FastQCFastQC Report
Sat 14 Jan 2017
SRR2933049.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933049.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences767701
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262813.4233379922652176No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC145051.8894074646248995No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG59490.7749110656362307No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCG28260.36811206446259676No Hit
GGGGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT26010.33880377907544734Illumina PCR Primer Index 5 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCTT21040.27406503313138836Illumina PCR Primer Index 5 (96% over 25bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCGCATGATTTCG16570.21583923949558487No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT16500.2149274261724291Illumina PCR Primer Index 5 (95% over 24bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA16010.20854473291033881No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT15770.20541851580237616Illumina PCR Primer Index 5 (95% over 24bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC13930.1814508513079962No Hit
CGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC13090.1705090914301271Illumina PCR Primer Index 5 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT12510.1629540667525508Illumina PCR Primer Index 5 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCT11760.15318463829016765No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA11640.15162152973618634No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC11530.15018868022837015No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCGCATGATTT11430.14888608976671908No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT11290.14706246312040755No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG11130.1449783183817658No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGT8750.11397666539446998No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAA206.310137E-446.00000433
CGTATAA206.310137E-446.00000428
GATAACG405.6079443E-946.0000041
ATCACGG206.310137E-446.0000042
GTTCGCG206.310137E-446.0000041
GCTAGCG206.310137E-446.0000041
CGTAACC206.310137E-446.00000435
ACCGTGC206.310137E-446.00000436
CCGGTAT206.310137E-446.00000442
CGCACGA206.310137E-446.00000440
CGGTATT206.310137E-446.00000443
TGGCGTG253.4160883E-546.01
GCGTTAG453.092282E-1046.01
GTTACCG301.8605897E-646.038
ACTCGGC301.8605897E-646.028
CCGGTGA253.4160883E-546.027
ATAACGG453.092282E-1046.02
CGGTAGT1650.046.012
GACGTAG351.0189797E-745.9999961
CGTTAGG700.045.9999962
Produced by FastQC (version 0.11.5)