##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933057.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1068142
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.465913708102484	33.0	31.0	34.0	31.0	34.0
2	32.850165989166236	34.0	31.0	34.0	31.0	34.0
3	32.884239174192196	34.0	31.0	34.0	31.0	34.0
4	36.34067006072226	37.0	37.0	37.0	35.0	37.0
5	36.259436479419406	37.0	37.0	37.0	35.0	37.0
6	36.35121921991645	37.0	37.0	37.0	35.0	37.0
7	36.51563275294858	37.0	37.0	37.0	35.0	37.0
8	36.45600678561465	37.0	37.0	37.0	35.0	37.0
9	38.26920016252521	39.0	39.0	39.0	37.0	39.0
10	37.96631627630034	39.0	38.0	39.0	35.0	39.0
11	37.68745447702646	39.0	37.0	39.0	35.0	39.0
12	37.30560824309877	39.0	37.0	39.0	35.0	39.0
13	37.21692902254569	39.0	37.0	39.0	34.0	39.0
14	38.245905506945704	40.0	37.0	41.0	33.0	41.0
15	38.434140779035	40.0	37.0	41.0	33.0	41.0
16	38.55710851178963	41.0	37.0	41.0	35.0	41.0
17	38.540154773429	40.0	37.0	41.0	35.0	41.0
18	38.480908905370256	40.0	37.0	41.0	35.0	41.0
19	38.41888531674628	40.0	36.0	41.0	35.0	41.0
20	38.28102349687588	40.0	35.0	41.0	34.0	41.0
21	38.22172894615135	40.0	35.0	41.0	34.0	41.0
22	38.15456371905608	40.0	35.0	41.0	34.0	41.0
23	38.122653167837235	40.0	35.0	41.0	34.0	41.0
24	38.057403416399694	40.0	35.0	41.0	34.0	41.0
25	37.931716007796716	40.0	35.0	41.0	34.0	41.0
26	37.81320648378212	40.0	35.0	41.0	34.0	41.0
27	37.71153273628413	40.0	35.0	41.0	34.0	41.0
28	37.6840953730871	40.0	35.0	41.0	34.0	41.0
29	37.62900063849189	40.0	35.0	41.0	34.0	41.0
30	37.54863023830165	40.0	35.0	41.0	34.0	41.0
31	37.42115280552586	40.0	35.0	41.0	33.0	41.0
32	37.300753083391534	40.0	35.0	41.0	33.0	41.0
33	37.13445309706013	40.0	35.0	41.0	33.0	41.0
34	36.952722578084185	40.0	35.0	41.0	33.0	41.0
35	36.861432281475686	39.0	35.0	41.0	32.0	41.0
36	36.74492436398906	39.0	35.0	41.0	32.0	41.0
37	36.64643933110017	39.0	35.0	41.0	32.0	41.0
38	36.60070196659246	39.0	35.0	41.0	32.0	41.0
39	36.56711092719882	39.0	35.0	41.0	32.0	41.0
40	36.52451921186509	39.0	35.0	41.0	31.0	41.0
41	36.3993111402791	39.0	35.0	41.0	31.0	41.0
42	36.408029082275576	39.0	35.0	41.0	31.0	41.0
43	36.36318485744405	39.0	35.0	41.0	31.0	41.0
44	36.31505736128717	39.0	35.0	41.0	31.0	41.0
45	36.235942412151196	38.0	35.0	41.0	31.0	41.0
46	36.17125906480599	38.0	35.0	41.0	31.0	41.0
47	36.06409260191997	38.0	35.0	41.0	31.0	41.0
48	35.96370520024491	38.0	35.0	41.0	31.0	41.0
49	35.92068189435487	38.0	35.0	41.0	31.0	41.0
50	35.8083194931011	37.0	35.0	40.0	31.0	41.0
51	35.71780062950432	37.0	35.0	40.0	30.0	41.0
52	35.4557877136186	37.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	1.0
12	2.0
13	5.0
14	9.0
15	27.0
16	63.0
17	136.0
18	258.0
19	494.0
20	821.0
21	1345.0
22	2141.0
23	3185.0
24	5061.0
25	7754.0
26	10485.0
27	11810.0
28	12425.0
29	13065.0
30	14460.0
31	17241.0
32	21356.0
33	30167.0
34	72636.0
35	119807.0
36	71841.0
37	83083.0
38	129207.0
39	436603.0
40	2652.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.91537080275844	22.90060684815315	25.15452065362096	14.029501695467456
2	37.96424070956858	23.227810534554393	25.311990353342534	13.495958402534495
3	33.79719175914813	23.315813815017105	29.09360365943854	13.793390766396227
4	29.270546425475263	26.745133137728878	29.622746788348365	14.36157364844749
5	25.860232066522993	31.16083816571205	27.779733406232506	15.199196361532454
6	28.07969352389476	37.66989782257415	24.90240061714641	9.348008036384675
7	86.39347577382034	2.070979326718732	9.249893740719866	2.285651158741066
8	84.61112848291707	2.3180438555922342	9.684761014921236	3.3860666465694633
9	78.19559571667438	4.524866543961383	12.340400433650208	4.939137305714034
10	45.907566596950595	24.534378387892247	17.715996562254833	11.842058452902329
11	32.78431144922679	24.56452419247628	27.53922231313814	15.111942045158791
12	31.657494977259574	19.670043870571515	29.80362161585257	18.868839536316333
13	29.146031145671643	22.21399401952175	32.4864109828094	16.15356385199721
14	19.172825335957207	22.938335914138758	37.12493282728326	20.763905922620776
15	18.00050929558055	27.960889095270105	36.410701947868354	17.627899661280992
16	28.23454184930468	25.685910674797917	30.17510780401857	15.904439671878833
17	25.379677982889916	25.208258827009892	28.221903080302056	21.190160109798136
18	22.815505803535487	25.32940376841281	32.183455008790965	19.671635419260735
19	22.879448612637646	27.77215014483093	27.77701841140972	21.571382831121706
20	20.352162914668646	34.89938603668801	26.874235822577898	17.87421522606545
21	24.346482022053248	27.921287619061886	31.5561039637052	16.176126395179665
22	21.272171677548492	24.17824596355166	33.15008678621382	21.39949557268603
23	21.35081290689815	25.730005935540408	32.61317315488015	20.306008002681292
24	22.0378938380852	28.960007190055254	29.622372306303845	19.3797266655557
25	24.18489301984193	26.592531704586094	29.591383917119636	19.63119135845234
26	19.95717797820889	30.03065135534414	26.73183902514834	23.28033164129863
27	23.136156054157595	26.39723931836778	29.976164217866163	20.49044040960846
28	19.11000597298861	29.951916505483357	26.785951680581793	24.152125840946244
29	22.42482741058773	28.63121195496479	26.23162463417785	22.71233600026963
30	25.648743331879093	25.849559328254106	27.488948098661037	21.012749241205757
31	23.08887769603667	25.871560148369788	29.729942273592837	21.309619882000707
32	25.687595843998267	26.25718303371649	25.69087256188784	22.364348560397403
33	23.86021708724121	26.086044739369857	28.703206128024178	21.350532045364755
34	19.400323178004424	27.4958760164847	31.550393112526237	21.553407692984642
35	20.813712034542224	30.347369544498765	27.234393928897095	21.604524492061916
36	23.484611596585474	29.156142160873742	25.17006165846863	22.189184584072155
37	20.298892843835368	29.853614968796283	27.477526396303116	22.369965791065233
38	21.59666036912695	27.872511332762873	28.518024757007964	22.012803541102212
39	20.25554654718193	29.226263923710516	25.85283604614368	24.665353482963877
40	24.5281058136465	26.29846967912506	28.480763793578006	20.692660713650433
41	19.62969343027425	26.468671768360387	26.879665812223468	27.021968989141893
42	19.483083709843825	27.49278653961739	28.99062109719494	24.033508653343844
43	19.94144973233896	27.695755807748405	29.71823970970152	22.644554750211114
44	22.549529931413616	27.27624229737245	27.07111975748543	23.103108013728512
45	20.325761930529836	26.228441536799412	26.411656877081885	27.034139655588863
46	22.824118890559493	25.969674444034595	27.534447667070484	23.671758998335427
47	19.62978705078538	25.506159293427277	30.669798584832353	24.194255070954984
48	19.702998290489468	28.920312093335905	28.285377786848564	23.091311829326063
49	22.956966395853733	26.20569175259469	28.130248599905254	22.707093251646317
50	21.04289504578979	26.662091744356086	28.567175525351495	23.727837684502624
51	20.74050079483814	26.14848962029393	27.435116304760975	25.675893280106955
52	21.349034117186665	26.606855642789068	28.475146562910176	23.56896367711409
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	706.0
1	1927.5
2	3149.0
3	11536.5
4	19924.0
5	11772.5
6	3621.0
7	4130.5
8	4640.0
9	6139.0
10	7638.0
11	8921.5
12	10205.0
13	10566.5
14	10395.0
15	9862.0
16	9015.5
17	8169.0
18	7300.5
19	6432.0
20	6104.0
21	5776.0
22	5579.0
23	5382.0
24	5871.5
25	6361.0
26	7224.5
27	8088.0
28	9340.5
29	10593.0
30	11324.0
31	12055.0
32	14123.5
33	16192.0
34	17877.5
35	19563.0
36	20804.5
37	22046.0
38	23738.5
39	28670.5
40	31910.0
41	34819.0
42	37728.0
43	44445.0
44	51162.0
45	58683.5
46	66205.0
47	78001.0
48	89797.0
49	97516.5
50	105236.0
51	101161.0
52	97086.0
53	85497.0
54	73908.0
55	68941.5
56	63975.0
57	59225.0
58	54475.0
59	56413.5
60	58352.0
61	46681.5
62	35011.0
63	31349.0
64	22944.0
65	18201.0
66	16166.0
67	14131.0
68	11755.0
69	9379.0
70	7446.5
71	5514.0
72	4705.0
73	3896.0
74	3477.5
75	3059.0
76	2688.5
77	2318.0
78	1680.5
79	1043.0
80	812.5
81	582.0
82	485.5
83	389.0
84	278.5
85	168.0
86	138.0
87	108.0
88	77.0
89	26.0
90	6.0
91	4.5
92	3.0
93	2.5
94	2.0
95	3.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1068142.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.91696497811844
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.33181886453447	17.299575785866775
2	10.743819124632202	5.1391909147013495
3	4.2848487024011295	3.0744172905559215
4	2.1771345620855636	2.082818042962066
5	1.3364759935077175	1.5982224765410065
6	0.8998818042014751	1.2913464957319631
7	0.7146511381525085	1.196460036993622
8	0.5281465232756194	1.0105329520398403
9	0.4168656998826109	0.8973146110204113
>10	4.743761513093743	27.015173492891336
>50	1.5736848968193546	26.21599281397411
>100	0.23829411297416558	7.583511452981809
>500	0.005111919915287376	0.8742645559238401
>1k	0.004325470697550857	1.4135323015851244
>5k	3.9322460886825975E-4	0.6456353460001251
>10k+	7.864492177365195E-4	2.6620114302306686
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16333	1.5291038082951518	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	11972	1.1208247592548557	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	6865	0.6427048089111747	No Hit
GGGGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCT	2337	0.218791134512078	No Hit
CTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCTT	1889	0.17684914552559491	TruSeq Adapter, Index 22 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	1567	0.1467033409415602	TruSeq Adapter, Index 20 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGC	1359	0.12723027462640735	TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCGTCTTCTGCT	1339	0.1253578644037965	TruSeq Adapter, Index 20 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	1241	0.11618305431300334	No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCGTCAATCCTCG	1139	0.10663376217768798	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCAATCCTCGTATGCCG	1101	0.10307618275472735	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18377706334925506	0.0
2	0.0	0.0	0.0	0.5446841337574967	0.0
3	0.0	0.0	0.0	0.8788157379824031	0.0
4	0.0	0.0	0.0	1.2667791361073715	0.0
5	0.0	0.0	0.0	2.4909609396503463	0.0
6	0.0	0.0	0.0	3.732088055707949	0.0
7	0.0	0.0	0.0	4.723435648069264	0.0
8	0.0	0.0	0.0	6.432946181312971	0.0
9	0.0	0.0	0.0	7.4506947578131	0.0
10	0.0	0.0	0.0	8.468536954824359	0.0
11	0.0	0.0	0.0	9.868912560315014	0.0
12	0.0	0.0	0.0	10.675921366260292	0.0
13	0.0	0.0	0.0	11.075868189809968	0.0
14	0.0	0.0	0.0	11.446137311331265	0.0
15	0.0	0.0	0.0	11.73533107021351	0.0
16	9.362051113054257E-5	0.0	0.0	12.139865298808585	0.0
17	9.362051113054257E-5	0.0	0.0	12.610121126217301	0.0
18	9.362051113054257E-5	0.0	0.0	13.139451496149388	0.0
19	9.362051113054257E-5	0.0	0.0	13.460195367282628	0.0
20	9.362051113054257E-5	0.0	0.0	13.863606149744134	0.0
21	1.8724102226108514E-4	0.0	0.0	14.265706245049815	0.0
22	1.8724102226108514E-4	0.0	0.0	14.68578147849256	0.0
23	1.8724102226108514E-4	0.0	0.0	15.070749020261351	0.0
24	1.8724102226108514E-4	0.0	0.0	15.36406208163334	0.0
25	1.8724102226108514E-4	0.0	0.0	15.700346957614249	0.0
26	1.8724102226108514E-4	0.0	0.0	15.98514055247336	0.0
27	1.8724102226108514E-4	0.0	0.0	16.366082412263538	0.0
28	1.8724102226108514E-4	0.0	0.0	16.68785610901921	0.0
29	1.8724102226108514E-4	0.0	0.0	17.080500532700707	0.0
30	1.8724102226108514E-4	0.0	0.0	18.041515079455728	0.0
31	2.808615333916277E-4	0.0	0.0	18.466833061521783	0.0
32	2.808615333916277E-4	0.0	0.0	18.781866081476057	0.0
33	2.808615333916277E-4	0.0	0.0	19.151667100441703	0.0
34	2.808615333916277E-4	0.0	0.0	19.52568104240822	0.0
35	2.808615333916277E-4	0.0	0.0	19.885558287194026	0.0
36	2.808615333916277E-4	0.0	0.0	20.180743758788626	0.0
37	2.808615333916277E-4	0.0	0.0	20.530416367861203	0.0
38	2.808615333916277E-4	0.0	0.0	20.932610083678014	0.0
39	2.808615333916277E-4	0.0	0.0	22.132544174838177	0.0
40	2.808615333916277E-4	0.0	0.0	22.513205173094963	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATA	20	6.3112547E-4	46.0	42
TCGTTAG	45	3.092282E-10	46.0	1
CAAGTCG	20	6.3112547E-4	46.0	1
ACGTTTA	20	6.3112547E-4	46.0	15
ACGTTAG	40	5.6115823E-9	46.0	1
GCGAAAT	20	6.3112547E-4	46.0	30
GCAACGA	45	3.092282E-10	46.0	11
GTCTAGA	25	3.4169927E-5	46.0	9
CGACATA	20	6.3112547E-4	46.0	12
TACGCGA	20	6.3112547E-4	46.0	15
ACTACGG	30	1.8612791E-6	46.0	2
TATAGCG	30	1.8612791E-6	46.0	1
CGCAACG	20	6.3112547E-4	46.0	17
TTCGCAT	45	3.092282E-10	46.0	41
TAAGCGA	20	6.3112547E-4	46.0	38
TGTCGTA	25	3.4169927E-5	46.0	31
CGATAGT	20	6.3112547E-4	46.0	29
TGCGTAT	20	6.3112547E-4	46.0	44
CGTTAGG	140	0.0	44.35714	2
CGTTTTT	4250	0.0	43.456474	1
>>END_MODULE