##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933058.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1792384
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48045619688638	33.0	31.0	34.0	31.0	34.0
2	32.865767603370706	34.0	31.0	34.0	31.0	34.0
3	32.89773898896665	34.0	31.0	34.0	31.0	34.0
4	36.36861130204242	37.0	37.0	37.0	35.0	37.0
5	36.299657327894025	37.0	37.0	37.0	35.0	37.0
6	36.38515965328858	37.0	37.0	37.0	35.0	37.0
7	36.51895966489324	37.0	37.0	37.0	35.0	37.0
8	36.45252914554024	37.0	37.0	37.0	35.0	37.0
9	38.261106437013495	39.0	39.0	39.0	37.0	39.0
10	37.98726277404842	39.0	38.0	39.0	35.0	39.0
11	37.67962947671928	39.0	37.0	39.0	35.0	39.0
12	37.26352109815754	39.0	35.0	39.0	35.0	39.0
13	37.188173962722274	39.0	35.0	39.0	34.0	39.0
14	38.242109949653646	40.0	37.0	41.0	33.0	41.0
15	38.38448122723702	40.0	36.0	41.0	33.0	41.0
16	38.5028353299293	41.0	37.0	41.0	35.0	41.0
17	38.50296197689781	40.0	36.0	41.0	35.0	41.0
18	38.381899191244734	40.0	36.0	41.0	35.0	41.0
19	38.29592263711348	40.0	36.0	41.0	35.0	41.0
20	38.117068663857744	40.0	35.0	41.0	34.0	41.0
21	38.06900362868671	40.0	35.0	41.0	34.0	41.0
22	38.004965453831325	40.0	35.0	41.0	34.0	41.0
23	37.986204407091336	40.0	35.0	41.0	34.0	41.0
24	37.94140876062272	40.0	35.0	41.0	34.0	41.0
25	37.8396543374634	40.0	35.0	41.0	34.0	41.0
26	37.72713771156181	40.0	35.0	41.0	34.0	41.0
27	37.640654569556524	40.0	35.0	41.0	34.0	41.0
28	37.599232642112405	40.0	35.0	41.0	34.0	41.0
29	37.54109777815468	40.0	35.0	41.0	34.0	41.0
30	37.469765407412694	40.0	35.0	41.0	34.0	41.0
31	37.29988774726844	40.0	35.0	41.0	33.0	41.0
32	37.116093984324785	40.0	35.0	41.0	33.0	41.0
33	36.90742106602157	40.0	35.0	41.0	33.0	41.0
34	36.68628653235021	39.0	35.0	41.0	32.0	41.0
35	36.527929840926944	39.0	35.0	41.0	31.0	41.0
36	36.42887740573448	39.0	35.0	41.0	31.0	41.0
37	36.328883765978716	39.0	35.0	41.0	31.0	41.0
38	36.27809554202671	39.0	35.0	41.0	31.0	41.0
39	36.24572468845962	39.0	35.0	41.0	31.0	41.0
40	36.16638845247447	39.0	35.0	41.0	31.0	41.0
41	36.04422378240377	39.0	35.0	41.0	30.0	41.0
42	36.0573320226023	39.0	35.0	41.0	30.0	41.0
43	36.01845251910305	39.0	35.0	41.0	30.0	41.0
44	35.97154515996572	38.0	35.0	41.0	30.0	41.0
45	35.90961479236592	38.0	35.0	41.0	30.0	41.0
46	35.845505204241945	38.0	35.0	41.0	30.0	41.0
47	35.7582169892166	38.0	35.0	41.0	30.0	41.0
48	35.66845664768264	38.0	35.0	41.0	30.0	41.0
49	35.63156276779976	37.0	35.0	41.0	29.0	41.0
50	35.526070864279085	37.0	35.0	40.0	29.0	41.0
51	35.44674634453332	37.0	35.0	40.0	29.0	41.0
52	35.20527074555452	37.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	3.0
12	6.0
13	3.0
14	19.0
15	47.0
16	119.0
17	221.0
18	494.0
19	880.0
20	1475.0
21	2399.0
22	3860.0
23	5967.0
24	9665.0
25	15854.0
26	21841.0
27	23998.0
28	24152.0
29	24097.0
30	25968.0
31	30469.0
32	37115.0
33	51238.0
34	125600.0
35	207962.0
36	106851.0
37	131614.0
38	216270.0
39	720267.0
40	3926.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.73811861743912	21.794604281225453	24.411900574876814	16.055376526458616
2	39.08241760694137	22.727886435049633	24.720651378276084	13.469044579732914
3	32.05217185603085	23.278159144469043	30.992521691780333	13.677147307719775
4	28.624948671713202	26.52433853459973	30.353038188245375	14.497674605441691
5	26.11449332285939	30.448051310433478	28.48334955009641	14.954105816610728
6	28.640793490680565	36.91608494608298	25.597862957937583	8.845258605298865
7	85.19474621509676	1.820592015996572	10.763541741055487	2.2211200278511747
8	83.49717471256159	2.218218863814897	11.282515353852746	3.0020910697707635
9	76.98495411697493	4.258462472327358	13.849320234949653	4.907263175748054
10	45.87694377990431	24.078155127472684	18.183436138684566	11.861464953938441
11	32.55050257087767	22.66015541312576	29.513764907519818	15.275577108476753
12	32.10807505534528	17.758304024137686	31.670724576876385	18.462896343640647
13	30.886071288295362	18.62597523744912	33.82004079482968	16.667912679425836
14	20.54336570734843	19.403487199171607	37.97300132114547	22.0801457723345
15	17.52648985931586	25.519866278654575	38.27327179890024	18.68037206312933
16	28.83812843676355	23.816659822895094	30.917091426837107	16.428120313504248
17	27.461916642862246	22.854366028708135	29.796684192673002	19.887033135756624
18	23.27470006427194	23.1433108083982	34.31329447261301	19.268694654716846
19	23.415015978718845	25.294133399985718	29.642810915518105	21.648039705777332
20	20.218881668213953	32.59535903020781	28.907365832321645	18.27839346925659
21	25.288554238377493	25.089768710276367	33.15963543526387	16.462041616082267
22	21.874442083839178	22.007337713347138	35.90329973934157	20.214920463472115
23	21.815860886952795	22.97671704277655	35.526315789473685	19.68110628079697
24	22.51331187959723	25.46301462186674	32.22724594729701	19.79642755123902
25	25.197446529315148	24.284584106977075	30.75295249232307	19.765016871384702
26	20.719555630936227	26.538788563165035	28.529377633364277	24.212278172534457
27	22.675777065628793	23.440735913732773	32.64741260801257	21.236074412625864
28	19.09261631436121	27.168731700349923	29.36067271299007	24.377979272298795
29	21.586836302935087	27.959187317003497	28.036402913661355	22.417573466400057
30	25.198897111333284	24.152134810397772	28.425995768763833	22.222972309505106
31	22.28010292437335	24.698781064771836	31.092444476183672	21.928671534671142
32	24.97020727701207	25.497940173534246	27.21950207098479	22.3123504784689
33	24.48069163750625	24.64282207384132	28.983856137970438	21.892630150681995
34	19.748614136256517	25.443989680782693	32.55200894451189	22.255387238448904
35	20.097479111618938	28.461646611440404	28.57607521602514	22.86479906091552
36	23.42762488395344	27.037454027708346	26.18294963579233	23.351971452545882
37	20.254253552810113	29.01364886095837	26.960182639434404	23.771914946797114
38	21.99188343569235	26.02890898378919	29.028879972148825	22.950327608369637
39	21.049841998143254	26.405613975576664	26.17753784903235	26.36700617724773
40	25.110969524387635	24.028723755623798	28.54388345354567	22.316423266442907
41	20.24560585231736	24.789498232521602	27.361101192601584	27.60379472255945
42	19.094680604156252	26.191429961436835	29.937613814896807	24.776275619510105
43	19.685123277154894	25.557971952438763	30.32871304363351	24.428191726772834
44	22.58567360565593	25.02996009783618	28.623107548382485	23.761258748125403
45	20.532095800899807	23.646774351924588	27.974530011426125	27.84659983574948
46	22.319938138256088	24.36732307362708	28.61061022638006	24.70212856173677
47	19.158283046490038	23.959374776833535	31.841279547239875	25.04106262943655
48	20.04018112190245	26.209394861815326	29.826476915660933	23.923947100621294
49	23.02614841462544	24.47232289509391	29.779054041991003	22.722474648289655
50	21.40718729915018	24.676241251874597	29.757351103335	24.15922034564022
51	20.58693895950868	24.986386845675927	28.560397771906022	25.86627642290938
52	20.82583865957295	25.258761515389562	29.106486110119263	24.808913714918233
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1034.0
1	3402.5
2	5771.0
3	24437.0
4	43103.0
5	25860.0
6	8617.0
7	8665.5
8	8714.0
9	9932.0
10	11150.0
11	12069.5
12	12989.0
13	13375.5
14	13403.0
15	13044.0
16	12347.5
17	11651.0
18	10971.5
19	10292.0
20	9864.5
21	9437.0
22	9321.0
23	9205.0
24	9943.0
25	10681.0
26	12078.0
27	13475.0
28	15726.5
29	17978.0
30	19931.5
31	21885.0
32	23989.0
33	26093.0
34	30813.5
35	35534.0
36	37535.5
37	39537.0
38	42715.0
39	50277.5
40	54662.0
41	57756.0
42	60850.0
43	65698.0
44	70546.0
45	84098.5
46	97651.0
47	112555.0
48	127459.0
49	143783.5
50	160108.0
51	158574.0
52	157040.0
53	142049.5
54	127059.0
55	120114.0
56	113169.0
57	105777.0
58	98385.0
59	101328.5
60	104272.0
61	88310.5
62	72349.0
63	62024.5
64	45921.5
65	40143.0
66	34363.5
67	28584.0
68	24489.5
69	20395.0
70	16749.5
71	13104.0
72	10890.5
73	8677.0
74	7933.5
75	7190.0
76	5718.5
77	4247.0
78	3490.0
79	2733.0
80	2014.0
81	1295.0
82	890.0
83	485.0
84	375.0
85	265.0
86	175.5
87	86.0
88	52.5
89	40.0
90	61.0
91	32.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1792384.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.39451704791467
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.36350408867234	16.92909230049288
2	9.955835909259823	4.658239458108399
3	3.948583132670755	2.771255862371228
4	2.1335528769572094	1.996537566104113
5	1.3401050220327129	1.5675554891970183
6	0.9027802088778224	1.2672064192267307
7	0.716698964367796	1.1736778298087633
8	0.5697409190774478	1.066305091540154
9	0.4599989495002988	0.968530793949682
>10	6.126316085259284	34.12919109662222
>50	1.2413327405894639	20.09814378533924
>100	0.23050602384689242	6.941987930079918
>500	0.005522539444061011	0.9559465141299236
>1k	0.004562097801615618	1.7211868104039834
>5k	2.4011041061134828E-4	0.3365866442981844
>10k+	7.203312318340449E-4	3.418556408327588
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33213	1.8530069449403699	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	17128	0.9555988002570878	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	10517	0.5867604263372134	No Hit
GGGGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT	5992	0.33430336356495033	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTC	2379	0.13272825465971577	No Hit
CGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC	2180	0.12162572305934442	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCTT	2163	0.12067726558594585	TruSeq Adapter, Index 13 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT	2104	0.11738556023709204	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	1948	0.10868206812825823	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12631221881025495	0.0
2	5.579161608226809E-5	0.0	0.0	0.31522263086481467	0.0
3	5.579161608226809E-5	0.0	0.0	0.5621005320288509	0.0
4	5.579161608226809E-5	0.0	0.0	0.827222291651789	0.0
5	5.579161608226809E-5	0.0	0.0	1.8423507462686568	0.0
6	5.579161608226809E-5	0.0	0.0	2.904790491323288	0.0
7	5.579161608226809E-5	0.0	0.0	3.6706977969006642	0.0
8	5.579161608226809E-5	0.0	0.0	5.044120009997858	0.0
9	5.579161608226809E-5	0.0	0.0	6.042008855245305	0.0
10	5.579161608226809E-5	0.0	0.0	7.0599268906662855	0.0
11	5.579161608226809E-5	0.0	0.0	8.131516460758409	0.0
12	5.579161608226809E-5	0.0	0.0	8.792647111333286	0.0
13	5.579161608226809E-5	0.0	0.0	9.164386649289439	0.0
14	5.579161608226809E-5	0.0	0.0	9.76436968863815	0.0
15	5.579161608226809E-5	0.0	0.0	10.066648664571877	0.0
16	5.579161608226809E-5	0.0	0.0	10.378914339784332	0.0
17	5.579161608226809E-5	0.0	0.0	10.715728326072984	0.0
18	5.579161608226809E-5	0.0	0.0	11.131152699421552	0.0
19	5.579161608226809E-5	0.0	0.0	11.409218113975577	0.0
20	5.579161608226809E-5	0.0	0.0	11.796132971506106	0.0
21	5.579161608226809E-5	0.0	0.0	12.143491573234307	0.0
22	5.579161608226809E-5	0.0	0.0	12.528118974505464	0.0
23	1.1158323216453618E-4	0.0	0.0	12.877374491180461	0.0
24	1.1158323216453618E-4	0.0	0.0	13.16096327572663	0.0
25	1.1158323216453618E-4	0.0	0.0	13.462516960651289	0.0
26	1.1158323216453618E-4	0.0	0.0	13.724570181389701	0.0
27	1.1158323216453618E-4	0.0	0.0	14.071705616653574	0.0
28	1.1158323216453618E-4	0.0	0.0	14.38079116974934	0.0
29	1.6737484824680426E-4	0.0	0.0	14.742041883882026	0.0
30	1.6737484824680426E-4	0.0	0.0	15.659088677426265	0.0
31	1.6737484824680426E-4	0.0	0.0	16.068654931086197	0.0
32	1.6737484824680426E-4	0.0	0.0	16.439613386417196	0.0
33	1.6737484824680426E-4	0.0	0.0	16.836124401913874	0.0
34	1.6737484824680426E-4	0.0	0.0	17.212271477540526	0.0
35	1.6737484824680426E-4	0.0	0.0	17.577762354495466	0.0
36	1.6737484824680426E-4	0.0	0.0	17.863248053988432	0.0
37	1.6737484824680426E-4	0.0	0.0	18.225224059130188	0.0
38	1.6737484824680426E-4	0.0	0.0	18.610911501106905	0.0
39	1.6737484824680426E-4	0.0	0.0	19.827782439477254	0.0
40	1.6737484824680426E-4	0.0	0.0	20.284883150039278	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	55	1.8189894E-12	46.000004	1
TTACGCG	30	1.8619903E-6	46.0	1
ACGTATA	25	3.4179262E-5	46.0	36
CGTTTTT	9955	0.0	44.428932	1
GGCGATA	80	0.0	43.125004	8
GTACGCG	65	0.0	42.46154	1
CCATACG	175	0.0	42.057144	2
CATACGA	835	0.0	41.868263	18
CGTTAGG	220	0.0	41.818184	2
TAGGGAT	1625	0.0	40.338463	5
GCGAGAC	1015	0.0	40.334976	21
CGACCAA	985	0.0	40.162434	29
ACGACCA	995	0.0	39.527637	28
ACATACG	885	0.0	39.502827	17
GGGCGAT	1095	0.0	39.488586	7
GCAAGGC	2105	0.0	39.44418	46
TACGAAA	895	0.0	39.318436	20
CGAATAT	240	0.0	39.291668	14
ACAACGA	335	0.0	39.134327	13
ACACGAC	1005	0.0	39.134327	26
>>END_MODULE