FastQCFastQC Report
Sat 14 Jan 2017
SRR2933056.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933056.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327838
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC75740.5704009073396001No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51900.3908609333367474No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC44400.3343781395019573No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC33510.2523651228538421No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC21030.1583777539127514No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCTT20300.15288009531283184TruSeq Adapter, Index 10 (96% over 25bp)
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC18190.1369896026473109No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT17210.12960918425289833RNA PCR Primer, Index 10 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT16430.12373497369408015Illumina PCR Primer Index 2 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA13530.10189496007796132No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTACG351.01972546E-746.0000048
TCGTTAG253.417444E-546.026
ACTCTCG253.417444E-546.021
TTATCGT206.31181E-446.017
CATCGAT206.31181E-446.013
TGTACGA253.417444E-546.039
TATCGTG253.417444E-546.022
CGATATA253.417444E-546.019
TAAGACG206.31181E-446.015
ATAGCGG2050.044.878051
CGGTCTA2450.044.12244830
TACGCGC950.043.5789534
ATTGCGG2550.042.392161
CGTAGGG6700.041.8805962
TATCGCG554.7293724E-1141.81818432
GGACTAA16700.041.7305377
GTCGTTT1050.041.619058
TAGTAGG4050.041.4567871
ATACTCG508.712959E-1041.443
GGTACCT4750.041.1578987
Produced by FastQC (version 0.11.5)