FastQCFastQC Report
Sat 14 Jan 2017
SRR2933052.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933052.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000500
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199931.9983008495752124No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC77970.7793103448275862No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC56450.5642178910544727No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC54800.5477261369315342No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCTT31630.31614192903548227Illumina Single End Adapter 2 (95% over 24bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC31120.31104447776111943No Hit
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT24270.24257871064467768TruSeq Adapter, Index 22 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT23150.23138430784607694TruSeq Adapter, Index 22 (96% over 25bp)
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC22780.22768615692153926No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG21770.21759120439780108No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15960.15952023988005998No Hit
ACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC14420.144127936031984Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC12050.12043978010994504Illumina Single End Adapter 2 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC11010.11004497751124437No Hit
GCCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC10840.10834582708645678TruSeq Adapter, Index 22 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCG351.01939804E-746.00000431
CCATCGT351.01939804E-746.00000441
GATATCG351.01939804E-746.00000410
CTTCGAG253.416837E-546.028
TTAGGCG253.416837E-546.01
CGAACGT253.416837E-546.037
CGTATCA206.311062E-446.043
CGCGGAT206.311062E-446.03
TCCGAAC253.416837E-546.035
TTTCGTG206.311062E-446.031
GACGTTA206.311062E-446.019
CAGTTCG206.311062E-446.012
TAGCGCC253.416837E-546.033
TTCACGG650.046.01
CTCCCGC206.311062E-446.037
ATTCGTT206.311062E-446.042
CGTTAAT206.311062E-446.021
GGGCGTA551.8189894E-1246.06
ACTACGG650.046.01
GGACGCG253.416837E-546.07
Produced by FastQC (version 0.11.5)