##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933056.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1327838
Sequences flagged as poor quality	0
Sequence length	52
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55560542777056	33.0	31.0	34.0	31.0	34.0
2	32.93007505433645	34.0	31.0	34.0	31.0	34.0
3	33.035834943720545	34.0	33.0	34.0	31.0	34.0
4	36.42910430338641	37.0	37.0	37.0	35.0	37.0
5	36.40988659761206	37.0	37.0	37.0	35.0	37.0
6	36.52072240740211	37.0	37.0	37.0	35.0	37.0
7	36.54828073906606	37.0	37.0	37.0	35.0	37.0
8	36.39276553314485	37.0	37.0	37.0	35.0	37.0
9	38.07498279157548	39.0	39.0	39.0	35.0	39.0
10	37.96675196823709	39.0	38.0	39.0	35.0	39.0
11	37.553985501243375	39.0	37.0	39.0	35.0	39.0
12	37.418083380653364	39.0	37.0	39.0	35.0	39.0
13	37.42127202264132	39.0	37.0	39.0	35.0	39.0
14	38.81030743208132	41.0	38.0	41.0	35.0	41.0
15	38.862642882640806	41.0	38.0	41.0	35.0	41.0
16	38.8403547721936	41.0	38.0	41.0	35.0	41.0
17	38.84795735624376	41.0	37.0	41.0	35.0	41.0
18	38.781526059654865	40.0	37.0	41.0	35.0	41.0
19	38.69322010666964	40.0	37.0	41.0	35.0	41.0
20	38.579444179184506	40.0	36.0	41.0	35.0	41.0
21	38.513549845689006	40.0	36.0	41.0	35.0	41.0
22	38.46890885785766	40.0	35.0	41.0	35.0	41.0
23	38.424885415238904	40.0	35.0	41.0	35.0	41.0
24	38.37264109025348	40.0	35.0	41.0	35.0	41.0
25	38.30720238462825	40.0	35.0	41.0	35.0	41.0
26	38.214746829055954	40.0	35.0	41.0	35.0	41.0
27	38.17017964540855	40.0	36.0	41.0	35.0	41.0
28	38.18033148622046	40.0	36.0	41.0	35.0	41.0
29	38.17131909163618	40.0	36.0	41.0	35.0	41.0
30	38.098186676386725	40.0	35.0	41.0	35.0	41.0
31	38.01644251783727	40.0	35.0	41.0	34.0	41.0
32	37.94310676453001	40.0	35.0	41.0	34.0	41.0
33	37.80604862942618	40.0	35.0	41.0	34.0	41.0
34	37.6807185816342	40.0	35.0	41.0	34.0	41.0
35	37.565705304412134	40.0	35.0	41.0	34.0	41.0
36	37.4839159596276	40.0	35.0	41.0	33.0	41.0
37	37.387346950456305	40.0	35.0	41.0	33.0	41.0
38	37.36911656391819	40.0	35.0	41.0	33.0	41.0
39	37.27771234141514	40.0	35.0	41.0	33.0	41.0
40	37.25455138352721	40.0	35.0	41.0	33.0	41.0
41	37.20941410021403	40.0	35.0	41.0	33.0	41.0
42	37.149231306831105	39.0	35.0	41.0	33.0	41.0
43	37.06052394945769	39.0	35.0	41.0	33.0	41.0
44	36.97134590213565	39.0	35.0	41.0	33.0	41.0
45	36.91576984541789	39.0	35.0	41.0	33.0	41.0
46	36.82584095348981	39.0	35.0	41.0	33.0	41.0
47	36.74475952638801	39.0	35.0	41.0	33.0	41.0
48	36.67962432164165	38.0	35.0	41.0	33.0	41.0
49	36.58920289975133	38.0	35.0	41.0	33.0	41.0
50	36.50848597494574	38.0	35.0	41.0	33.0	41.0
51	36.40071906362071	38.0	35.0	41.0	32.0	41.0
52	36.15016816810484	37.0	35.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	3.0
14	9.0
15	21.0
16	46.0
17	100.0
18	175.0
19	319.0
20	608.0
21	994.0
22	1632.0
23	2556.0
24	3905.0
25	6121.0
26	8336.0
27	9962.0
28	10630.0
29	11824.0
30	13848.0
31	17016.0
32	21716.0
33	30098.0
34	72164.0
35	171867.0
36	73346.0
37	104746.0
38	168668.0
39	589689.0
40	7436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.23886196960774	21.52694831749054	24.22170475615248	19.012484956749244
2	27.966589297790843	23.503318928965733	26.78421614684924	21.745875626394184
3	29.067401294435015	24.121541935085457	25.319654957909023	21.49140181257051
4	24.97382963885655	27.890149250134428	23.162991268513178	23.973029842495848
5	22.628664038836064	38.12746735671068	21.260650772157447	17.983217832295807
6	87.54599582177947	3.3204351735678603	6.661354773699804	2.472214230952872
7	89.2652567557187	2.6974676127660153	5.244841614715049	2.792434016800242
8	82.08606772814153	4.7955398173572386	8.662276572895188	4.456115881606039
9	43.55456011953265	26.533055990263872	14.373741375077381	15.538642515126092
10	34.73149586018776	22.989551436244483	24.046834026439974	18.232118677127783
11	32.56617147573725	21.07757120974095	27.84722232681999	18.509034987701813
12	22.764222744039557	29.363597065304653	28.5917408599543	19.280439330701483
13	19.208593216943633	32.90680037775692	28.1230842919091	19.76152211339034
14	17.165874150310508	25.755250263962925	37.28278600250935	19.79608958321723
15	19.854379826454732	19.8901522625501	38.12980197885585	22.125665932139313
16	19.038692973088583	22.114745925331253	28.424777721378664	30.4217833802015
17	21.890697509786587	25.311220194029694	29.891824153247608	22.90625814293611
18	26.017932910490586	28.13001284795284	25.76082323295462	20.091231008601955
19	28.11110993961613	22.243376074491014	26.198150678019456	23.4473633078734
20	24.7393130788545	25.77678903601192	26.978366336857356	22.50553154827622
21	21.912236281835582	23.517251351444983	25.580002982291518	28.99050938442792
22	20.311513904557636	27.9259216862298	25.461238494454896	26.301325914757673
23	20.023451656000205	25.22084772389403	33.93990833219112	20.81579228791464
24	22.68341469365992	24.37066871109277	29.578532923443973	23.36738367180334
25	19.55652722696594	28.996609526162075	26.90546587761459	24.541397369257396
26	19.227270194105003	28.138221680656827	28.92062134085634	23.71388678438183
27	20.807282213643532	25.007192142414965	32.96290661963282	21.22261902430869
28	20.103732533637388	25.83613362473434	29.5758217493399	24.484312092288366
29	21.08449976578468	25.75901578355191	30.74471434015294	22.411770110510467
30	27.59146823633606	26.00513014388803	24.972172810237396	21.431228809538514
31	24.944458586062456	27.343169874638324	25.911971189256523	21.8004003500427
32	24.35959808350115	26.20982378874531	26.297786326344024	23.132791801409507
33	20.348566617313256	25.258276988608554	27.79766808902893	26.59548830504926
34	21.142790009022185	25.78191014265294	28.658616487854694	24.416683360470177
35	25.90150304479914	26.600157549339603	25.59423664633788	21.904102759523376
36	21.205071703024014	29.2806050135634	26.602642792268334	22.911680491144253
37	21.091804873787314	29.129532367653283	27.64772509899551	22.130937659563894
38	19.67800288890663	28.436526142496298	25.5243486027663	26.36112236583077
39	23.026076976257645	24.182618662818808	29.820731143407553	22.97057321751599
40	19.14970049057189	23.161033198326905	31.23498499063892	26.454281320462286
41	21.55097233246827	26.868262544075407	24.885565859690715	26.695199263765613
42	21.315175495805963	24.758743159933665	27.2682360348175	26.65784530944287
43	21.43439184599326	25.86942081790098	30.284417225595288	22.411770110510467
44	20.387426779471593	29.081710268873163	26.68442987774111	23.846433073914135
45	22.253317046205936	27.30114667602524	27.493790658197764	22.951745619571064
46	23.00039613266076	25.48676871726822	27.111741040699243	24.401094109371776
47	21.06002388845627	24.45418793557648	30.44339746264228	24.04239071332497
48	19.87177652695585	25.555301173787765	29.27375176791145	25.299170531344938
49	19.44017267166627	26.762978616367356	30.687629063183913	23.109219648782457
50	19.174854161426317	26.443286003262443	30.17725053809275	24.204609297218486
51	20.4828450458565	22.88200819678304	30.074527163705213	26.56061959365525
52	21.398544099506115	23.77345730427959	29.43363572965979	25.394362866554506
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1398.0
1	1962.5
2	2527.0
3	5559.0
4	8591.0
5	5737.0
6	2883.0
7	3109.5
8	3336.0
9	3318.0
10	3300.0
11	3414.5
12	3529.0
13	3592.0
14	3477.0
15	3299.0
16	3241.5
17	3184.0
18	3295.0
19	3406.0
20	3231.5
21	3057.0
22	3641.5
23	4226.0
24	4763.5
25	5301.0
26	6849.5
27	8398.0
28	10370.5
29	12343.0
30	13392.0
31	14441.0
32	16170.0
33	17899.0
34	23272.5
35	28646.0
36	31241.0
37	33836.0
38	35767.5
39	41428.0
40	45157.0
41	56915.0
42	68673.0
43	83229.5
44	97786.0
45	110806.5
46	123827.0
47	124208.5
48	124590.0
49	120957.0
50	117324.0
51	106361.5
52	95399.0
53	86362.0
54	77325.0
55	73609.5
56	69894.0
57	65213.0
58	60532.0
59	59096.0
60	57660.0
61	54553.0
62	51446.0
63	46771.0
64	35410.0
65	28724.0
66	24079.0
67	19434.0
68	16958.5
69	14483.0
70	12548.0
71	10613.0
72	9361.5
73	8110.0
74	6610.5
75	5111.0
76	3517.0
77	1923.0
78	1696.5
79	1470.0
80	1158.0
81	846.0
82	563.0
83	280.0
84	169.5
85	59.0
86	81.5
87	104.0
88	59.0
89	8.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1327838.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.749169611392446
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.01840100447187	14.420527861899835
2	10.743039306444512	4.24332210809657
3	3.955710704171591	2.343660048908562
4	1.8805643185451475	1.4855833476832307
5	1.0934857471088066	1.0797717743646003
6	0.7485092605034411	0.8869461804828234
7	0.6109088800749658	0.8445460162794415
8	0.39828625860905653	0.6292658300125737
9	0.35356606224364556	0.6284372518873707
>10	4.480308225880453	21.70379647731237
>50	1.9046974541503832	27.28180215225926
>100	0.8021794892527787	21.129299863504524
>500	0.005746271412985509	0.783418492796105
>1k	0.0038308476086570064	1.573948655155014
>5k	7.661695217314012E-4	0.9656739393577484
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	7574	0.5704009073396001	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5190	0.3908609333367474	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	4440	0.3343781395019573	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3351	0.2523651228538421	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2103	0.1583777539127514	No Hit
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCTT	2030	0.15288009531283184	TruSeq Adapter, Index 10 (96% over 25bp)
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	1819	0.1369896026473109	No Hit
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	1721	0.12960918425289833	RNA PCR Primer, Index 10 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	1643	0.12373497369408015	Illumina PCR Primer Index 2 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1353	0.10189496007796132	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15785058117029335	0.0
2	0.0	0.0	0.0	0.496446102611915	0.0
3	0.0	0.0	0.0	0.8582372247216904	0.0
4	0.0	0.0	0.0	1.1130875905042634	0.0
5	0.0	0.0	0.0	2.4390776585697953	0.0
6	0.0	0.0	0.0	2.6970910608071166	0.0
7	0.0	0.0	0.0	3.5005023203131707	0.0
8	7.531039177972011E-5	0.0	0.0	4.471855753488001	0.0
9	7.531039177972011E-5	0.0	0.0	5.352836716527166	0.0
10	7.531039177972011E-5	0.0	0.0	5.947487570019836	0.0
11	7.531039177972011E-5	0.0	0.0	6.567141473583374	0.0
12	7.531039177972011E-5	0.0	0.0	7.062985093061051	0.0
13	7.531039177972011E-5	0.0	0.0	7.251863555644589	0.0
14	7.531039177972011E-5	0.0	0.0	7.384334534785117	0.0
15	7.531039177972011E-5	0.0	0.0	7.840338957011322	0.0
16	7.531039177972011E-5	0.0	0.0	8.447566645931206	0.0
17	7.531039177972011E-5	0.0	0.0	9.19457042199425	7.531039177972011E-5
18	7.531039177972011E-5	0.0	0.0	9.536780842241297	7.531039177972011E-5
19	7.531039177972011E-5	0.0	0.0	9.87537636368292	7.531039177972011E-5
20	7.531039177972011E-5	0.0	0.0	10.307808633282072	7.531039177972011E-5
21	7.531039177972012E-4	0.0	0.0	10.740768075623683	7.531039177972011E-5
22	8.284143095769213E-4	0.0	0.0	11.228252241613811	7.531039177972011E-5
23	8.284143095769213E-4	0.0	0.0	11.61579951771225	7.531039177972011E-5
24	8.284143095769213E-4	0.0	0.0	11.891887413976704	7.531039177972011E-5
25	9.037247013566414E-4	0.0	0.0	12.187104149753209	7.531039177972011E-5
26	9.037247013566414E-4	0.0	0.0	12.46093273426427	7.531039177972011E-5
27	9.037247013566414E-4	0.0	0.0	12.780474726585622	7.531039177972011E-5
28	9.037247013566414E-4	0.0	0.0	13.133002670506492	7.531039177972011E-5
29	9.037247013566414E-4	0.0	0.0	13.48040950778634	7.531039177972011E-5
30	9.037247013566414E-4	0.0	0.0	13.815691371989656	7.531039177972011E-5
31	9.037247013566414E-4	0.0	0.0	14.121828114574217	7.531039177972011E-5
32	9.037247013566414E-4	0.0	0.0	14.460800187974739	7.531039177972011E-5
33	9.037247013566414E-4	0.0	0.0	14.775597625613967	7.531039177972011E-5
34	9.037247013566414E-4	0.0	0.0	15.184533052977848	7.531039177972011E-5
35	9.037247013566414E-4	0.0	0.0	15.504903459608778	7.531039177972011E-5
36	9.037247013566414E-4	0.0	0.0	15.824746693497248	7.531039177972011E-5
37	0.0010543454849160816	0.0	0.0	16.161233523969038	7.531039177972011E-5
38	0.0010543454849160816	0.0	0.0	16.53206189309238	7.531039177972011E-5
39	0.0010543454849160816	0.0	0.0	16.902137158297926	7.531039177972011E-5
40	0.0010543454849160816	0.0	0.0	17.248866202051758	7.531039177972011E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTACG	35	1.01972546E-7	46.000004	8
TCGTTAG	25	3.417444E-5	46.0	26
ACTCTCG	25	3.417444E-5	46.0	21
TTATCGT	20	6.31181E-4	46.0	17
CATCGAT	20	6.31181E-4	46.0	13
TGTACGA	25	3.417444E-5	46.0	39
TATCGTG	25	3.417444E-5	46.0	22
CGATATA	25	3.417444E-5	46.0	19
TAAGACG	20	6.31181E-4	46.0	15
ATAGCGG	205	0.0	44.87805	1
CGGTCTA	245	0.0	44.122448	30
TACGCGC	95	0.0	43.57895	34
ATTGCGG	255	0.0	42.39216	1
CGTAGGG	670	0.0	41.880596	2
TATCGCG	55	4.7293724E-11	41.818184	32
GGACTAA	1670	0.0	41.730537	7
GTCGTTT	105	0.0	41.61905	8
TAGTAGG	405	0.0	41.456787	1
ATACTCG	50	8.712959E-10	41.4	43
GGTACCT	475	0.0	41.157898	7
>>END_MODULE