FastQCFastQC Report
Sat 14 Jan 2017
SRR2933050.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933050.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences561375
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119702.132264529058116No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC96991.7277221108884435No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAAT60051.0696949454464484No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG39440.7025606769093743No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTTA25620.45637942551770205No Hit
CTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCTT15830.2819861946114451Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCG14460.2575818303273213No Hit
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCAGCAAGCGTCG12370.2203518147405923No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTAT10240.18240926297038523No Hit
CCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT10110.1800935203740815No Hit
GGGGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCT9440.1681585393008239No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCAGCA9390.16726786907147628No Hit
GCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGCT9350.16655533288799823No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGC9340.1663771988421287No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG7970.1419728345580049No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCAGCAAGCGT7730.1376976174571365No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA7140.127187708750835No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACCTGTCTCTTATACACATCT6590.11739033622801158No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCC6460.11507459363170787No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.10830549988866621No Hit
GGGGTTGGGGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCG5950.1059897572923625No Hit
GCTTTACCACCAAAGTAATCCCAAAGGCCACCAAACAAGAGGCCACCCATCC5860.10438655087953684No Hit
TGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG5720.10189267423736362No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGCTGTCTCTTATACACATCTGA5690.10135827209975506No Hit
CGCTGTCTCTTATACACATCTGACGCAGCAAGCGTCGTATGCCGTCTTCTGC5660.10082386996214653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGG850.046.0000042
TCGTTAG301.8596893E-646.01
GTCGAAC206.308681E-446.09
TTTCGAC206.308681E-446.024
TACGGGC253.4149067E-546.04
TATGCGC206.308681E-446.023
GCGTTAG301.8596893E-646.01
CGCCTCT206.308681E-446.035
CGCTATA206.308681E-446.027
CATAGCG301.8596893E-646.01
CGTAGAG253.4149067E-546.039
GCGATGA206.308681E-446.09
AGTTCGG206.308681E-446.020
TCGTAAG253.4149067E-546.01
CGCACTT1350.046.035
TATACGG206.308681E-446.02
CTTAGGC253.4149067E-546.029
ATTTGCG301.8596893E-646.01
TAATGCG206.308681E-446.01
TCGAACT206.308681E-446.010
Produced by FastQC (version 0.11.5)