FastQCFastQC Report
Sat 14 Jan 2017
SRR2933061.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933061.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912466
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172291.8881799431430872No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC160871.763024594889015No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG75030.8222772136167265No Hit
GGGGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT23910.2620371608366777Illumina PCR Primer Index 8 (95% over 21bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCAGGGAAGCTCG18320.20077460420443063No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA13770.1509097325270202No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG13110.14367658630568153No Hit
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCTT13050.1430190275582871Illumina PCR Primer Index 8 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCG12940.141813503188064No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCAGGGAAGCT11810.12942948011213568No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAG351.0192525E-746.0000041
GCACGAT253.4165983E-546.041
ACTAGCG206.3107663E-446.01
ACTACGG405.6097633E-946.02
TCGACGT206.3107663E-446.026
AATCGCG206.3107663E-446.01
TACCGGT253.4165983E-546.040
CGTTTTT51400.044.165371
CGTAAGG1150.044.0000042
TACGGGA1950.043.6410264
CGTTAGG900.043.4444472
GCAAGGC18350.042.86648646
TAAGTAG1100.041.818181
CTACGGG1100.041.818183
ACGGGAC1600.041.68755
GCGGGAT5850.041.282055
CGATAGG1450.041.241382
ATAGGGA7300.040.9589044
AAGGGAT14100.040.780145
TAGACGA850.040.58823418
Produced by FastQC (version 0.11.5)