FastQCFastQC Report
Sat 14 Jan 2017
SRR2933057.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933057.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1068142
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163331.5291038082951518No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC119721.1208247592548557No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG68650.6427048089111747No Hit
GGGGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT23370.218791134512078No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCTT18890.17684914552559491TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT15670.1467033409415602TruSeq Adapter, Index 20 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC13590.12723027462640735TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT13390.1253578644037965TruSeq Adapter, Index 20 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA12410.11618305431300334No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCGTCAATCCTCG11390.10663376217768798No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCG11010.10307618275472735No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA206.3112547E-446.042
TCGTTAG453.092282E-1046.01
CAAGTCG206.3112547E-446.01
ACGTTTA206.3112547E-446.015
ACGTTAG405.6115823E-946.01
GCGAAAT206.3112547E-446.030
GCAACGA453.092282E-1046.011
GTCTAGA253.4169927E-546.09
CGACATA206.3112547E-446.012
TACGCGA206.3112547E-446.015
ACTACGG301.8612791E-646.02
TATAGCG301.8612791E-646.01
CGCAACG206.3112547E-446.017
TTCGCAT453.092282E-1046.041
TAAGCGA206.3112547E-446.038
TGTCGTA253.4169927E-546.031
CGATAGT206.3112547E-446.029
TGCGTAT206.3112547E-446.044
CGTTAGG1400.044.357142
CGTTTTT42500.043.4564741
Produced by FastQC (version 0.11.5)