##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933062.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1070272
Sequences flagged as poor quality	0
Sequence length	52
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4119952684925	33.0	31.0	34.0	31.0	34.0
2	32.786115118399806	34.0	31.0	34.0	31.0	34.0
3	32.830777596723074	34.0	31.0	34.0	31.0	34.0
4	36.291684730610534	37.0	37.0	37.0	35.0	37.0
5	36.16878513125636	37.0	35.0	37.0	35.0	37.0
6	36.26244263140585	37.0	37.0	37.0	35.0	37.0
7	36.52005378072116	37.0	37.0	37.0	35.0	37.0
8	36.4817868728697	37.0	37.0	37.0	35.0	37.0
9	38.304279659750044	39.0	39.0	39.0	37.0	39.0
10	37.96744285564791	39.0	38.0	39.0	35.0	39.0
11	37.6604685537882	39.0	37.0	39.0	35.0	39.0
12	37.32998994648089	39.0	37.0	39.0	35.0	39.0
13	37.25552476379836	39.0	37.0	39.0	34.0	39.0
14	38.07704396639359	40.0	37.0	41.0	33.0	41.0
15	38.38270738653352	40.0	37.0	41.0	33.0	41.0
16	38.598374992525265	41.0	37.0	41.0	34.0	41.0
17	38.6717787627818	41.0	37.0	41.0	35.0	41.0
18	38.67929834658853	40.0	37.0	41.0	35.0	41.0
19	38.644046560126775	40.0	37.0	41.0	35.0	41.0
20	38.50813718381869	40.0	36.0	41.0	34.0	41.0
21	38.46351861956587	40.0	36.0	41.0	35.0	41.0
22	38.41748172427196	40.0	36.0	41.0	35.0	41.0
23	38.40570995036776	40.0	36.0	41.0	35.0	41.0
24	38.36720758835137	40.0	35.0	41.0	34.0	41.0
25	38.20943647969862	40.0	35.0	41.0	34.0	41.0
26	38.118534353883874	40.0	35.0	41.0	34.0	41.0
27	38.04524550768403	40.0	35.0	41.0	34.0	41.0
28	38.01226604078216	40.0	35.0	41.0	34.0	41.0
29	37.98635954224721	40.0	35.0	41.0	34.0	41.0
30	37.93811199396041	40.0	35.0	41.0	34.0	41.0
31	37.80189241613347	40.0	35.0	41.0	34.0	41.0
32	37.726716199246546	40.0	35.0	41.0	34.0	41.0
33	37.621144905220355	40.0	35.0	41.0	34.0	41.0
34	37.48317997667883	40.0	35.0	41.0	33.0	41.0
35	37.4406113586079	40.0	35.0	41.0	33.0	41.0
36	37.369443468576215	40.0	35.0	41.0	33.0	41.0
37	37.23143929767386	40.0	35.0	41.0	33.0	41.0
38	37.24145544310232	40.0	35.0	41.0	33.0	41.0
39	37.20244479907911	40.0	35.0	41.0	33.0	41.0
40	37.119505135143214	40.0	35.0	41.0	33.0	41.0
41	37.00082408957723	40.0	35.0	41.0	33.0	41.0
42	37.04569399180769	40.0	35.0	41.0	33.0	41.0
43	36.99810982628715	39.0	35.0	41.0	33.0	41.0
44	36.963386877354544	39.0	35.0	41.0	32.0	41.0
45	36.89008121300006	39.0	35.0	41.0	32.0	41.0
46	36.808318819888775	39.0	35.0	41.0	32.0	41.0
47	36.70117035669438	39.0	35.0	41.0	32.0	41.0
48	36.59065265652096	39.0	35.0	41.0	32.0	41.0
49	36.49861904263589	39.0	35.0	41.0	32.0	41.0
50	36.3186993586677	38.0	35.0	40.0	31.0	41.0
51	36.19099163577109	38.0	35.0	40.0	31.0	41.0
52	35.94635195539078	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	1.0
13	2.0
14	6.0
15	9.0
16	41.0
17	79.0
18	162.0
19	318.0
20	553.0
21	857.0
22	1364.0
23	2198.0
24	3547.0
25	5478.0
26	7276.0
27	8567.0
28	9690.0
29	11222.0
30	13589.0
31	16871.0
32	21778.0
33	30038.0
34	70864.0
35	112262.0
36	68316.0
37	76046.0
38	123752.0
39	482415.0
40	2969.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.90447661902769	23.491037792262155	27.19803937690606	11.406446211804102
2	34.95634754529689	23.66734811337679	27.70379866052742	13.6725056807989
3	31.068924535071456	24.58907642169467	30.45590279854093	13.886096244692938
4	27.841146923398913	28.48574941697064	29.36048032649644	14.312623333134006
5	23.97474660647013	33.37488040423369	27.993631525444002	14.65674146385218
6	25.010184326974827	40.832517341386115	24.64616471326915	9.511133618369911
7	89.72195853016804	1.2161394486635173	6.923753961609759	2.138148059558692
8	87.73162336303295	1.5606313161514083	7.091468337020869	3.6162769837947737
9	83.00123706870777	3.748486366082641	9.271474914788017	3.9788016504215746
10	44.7538569634635	32.080816839083894	13.467417628415953	9.697908569036656
11	27.472922771033904	23.56186091012378	33.78813983734975	15.177076481492554
12	29.148571578066136	19.702094420857502	32.956482090533996	18.19285191054237
13	26.82505008072714	23.574007355139628	34.44031050050828	15.160632063624949
14	16.250728786700954	26.64219936614244	36.9337887938767	20.173283053279913
15	12.674254768881182	31.664287209232793	38.85619730311547	16.80526071877056
16	23.98184760509478	28.94451130179992	32.18144546433056	14.89219562877474
17	21.18807181725767	27.662874484243254	28.210025115110927	22.93902858338815
18	19.440385247862228	26.697138671291036	35.16694821503319	18.69552786581355
19	19.891485528912277	29.815972014590685	28.61908000956766	21.673462446929378
20	16.27632975542666	38.477601955390774	27.600460443700293	17.64560784548227
21	19.95829097649943	31.57234796388208	33.65527641571489	14.814084643903605
22	17.99000627877773	26.45570471805298	34.18121748490103	21.37307151826825
23	17.0861239012139	28.733910632063626	33.414403067631405	20.765562399091074
24	18.182293846797823	31.06331848352568	31.534320247563237	19.220067422113257
25	20.637464121270106	29.072422711236023	30.200453716438435	20.089659451055432
26	16.717058841117023	33.035714285714285	26.862704060276265	23.384522812892424
27	21.207786431860313	29.95425461938647	29.533146714106323	19.304812234646892
28	16.136085032589847	31.832842492375768	28.21553773246427	23.81553474257011
29	20.120399300364767	30.306781827423308	27.252978682054657	22.31984019015727
30	25.47137550080727	28.62814312623333	25.490809812832627	20.40967156012677
31	22.579213508341805	29.108862046283562	27.84983630329486	20.46208814207977
32	27.12787029839144	27.8613287089637	23.310336064103332	21.70046492854153
33	26.39413158524188	27.86160901154099	25.990869610715784	19.753389792501345
34	18.532952370986067	27.80237173354063	30.577927853853975	23.086748041619327
35	20.850587514201997	31.24121718591162	26.011612001435147	21.896583298451237
36	26.2988287089637	28.300656281767626	24.29447841296418	21.10603659630449
37	18.32141735932548	32.84267924415475	27.658856813968786	21.17704658255098
38	20.75005232314776	29.622469802069006	28.597683579501286	21.02979429528195
39	19.032544997907074	31.356234676792443	27.21560500508282	22.395615320217665
40	24.64364199007355	28.40362076182503	28.583855319021705	18.368881929079713
41	18.210324104526702	26.351432159301563	27.85516205226335	27.58308168390839
42	18.068023829456436	27.863197392812296	31.344181665969025	22.724597111762243
43	18.460914608622854	28.670655683788794	31.297744872331517	21.57068483525683
44	22.58724884889075	27.77135158165401	26.883539885188064	22.757859684267174
45	19.374327273814508	29.209210368952938	24.672700023919152	26.743762333313402
46	22.58463269150272	28.321118369909705	26.53736620223644	22.556882736351135
47	17.95870582431382	27.762942504335346	30.977639329067753	23.300712342283084
48	18.076713209352388	30.482624977575796	29.67843688333433	21.762224929737485
49	22.748516265024218	27.2468120253543	28.872193236859417	21.132478472762063
50	20.35146205824314	27.038360342043894	28.633562309394243	23.976615290318723
51	18.604429528194704	28.2115200621898	26.713115918196493	26.470934491419
52	19.080476738623453	28.822205943909584	29.303859205884113	22.79345811158285
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	720.0
1	1118.0
2	1516.0
3	8504.5
4	15493.0
5	8729.5
6	1966.0
7	2541.5
8	3117.0
9	4723.0
10	6329.0
11	8099.0
12	9869.0
13	10289.5
14	9857.0
15	9004.0
16	8108.5
17	7213.0
18	6244.0
19	5275.0
20	5213.0
21	5151.0
22	5391.5
23	5632.0
24	6665.5
25	7699.0
26	10005.5
27	12312.0
28	13281.0
29	14250.0
30	16382.0
31	18514.0
32	21165.0
33	23816.0
34	27520.0
35	31224.0
36	32877.0
37	34530.0
38	37427.5
39	42553.0
40	44781.0
41	50412.5
42	56044.0
43	58821.0
44	61598.0
45	64640.5
46	67683.0
47	76613.0
48	85543.0
49	101014.5
50	116486.0
51	110135.5
52	103785.0
53	95336.0
54	86887.0
55	73893.5
56	60900.0
57	51267.0
58	41634.0
59	41856.5
60	42079.0
61	29093.0
62	16107.0
63	12694.0
64	7321.5
65	5362.0
66	4587.0
67	3812.0
68	2801.0
69	1790.0
70	1189.0
71	588.0
72	509.0
73	430.0
74	322.5
75	215.0
76	280.5
77	346.0
78	275.0
79	204.0
80	120.0
81	36.0
82	23.0
83	10.0
84	5.5
85	1.0
86	1.0
87	1.0
88	0.5
89	1.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1070272.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.60727623446755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.04416349177544	14.702490367100637
2	13.062150213516702	5.644749757591294
3	5.611476029101303	3.637461379316548
4	3.0014487846087152	2.5941253197058964
5	1.7574030859122753	1.8986346966306122
6	1.077971046684773	1.3975210907085602
7	0.7475144456755728	1.1306225781876893
8	0.5976986697905977	1.03317122105114
9	0.4586851412625434	0.8919842896714993
>10	3.7921753213557237	17.252738879440095
>50	0.9469664800840754	14.800138696681712
>100	0.8889182139014087	28.821697115748098
>500	0.007364332181444421	0.997515007770015
>1k	0.004765156117405213	1.640091767490495
>5k	4.331960106732012E-4	0.7686584633393415
>10k+	8.663920213464024E-4	2.7883993695663647
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	15745	1.471121359803863	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14045	1.3122832326735634	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	8212	0.7672815882317766	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	2425	0.2265779166417509	No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCTT	2364	0.220878430903546	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT	2154	0.20125725049333254	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	1804	0.16855528314297674	No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGCT	1724	0.1610805477486097	No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1424	0.1330502900197333	No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCGGCAGACTTCG	1247	0.11651243795969622	No Hit
GGGGCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCT	1195	0.11165385995335765	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCG	1163	0.10866396579561083	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2286334688752018	0.0
2	0.0	0.0	0.0	0.7488750523231478	0.0
3	0.0	0.0	0.0	1.1470915804580517	0.0
4	0.0	0.0	0.0	1.6422928003348682	0.0
5	9.3434192429588E-5	0.0	0.0	3.1260277761167257	0.0
6	9.3434192429588E-5	0.0	0.0	4.5145533098128325	0.0
7	9.3434192429588E-5	0.0	0.0	5.668839323087963	0.0
8	9.3434192429588E-5	0.0	0.0	7.83520450876039	0.0
9	9.3434192429588E-5	0.0	0.0	8.929505770495725	0.0
10	9.3434192429588E-5	0.0	0.0	10.195632512109071	0.0
11	9.3434192429588E-5	0.0	0.0	12.149715212581475	0.0
12	9.3434192429588E-5	0.0	0.0	13.280829546134067	0.0
13	9.3434192429588E-5	0.0	0.0	13.735573760688872	0.0
14	9.3434192429588E-5	0.0	0.0	13.968224899838546	0.0
15	9.3434192429588E-5	0.0	0.0	14.266934013035938	0.0
16	9.3434192429588E-5	0.0	0.0	14.822587155414698	0.0
17	9.3434192429588E-5	0.0	0.0	15.527080966333791	0.0
18	9.3434192429588E-5	0.0	0.0	16.280721162470847	0.0
19	1.86868384859176E-4	0.0	0.0	16.729579022902588	0.0
20	1.86868384859176E-4	0.0	0.0	17.275234706691382	0.0
21	2.8030257728876395E-4	0.0	0.0	17.814910602164684	0.0
22	2.8030257728876395E-4	0.0	0.0	18.417748011720384	0.0
23	2.8030257728876395E-4	0.0	0.0	19.014045027806016	0.0
24	2.8030257728876395E-4	0.0	0.0	19.409084793398314	0.0
25	2.8030257728876395E-4	0.0	0.0	19.832341685104346	0.0
26	2.8030257728876395E-4	0.0	0.0	20.202995126472523	0.0
27	4.6717096214794E-4	0.0	0.0	20.697635741194762	0.0
28	5.606051545775279E-4	0.0	0.0	21.07389523410871	0.0
29	5.606051545775279E-4	0.0	0.0	21.571992913950847	0.0
30	5.606051545775279E-4	0.0	0.0	22.76383947258267	0.0
31	5.606051545775279E-4	0.0	0.0	23.306598696406148	0.0
32	5.606051545775279E-4	0.0	0.0	23.660807719906714	0.0
33	6.54039347007116E-4	0.0	0.0	24.078271691682115	0.0
34	6.54039347007116E-4	0.0	0.0	24.551329007953118	0.0
35	6.54039347007116E-4	0.0	0.0	25.0457827542905	0.0
36	6.54039347007116E-4	0.0	0.0	25.427367996172936	0.0
37	6.54039347007116E-4	0.0	0.0	25.853427913651856	0.0
38	6.54039347007116E-4	0.0	0.0	26.359467499850506	0.0
39	6.54039347007116E-4	0.0	0.0	27.883285744184654	0.0
40	6.54039347007116E-4	0.0	0.0	28.30644920169826	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAT	20	6.3112576E-4	46.000004	46
CGGCGAT	20	6.3112576E-4	46.000004	19
GTCGCGC	20	6.3112576E-4	46.000004	10
ACCGGTA	20	6.3112576E-4	46.000004	27
TACCCGT	40	5.6115823E-9	46.000004	12
GCGACTA	40	5.6115823E-9	46.000004	33
CCCTCGC	20	6.3112576E-4	46.000004	37
TCCGCAT	20	6.3112576E-4	46.000004	25
GGTCGCG	20	6.3112576E-4	46.000004	9
CGAGTAC	20	6.3112576E-4	46.000004	12
CCGATAT	20	6.3112576E-4	46.000004	11
CGGCCGT	20	6.3112576E-4	46.000004	21
GCGAAAC	20	6.3112576E-4	46.000004	46
CCGGCTA	20	6.3112576E-4	46.000004	43
GACGTTC	20	6.3112576E-4	46.000004	19
TTTCGCA	20	6.3112576E-4	46.000004	36
CGCATTA	20	6.3112576E-4	46.000004	39
CGGTTCT	20	6.3112576E-4	46.000004	32
CGGTTCG	20	6.3112576E-4	46.000004	30
GACCGTA	20	6.3112576E-4	46.000004	8
>>END_MODULE