##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933051.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	905057
Sequences flagged as poor quality	0
Sequence length	52
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43246668441877	33.0	31.0	34.0	31.0	34.0
2	32.79759285879232	34.0	31.0	34.0	31.0	34.0
3	32.836421352467305	34.0	31.0	34.0	31.0	34.0
4	36.30313781341949	37.0	37.0	37.0	35.0	37.0
5	36.206886417098595	37.0	37.0	37.0	35.0	37.0
6	36.29335721396553	37.0	37.0	37.0	35.0	37.0
7	36.49270819406954	37.0	37.0	37.0	35.0	37.0
8	36.41722455049793	37.0	37.0	37.0	35.0	37.0
9	38.24313164806195	39.0	39.0	39.0	37.0	39.0
10	37.95291014820061	39.0	38.0	39.0	35.0	39.0
11	37.628193583387564	39.0	37.0	39.0	35.0	39.0
12	37.21665817733027	39.0	35.0	39.0	35.0	39.0
13	37.10849592898569	39.0	35.0	39.0	33.0	39.0
14	38.03463870231378	40.0	36.0	41.0	33.0	41.0
15	38.261268627279826	40.0	36.0	41.0	33.0	41.0
16	38.46763794987498	40.0	36.0	41.0	34.0	41.0
17	38.485857796801746	40.0	36.0	41.0	35.0	41.0
18	38.472329367100635	40.0	36.0	41.0	35.0	41.0
19	38.44379746248026	40.0	36.0	41.0	35.0	41.0
20	38.28737968989798	40.0	35.0	41.0	34.0	41.0
21	38.24028873319581	40.0	35.0	41.0	34.0	41.0
22	38.18566565420741	40.0	35.0	41.0	34.0	41.0
23	38.16133017036496	40.0	35.0	41.0	34.0	41.0
24	38.113768525076324	40.0	35.0	41.0	34.0	41.0
25	37.97961785832273	40.0	35.0	41.0	34.0	41.0
26	37.88494978769293	40.0	35.0	41.0	34.0	41.0
27	37.795568676889964	40.0	35.0	41.0	34.0	41.0
28	37.797704453973616	40.0	35.0	41.0	34.0	41.0
29	37.75581427468104	40.0	35.0	41.0	34.0	41.0
30	37.69060180739998	40.0	35.0	41.0	34.0	41.0
31	37.57485882104663	40.0	35.0	41.0	34.0	41.0
32	37.48019406512518	40.0	35.0	41.0	34.0	41.0
33	37.346521821277555	40.0	35.0	41.0	33.0	41.0
34	37.22568081347362	40.0	35.0	41.0	33.0	41.0
35	37.10930029821326	40.0	35.0	41.0	33.0	41.0
36	36.98243978003595	39.0	35.0	41.0	33.0	41.0
37	36.86891212376679	39.0	35.0	41.0	32.0	41.0
38	36.79100763819295	39.0	35.0	41.0	32.0	41.0
39	36.77429266885953	39.0	35.0	41.0	32.0	41.0
40	36.72929992254632	39.0	35.0	41.0	32.0	41.0
41	36.58036013201379	39.0	35.0	41.0	32.0	41.0
42	36.615183353092675	39.0	35.0	41.0	32.0	41.0
43	36.568620539921795	39.0	35.0	41.0	32.0	41.0
44	36.56762612741518	39.0	35.0	41.0	32.0	41.0
45	36.494676025929856	39.0	35.0	41.0	32.0	41.0
46	36.435410145438354	38.0	35.0	41.0	32.0	41.0
47	36.332906104256416	38.0	35.0	41.0	32.0	41.0
48	36.22282684957964	38.0	35.0	41.0	31.0	41.0
49	36.18382599107017	38.0	35.0	41.0	31.0	41.0
50	36.05166193952425	38.0	35.0	41.0	31.0	41.0
51	35.99148782894337	38.0	35.0	40.0	31.0	41.0
52	35.74998038797556	37.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	5.0
12	4.0
13	4.0
14	6.0
15	18.0
16	46.0
17	89.0
18	171.0
19	331.0
20	597.0
21	944.0
22	1545.0
23	2453.0
24	3466.0
25	5162.0
26	6909.0
27	8251.0
28	9061.0
29	10267.0
30	11985.0
31	14734.0
32	18773.0
33	25993.0
34	66925.0
35	106591.0
36	58489.0
37	67094.0
38	110248.0
39	372831.0
40	2064.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.487362674395094	22.469524018929192	26.595783469991392	13.447329836684318
2	35.411913282809806	24.41116968323542	26.525180182021685	13.651736851933084
3	31.679220203810367	24.40199899011885	29.88695739605351	14.03182341001727
4	28.185959558348262	27.694609289801637	29.450410305649257	14.669020846200848
5	25.88267921246949	31.126216359853576	26.73168651256219	16.25941791511474
6	26.926922834694388	39.639492319268285	23.62116419186858	9.812420654168744
7	86.14264073975451	1.9176692738689387	8.868944165947559	3.0707458204289892
8	83.23718837598074	2.0583233984157903	9.580170088734743	5.124318136868728
9	77.70041002942357	3.775342326505402	12.640640313262038	5.8836073308089984
10	48.01763866806179	22.00612779084632	17.09019431925282	12.886039221839066
11	31.98240552804961	22.80132632530327	27.809077218340946	17.407190928306175
12	30.396207089719212	19.83510430834743	30.94766406977682	18.82102453215654
13	27.065809114785033	23.660167260183613	32.55264585545441	16.721377769576947
14	18.104384585722226	25.200401742652673	35.951989764180595	20.743223907444502
15	14.702720381147264	29.13286124520334	38.24598892666429	17.918429446985108
16	23.927001282792133	26.365190258735083	32.79760280291739	16.910205655555398
17	22.995347254371822	25.50016186825802	27.831064783765	23.673426093605155
18	20.965530347812347	26.16619726713345	33.08443556593673	19.78383681911747
19	21.373570946360285	28.473786733874217	27.27297838699662	22.879663932768874
20	17.868598331375814	35.75752687399799	27.18281831973014	19.191056474896058
21	21.82691255909849	29.066125116981585	31.478459367752524	17.628502956167402
22	19.61931679441184	25.462263702728116	31.89290840245421	23.025511100405833
23	19.47468501983853	26.30309472221087	31.607512013055533	22.614708244895073
24	20.436281913735822	28.597425355530092	30.71707085852051	20.249221872213575
25	21.846690318952287	26.051950319151167	29.57449088841918	22.52686847347736
26	19.16475978861	30.272899938898878	26.530594205668812	24.03174606682231
27	22.55625888756178	27.889624631376808	29.14844037447365	20.405676106587762
28	17.83998134924099	30.264613168010413	27.36380139593418	24.531604086814422
29	21.453565908003586	28.78680569290111	26.429274620272537	23.330353778822772
30	25.11101510733578	28.16319856097461	25.521155021175463	21.204631310514145
31	22.5744897835164	29.88938818218079	27.287452613481804	20.24866942082101
32	26.53932293767133	27.550088005506833	25.244597854057808	20.665991202764026
33	24.102901806184583	29.898116914183305	26.333921509915946	19.665059769716162
34	20.436834365128384	27.775709154230064	31.289189520660027	20.498266959981525
35	22.999987846069363	30.976723013025698	26.73478024036055	19.288508900544386
36	26.09669888194887	29.553055774387694	25.53165159763418	18.818593746029254
37	23.374881361063444	29.7716055452861	28.406498154259896	18.447014939390556
38	22.784421312690803	28.390035102761484	29.354394253621596	19.471149330926117
39	23.18119190283043	28.813654830579733	27.060395091137906	20.944758175451934
40	27.34413412635889	25.437292899784214	28.312802398081004	18.90577057577589
41	21.38859762423803	26.012284309165057	27.78034974592761	24.818768320669307
42	22.10634247345747	26.902062522028995	30.049930556859955	20.941664447653572
43	21.344180532275868	26.9631636460466	31.211625345144007	20.481030476533522
44	23.005401869716493	27.163593011268905	27.51484160666124	22.316163512353366
45	21.07823043189545	26.431042464728744	26.22785084254362	26.26287626083219
46	23.964236506650963	25.552534260273106	27.92962211219846	22.55360712087747
47	20.19132496627284	25.703353490443142	30.736848618374314	23.368472924909703
48	20.94122248653952	27.27607211479498	29.673158707131154	22.109546691534344
49	25.443922316494984	24.71092980884077	28.23766900869227	21.607478865971977
50	22.1513120168122	25.416299746866773	28.73830046063397	23.694087775687056
51	20.114644712984926	25.145046113117736	27.472081868876767	27.26822730502057
52	21.422518139741477	25.843123692761893	30.057333405520314	22.677024761976316
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	878.0
1	1851.5
2	2825.0
3	7712.5
4	12600.0
5	7347.5
6	2095.0
7	2500.0
8	2905.0
9	4044.5
10	5184.0
11	6550.5
12	7917.0
13	8275.0
14	7734.5
15	6836.0
16	6139.5
17	5443.0
18	5144.0
19	4845.0
20	4347.0
21	3849.0
22	3934.5
23	4020.0
24	4107.0
25	4194.0
26	4937.5
27	5681.0
28	6497.5
29	7314.0
30	8381.0
31	9448.0
32	11275.5
33	13103.0
34	15248.0
35	17393.0
36	17528.5
37	17664.0
38	20685.0
39	26741.0
40	29776.0
41	38578.0
42	47380.0
43	57997.0
44	68614.0
45	79586.0
46	90558.0
47	92246.5
48	93935.0
49	88258.0
50	82581.0
51	71740.5
52	60900.0
53	56721.0
54	52542.0
55	46752.0
56	40962.0
57	41729.0
58	42496.0
59	42971.5
60	43447.0
61	34590.0
62	25733.0
63	23193.0
64	16900.5
65	13148.0
66	11681.0
67	10214.0
68	7997.5
69	5781.0
70	4436.5
71	3092.0
72	3016.5
73	2941.0
74	2329.0
75	1717.0
76	1413.0
77	1109.0
78	733.0
79	357.0
80	385.0
81	413.0
82	260.0
83	107.0
84	82.0
85	57.0
86	30.5
87	4.0
88	5.0
89	3.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	905057.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.957442394832423
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.28265205208717	15.212698413949221
2	12.61614234143837	5.5403643741427855
3	5.351216644190869	3.524970936212684
4	2.71678691801423	2.3861476900532708
5	1.6397668207416956	1.800254275369664
6	1.056150451400328	1.3914217618139375
7	0.7181252133248215	1.103773510269956
8	0.5243050017551018	0.9209917498688127
9	0.43710143922852135	0.8637866705302742
>10	3.6825587768351604	17.410456533040374
>50	1.0800423276281228	17.20277952264512
>100	0.8845435527006827	27.8167247785468
>500	0.006061977517080635	0.9584706806049264
>1k	0.003536153551630371	1.7272659921745068
>5k	5.051647930900529E-4	0.95869252314181
>10k+	5.051647930900529E-4	1.181200587635906
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10649	1.1766109758832868	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	8643	0.9549674771865198	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	4627	0.5112385186789341	No Hit
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCTT	2598	0.2870537435763714	TruSeq Adapter, Index 21 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	2416	0.26694451288703364	TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	2394	0.26451372675975104	TruSeq Adapter, Index 15 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC	1242	0.13722892591295355	TruSeq Adapter, Index 21 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	1208	0.13347225644351682	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCG	1087	0.12010293274346256	No Hit
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT	982	0.10850145349961383	TruSeq Adapter, Index 15 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.29688737836401463	0.0
2	0.0	0.0	0.0	1.0507625486571563	0.0
3	0.0	0.0	0.0	1.6195665024412826	0.0
4	0.0	0.0	0.0	2.288143177722508	0.0
5	0.0	0.0	0.0	4.092117955001729	0.0
6	0.0	0.0	0.0	5.7727855814606155	0.0
7	0.0	0.0	0.0	7.206838906278831	0.0
8	0.0	0.0	0.0	9.785571516490121	0.0
9	0.0	0.0	0.0	11.103278578034312	0.0
10	0.0	0.0	0.0	12.450265563384406	0.0
11	0.0	0.0	0.0	14.627034540365965	0.0
12	0.0	0.0	0.0	15.767515195175552	0.0
13	0.0	0.0	0.0	16.23433662189232	0.0
14	0.0	0.0	0.0	16.428910002353444	0.0
15	0.0	0.0	0.0	16.763364075411825	0.0
16	0.0	0.0	0.0	17.384429930932527	0.0
17	0.0	0.0	0.0	18.112008414939613	0.0
18	0.0	0.0	0.0	18.874391336678244	0.0
19	0.0	0.0	0.0	19.338560996710704	0.0
20	0.0	0.0	0.0	19.89178582122452	0.0
21	0.0	0.0	0.0	20.445673587409413	0.0
22	0.0	0.0	0.0	21.048619037254007	0.0
23	0.0	0.0	0.0	21.603390725667	0.0
24	1.1049027851284505E-4	0.0	0.0	22.004912397782682	0.0
25	1.1049027851284505E-4	0.0	0.0	22.448641356290267	0.0
26	1.1049027851284505E-4	0.0	0.0	22.82320340044881	0.0
27	1.1049027851284505E-4	0.0	0.0	23.289803846608557	0.0
28	1.1049027851284505E-4	0.0	0.0	23.674199525554744	0.0
29	1.1049027851284505E-4	0.0	0.0	24.124778881330126	0.0
30	1.1049027851284505E-4	0.0	0.0	25.058753205599206	0.0
31	1.1049027851284505E-4	0.0	0.0	25.507122755804332	0.0
32	1.1049027851284505E-4	0.0	0.0	25.846880362231328	0.0
33	1.1049027851284505E-4	0.0	0.0	26.243650952370956	0.0
34	1.1049027851284505E-4	0.0	0.0	26.68660647892895	0.0
35	1.1049027851284505E-4	0.0	0.0	27.134092106905975	0.0
36	1.1049027851284505E-4	0.0	0.0	27.502687676024824	0.0
37	1.1049027851284505E-4	0.0	0.0	27.888740709148706	0.0
38	1.1049027851284505E-4	0.0	0.0	28.32153113008352	0.0
39	1.1049027851284505E-4	0.0	0.0	29.51261633245199	0.0
40	1.1049027851284505E-4	0.0	0.0	29.92397163935531	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTAGA	75	0.0	46.000004	28
GCAATAG	75	0.0	46.000004	1
GTCATCG	25	3.416576E-5	46.0	13
CGGGTAG	30	1.8609608E-6	46.0	6
AAGTACG	55	1.8189894E-12	46.0	1
TCGTTTC	20	6.31074E-4	46.0	37
GATACGA	20	6.31074E-4	46.0	13
TCGTTAT	40	5.6097633E-9	46.0	11
ACACGTT	25	3.416576E-5	46.0	17
TCGCACC	20	6.31074E-4	46.0	25
TACCCCG	20	6.31074E-4	46.0	25
CGAACGT	30	1.8609608E-6	46.0	29
ACGCCCG	20	6.31074E-4	46.0	16
CTCGTAC	95	0.0	46.0	29
ATAGGCG	20	6.31074E-4	46.0	29
CACGGAT	25	3.416576E-5	46.0	18
GCGAATA	25	3.416576E-5	46.0	9
TCCGATC	20	6.31074E-4	46.0	19
ACGTTCA	20	6.31074E-4	46.0	35
CTATCGA	20	6.31074E-4	46.0	37
>>END_MODULE