FastQCFastQC Report
Sat 14 Jan 2017
SRR2933060.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933060.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences955324
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC191842.0081145244964014No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119141.2471161616373083No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG89000.9316211044629884No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCTT24990.2615866449497762TruSeq Adapter, Index 27 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT23870.24986287374754532Illumina Single End Adapter 1 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA22160.231963187358425No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT20910.2188786212845066Illumina Single End Adapter 1 (95% over 21bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCGGAGTTACTCG19130.20024619919524686No Hit
GGGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT17640.18464939643513614No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG15330.16046911833053498No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCGGAGTTACT13780.1442442563988762No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG13460.1408946074839531No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA13430.14058057789817904No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCG10890.1139927396359769No Hit
CGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC10720.112213238649924TruSeq Adapter, Index 27 (95% over 22bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10400.1088635897350009No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGTCTCTTATACACATCTG9920.10383911636261624No Hit
GGGGTTGGGGATTTAGCTCAGTGGCTGTCTCTTATACACATCTGACGCGGAG9800.10258299801952009No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9730.10185026231938066No Hit
GGGGTTGGGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCG9640.10090817356205853No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCCG206.3109177E-446.044
CTTCGAT206.3109177E-446.031
AGCGTCG206.3109177E-446.036
TACCCGG206.3109177E-446.040
CGAACGT253.4167206E-546.038
CATATCC206.3109177E-446.018
CTCGTGA206.3109177E-446.013
CGTATCT253.4167206E-546.034
ATTAGCG253.4167206E-546.01
GTCGACA253.4167206E-546.021
ACGTTCG253.4167206E-546.041
CCGATAA405.6097633E-946.012
TAGACGT206.3109177E-446.019
TTAGACG253.4167206E-546.018
ATGTGAG1350.046.01
CGTTGCC206.3109177E-446.041
CGCATAT206.3109177E-446.036
CGCATAC206.3109177E-446.012
GAATCGC301.8610699E-646.09
ACCGAAT206.3109177E-446.043
Produced by FastQC (version 0.11.5)