434087993376139
>500	0.004720987423248089	0.6769236904962794
>1k	0.004546136037201863	1.8997995057145844
>5k	6.994055441849021E-4	0.8697471026931
>10k+	6.994055441849021E-4	3.316622631005347
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	50173	1.8632839148830607	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	16667	0.6189654397655306	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	11449	0.4251836155202232	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	10241	0.3803218976803744	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC	6676	0.24792783799572105	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC	5685	0.2111248890062424	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTT	5488	0.20380886382871735	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT	5367	0.19931526460800403	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4738	0.17595597609702313	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4493	0.16685736610466967	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCT	3148	0.11690785410583132	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10205298064892772	0.0
2	3.713718364225899E-5	0.0	0.0	0.2957976677105929	0.0
3	3.713718364225899E-5	0.0	0.0	0.6858495075052391	0.0
4	3.713718364225899E-5	0.0	0.0	0.965863872167872	0.0
5	3.713718364225899E-5	0.0	0.0	2.42156719657714	0.0
6	3.713718364225899E-5	0.0	0.0	3.2702261171700426	0.0
7	3.713718364225899E-5	0.0	0.0	4.839792046626477	0.0
8	3.713718364225899E-5	0.0	0.0	6.386704294061133	0.0
9	3.713718364225899E-5	0.0	0.0	7.97769837847915	0.0
10	3.713718364225899E-5	0.0	0.0	8.853838814967325	0.0
11	3.713718364225899E-5	0.0	0.0	9.463854193475072	0.0
12	3.713718364225899E-5	0.0	0.0	9.95655320885692	0.0
13	3.713718364225899E-5	0.0	0.0	10.238387295518026	0.0
14	7.427436728451799E-5	0.0	0.0	10.38117976662251	0.0
15	7.427436728451799E-5	0.0	0.0	10.669438586053724	0.0
16	7.427436728451799E-5	0.0	0.0	11.017562545516261	0.0
17	7.427436728451799E-5	0.0	0.0	11.45336739555817	0.0
18	7.427436728451799E-5	0.0	0.0	11.68187248650899	0.0
19	7.427436728451799E-5	0.0	0.0	11.944395237676119	0.0
20	7.427436728451799E-5	0.0	0.0	12.24100992342684	0.0
21	2.9709746913807194E-4	0.0	0.0	12.556787395936968	0.0
22	2.9709746913807194E-4	0.0	0.0	12.88938801263704	0.0
23	2.9709746913807194E-4	0.0	0.0	13.143183525648238	0.0
24	2.9709746913807194E-4	0.0	0.0	13.343761454500079	0.0
25	2.9709746913807194E-4	0.0	0.0	13.548795845388991	0.0
26	2.9709746913807194E-4	0.0	0.0	13.776669604217894	0.0
27	2.9709746913807194E-4	0.0	0.0	14.016761496465097	0.0
28	2.9709746913807194E-4	0.0	0.0	14.26275820091142	0.0
29	2.9709746913807194E-4	0.0	0.0	14.52628365603689	0.0
30	2.9709746913807194E-4	0.0	0.0	14.761807674696097	0.0
31	2.9709746913807194E-4	0.0	0.0	14.987304653771893	0.0
32	3.3423465278033097E-4	0.0	0.0	15.243959729923546	0.0
33	3.3423465278033097E-4	0.0	0.0	15.499797788035067	0.0
34	3.3423465278033097E-4	0.0	0.0	15.820663054704186	0.0
35	3.3423465278033097E-4	0.0	0.0	16.065954152661305	0.0
36	3.3423465278033097E-4	0.0	0.0	16.330630860479687	0.0
37	3.3423465278033097E-4	0.0	0.0	16.64989922825219	0.0
38	3.3423465278033097E-4	0.0	0.0	17.20562004427495	0.0
39	3.3423465278033097E-4	0.0	0.0	17.54460825656149	0.0
40	3.3423465278033097E-4	0.0	0.0	17.887755833415962	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15500	0.0	43.90774	1
CGCACTT	6770	0.0	41.583458	34
CCGCACT	6830	0.0	41.319183	33
CACTTAC	6850	0.0	41.164963	36
ACCCGCA	6940	0.0	41.028816	31
ACTTACT	6875	0.0	40.948364	37
GCACTTA	6875	0.0	40.948364	35
TATGACC	7180	0.0	40.938717	27
CCCGCAC	6990	0.0	40.932762	32
TACGGGA	1125	0.0	40.88889	3
TTATGAC	7340	0.0	40.57902	26
CTTATGA	7325	0.0	40.567917	25
CAACGCA	7475	0.0	40.553844	16
CGTAGGG	1140	0.0	40.55263	2
CGCAAGC	7465	0.0	40.546547	19
ATCAACG	7495	0.0	40.507004	14
AACGCAA	7490	0.0	40.441925	17
TCAACGC	7520	0.0	40.43351	15
CTTACTG	6990	0.0	40.406296	38
GGACTTA	7890	0.0	40.37389	7
>>END_MODULE