##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933052.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1000500
Sequences flagged as poor quality	0
Sequence length	52
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47639780109945	33.0	31.0	34.0	31.0	34.0
2	32.786659670164916	34.0	31.0	34.0	31.0	34.0
3	32.80941329335332	34.0	31.0	34.0	31.0	34.0
4	36.29526936531734	37.0	37.0	37.0	35.0	37.0
5	36.276185907046475	37.0	37.0	37.0	35.0	37.0
6	36.45296151924038	37.0	37.0	37.0	35.0	37.0
7	36.54228985507246	37.0	37.0	37.0	35.0	37.0
8	36.38382808595702	37.0	37.0	37.0	35.0	37.0
9	37.99967416291854	39.0	38.0	39.0	35.0	39.0
10	37.86590004997501	39.0	38.0	39.0	35.0	39.0
11	37.37070964517741	39.0	35.0	39.0	35.0	39.0
12	37.18306646676662	39.0	35.0	39.0	34.0	39.0
13	36.86403998001	39.0	35.0	39.0	33.0	39.0
14	38.38262668665667	40.0	37.0	41.0	33.0	41.0
15	38.54699850074962	40.0	37.0	41.0	35.0	41.0
16	38.602757621189404	40.0	36.0	41.0	35.0	41.0
17	38.643988005997	40.0	36.0	41.0	35.0	41.0
18	38.53256371814093	40.0	36.0	41.0	35.0	41.0
19	38.35090554722639	40.0	36.0	41.0	35.0	41.0
20	38.23767516241879	40.0	35.0	41.0	35.0	41.0
21	38.2375952023988	40.0	35.0	41.0	35.0	41.0
22	38.20181209395302	40.0	35.0	41.0	35.0	41.0
23	38.12782408795602	40.0	35.0	41.0	34.0	41.0
24	38.03943828085957	40.0	35.0	41.0	34.0	41.0
25	37.97209295352324	40.0	35.0	41.0	34.0	41.0
26	37.901416291854076	40.0	35.0	41.0	34.0	41.0
27	37.82767816091954	40.0	35.0	41.0	34.0	41.0
28	37.79918740629685	40.0	35.0	41.0	34.0	41.0
29	37.731167416291854	40.0	35.0	41.0	34.0	41.0
30	37.595647176411795	40.0	35.0	41.0	34.0	41.0
31	37.49985707146427	40.0	35.0	41.0	34.0	41.0
32	37.396354822588705	40.0	35.0	41.0	34.0	41.0
33	37.192708645677165	39.0	35.0	41.0	33.0	41.0
34	37.010980509745124	39.0	35.0	41.0	33.0	41.0
35	36.88931634182909	39.0	35.0	41.0	33.0	41.0
36	36.79345427286357	39.0	35.0	41.0	33.0	41.0
37	36.68384607696152	39.0	35.0	41.0	32.0	41.0
38	36.69944527736132	39.0	35.0	41.0	33.0	41.0
39	36.601790104947526	39.0	35.0	41.0	32.0	41.0
40	36.514854572713645	39.0	35.0	41.0	32.0	41.0
41	36.52053773113443	39.0	35.0	41.0	32.0	41.0
42	36.496763618190904	39.0	35.0	41.0	32.0	41.0
43	36.41128535732134	38.0	35.0	41.0	32.0	41.0
44	36.32197101449275	38.0	35.0	41.0	32.0	41.0
45	36.26042678660669	38.0	35.0	41.0	32.0	41.0
46	36.159022488755625	38.0	35.0	41.0	31.0	41.0
47	36.03664467766117	38.0	35.0	41.0	31.0	41.0
48	35.99688155922039	38.0	35.0	41.0	31.0	41.0
49	35.920286856571714	37.0	35.0	41.0	31.0	41.0
50	35.79860469765117	37.0	35.0	40.0	31.0	41.0
51	35.69268165917042	37.0	35.0	40.0	30.0	41.0
52	35.439264367816094	37.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	0.0
12	2.0
13	3.0
14	2.0
15	10.0
16	14.0
17	78.0
18	193.0
19	291.0
20	557.0
21	854.0
22	1414.0
23	2363.0
24	4231.0
25	7158.0
26	9410.0
27	10395.0
28	10379.0
29	10999.0
30	12686.0
31	15725.0
32	20298.0
33	28579.0
34	70421.0
35	126109.0
36	77994.0
37	79142.0
38	117734.0
39	387508.0
40	5949.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.580509745127436	22.818290854572716	25.26166916541729	18.33953023488256
2	30.578010994502748	23.04297851074463	26.057471264367816	20.321539230384808
3	26.981709145427285	24.186606696651676	28.305647176411796	20.526036981509247
4	22.886356821589203	26.98150924537731	27.2031984007996	22.928935532233883
5	20.771714142928534	37.45537231384308	23.832183908045977	17.94072963518241
6	84.17251374312843	2.4642678660669666	11.057371314342829	2.305847076461769
7	86.74192903548226	1.8009995002498753	8.135932033983009	3.3211394302848576
8	78.72983508245876	3.9258370814592705	12.724737631184407	4.619590204897552
9	40.583108445777114	29.664767616191906	16.27656171914043	13.475562218890555
10	34.06926536731634	20.33373313343328	28.484657671164413	17.112343828085958
11	32.126336831584204	20.14942528735632	30.24247876061969	17.48175912043978
12	23.39480259870065	28.714442778610692	32.84977511244378	15.040979510244878
13	16.344127936031985	34.92823588205897	33.44457771114443	15.283058470764619
14	14.522138930534734	30.30064967516242	39.042578710644676	16.13463268365817
15	18.785007496251875	22.90494752623688	40.52883558220889	17.78120939530235
16	18.906746626686658	25.77131434282859	27.691254372813596	27.63068465767116
17	21.291254372813594	29.27626186906547	29.675462268865566	19.757021489255372
18	23.33743128435782	33.645577211394304	26.822288855572214	16.194702648675662
19	20.00759620189905	30.328435782108947	26.22278860569715	23.44117941029485
20	19.465167416291855	30.241779110444778	31.026986506746628	19.266066966516743
21	16.449175412293854	26.554822588705644	31.470164917541226	25.52583708145927
22	13.96601699150425	32.43228385807097	28.75162418790605	24.85007496251874
23	14.364617691154422	34.874362818590704	32.224787606196905	18.536231884057973
24	22.736531734132935	28.368115942028986	28.320839580209896	20.574512743628187
25	14.506246876561718	31.789105447276363	26.612493753123438	27.09215392303848
26	13.964217891054473	30.419590204897553	33.44177911044478	22.1744127936032
27	17.797401299350323	28.40209895052474	33.77051474262869	20.02998500749625
28	14.947326336831585	33.33753123438281	28.267666166916545	23.447476261869067
29	19.89735132433783	28.772913543228384	32.43008495752124	18.899650174912544
30	27.453073463268368	28.176511744127936	25.806396801599202	18.564017991004498
31	23.769415292353823	32.78270864567716	24.822588705647178	18.62528735632184
32	22.962418790604698	27.867666166916543	23.896451774112943	25.273463268365816
33	20.07936031984008	27.263968015992003	28.405597201399303	24.251074462768614
34	16.636781609195403	29.730434782608693	28.549625187406296	25.0831584207896
35	30.303948025987005	27.96531734132934	23.343228385807098	18.387506246876562
36	19.90104947526237	31.420689655172414	24.634682658670666	24.04357821089455
37	21.869965017491253	31.53233383308346	26.45957021489255	20.138130934532732
38	19.22838580709645	27.48165917041479	24.574912543728136	28.715042478760623
39	24.894652673663167	25.285657171414293	28.263668165917043	21.556021989005497
40	18.269065467266365	23.696451774112944	32.4063968015992	25.62808595702149
41	19.411794102948527	30.6023988005997	26.636981509245377	23.348825587206395
42	19.857471264367817	25.501549225387304	28.600199900049976	26.040779610194903
43	21.866266866566715	28.249675162418793	28.781209395302348	21.102848575712144
44	19.35232383808096	30.806196901549228	24.2367816091954	25.604697651174412
45	22.777011494252875	27.69535232383808	26.647776111944026	22.879860069965016
46	24.05417291354323	27.528235882058972	28.076861569215396	20.34072963518241
47	19.13943028485757	31.225087456271865	28.455372313843082	21.180109945027485
48	20.267866066966516	27.427786106946527	26.93053473263368	25.373813093453272
49	18.760919540229885	27.386206896551723	30.269765117441278	23.583108445777114
50	18.257971014492753	28.065267366316842	30.318540729635185	23.358220889555223
51	18.758920539730134	26.190904547726134	27.490454772613692	27.559720139930032
52	19.512043978010997	24.464667666166918	30.599600199900053	25.423688155922036
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1722.0
1	1611.0
2	1500.0
3	11641.0
4	21782.0
5	12426.5
6	3071.0
7	3780.5
8	4490.0
9	6262.5
10	8035.0
11	9747.5
12	11460.0
13	11867.5
14	11689.5
15	11104.0
16	9564.5
17	8025.0
18	7068.5
19	6112.0
20	5680.0
21	5248.0
22	4963.0
23	4678.0
24	4864.0
25	5050.0
26	5349.0
27	5648.0
28	6335.5
29	7023.0
30	7880.0
31	8737.0
32	10069.0
33	11401.0
34	13370.5
35	15340.0
36	17203.0
37	19066.0
38	20667.5
39	23567.0
40	24865.0
41	32464.0
42	40063.0
43	50660.5
44	61258.0
45	67331.0
46	73404.0
47	78785.5
48	84167.0
49	106492.5
50	128818.0
51	125735.5
52	122653.0
53	105426.0
54	88199.0
55	74036.5
56	59874.0
57	52081.5
58	44289.0
59	38381.0
60	32473.0
61	25107.0
62	17741.0
63	15136.0
64	9473.5
65	6416.0
66	5079.5
67	3743.0
68	2720.0
69	1697.0
70	1660.5
71	1624.0
72	1523.0
73	1422.0
74	1012.5
75	603.0
76	486.0
77	369.0
78	245.0
79	121.0
80	89.5
81	58.0
82	39.0
83	20.0
84	35.0
85	50.0
86	27.0
87	4.0
88	2.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1000500.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.322326609140042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.85841182308904	21.789142329137963
2	12.07942031702491	7.325522562038225
3	4.78828850957937	4.355761442587742
4	2.42774425954221	2.944594174452149
5	1.4895071424245903	2.258266102962265
6	0.953140622705623	1.7340864759671413
7	0.7084685037658847	1.503768935443454
8	0.540309934343526	1.3106763439461926
9	0.4077728198565507	1.1128158563418322
>10	3.8378435605622387	24.722834649584584
>50	0.7458458394365346	15.770923988010347
>100	0.14969785517059245	7.346869971206675
>500	0.008591929877335426	1.7309033262762212
>1k	0.003635047255795757	2.194441521677043
>5k	9.913765243079339E-4	1.896037555852649
>10k+	3.3045884143597786E-4	2.0033547645154854
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19993	1.9983008495752124	No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	7797	0.7793103448275862	No Hit
GTGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5645	0.5642178910544727	No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	5480	0.5477261369315342	No Hit
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCTT	3163	0.31614192903548227	Illumina Single End Adapter 2 (95% over 24bp)
AGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	3112	0.31104447776111943	No Hit
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	2427	0.24257871064467768	TruSeq Adapter, Index 22 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	2315	0.23138430784607694	TruSeq Adapter, Index 22 (96% over 25bp)
TGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCC	2278	0.22768615692153926	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG	2177	0.21759120439780108	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1596	0.15952023988005998	No Hit
ACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	1442	0.144127936031984	Illumina Single End Adapter 2 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	1205	0.12043978010994504	Illumina Single End Adapter 2 (95% over 22bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	1101	0.11004497751124437	No Hit
GCCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	1084	0.10834582708645678	TruSeq Adapter, Index 22 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.33323338330834584	0.0
2	0.0	0.0	0.0	0.9997001499250375	0.0
3	0.0	0.0	0.0	1.8467766116941529	0.0
4	0.0	0.0	0.0	2.4267866066966515	0.0
5	0.0	0.0	0.0	5.452373813093454	0.0
6	0.0	0.0	0.0	6.107046476761619	0.0
7	0.0	0.0	0.0	7.730134932533733	0.0
8	0.0	0.0	0.0	9.95632183908046	0.0
9	0.0	0.0	0.0	11.616591704147925	0.0
10	0.0	0.0	0.0	13.110544727636182	0.0
11	0.0	0.0	0.0	14.64447776111944	0.0
12	0.0	0.0	0.0	15.679160419790104	0.0
13	0.0	0.0	0.0	16.14062968515742	0.0
14	0.0	0.0	0.0	16.423788105947025	0.0
15	0.0	0.0	0.0	17.284457771114443	0.0
16	0.0	0.0	0.0	18.4431784107946	0.0
17	0.0	0.0	0.0	19.718740629685158	0.0
18	0.0	0.0	0.0	20.397801099450273	0.0
19	0.0	0.0	0.0	21.062368815592205	0.0
20	0.0	0.0	0.0	21.856671664167916	0.0
21	0.0	0.0	0.0	22.676661669165416	0.0
22	0.0	0.0	0.0	23.49165417291354	0.0
23	0.0	0.0	0.0	24.115742128935533	0.0
24	0.0	0.0	0.0	24.60199900049975	0.0
25	0.0	0.0	0.0	25.110844577711145	0.0
26	0.0	0.0	0.0	25.736031984007997	0.0
27	0.0	0.0	0.0	26.271664167916043	0.0
28	0.0	0.0	0.0	26.87646176911544	0.0
29	0.0	0.0	0.0	27.417391304347827	0.0
30	0.0	0.0	0.0	27.971714142928537	0.0
31	0.0	0.0	0.0	28.426586706646678	0.0
32	9.995002498750625E-5	0.0	0.0	28.93743128435782	0.0
33	9.995002498750625E-5	0.0	0.0	29.53823088455772	0.0
34	9.995002498750625E-5	0.0	0.0	30.268565717141428	0.0
35	9.995002498750625E-5	0.0	0.0	30.785907046476762	0.0
36	9.995002498750625E-5	0.0	0.0	31.329735132433782	0.0
37	9.995002498750625E-5	0.0	0.0	31.972613693153423	0.0
38	9.995002498750625E-5	0.0	0.0	33.404997501249376	0.0
39	9.995002498750625E-5	0.0	0.0	34.00649675162419	0.0
40	9.995002498750625E-5	0.0	0.0	34.50974512743628	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAGCG	35	1.01939804E-7	46.000004	31
CCATCGT	35	1.01939804E-7	46.000004	41
GATATCG	35	1.01939804E-7	46.000004	10
CTTCGAG	25	3.416837E-5	46.0	28
TTAGGCG	25	3.416837E-5	46.0	1
CGAACGT	25	3.416837E-5	46.0	37
CGTATCA	20	6.311062E-4	46.0	43
CGCGGAT	20	6.311062E-4	46.0	3
TCCGAAC	25	3.416837E-5	46.0	35
TTTCGTG	20	6.311062E-4	46.0	31
GACGTTA	20	6.311062E-4	46.0	19
CAGTTCG	20	6.311062E-4	46.0	12
TAGCGCC	25	3.416837E-5	46.0	33
TTCACGG	65	0.0	46.0	1
CTCCCGC	20	6.311062E-4	46.0	37
ATTCGTT	20	6.311062E-4	46.0	42
CGTTAAT	20	6.311062E-4	46.0	21
GGGCGTA	55	1.8189894E-12	46.0	6
ACTACGG	65	0.0	46.0	1
GGACGCG	25	3.416837E-5	46.0	7
>>END_MODULE