##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933061.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	912466
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.500200555417955	33.0	31.0	34.0	31.0	34.0
2	32.8761641529657	34.0	31.0	34.0	31.0	34.0
3	32.88846378933571	34.0	31.0	34.0	31.0	34.0
4	36.3436971898131	37.0	37.0	37.0	35.0	37.0
5	36.20956506872585	37.0	37.0	37.0	35.0	37.0
6	36.30465244732407	37.0	37.0	37.0	35.0	37.0
7	36.5411752328306	37.0	37.0	37.0	35.0	37.0
8	36.50104223061462	37.0	37.0	37.0	35.0	37.0
9	38.30935289643669	39.0	39.0	39.0	37.0	39.0
10	38.01686309407693	39.0	39.0	39.0	35.0	39.0
11	37.72629445919081	39.0	38.0	39.0	35.0	39.0
12	37.360497815809026	39.0	37.0	39.0	35.0	39.0
13	37.28307027330333	39.0	37.0	39.0	35.0	39.0
14	38.01664719562154	41.0	37.0	41.0	33.0	41.0
15	38.326408874412856	41.0	37.0	41.0	33.0	41.0
16	38.556476624882464	41.0	37.0	41.0	34.0	41.0
17	38.6131614767016	41.0	37.0	41.0	35.0	41.0
18	38.52539382289313	40.0	37.0	41.0	35.0	41.0
19	38.44270581040828	40.0	37.0	41.0	35.0	41.0
20	38.25008055094656	40.0	35.0	41.0	34.0	41.0
21	38.213076432436935	40.0	35.0	41.0	34.0	41.0
22	38.17153954229527	40.0	35.0	41.0	34.0	41.0
23	38.17468157717657	40.0	35.0	41.0	34.0	41.0
24	38.154698366843256	40.0	35.0	41.0	34.0	41.0
25	38.013071171966956	40.0	35.0	41.0	34.0	41.0
26	37.93596802510998	40.0	35.0	41.0	34.0	41.0
27	37.84261112194865	40.0	35.0	41.0	34.0	41.0
28	37.818317614026164	40.0	35.0	41.0	34.0	41.0
29	37.76882316710979	40.0	35.0	41.0	34.0	41.0
30	37.70850749507379	40.0	35.0	41.0	34.0	41.0
31	37.556978561392974	40.0	35.0	41.0	34.0	41.0
32	37.409005924604315	40.0	35.0	41.0	33.0	41.0
33	37.25907485867967	40.0	35.0	41.0	33.0	41.0
34	37.05219701336817	40.0	35.0	41.0	33.0	41.0
35	36.92911187923714	40.0	35.0	41.0	32.0	41.0
36	36.84183081890175	40.0	35.0	41.0	32.0	41.0
37	36.7487829683517	40.0	35.0	41.0	32.0	41.0
38	36.71028838334798	40.0	35.0	41.0	32.0	41.0
39	36.633018654941665	40.0	35.0	41.0	31.0	41.0
40	36.56316290141222	40.0	35.0	41.0	31.0	41.0
41	36.42884008828822	40.0	35.0	41.0	31.0	41.0
42	36.46675602159423	39.0	35.0	41.0	31.0	41.0
43	36.44614484265715	39.0	35.0	41.0	31.0	41.0
44	36.42498131437226	39.0	35.0	41.0	31.0	41.0
45	36.352589575940364	39.0	35.0	41.0	31.0	41.0
46	36.30216468339642	39.0	35.0	41.0	31.0	41.0
47	36.22561936554348	39.0	35.0	41.0	31.0	41.0
48	36.118226870919024	39.0	35.0	41.0	31.0	41.0
49	36.094606264781376	39.0	35.0	41.0	31.0	41.0
50	35.99343427590727	38.0	35.0	41.0	30.0	41.0
51	35.92221189611448	38.0	35.0	41.0	30.0	41.0
52	35.68762890891277	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	6.0
14	2.0
15	16.0
16	44.0
17	98.0
18	199.0
19	374.0
20	588.0
21	962.0
22	1660.0
23	2652.0
24	4629.0
25	7575.0
26	10035.0
27	10927.0
28	11216.0
29	11457.0
30	12525.0
31	14791.0
32	18164.0
33	25444.0
34	67012.0
35	89554.0
36	50696.0
37	60663.0
38	101540.0
39	406480.0
40	3151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.166572781889954	21.970681647316173	25.523471559488243	14.339274011305628
2	39.565967389469854	22.35075060331015	25.065043519429764	13.01823848779023
3	33.47291844298856	22.798767296534887	30.622401273033734	13.105912987442819
4	29.38630042105678	26.3226246238216	30.360912077819886	13.93016287730173
5	25.768083413518973	30.399708043916156	29.779301365749518	14.052907176815355
6	27.550396398331557	36.9944743146594	26.87190536414508	8.583223922863976
7	86.3921505020461	1.6975974995232699	10.160707357863197	1.7495446405674293
8	85.10958216525329	1.9796902021554776	10.321370878476568	2.589356754114674
9	79.19155343870347	4.391177315099959	12.277827338224109	4.139441907972461
10	44.48308210936079	28.905296197337766	16.82912020831461	9.782501484986836
11	30.08977868764425	23.15165715763656	31.30713911532046	15.451425039398728
12	30.31948587673404	17.756826007763575	34.17245135709166	17.751236758410723
13	31.332235940846015	17.49917257190953	35.524392141734594	15.64419934550986
14	19.209921246380688	20.203273327444528	40.939059647153975	19.647745779020806
15	15.748532000096441	27.056788965287478	39.70328757455072	17.491391460065362
16	30.266990770067046	23.93908375764138	30.69155453463471	15.102370937656856
17	27.890683050108166	21.553898994592675	28.779702476585427	21.775715478713728
18	23.111655667170066	22.001806094692846	36.225020987083354	18.661517251053738
19	23.091380939125404	23.64888116379131	31.03775921513788	22.22197868194541
20	18.500634544191236	34.650825345821104	29.281091021473678	17.567449088513985
21	23.658744545002225	24.495488051061628	36.4043153388729	15.441452065063247
22	20.149463103282752	20.93886237952976	39.5645426788505	19.34713183833699
23	20.299057718314984	21.89856937135192	38.27627549958026	19.526097410752836
24	21.672697941621934	26.453807593926786	32.0497421273779	19.82375233707338
25	23.69052655112629	24.770128421223365	32.54532223666416	18.994022790986183
26	18.788645275549992	28.004221527158276	28.5117472870222	24.695385910269533
27	21.888705990141002	24.08977430391927	34.018144237703105	20.003375468236626
28	17.45818474332194	27.431816637551425	30.08462781078966	25.025370808336966
29	21.583708324474557	28.782880677197838	29.046123362404735	20.587287635922873
30	25.966775748356653	23.313197423246454	29.427617029018066	21.292409799378824
31	23.723733267869708	24.256246260134624	32.59288565272569	19.427134819269977
32	26.636718518827003	24.235204380218004	27.661743012890344	21.466334088064652
33	26.463670975137703	24.192463061637365	29.8919630978031	19.451902865421836
34	20.612165275199295	24.743935664452156	34.07863964246339	20.56525941788516
35	23.343006753128336	29.241308717256313	28.073374788759253	19.3423097408561
36	26.238676290404246	25.897512893631102	26.275609173382897	21.58820164258175
37	21.95796884486655	29.177415925634488	27.167697207348002	21.696918022150964
38	25.30198385474089	25.805235482746752	29.453919378913845	19.438861283598513
39	21.40211251706913	27.87676472328832	26.212702719882163	24.508420039760388
40	26.41490203470595	25.617283274116513	28.43930623168425	19.528508459493285
41	21.579543785741055	25.409275523690745	26.476164591338197	26.535016099229995
42	20.173025625064387	26.506083514344642	31.10143282051057	22.219458040080397
43	20.103653177214273	26.388818871059307	31.302426610964133	22.205101340762287
44	24.920271001878426	23.90850727588754	28.048606742607397	23.122614979626636
45	20.882641106627535	23.874204627898465	27.333511604815957	27.90964266065804
46	23.124806842117955	24.385566147122194	28.958010490253884	23.531616520505967
47	19.882932624338878	24.23630031146366	32.19648732117142	23.68427974302604
48	20.097406369114026	29.18607378247518	29.266515135906435	21.450004712504356
49	24.15388628179023	25.17639013398855	29.283502070214123	21.386221514007097
50	21.900432454469538	25.336724875228228	28.633505248414735	24.129337421887502
51	21.43378493006863	25.936089673478246	27.168573952344527	25.461551444108604
52	21.770455008734572	26.30519931701565	28.371029715079793	23.553315959169986
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	509.0
1	1594.5
2	2680.0
3	12301.0
4	21922.0
5	12880.5
6	3839.0
7	3931.5
8	4024.0
9	4568.5
10	5113.0
11	5595.0
12	6077.0
13	6085.0
14	5960.0
15	5827.0
16	5595.5
17	5364.0
18	5072.0
19	4780.0
20	4709.5
21	4639.0
22	4845.0
23	5051.0
24	5538.5
25	6026.0
26	6971.5
27	7917.0
28	9463.0
29	11009.0
30	11957.0
31	12905.0
32	14646.5
33	16388.0
34	18331.0
35	20274.0
36	21983.5
37	23693.0
38	25553.0
39	29065.5
40	30718.0
41	32541.5
42	34365.0
43	36609.0
44	38853.0
45	42502.0
46	46151.0
47	52301.0
48	58451.0
49	71181.5
50	83912.0
51	82263.0
52	80614.0
53	80470.0
54	80326.0
55	71652.5
56	62979.0
57	57905.0
58	52831.0
59	53848.0
60	54865.0
61	40890.0
62	26915.0
63	23371.0
64	16899.0
65	13971.0
66	11807.0
67	9643.0
68	7539.5
69	5436.0
70	4965.5
71	4495.0
72	3573.5
73	2652.0
74	2130.0
75	1608.0
76	1270.0
77	932.0
78	787.5
79	643.0
80	482.0
81	321.0
82	257.5
83	194.0
84	159.0
85	124.0
86	83.5
87	43.0
88	31.5
89	22.5
90	25.0
91	15.5
92	6.0
93	4.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	912466.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.054636424080662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.86364865011109	21.598358322869636
2	10.4686053023148	6.292602524565486
3	3.834152061987454	3.457021386530163
4	1.9806771369692746	2.3811412490040222
5	1.367380623167835	2.0548063741321063
6	0.9886804530544807	1.7828658933688661
7	0.8151420897020052	1.7149159397971379
8	0.6631177284802611	1.5943809788669188
9	0.5788080117442852	1.565627791708755
>10	7.217621602179344	45.4965485933978
>50	0.18373559550339727	3.305233688153073
>100	0.028548553269448232	1.7770839440301007
>500	0.006222120584366729	1.313205083493099
>1k	0.0025620496523863006	1.176032463362768
>5k	3.6600709319804293E-4	0.825345764906075
>10k+	7.320141863960859E-4	3.664830001814047
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17229	1.8881799431430872	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	16087	1.763024594889015	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	7503	0.8222772136167265	No Hit
GGGGCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCT	2391	0.2620371608366777	Illumina PCR Primer Index 8 (95% over 21bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCAGGGAAGCTCG	1832	0.20077460420443063	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	1377	0.1509097325270202	No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1311	0.14367658630568153	No Hit
CTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCGTCTTCTGCTT	1305	0.1430190275582871	Illumina PCR Primer Index 8 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGGAAGCTCGTATGCCG	1294	0.141813503188064	No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCAGGGAAGCT	1181	0.12942948011213568	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1477315319146138	0.0
2	0.0	0.0	0.0	0.4061521196406222	0.0
3	0.0	0.0	0.0	0.6618328792524872	0.0
4	0.0	0.0	0.0	0.951816286853428	0.0
5	0.0	0.0	0.0	2.0076364489197407	0.0
6	0.0	0.0	0.0	2.7724868652640207	0.0
7	0.0	0.0	0.0	3.2672998226783245	0.0
8	0.0	0.0	0.0	4.296488855475163	0.0
9	0.0	0.0	0.0	4.834591097092932	0.0
10	0.0	0.0	0.0	5.711884059241659	0.0
11	0.0	0.0	0.0	7.111826632444387	0.0
12	0.0	0.0	0.0	8.047642323111218	0.0
13	0.0	0.0	0.0	8.50826222566101	0.0
14	0.0	0.0	0.0	9.02093886237953	0.0
15	0.0	0.0	0.0	9.40604910210353	0.0
16	0.0	0.0	0.0	9.91741062132726	0.0
17	0.0	0.0	0.0	10.504720175875047	0.0
18	0.0	0.0	0.0	11.22913072925457	0.0
19	0.0	0.0	0.0	11.655995949438116	0.0
20	0.0	0.0	0.0	12.251305802079202	0.0
21	0.0	0.0	0.0	12.769352501901441	0.0
22	0.0	0.0	0.0	13.345483557743522	0.0
23	0.0	0.0	0.0	13.881941902492805	0.0
24	0.0	0.0	0.0	14.294012050859978	0.0
25	0.0	0.0	0.0	14.742795895956672	0.0
26	0.0	0.0	0.0	15.136673585645932	0.0
27	0.0	0.0	0.0	15.74984711759123	0.0
28	0.0	0.0	0.0	16.220220808227374	0.0
29	0.0	0.0	0.0	16.779364929761766	0.0
30	0.0	0.0	0.0	18.071906240890073	0.0
31	0.0	0.0	0.0	18.607159061269133	0.0
32	0.0	0.0	0.0	19.065258321953912	0.0
33	0.0	0.0	0.0	19.554482030015365	0.0
34	0.0	0.0	0.0	20.08962525726986	0.0
35	0.0	0.0	0.0	20.585205366556124	0.0
36	0.0	0.0	0.0	20.965383915674668	0.0
37	0.0	0.0	0.0	21.483978581119736	0.0
38	0.0	0.0	0.0	22.017916284004006	0.0
39	0.0	0.0	0.0	23.604495948342183	0.0
40	0.0	0.0	0.0	24.147310694316282	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAG	35	1.0192525E-7	46.000004	1
GCACGAT	25	3.4165983E-5	46.0	41
ACTAGCG	20	6.3107663E-4	46.0	1
ACTACGG	40	5.6097633E-9	46.0	2
TCGACGT	20	6.3107663E-4	46.0	26
AATCGCG	20	6.3107663E-4	46.0	1
TACCGGT	25	3.4165983E-5	46.0	40
CGTTTTT	5140	0.0	44.16537	1
CGTAAGG	115	0.0	44.000004	2
TACGGGA	195	0.0	43.641026	4
CGTTAGG	90	0.0	43.444447	2
GCAAGGC	1835	0.0	42.866486	46
TAAGTAG	110	0.0	41.81818	1
CTACGGG	110	0.0	41.81818	3
ACGGGAC	160	0.0	41.6875	5
GCGGGAT	585	0.0	41.28205	5
CGATAGG	145	0.0	41.24138	2
ATAGGGA	730	0.0	40.958904	4
AAGGGAT	1410	0.0	40.78014	5
TAGACGA	85	0.0	40.588234	18
>>END_MODULE