##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933049.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	767701
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40500924187933	33.0	31.0	34.0	31.0	34.0
2	32.75535788021639	34.0	31.0	34.0	31.0	34.0
3	32.730705053139175	34.0	31.0	34.0	31.0	34.0
4	36.24185587878614	37.0	37.0	37.0	35.0	37.0
5	36.093776092515185	37.0	35.0	37.0	35.0	37.0
6	36.20153158586481	37.0	36.0	37.0	35.0	37.0
7	36.49138141021049	37.0	37.0	37.0	35.0	37.0
8	36.44570737826315	37.0	37.0	37.0	35.0	37.0
9	38.25988373077539	39.0	39.0	39.0	37.0	39.0
10	37.94035177758008	39.0	38.0	39.0	35.0	39.0
11	37.59677009669129	39.0	37.0	39.0	35.0	39.0
12	37.29958799063698	39.0	37.0	39.0	35.0	39.0
13	37.225417187160105	39.0	37.0	39.0	34.0	39.0
14	37.89361352922557	40.0	37.0	41.0	32.0	41.0
15	38.21552661778479	40.0	37.0	41.0	33.0	41.0
16	38.49469780552585	41.0	37.0	41.0	33.0	41.0
17	38.56406465538016	41.0	37.0	41.0	35.0	41.0
18	38.4691904791058	40.0	37.0	41.0	35.0	41.0
19	38.36760014641117	40.0	37.0	41.0	35.0	41.0
20	38.11417205396372	40.0	35.0	41.0	34.0	41.0
21	38.08053916824389	40.0	35.0	41.0	34.0	41.0
22	38.05801737916194	40.0	35.0	41.0	34.0	41.0
23	38.040875288686614	40.0	35.0	41.0	34.0	41.0
24	37.9767565758023	40.0	35.0	41.0	34.0	41.0
25	37.77877715412641	40.0	35.0	41.0	34.0	41.0
26	37.693112292415925	40.0	35.0	41.0	34.0	41.0
27	37.600499413182995	39.0	35.0	41.0	34.0	41.0
28	37.5167063739659	39.0	35.0	41.0	34.0	41.0
29	37.44176313434527	39.0	35.0	41.0	34.0	41.0
30	37.3722829591208	39.0	35.0	41.0	34.0	41.0
31	37.18665600279275	39.0	35.0	41.0	33.0	41.0
32	37.053321540547685	39.0	35.0	41.0	33.0	41.0
33	36.91377502439101	39.0	35.0	41.0	33.0	41.0
34	36.65491512971847	39.0	35.0	41.0	32.0	41.0
35	36.582363446185425	39.0	35.0	41.0	32.0	41.0
36	36.510908543821095	39.0	35.0	41.0	32.0	41.0
37	36.37401410184434	39.0	35.0	41.0	31.0	41.0
38	36.36280010055999	39.0	35.0	41.0	31.0	41.0
39	36.30835833221528	39.0	35.0	41.0	31.0	41.0
40	36.21973268238546	39.0	35.0	41.0	31.0	41.0
41	36.0516568299377	39.0	35.0	41.0	30.0	41.0
42	36.11332146239226	38.0	35.0	41.0	31.0	41.0
43	36.08527278198153	38.0	35.0	41.0	31.0	41.0
44	36.059166263949116	38.0	35.0	41.0	31.0	41.0
45	35.9954279074796	38.0	35.0	41.0	31.0	41.0
46	35.91926023282502	38.0	35.0	41.0	31.0	41.0
47	35.81367355259405	38.0	35.0	41.0	31.0	41.0
48	35.69993916902544	38.0	35.0	41.0	30.0	41.0
49	35.676510776982184	37.0	35.0	41.0	30.0	41.0
50	35.565752812618456	37.0	35.0	40.0	30.0	41.0
51	35.47511205534446	37.0	35.0	40.0	29.0	41.0
52	35.22721476199718	36.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	1.0
14	9.0
15	20.0
16	35.0
17	100.0
18	200.0
19	363.0
20	575.0
21	981.0
22	1541.0
23	2390.0
24	4063.0
25	6739.0
26	8650.0
27	9148.0
28	9404.0
29	10056.0
30	11266.0
31	13665.0
32	17066.0
33	23969.0
34	57956.0
35	73521.0
36	67288.0
37	64894.0
38	85516.0
39	296429.0
40	1853.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.99135470710602	21.18598256352408	25.62950940535443	16.19315332401547
2	40.70126260093448	21.83284898678001	24.592647397880164	12.873241014405348
3	31.361037695665374	22.248375344046707	33.169424033575574	13.221162926712351
4	28.346843367404755	25.19092719691651	32.69580214171924	13.766427293959497
5	23.503812030986023	29.44922567510007	32.47175658231525	14.575205711598658
6	25.182460358915776	35.58143079141489	30.212673944673774	9.023434904995565
7	82.69808167502713	1.5754831633669881	13.130893407719931	2.595541753885953
8	80.35433065737833	2.0059893109426716	13.62600804219351	4.0136719894854895
9	75.1181775196333	3.817111088822341	15.829860844260981	5.234850547283383
10	44.98404977979708	25.392437941333927	19.406383474816366	10.217128804052619
11	27.138951232315705	23.271560151673633	33.28483354847786	16.304655067532803
12	27.64500762666715	17.908013666779123	36.10376956653697	18.34320914001675
13	27.407545385508158	19.25984204788062	38.8353017646193	14.497310801991922
14	16.317159935964654	22.099228736187655	43.07992304295553	18.503688284892164
15	13.05482212475951	29.327303207889532	41.37444135151576	16.2434333158352
16	26.570370495805008	27.13191724382279	31.900961442019742	14.396750818352459
17	23.146771985447458	26.077991301300894	29.222835452865116	21.55240126038653
18	20.470469622939138	25.441806119830506	36.70257040175798	17.385153855472378
19	19.763553779401093	28.7416585363312	30.62689771147882	20.867889972788888
20	14.448463659680005	39.68107375136935	29.329908388812832	16.540554200137812
21	19.55943785406037	29.34111066678303	37.09738557068442	14.002065908472177
22	15.943186214424626	25.4952123287582	39.51343035895486	19.04817109786232
23	17.467217054556397	26.996057058672584	36.9320868411009	18.604639045670122
24	19.78739118484931	31.11211265844385	30.704271584900894	18.39622457180595
25	21.114991383364096	28.528554736805084	31.20498735835957	19.15146652147125
26	15.244216172702654	32.13868420127107	28.210071368931395	24.407028257094883
27	20.367825494561036	27.587172610169848	33.15535605658974	18.88964583867938
28	14.725654909919356	31.265036778641687	28.66741088001709	25.341897431421867
29	20.87023463561986	31.291739883105528	27.761589472984927	20.076436008289686
30	24.582747710371617	26.43307746114698	27.321053378854526	21.663121449626875
31	20.00127653865242	29.711437135030433	30.677828998529378	19.609457327787773
32	24.350105053920732	27.853682618623655	27.13595527425391	20.660257053201704
33	24.144165501933696	26.49221506810594	29.978077402530413	19.38554202742995
34	17.175176273054223	28.808481427013906	34.48074185131972	19.535600448612158
35	19.583796295693247	32.29603712903852	29.732408841463016	18.38775773380522
36	23.15849529960232	28.784644021565686	27.792981903110714	20.263878775721277
37	16.403391424525953	33.94550743062729	30.042425371335973	19.608675773510782
38	20.21802759147116	28.83583582670858	33.309452508203066	17.636684073617204
39	18.656742664136168	30.56776010451986	29.289918861640146	21.48557836970383
40	24.422529083588532	28.30047114696998	30.88376855051641	16.393231218925074
41	17.048173703043243	28.54561867185271	29.581308347911488	24.824899277192554
42	16.922213205401583	29.51422493913646	34.49272568356691	19.07083617189505
43	16.905019011307786	29.40637044891175	33.61985981521451	20.068750724565945
44	22.487530952805844	27.66793321879221	28.64448528789203	21.200050540509913
45	18.701551776016963	26.7272023873878	27.595509189124414	26.97573664747083
46	21.398956104004032	26.997620167226565	29.202645300709523	22.400778428059883
47	16.035149101017193	28.24393872093432	33.67339628318838	22.04751589486011
48	16.483109960779004	32.309584069839694	30.499895141467835	20.70741082791347
49	22.106002206588244	28.267255090197878	30.408453291059928	19.21828941215395
50	18.775278396146415	27.579617585492265	30.02184444204189	23.623259576319427
51	18.46018176347302	28.13660526689427	28.292004308969247	25.111208660663458
52	18.126067310059515	29.329778129766666	30.274937768740696	22.269216791433124
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	769.0
1	1525.5
2	2282.0
3	15852.0
4	29422.0
5	16378.0
6	3334.0
7	3784.0
8	4234.0
9	5976.5
10	7719.0
11	9994.0
12	12269.0
13	12913.5
14	12969.5
15	12381.0
16	11130.0
17	9879.0
18	8721.5
19	7564.0
20	6946.5
21	6329.0
22	6070.5
23	5812.0
24	6060.0
25	6308.0
26	6662.0
27	7016.0
28	7493.5
29	7971.0
30	8874.0
31	9777.0
32	10342.5
33	10908.0
34	12849.5
35	14791.0
36	15213.0
37	15635.0
38	17231.0
39	21523.0
40	24219.0
41	29163.5
42	34108.0
43	40310.0
44	46512.0
45	54831.0
46	63150.0
47	67052.0
48	70954.0
49	71878.5
50	72803.0
51	62256.5
52	51710.0
53	46975.0
54	42240.0
55	38919.5
56	35599.0
57	34152.0
58	32705.0
59	35493.0
60	38281.0
61	27124.5
62	15968.0
63	13239.0
64	9166.5
65	7823.0
66	6657.0
67	5491.0
68	4344.5
69	3198.0
70	2668.0
71	2138.0
72	1665.0
73	1192.0
74	1046.5
75	901.0
76	815.5
77	730.0
78	539.0
79	348.0
80	261.0
81	174.0
82	137.5
83	101.0
84	65.5
85	30.0
86	27.0
87	24.0
88	12.5
89	1.0
90	1.0
91	3.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	767701.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.54049984625587
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.98130636565352	24.171893017636407
2	12.154176242011474	8.39622645237128
3	5.080876249307973	5.264880159242014
4	2.7442068279478398	3.791451020753067
5	1.7296622126471657	2.987169869500701
6	1.138161805204478	2.358760659460774
7	0.8614995489617645	2.0829637526924327
8	0.6811210534914972	1.8821009314723758
9	0.5708544564800592	1.7745838439655566
>10	4.909528984792462	32.22774731765356
>50	0.10020523620814634	2.2217899657658426
>100	0.03630076297026505	2.4191400866801582
>500	0.004915728318890041	1.0720387556133624
>1k	0.00605012716171082	3.2452420761117464
>5k	3.7813294760692624E-4	0.7769930016013514
>10k+	7.562658952138525E-4	5.327019089479361
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26281	3.4233379922652176	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGC	14505	1.8894074646248995	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGACG	5949	0.7749110656362307	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCG	2826	0.36811206446259676	No Hit
GGGGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	2601	0.33880377907544734	Illumina PCR Primer Index 5 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCTT	2104	0.27406503313138836	Illumina PCR Primer Index 5 (96% over 25bp)
GGGGTTGGGGATTTAGCCTGTCTCTTATACACATCTGACGCGCATGATTTCG	1657	0.21583923949558487	No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	1650	0.2149274261724291	Illumina PCR Primer Index 5 (95% over 24bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCACTGTCTCTTA	1601	0.20854473291033881	No Hit
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	1577	0.20541851580237616	Illumina PCR Primer Index 5 (95% over 24bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCC	1393	0.1814508513079962	No Hit
CGCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	1309	0.1705090914301271	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCT	1251	0.1629540667525508	Illumina PCR Primer Index 5 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCT	1176	0.15318463829016765	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCATA	1164	0.15162152973618634	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTC	1153	0.15018868022837015	No Hit
GGGGTTGGGGATTTAGCTCCTGTCTCTTATACACATCTGACGCGCATGATTT	1143	0.14888608976671908	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTT	1129	0.14706246312040755	No Hit
GGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1113	0.1449783183817658	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGT	875	0.11397666539446998	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3025904616510855E-4	0.0	0.0	0.28565808824008304	0.0
2	1.3025904616510855E-4	0.0	0.0	0.8429062877344174	0.0
3	1.3025904616510855E-4	0.0	0.0	1.3984611196286054	0.0
4	1.3025904616510855E-4	0.0	0.0	2.04011718103793	0.0
5	1.3025904616510855E-4	0.0	0.0	4.0556154023506545	0.0
6	1.3025904616510855E-4	0.0	0.0	5.656629338766004	0.0
7	1.3025904616510855E-4	0.0	0.0	6.769171852062196	0.0
8	1.3025904616510855E-4	0.0	0.0	8.86113213347384	0.0
9	1.3025904616510855E-4	0.0	0.0	9.938113927166956	0.0
10	1.3025904616510855E-4	0.0	0.0	11.425020939141671	0.0
11	1.3025904616510855E-4	0.0	0.0	13.776587499560376	0.0
12	1.3025904616510855E-4	0.0	0.0	15.216861773007981	0.0
13	1.3025904616510855E-4	0.0	0.0	15.896944253036013	0.0
14	1.3025904616510855E-4	0.0	0.0	16.510724878566005	0.0
15	1.3025904616510855E-4	0.0	0.0	16.99633060266953	0.0
16	1.3025904616510855E-4	0.0	0.0	17.712234320392966	0.0
17	1.3025904616510855E-4	0.0	0.0	18.55045128246544	0.0
18	1.3025904616510855E-4	0.0	0.0	19.50616190417884	0.0
19	1.3025904616510855E-4	0.0	0.0	20.08034377967464	0.0
20	1.3025904616510855E-4	0.0	0.0	20.828291222754693	0.0
21	1.3025904616510855E-4	0.0	0.0	21.500948937151314	0.0
22	1.3025904616510855E-4	0.0	0.0	22.195359912257505	0.0
23	1.3025904616510855E-4	0.0	0.0	22.852126023021984	0.0
24	2.605180923302171E-4	0.0	0.0	23.34489599466459	0.0
25	2.605180923302171E-4	0.0	0.0	23.881302746772505	0.0
26	2.605180923302171E-4	0.0	0.0	24.357790337644474	0.0
27	2.605180923302171E-4	0.0	0.0	25.049596131827364	0.0
28	2.605180923302171E-4	0.0	0.0	25.5288191626688	0.0
29	2.605180923302171E-4	0.0	0.0	26.152629734753503	0.0
30	2.605180923302171E-4	0.0	0.0	27.42617242910977	0.0
31	2.605180923302171E-4	0.0	0.0	28.00269896743654	0.0
32	3.9077713849532564E-4	0.0	0.0	28.465639617507335	0.0
33	3.9077713849532564E-4	0.0	0.0	28.977818187028543	0.0
34	3.9077713849532564E-4	0.0	0.0	29.556428870093956	0.0
35	3.9077713849532564E-4	0.0	0.0	30.062355005399237	0.0
36	3.9077713849532564E-4	0.0	0.0	30.487911309220646	0.0
37	3.9077713849532564E-4	0.0	0.0	30.998135993049377	0.0
38	3.9077713849532564E-4	0.0	0.0	31.567758801929397	0.0
39	3.9077713849532564E-4	0.0	0.0	33.219964543487635	0.0
40	3.9077713849532564E-4	0.0	0.0	33.749337307102635	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAA	20	6.310137E-4	46.000004	33
CGTATAA	20	6.310137E-4	46.000004	28
GATAACG	40	5.6079443E-9	46.000004	1
ATCACGG	20	6.310137E-4	46.000004	2
GTTCGCG	20	6.310137E-4	46.000004	1
GCTAGCG	20	6.310137E-4	46.000004	1
CGTAACC	20	6.310137E-4	46.000004	35
ACCGTGC	20	6.310137E-4	46.000004	36
CCGGTAT	20	6.310137E-4	46.000004	42
CGCACGA	20	6.310137E-4	46.000004	40
CGGTATT	20	6.310137E-4	46.000004	43
TGGCGTG	25	3.4160883E-5	46.0	1
GCGTTAG	45	3.092282E-10	46.0	1
GTTACCG	30	1.8605897E-6	46.0	38
ACTCGGC	30	1.8605897E-6	46.0	28
CCGGTGA	25	3.4160883E-5	46.0	27
ATAACGG	45	3.092282E-10	46.0	2
CGGTAGT	165	0.0	46.0	12
GACGTAG	35	1.0189797E-7	45.999996	1
CGTTAGG	70	0.0	45.999996	2
>>END_MODULE