FastQCFastQC Report
Sat 14 Jan 2017
SRR2933053.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933053.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2770693
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT771152.7832387059843873No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC149570.5398288442638719No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC73220.2642660157585124No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT69270.2500096546243124No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC62230.22460084895728252No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC59990.21651622897231848No Hit
CGTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT51020.18414165697895796TruSeq Adapter, Index 15 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC35260.12726058065617518TruSeq Adapter, Index 15 (95% over 23bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC32340.11672170103291848No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCT31410.11336514005701823No Hit
CCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGCT27760.10019154052794733TruSeq Adapter, Index 21 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT276550.044.6111031
CGCACTT47100.043.02123334
CCGCACT47450.042.75237333
CCCGCAC48300.042.6190532
TCAACGC51150.042.22287415
ACCCGCA48900.042.04908431
CGGTCTA7300.041.9041130
AACGCAA51400.041.8385217
CAACGCA51550.041.8060116
TATGCGG4900.041.7755131
TACGGGA10050.041.6517453
ACGCAAG51750.041.55555718
TCACGAC7650.041.49019624
GCTTATG50900.041.48133524
CGCAAGC51850.041.4754119
CTAACGG2000.041.3999981
GCACTTA48750.041.3764135
ATCAACG52250.041.3339714
TTATGAC51400.041.21206326
CGACGGT7550.041.1258327
Produced by FastQC (version 0.11.5)