##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933055.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1492037
Sequences flagged as poor quality	0
Sequence length	52
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50948736525971	33.0	31.0	34.0	31.0	34.0
2	32.86032786050212	34.0	31.0	34.0	31.0	34.0
3	32.863916913588604	34.0	31.0	34.0	31.0	34.0
4	36.3591914945809	37.0	37.0	37.0	35.0	37.0
5	36.3561962605485	37.0	37.0	37.0	35.0	37.0
6	36.48367902404565	37.0	37.0	37.0	35.0	37.0
7	36.52660624367894	37.0	37.0	37.0	35.0	37.0
8	36.393540508713926	37.0	37.0	37.0	35.0	37.0
9	38.083913468633824	39.0	39.0	39.0	35.0	39.0
10	37.9965650985867	39.0	39.0	39.0	35.0	39.0
11	37.75849928654584	39.0	38.0	39.0	35.0	39.0
12	37.66447547882526	39.0	38.0	39.0	35.0	39.0
13	37.50204720124233	39.0	38.0	39.0	35.0	39.0
14	39.06704391379034	41.0	39.0	41.0	35.0	41.0
15	39.14900434774741	41.0	39.0	41.0	35.0	41.0
16	39.139921463073634	41.0	39.0	41.0	35.0	41.0
17	39.123776421094114	41.0	38.0	41.0	35.0	41.0
18	38.92031698945804	40.0	38.0	41.0	35.0	41.0
19	38.68209233417134	40.0	37.0	41.0	35.0	41.0
20	38.456431040249	40.0	35.0	41.0	35.0	41.0
21	38.39943781555015	40.0	35.0	41.0	35.0	41.0
22	38.344753514825705	40.0	35.0	41.0	35.0	41.0
23	38.28013916544965	40.0	35.0	41.0	35.0	41.0
24	38.19817135902126	40.0	35.0	41.0	35.0	41.0
25	38.13028363237641	40.0	35.0	41.0	35.0	41.0
26	38.024523520529314	40.0	35.0	41.0	34.0	41.0
27	37.93032210327224	40.0	35.0	41.0	34.0	41.0
28	37.85842911402331	40.0	35.0	41.0	34.0	41.0
29	37.7767474935273	40.0	35.0	41.0	34.0	41.0
30	37.649225186774856	40.0	35.0	41.0	34.0	41.0
31	37.503762976387314	40.0	35.0	41.0	33.0	41.0
32	37.26890351914865	40.0	35.0	41.0	33.0	41.0
33	36.91618907574008	40.0	35.0	41.0	33.0	41.0
34	36.61387552721548	40.0	35.0	41.0	31.0	41.0
35	36.372750139574286	39.0	35.0	41.0	31.0	41.0
36	36.22555137707711	39.0	35.0	41.0	30.0	41.0
37	36.07733521353693	39.0	35.0	41.0	30.0	41.0
38	36.009441454870085	39.0	35.0	41.0	30.0	41.0
39	35.909866846465604	39.0	35.0	41.0	29.0	41.0
40	35.820162636717455	39.0	35.0	41.0	29.0	41.0
41	35.75394578016497	39.0	35.0	41.0	29.0	41.0
42	35.68573031365844	38.0	35.0	41.0	29.0	41.0
43	35.57188595188993	38.0	35.0	41.0	28.0	41.0
44	35.45048614746149	38.0	35.0	41.0	28.0	41.0
45	35.388074826562615	38.0	35.0	41.0	28.0	41.0
46	35.31330389259784	37.0	35.0	41.0	27.0	41.0
47	35.20507132195783	37.0	35.0	41.0	27.0	41.0
48	35.11522636502982	37.0	35.0	40.0	26.0	41.0
49	35.043756957769816	37.0	35.0	40.0	27.0	41.0
50	34.944794264485395	37.0	35.0	40.0	26.0	41.0
51	34.817634549277265	36.0	35.0	40.0	26.0	41.0
52	34.58688893103857	36.0	35.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	4.0
13	5.0
14	11.0
15	33.0
16	61.0
17	182.0
18	301.0
19	598.0
20	1039.0
21	1669.0
22	2714.0
23	4576.0
24	8189.0
25	15221.0
26	22993.0
27	25185.0
28	23990.0
29	21760.0
30	21635.0
31	23962.0
32	28638.0
33	37942.0
34	83251.0
35	119498.0
36	126027.0
37	142142.0
38	207952.0
39	564340.0
40	8116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.36427782957125	19.778866073696562	21.10503962033113	26.751816476401054
2	36.55036704853834	20.932590813766684	22.63683809449766	19.88020404319732
3	26.696656986388405	21.774661084142018	32.68203134372673	18.846650585742847
4	21.61132733303531	25.357212991366833	30.34120467521918	22.690255000378677
5	20.593390110298873	35.838923565568415	27.32908098123572	16.238605342896992
6	79.72563683072202	2.9251955548019253	14.77852090799357	2.57064670648248
7	80.33219015346134	1.9363460825703382	13.48994696512218	4.241516798846141
8	74.20928569465771	3.8422639653038098	17.463574964964003	4.484875375074479
9	35.84542474482871	27.51540343838658	21.999052302322262	14.640119514462443
10	27.72880297204426	20.105801665776386	33.113656028637365	19.051739333542
11	25.878245646723236	18.198744401110698	36.65029754624047	19.272712405925592
12	22.557215404175636	18.81481491410736	38.94172865686307	19.686241024853942
13	18.29465355081677	24.13606364989608	36.92140342364164	20.647879375645513
14	16.468023246072317	24.289880210745444	38.96237157657618	20.279724966606054
15	20.26491300148723	19.630411310175283	40.348731298218475	19.755944390119012
16	20.666779711227	22.772357521964935	34.826549207559864	21.734313559248193
17	19.935899712942774	26.66502238215272	34.15009145215568	19.248986452748827
18	21.73659232311263	31.301033419412523	29.737533318543708	17.224840938931138
19	20.153856774329324	27.313062611718074	30.321366025105274	22.21171458884733
20	22.468477658395873	27.0672242042255	32.32064620381398	18.14365193356465
21	18.72962935905745	24.371312507665692	31.47267795637776	25.426380176899098
22	18.236411027340473	29.534321199809387	31.488428236028998	20.740839536821138
23	17.238848634450754	30.61271268741995	32.02514414857004	20.123294529559253
24	23.600219029420852	26.292645557717403	29.793497078155568	20.313638334706177
25	18.830967328558206	25.61129516225134	30.74360756469176	24.814129944498696
26	18.18641226725611	24.247656056786795	35.021115428102654	22.544816247854442
27	20.239980643911647	23.88787945607247	34.659462198323496	21.212677701692385
28	21.169515233201324	28.430260107490636	29.373400257500315	21.02682440180773
29	21.903009107682987	24.863726569783456	33.058831650957714	20.17443267157584
30	23.97514270758701	23.76542940959239	29.435261994173068	22.82416588864753
31	23.712146548644572	26.496594923584333	28.859270916203823	20.931987611567273
32	21.35818347668322	24.616547712958862	28.753911598707006	25.27135721165092
33	19.03297304289371	25.867119917267466	31.24835376066411	23.851553279174713
34	19.293020213305702	26.56804087298103	28.870463668126195	25.268475245587073
35	25.27933288517644	28.482403586506233	26.758853835394163	19.479409692923163
36	20.42523074159689	27.912645597930887	26.836935008984362	24.825188651487863
37	22.526049957206155	29.817223031332336	26.53788076301057	21.118846248450943
38	19.074258882319942	26.077905574727705	25.450776354741873	29.397059188210477
39	21.45268515459067	25.889706488512015	30.3099051833165	22.347703173580815
40	19.105156239423017	24.015691299880633	31.972397467355034	24.906754993341316
41	19.491004579645143	29.081182303119828	28.88701821737665	22.540794899858383
42	19.344895602454898	24.987986222861768	30.10856969364701	25.55854848103633
43	22.914177061292715	26.237553090171357	31.23193325634686	19.616336592189068
44	22.17143408642011	26.051364677953696	29.41053070399729	22.366670531628905
45	22.668137586400334	26.31764493775959	29.014696016251605	21.99952145958847
46	23.20485349894138	25.702646784228538	29.01208214005417	22.08041757677591
47	20.86938862776191	29.160469881108845	28.03670418360939	21.933437307519853
48	21.116366417186704	26.9955101649624	27.229485595866592	24.658637821984307
49	19.20428246752594	24.67887860689782	31.704240578484317	24.41259834709193
50	19.308770492956945	25.62496774543795	32.74402712533268	22.322234636272427
51	18.931098893660145	24.515745923190913	31.407129984041948	25.14602519910699
52	18.131990024376073	24.183850668582615	30.937503560568537	26.746655746472776
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1564.0
1	2033.5
2	2503.0
3	32128.5
4	61754.0
5	36585.5
6	11417.0
7	11714.0
8	12011.0
9	13796.5
10	15582.0
11	17296.0
12	19010.0
13	19646.5
14	19238.5
15	18194.0
16	16256.5
17	14319.0
18	13174.0
19	12029.0
20	11147.0
21	10265.0
22	9731.5
23	9198.0
24	9317.5
25	9437.0
26	9990.0
27	10543.0
28	10945.5
29	11348.0
30	13845.5
31	16343.0
32	16539.0
33	16735.0
34	18954.5
35	21174.0
36	23597.5
37	26021.0
38	28796.5
39	32422.5
40	33273.0
41	37156.5
42	41040.0
43	48127.0
44	55214.0
45	68789.5
46	82365.0
47	97681.0
48	112997.0
49	132133.5
50	151270.0
51	140396.0
52	129522.0
53	110904.0
54	92286.0
55	83320.0
56	74354.0
57	70838.0
58	67322.0
59	66196.5
60	65071.0
61	60894.0
62	56717.0
63	53895.0
64	45466.0
65	39859.0
66	34287.0
67	28715.0
68	24311.5
69	19908.0
70	17052.5
71	14197.0
72	12901.5
73	11606.0
74	9102.5
75	6599.0
76	5161.0
77	3723.0
78	2752.5
79	1782.0
80	1437.0
81	1092.0
82	776.0
83	460.0
84	325.5
85	191.0
86	124.5
87	58.0
88	40.0
89	19.5
90	17.0
91	9.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1492037.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.612152705828453
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.08759490994646	21.346688686692364
2	9.307917892649764	5.512549720209155
3	4.069757791547564	3.615428675971249
4	2.339600615031747	2.7712244273188107
5	1.5899691487292182	2.3541204614862843
6	1.165238211500237	2.0703127114566873
7	0.9615379994879726	1.993124705130625
8	0.7868723729845135	1.8640787895052018
9	0.7119675052649809	1.8974601438744902
>10	6.677136528951268	39.09836272552095
>50	0.2220445475331217	4.354224246750449
>100	0.07011796981402978	3.504369607356237
>500	0.004553114026249089	0.9278154951482301
>1k	0.004325458324936635	2.631765539133311
>5k	9.106228052498178E-4	1.8678328797552868
>10k+	4.553114026249089E-4	4.190641184690796
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	49817	3.3388582186634785	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	12346	0.8274593726563081	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	8364	0.5605759106510093	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	7797	0.5225741720882257	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTC	6074	0.40709446213465217	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTT	5472	0.3667469372408325	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCT	4099	0.27472509059761924	No Hit
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3804	0.25495346295031557	No Hit
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3211	0.21520914025590518	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAAT	3140	0.2104505451272321	No Hit
CGCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTGC	2928	0.19624178220781388	TruSeq Adapter, Index 16 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGA	2900	0.19436515314298505	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCT	2307	0.15462083044857466	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTC	2004	0.13431302306846277	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCATGCACTCGTATGCCGTCTTCTG	1670	0.11192751922371898	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09061437484459166	0.0
2	0.0	0.0	0.0	0.2589748109463773	0.0
3	0.0	0.0	0.0	0.616941805062475	0.0
4	0.0	0.0	0.0	0.8972297603879796	0.0
5	0.0	0.0	0.0	2.4319102006183493	0.0
6	0.0	0.0	0.0	3.679265326530106	0.0
7	0.0	0.0	0.0	5.98316261594049	0.0
8	0.0	0.0	0.0	8.089343628877836	0.0
9	0.0	0.0	0.0	10.685592917601909	0.0
10	0.0	0.0	0.0	11.926111751920361	0.0
11	6.702246660102934E-5	0.0	0.0	12.657930064737	0.0
12	6.702246660102934E-5	0.0	0.0	13.167032721038419	0.0
13	6.702246660102934E-5	0.0	0.0	13.618429033596351	0.0
14	6.702246660102934E-5	0.0	0.0	13.815743175269782	0.0
15	1.3404493320205867E-4	0.0	0.0	14.081018098076656	0.0
16	1.3404493320205867E-4	0.0	0.0	14.341467403288256	0.0
17	1.3404493320205867E-4	0.0	0.0	14.725573159378756	0.0
18	1.3404493320205867E-4	0.0	0.0	14.91015303239799	0.0
19	1.3404493320205867E-4	0.0	0.0	15.128780318450548	0.0
20	1.3404493320205867E-4	0.0	0.0	15.352501311964783	0.0
21	1.3404493320205867E-4	0.0	0.0	15.609063314113524	0.0
22	1.3404493320205867E-4	0.0	0.0	15.861335878399798	0.0
23	2.01067399803088E-4	0.0	0.0	16.080365299251962	0.0
24	2.01067399803088E-4	0.0	0.0	16.254154555148432	0.0
25	2.01067399803088E-4	0.0	0.0	16.451200606955457	0.0
26	2.01067399803088E-4	0.0	0.0	16.675189690336097	0.0
27	2.01067399803088E-4	0.0	0.0	16.905411863110633	0.0
28	2.01067399803088E-4	0.0	0.0	17.13496381121916	0.0
29	2.01067399803088E-4	0.0	0.0	17.347022895544814	0.0
30	2.01067399803088E-4	0.0	0.0	17.568867259994224	0.0
31	2.01067399803088E-4	0.0	0.0	17.76068555940637	0.0
32	2.01067399803088E-4	0.0	0.0	18.006322899499143	0.0
33	2.01067399803088E-4	0.0	0.0	18.249949565593884	0.0
34	2.01067399803088E-4	0.0	0.0	18.59089285319332	0.0
35	2.01067399803088E-4	0.0	0.0	18.81220103790992	0.0
36	2.6808986640411734E-4	0.0	0.0	19.06025118680033	0.0
37	2.6808986640411734E-4	0.0	0.0	19.382495206218078	0.0
38	2.6808986640411734E-4	0.0	0.0	20.070413803411043	0.0
39	2.6808986640411734E-4	0.0	0.0	20.41403798967452	0.0
40	3.3511233300514666E-4	0.0	0.0	20.782460488580377	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15205	0.0	45.10753	1
ACTACGG	85	0.0	43.294117	1
TATACGG	85	0.0	43.294117	1
TACGGGA	510	0.0	42.843132	3
CGGTCTA	700	0.0	42.714287	30
TCACGAC	695	0.0	42.690647	24
CACGACG	705	0.0	42.411346	25
CGCACTT	4960	0.0	42.336693	34
CATGCGG	185	0.0	42.27027	1
ACTTACT	4935	0.0	42.224922	37
ACGGGTA	115	0.0	42.0	4
CCGCACT	5000	0.0	41.998	33
CACTTAC	4990	0.0	41.98998	36
CCCGCAC	5085	0.0	41.974434	32
GCACTTA	5005	0.0	41.910088	35
CTTACTG	4975	0.0	41.885426	38
CGACGGT	725	0.0	41.875866	27
TTTACGG	55	4.7293724E-11	41.818184	1
GTAACGG	110	0.0	41.818184	1
TTATGAC	5320	0.0	41.633457	26
>>END_MODULE