FastQCFastQC Report
Sat 14 Jan 2017
SRR2933051.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933051.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences905057
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106491.1766109758832868No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC86430.9549674771865198No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG46270.5112385186789341No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCTT25980.2870537435763714TruSeq Adapter, Index 21 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT24160.26694451288703364TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT23940.26451372675975104TruSeq Adapter, Index 15 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC12420.13722892591295355TruSeq Adapter, Index 21 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA12080.13347225644351682No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG10870.12010293274346256No Hit
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT9820.10850145349961383TruSeq Adapter, Index 15 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGA750.046.00000428
GCAATAG750.046.0000041
GTCATCG253.416576E-546.013
CGGGTAG301.8609608E-646.06
AAGTACG551.8189894E-1246.01
TCGTTTC206.31074E-446.037
GATACGA206.31074E-446.013
TCGTTAT405.6097633E-946.011
ACACGTT253.416576E-546.017
TCGCACC206.31074E-446.025
TACCCCG206.31074E-446.025
CGAACGT301.8609608E-646.029
ACGCCCG206.31074E-446.016
CTCGTAC950.046.029
ATAGGCG206.31074E-446.029
CACGGAT253.416576E-546.018
GCGAATA253.416576E-546.09
TCCGATC206.31074E-446.019
ACGTTCA206.31074E-446.035
CTATCGA206.31074E-446.037
Produced by FastQC (version 0.11.5)