##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933060.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	955324
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43102968207645	33.0	31.0	34.0	31.0	34.0
2	32.80736273766806	34.0	31.0	34.0	31.0	34.0
3	32.86408380821585	34.0	31.0	34.0	31.0	34.0
4	36.30877377727347	37.0	37.0	37.0	35.0	37.0
5	36.15541428876486	37.0	35.0	37.0	35.0	37.0
6	36.25480255913177	37.0	37.0	37.0	35.0	37.0
7	36.48630935682554	37.0	37.0	37.0	35.0	37.0
8	36.43199479967006	37.0	37.0	37.0	35.0	37.0
9	38.264289392918	39.0	39.0	39.0	37.0	39.0
10	37.98139584057346	39.0	38.0	39.0	35.0	39.0
11	37.62164773417186	39.0	37.0	39.0	35.0	39.0
12	37.104382387546	39.0	35.0	39.0	35.0	39.0
13	36.94526568996487	39.0	35.0	39.0	33.0	39.0
14	37.51908671822335	40.0	35.0	41.0	31.0	41.0
15	37.91469386302448	40.0	35.0	41.0	33.0	41.0
16	38.218218112389096	40.0	35.0	41.0	33.0	41.0
17	38.295367854256774	40.0	35.0	41.0	34.0	41.0
18	38.26982782804577	40.0	36.0	41.0	35.0	41.0
19	38.206947590555664	40.0	35.0	41.0	35.0	41.0
20	37.99149817234781	40.0	35.0	41.0	33.0	41.0
21	37.95202360665073	40.0	35.0	41.0	34.0	41.0
22	37.933533544640355	40.0	35.0	41.0	34.0	41.0
23	37.923856199572086	40.0	35.0	41.0	34.0	41.0
24	37.87399772223874	40.0	35.0	41.0	34.0	41.0
25	37.71338833735989	40.0	35.0	41.0	34.0	41.0
26	37.61474536387655	39.0	35.0	41.0	34.0	41.0
27	37.49546436601614	39.0	35.0	41.0	34.0	41.0
28	37.470714647596	39.0	35.0	41.0	34.0	41.0
29	37.42260426829013	39.0	35.0	41.0	34.0	41.0
30	37.36260577563214	39.0	35.0	41.0	34.0	41.0
31	37.198934602292	39.0	35.0	41.0	33.0	41.0
32	37.08395685652198	39.0	35.0	41.0	33.0	41.0
33	36.965677613040185	39.0	35.0	41.0	33.0	41.0
34	36.773215160510986	39.0	35.0	41.0	32.0	41.0
35	36.72582390895654	39.0	35.0	41.0	32.0	41.0
36	36.62388781188372	39.0	35.0	41.0	32.0	41.0
37	36.50851020177448	39.0	35.0	41.0	32.0	41.0
38	36.478935942151566	39.0	35.0	41.0	32.0	41.0
39	36.42190188878328	39.0	35.0	41.0	32.0	41.0
40	36.33686267695567	38.0	35.0	41.0	31.0	41.0
41	36.15748897756154	38.0	35.0	41.0	31.0	41.0
42	36.24887472731764	38.0	35.0	41.0	31.0	41.0
43	36.2080299458613	38.0	35.0	41.0	31.0	41.0
44	36.16814923523328	38.0	35.0	41.0	31.0	41.0
45	36.10122743697426	38.0	35.0	41.0	31.0	41.0
46	36.031637434001446	38.0	35.0	41.0	31.0	41.0
47	35.927841235015556	37.0	35.0	41.0	31.0	41.0
48	35.806401807135586	37.0	35.0	40.0	31.0	41.0
49	35.764222399939705	37.0	35.0	40.0	31.0	41.0
50	35.63272669795797	37.0	35.0	40.0	30.0	41.0
51	35.526169132147835	36.0	35.0	40.0	30.0	41.0
52	35.20936457160084	36.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	1.0
13	4.0
14	13.0
15	24.0
16	65.0
17	132.0
18	251.0
19	416.0
20	728.0
21	1144.0
22	1919.0
23	3056.0
24	4660.0
25	6948.0
26	8426.0
27	9890.0
28	10630.0
29	11982.0
30	14103.0
31	16992.0
32	21225.0
33	30231.0
34	84426.0
35	119488.0
36	58426.0
37	70648.0
38	114072.0
39	363868.0
40	1552.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.97557268528792	22.34163487989415	25.297595370785196	13.385197064032726
2	36.915119896495845	23.662129288073995	25.715359396393268	13.707391419036894
3	34.13260841348066	21.887338745807703	30.45772952422424	13.522323316487391
4	30.92176057546968	26.835921634963633	28.59699955198446	13.645318237582224
5	24.849684504942825	31.095314259874137	29.074638551946773	14.980362683236265
6	27.53568422859679	37.14854855525455	25.74833250289954	9.567434713249117
7	85.8101544606856	1.8965293450180252	9.514573066310488	2.7787431279858983
8	83.6180185989256	2.127864473204902	9.92281152781674	4.331305400052757
9	78.00725198990081	3.7929540135074595	12.832400316541822	5.36739368004991
10	53.59145169596912	15.061801022480331	18.211727120851144	13.135020160699407
11	35.003935837475034	24.663046254464454	24.979483400396095	15.353534507664415
12	34.58386892823796	17.74193886053318	30.448832019293974	17.225360191934882
13	29.967634017359558	21.214373343493936	33.46613295593956	15.351859683206953
14	17.824109935477388	23.000887656962455	39.76431032822372	19.410692079336435
15	13.90847503046087	28.318455309402886	39.99711092781088	17.775958732325368
16	28.830114181157384	24.135685903421248	31.36391423223953	15.670285683181831
17	26.110931997939968	24.02305395865696	26.466204135978998	23.39980990742408
18	23.362231033659782	23.100853741767192	34.629089188589425	18.907826035983604
19	23.874413288057244	25.59602815379913	27.46952866252706	23.060029895616566
20	18.083603049855338	36.36870841724902	27.164919964326238	18.382768568569407
21	23.03480285222605	27.59398905502217	33.29226524194932	16.07894285080245
22	20.611959921450733	22.606152467644485	35.73321721217095	21.048670398733833
23	20.189590128584648	24.599402925080916	33.77712692238445	21.43388002394999
24	22.635880601764427	27.86625270588826	29.41054553219641	20.0873211601509
25	24.761860897454685	24.183732430044678	31.132369750995476	19.922036921505164
26	18.832144905812058	29.616025557821217	26.78954993279767	24.762279603569052
27	23.537773572107472	26.00248711431933	31.414787025134927	19.044952288438267
28	18.41239202616076	28.898258601270356	26.94447119511286	25.744878177456027
29	24.249992672642996	28.53408895830106	25.630048025591318	21.585870343464624
30	28.337192408020734	23.627167327524486	25.687620116316555	22.34802014813822
31	25.760474980216134	25.500877189309595	28.899200690027676	19.83944714044659
32	30.811326837805815	24.037394643073974	23.598590635219047	21.55268788390117
33	29.242644380335886	23.383480368963827	27.814961206878504	19.558914043821783
34	20.41202775184126	23.801139718043302	35.03649023786694	20.750342292248494
35	24.109726124330592	28.775473033232707	27.059196670448976	20.055604171987724
36	28.113184636835253	24.64242497833196	25.59110835695534	21.653282027877452
37	20.489383706470264	30.143176555807244	28.26810589915045	21.099333838572047
38	23.91670260560815	25.999870201104546	30.066029954235425	20.01739723905188
39	20.439662355389377	26.42077452257035	28.118837169379184	25.02072595266109
40	27.489312526430826	23.083686791078208	29.945965975941146	19.481034706549817
41	19.6325016434215	25.26022585007809	26.67252157383254	28.434750932667868
42	19.983168014202512	25.685945291859095	30.7292604393902	23.601626254548194
43	20.239939538837085	26.192579690241217	31.66485925193966	21.90262151898204
44	25.108758913206408	24.24444481662766	27.61858803924114	23.028208230924797
45	20.611645891864956	24.233872487239932	26.63735026022585	28.51713136066926
46	24.16614677324133	23.33491045969744	28.421247660479587	24.077695106581643
47	19.391012891961264	24.16154100598331	31.911477153300872	24.535968948754558
48	19.534943118774365	28.99822468607509	28.411931449434956	23.05490074571559
49	25.35213184218129	23.86007260364023	29.670876058803085	21.11691949537539
50	22.128304114624985	24.29762049315206	29.292261054888186	24.28181433733477
51	20.683244637421442	25.412320846121318	26.958393173415512	26.94604134304173
52	20.72302171828615	27.17884194262889	28.97624261507091	23.12189372401405
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	904.0
1	2253.5
2	3603.0
3	8989.5
4	14376.0
5	8451.0
6	2526.0
7	2975.5
8	3425.0
9	4408.0
10	5391.0
11	6468.5
12	7546.0
13	7832.0
14	7785.5
15	7453.0
16	6709.0
17	5965.0
18	5410.5
19	4856.0
20	4558.5
21	4261.0
22	4062.0
23	3863.0
24	4263.5
25	4664.0
26	4901.5
27	5139.0
28	6327.0
29	7515.0
30	8231.5
31	8948.0
32	10547.5
33	12147.0
34	13806.0
35	15465.0
36	16844.0
37	18223.0
38	19975.5
39	24286.0
40	26844.0
41	31378.5
42	35913.0
43	40601.0
44	45289.0
45	53957.5
46	62626.0
47	76188.0
48	89750.0
49	96183.0
50	102616.0
51	94014.0
52	85412.0
53	77192.0
54	68972.0
55	63715.5
56	58459.0
57	56537.5
58	54616.0
59	58292.5
60	61969.0
61	45138.0
62	28307.0
63	23570.5
64	16824.0
65	14814.0
66	12817.5
67	10821.0
68	9617.0
69	8413.0
70	6866.0
71	5319.0
72	4577.0
73	3835.0
74	3222.5
75	2610.0
76	2276.5
77	1943.0
78	1442.5
79	942.0
80	660.5
81	379.0
82	337.0
83	295.0
84	219.5
85	144.0
86	101.0
87	58.0
88	35.5
89	10.5
90	8.0
91	6.0
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	955324.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.658001078056333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.50733701417434	14.565406440496947
2	11.728476810929791	4.845737732082927
3	5.072933537358501	3.1438999945098014
4	2.6030045927928147	2.150914867363982
5	1.5574486304929738	1.6086887743870606
6	0.9926945921522197	1.2304251572916747
7	0.7100014478696005	1.0267047472858235
8	0.5144187957245399	0.8501491229319988
9	0.4410386611176451	0.8199879423149552
>10	3.7284544298339024	16.410171006003726
>50	1.158046493634164	17.375692680485873
>100	0.9708874643591594	28.349770136205816
>500	0.007120180461512956	1.14329941861579
>1k	0.006611596142833459	2.276827945306266
>5k	5.085843186794968E-4	0.9350638509171597
>10k+	0.0010171686373589936	3.267260183800205
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	19184	2.0081145244964014	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11914	1.2471161616373083	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	8900	0.9316211044629884	No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCTT	2499	0.2615866449497762	TruSeq Adapter, Index 27 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	2387	0.24986287374754532	Illumina Single End Adapter 1 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	2216	0.231963187358425	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	2091	0.2188786212845066	Illumina Single End Adapter 1 (95% over 21bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCGGAGTTACTCG	1913	0.20024619919524686	No Hit
GGGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCT	1764	0.18464939643513614	No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1533	0.16046911833053498	No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCGGAGTTACT	1378	0.1442442563988762	No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1346	0.1408946074839531	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1343	0.14058057789817904	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCG	1089	0.1139927396359769	No Hit
CGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	1072	0.112213238649924	TruSeq Adapter, Index 27 (95% over 22bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1040	0.1088635897350009	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGTCTCTTATACACATCTG	992	0.10383911636261624	No Hit
GGGGTTGGGGATTTAGCTCAGTGGCTGTCTCTTATACACATCTGACGCGGAG	980	0.10258299801952009	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	973	0.10185026231938066	No Hit
GGGGTTGGGGCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCG	964	0.10090817356205853	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2715309151659542	0.0
2	0.0	0.0	0.0	0.9137214180738681	0.0
3	0.0	0.0	0.0	1.38246291310592	0.0
4	0.0	0.0	0.0	1.921233005765583	0.0
5	0.0	0.0	0.0	3.5428817867027314	0.0
6	0.0	0.0	0.0	4.963342279687311	0.0
7	0.0	0.0	0.0	6.153200380185152	0.0
8	0.0	0.0	0.0	8.265363374101351	0.0
9	0.0	0.0	0.0	9.382889993342573	0.0
10	0.0	0.0	0.0	10.533913101733024	0.0
11	0.0	0.0	0.0	12.361879320523718	0.0
12	0.0	0.0	0.0	13.357457783956018	0.0
13	0.0	0.0	0.0	13.80369382534093	0.0
14	0.0	0.0	0.0	14.021630357868116	0.0
15	0.0	0.0	0.0	14.315248020566845	0.0
16	0.0	0.0	0.0	14.866265267071695	0.0
17	0.0	0.0	0.0	15.447848059925219	0.0
18	0.0	0.0	0.0	16.14666856480105	0.0
19	0.0	0.0	0.0	16.540252312304517	0.0
20	0.0	0.0	0.0	17.08153464165037	0.0
21	1.0467652859134702E-4	0.0	0.0	17.59507769091952	0.0
22	1.0467652859134702E-4	0.0	0.0	18.101398059715866	0.0
23	1.0467652859134702E-4	0.0	0.0	18.55307728058753	0.0
24	3.1402958577404105E-4	0.0	0.0	18.88343640482182	0.0
25	3.1402958577404105E-4	0.0	0.0	19.293454367314126	0.0
26	3.1402958577404105E-4	0.0	0.0	19.6300940832639	0.0
27	3.1402958577404105E-4	0.0	0.0	20.16404905560836	0.0
28	3.1402958577404105E-4	0.0	0.0	20.53418526070736	0.0
29	3.1402958577404105E-4	0.0	0.0	21.030142653173165	0.0
30	3.1402958577404105E-4	0.0	0.0	22.28573761362637	0.0
31	3.1402958577404105E-4	0.0	0.0	22.74160389564169	0.0
32	3.1402958577404105E-4	0.0	0.0	23.046317270371098	0.0
33	3.1402958577404105E-4	0.0	0.0	23.456439909391996	0.0
34	4.1870611436538806E-4	0.0	0.0	23.92894975945334	0.0
35	4.1870611436538806E-4	0.0	0.0	24.34639975547563	0.0
36	4.1870611436538806E-4	0.0	0.0	24.613324903383564	0.0
37	4.1870611436538806E-4	0.0	0.0	25.00701332741562	0.0
38	4.1870611436538806E-4	0.0	0.0	25.472614526589933	0.0
39	4.1870611436538806E-4	0.0	0.0	27.179365325271846	0.0
40	4.1870611436538806E-4	0.0	0.0	27.532125226624686	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	20	6.3109177E-4	46.0	44
CTTCGAT	20	6.3109177E-4	46.0	31
AGCGTCG	20	6.3109177E-4	46.0	36
TACCCGG	20	6.3109177E-4	46.0	40
CGAACGT	25	3.4167206E-5	46.0	38
CATATCC	20	6.3109177E-4	46.0	18
CTCGTGA	20	6.3109177E-4	46.0	13
CGTATCT	25	3.4167206E-5	46.0	34
ATTAGCG	25	3.4167206E-5	46.0	1
GTCGACA	25	3.4167206E-5	46.0	21
ACGTTCG	25	3.4167206E-5	46.0	41
CCGATAA	40	5.6097633E-9	46.0	12
TAGACGT	20	6.3109177E-4	46.0	19
TTAGACG	25	3.4167206E-5	46.0	18
ATGTGAG	135	0.0	46.0	1
CGTTGCC	20	6.3109177E-4	46.0	41
CGCATAT	20	6.3109177E-4	46.0	36
CGCATAC	20	6.3109177E-4	46.0	12
GAATCGC	30	1.8610699E-6	46.0	9
ACCGAAT	20	6.3109177E-4	46.0	43
>>END_MODULE